Luciferase expression controlled by a viral gene promoter in a mammalian circadian pacemaker.


:Retinal light exposure induces several immediate-early genes in the hypothalamic suprachiasmatic nucleus (SCN), which contains the major circadian pacemaker of mammals. Clock-controlled and light-induced genes expressed in the SCN such as c- and contain upstream regulatory elements similar to those of the major immediate-early gene (IE-1) of the human cytomegalovirus. IE-1 expression is critical for viral reactivation from latency and increases in response to agents acting through depolarization or the cAMP response element. To test whether IE-1 could be under circadian control, bioluminescence was imaged in individual SCN cells of brain slice cultures from transgenic mice containing the IE-1 enhancer/promoter upstream from the firefly luciferase gene. A small percentage of the cells in neonatal and adult cultures displayed circadian transgene expression, particularly ones near the dorsomedial edge of the SCN. Single-cell bioluminescence imaging revealed that the circadian pacemaker can regulate exogenous viral genes and could play a role in viral diseases.






Sigworth LA,Liao L,Chandler TR,Geusz ME




Has Abstract


2003-03-03 00:00:00












  • Cells over-expressing EAAT2 protect motoneurons from excitotoxic death in vitro.

    abstract::Amyotrophic lateral sclerosis is an incurable disease in which cerebral and spinal motoneurons degenerate, causing paralysis and death within 2-5 years. One of the pathogenic factors of motoneuron death is a chronic excess of glutamate, which exceeds its removal by astrocytes, i.e. excitotoxicity. Extra glutamate upta...


    pub_type: 杂志文章


    authors: Wisman LA,van Muiswinkel FL,de Graan PN,Hol EM,Bär PR

    更新日期:2003-10-27 00:00:00

  • Fast responses to target changes are not impaired in children with spastic hemiplegia.

    abstract::Humans are able to correct an ongoing movement very quickly in response to a suddenly moving target. Such fast responses possibly bypass the motor cortex and if so, one would expect that damage to the motor cortex would not greatly affect them. A group of children with congenital spastic hemiplegia were asked to move ...


    pub_type: 杂志文章,随机对照试验


    authors: Smits-Engelsman BC,Rameckers EE,Duysens J

    更新日期:2009-01-07 00:00:00

  • Visually induced gamma-band responses to coherent and incoherent motion: a replication study.

    abstract::The present study was based on earlier findings that the observation of a coherently moving long bar induced gamma-band activity in humans. The power in the EEG-gamma-band was reduced during the presentation of two incoherently moving short bars. The present study demonstrates the replicability of this cortical activi...


    pub_type: 杂志文章


    authors: Müller MM,Junghöfer M,Elbert T,Rochstroh B

    更新日期:1997-07-28 00:00:00

  • MRI visualization of focal induced neocortical malformations of the rat.

    abstract::In an attempt to determine whether small focal malformations of the neocortex can be visualized in vivo, focal microgyria were induced in the neocortex of otherwise normal rats by freezing injury to the developing cortical plate, and in adulthood the malformation was visualized using MRI. Induced microgyria of varying...


    pub_type: 杂志文章


    authors: Rosen GD,Burstein D

    更新日期:1997-12-22 00:00:00

  • Early emotional prosody perception based on different speaker voices.

    abstract::Decoding verbal and nonverbal emotional expressions is an important part of speech communication. Although various studies have tried to specify the brain regions that underlie different emotions conveyed in speech, few studies have aimed to specify the time course of emotional speech decoding. We used event-related p...


    pub_type: 杂志文章


    authors: Paulmann S,Kotz SA

    更新日期:2008-01-22 00:00:00

  • Tannic acid inhibits electrogenic Na+/HCO3- co-transporter activity in embryonic neural stem cell-derived radial glial-like cells.

    abstract::Self-renewing neural stem cells and progenitor cells are cell populations that generate radial glial cells and neurons through asymmetric division. Regulation of intracellular pH in stem cells with high metabolic activity is critical for both cell signaling and proliferation. We have recently found that a S0859-inhibi...


    pub_type: 杂志文章


    authors: Nordström T,Zhenyu G,Andersson LC,Åkerman KEO

    更新日期:2020-01-08 00:00:00

  • Nuclear translocation of DJ-1 protects adult neuronal stem cells in an MPTP mouse model of Parkinson's disease.

    abstract::DJ-1, originally identified as an oncogene product, is involved in multiple processes, including cellular transformation, the oxidative stress response, and transcriptional regulation. We had previously found that DJ-1 was closely associated with the proliferation and differentiation of cultured neuronal stem cells. I...


    pub_type: 杂志文章


    authors: Sun Y,Wang Y,Zhao X,Pu X

    更新日期:2018-03-07 00:00:00

  • Cortical categorization failure in 2-month-old infants at risk for dyslexia.

    abstract::Cortical auditory categorization was assessed in 2-month-old infants at genetic risk for dyslexia and controls. Manipulated /bAk/ and /dAk/ speech stimuli were used as frequently presented standards. The neuroelectric brain responses of the control infants were highly sensitive to the phoneme boundary that separated t...


    pub_type: 杂志文章


    authors: van Leeuwen T,Been P,van Herten M,Zwarts F,Maassen B,van der Leij A

    更新日期:2007-06-11 00:00:00

  • Long-term potentiation in the perirhinal-hippocampal pathway is NMDA dependent.

    abstract::The putative memory mechanism known as long-term potentiation (LTP) has been described in both NMDA-dependent and non-NMDA-dependent forms. The aim of the present study was to determine which of these forms described the LTP generated between perirhinal cortex (PRC) and hippocampus, two structures which have previousl...


    pub_type: 杂志文章


    authors: Liu P,Bilkey DK

    更新日期:1996-05-17 00:00:00

  • PKC and tyrosine kinase involvement in amyloid beta (25-35)-induced chemotaxis of microglia.

    abstract::Microglia are activated by amyloid beta (Abeta) in vivo and in vitro, and Abeta-activated microglia may be involved in the pathogenesis of Alzheimer's disease (AD). We investigated the mechanism of microglial chemotaxis induced by Abeta (25-35), an active fragment of Abeta. Abeta (25-35) 0.1 and 1 nM stimulated microg...


    pub_type: 杂志文章


    authors: Nakai M,Hojo K,Taniguchi T,Terashima A,Kawamata T,Hashimoto T,Maeda K,Tanaka C

    更新日期:1998-10-26 00:00:00

  • Sexual receptivity is reduced in the female mu-opioid receptor knockout mouse.

    abstract::Activation of mu-opioid receptors is critical to steroid regulation of female sexual behavior, lordosis, in rodents. Estrogen treatment activates mu-opioid receptors in the medial preoptic area inhibiting lordosis, but ultimately appears important for progesterone facilitation of lordosis. We investigated the role of ...


    pub_type: 杂志文章


    authors: Sinchak K,Shahedi K,Dewing P,Micevych P

    更新日期:2005-10-17 00:00:00

  • On the origin of Alzheimer's disease: a hypothesis.

    abstract::There is no unifying hypothesis to account for the anatomical distribution of neuropathology, the involvement of beta-amyloid precursor protein (beta APP) and the role of increasing age in triggering the Alzheimer disease process. We report here that layer II pre-alpha neurones in transentorhinal and entorhinal cortex...


    pub_type: 杂志文章


    authors: Roberts GW,Nash M,Ince PG,Royston MC,Gentleman SM

    更新日期:1993-01-01 00:00:00

  • Effects of body weight-supported treadmill training at different speeds on the motor function and depressive behaviors after spinal cord injury in rats.

    abstract::Spinal cord injury (SCI) causes motor dysfunction and depression, which hinders the recovery of motor function. Body weight-supported treadmill training (BWSTT) should be considered an effective method for functional rehabilitation after SCI, as it is an efficacious intervention in healthcare with no side effects. Bec...


    pub_type: 杂志文章


    authors: Liu CH,Zhao BL,Li WT,Zhou XH,Jin Z,An LB

    更新日期:2020-12-16 00:00:00

  • High-frequency SEP components generated in the somatosensory cortex of the monkey.

    abstract::To investigate the origin of high-frequency somatosensory evoked potential (SEP) components, we recorded median nerve SEPs from the scalp and the depth in six monkeys. Laminar field potentials were analyzed in area 3b (N10; corresponding to human N20) and area 1 (P12; corresponding to human P25). After digital filteri...


    pub_type: 杂志文章


    authors: Shimazu H,Kaji R,Tsujimoto T,Kohara N,Ikeda A,Kimura J,Shibasaki H

    更新日期:2000-08-21 00:00:00

  • Audiogenic seizure susceptibility in thyroid hormone receptor beta-deficient mice.

    abstract::As early-onset hypothyroidism produces audiogenic seizure susceptibility (AGS) in rodents, the role of TR alpha 1 and TR beta thyroid hormone receptors in AGS was investigated. AGS occurs in mice lacking specifically TR beta (Thrb(tm1/tm1)) and is marked by early onset and persistence, thereby differing from mouse str...


    pub_type: 杂志文章


    authors: Ng L,Pedraza PE,Faris JS,Vennström B,Curran T,Morreale de Escobar G,Forrest D

    更新日期:2001-08-08 00:00:00

  • The T. cruzi trans-sialidase induces PC12 cell differentiation via MAPK/ERK pathway.

    abstract::The trans-sialidase (TS) of Trypanosoma cruzi induces survival and differentiation of neuronal and glial cells. This mechanism underlying survival is via phosphatidylinositol 3-kinase (PI3K) but how TS promotes neuronal differentiation remained to be determined. Here we show that TS-induced neurite outgrowth in PC12 c...


    pub_type: 杂志文章


    authors: Chuenkova MV,Pereira MA

    更新日期:2001-12-04 00:00:00

  • Metabotropic purinoreceptors in rat dorsal horn neurones: predominant dendritic location.

    abstract::Elevations of cytosolic free Ca2+ concentration ([Ca2+]i) induced by addition of ATP have been compared in rat dorsal horn neurones in slices and after their isolation. ATP application induced in neurones in situ a rise of [Ca2+]i by 201 +/- 12 nM. In Ca2+-free external solution the rise was 156 +/- 14 nM (n = 45 of 7...


    pub_type: 杂志文章


    authors: Kruglikov I,Shutov L,Potapenko E,Voitenko N,Kostyuk P

    更新日期:2001-11-16 00:00:00

  • Effects of baicalin on diabetic neuropathic pain involving transient receptor potential vanilloid 1 in the dorsal root ganglia of rats.

    abstract::Diabetic peripheral neuropathy is the most common complication of diabetes mellitus and leads to sensory symptoms, including diabetic neuropathic pain (DNP). DNP is a major public health problem because it has a considerable impact on life quality of diabetes mellitus patients. Therefore, development of novel effectiv...


    pub_type: 杂志文章


    authors: Li P,Xiong DL,Sun WP,Xu SY

    更新日期:2018-12-05 00:00:00

  • P2X3 receptor in injured human sensory neurons.

    abstract::The ATP-gated cation channel P2X3 is expressed selectively by rat sensory neurones, and may play a role in nociception by binding ATP released from damaged or inflamed tissues. However, the distribution of this channel in human sensory neurons is not known. Using a specific antibody, we have demonstrated intense P2X3 ...


    pub_type: 杂志文章


    authors: Yiangou Y,Facer P,Birch R,Sangameswaran L,Eglen R,Anand P

    更新日期:2000-04-07 00:00:00

  • Three time windows for amnestic effect of antibodies to cell adhesion molecule L1 in chicks.

    abstract::The L1/NgCAM cell adhesion molecule is involved in neurite outgrowth, axonal fasciculation and cell migration in the nervous system. We studied the effects of antibodies against L1 injected intracranially at various times before and after training 2-day-old chicks in a visual categorization task. Memory retention was ...


    pub_type: 杂志文章


    authors: Tiunova A,Anokhin KV,Schachner M,Rose SP

    更新日期:1998-05-11 00:00:00

  • Microwave antigen retrieval of beta-amyloid precursor protein immunoreactivity.

    abstract::Formalin fixation reduces beta-amyloid precursor protein (beta APP) immunoreactivity which restricts its study in archival tissue. Formic acid pretreatment has previously been used in an attempt to overcome this problem, but makes the sections very friable. In the present study, a microwave antigen retrieval method ha...


    pub_type: 杂志文章


    authors: Sherriff FE,Bridges LR,Jackson P

    更新日期:1994-05-09 00:00:00

  • Synaptic plasticity dynamics in the hippocampal-prefrontal pathway in vivo.

    abstract::We examined the effect of long-term potentiation (LTP) on paired-pulse responses across varied stimulus intensities and interstimulus intervals (ISIs), at ascending synapses from the intermediate and ventral hippocampus to the medial prefrontal cortex in urethane-anesthetized rats. LTP significantly shifted the median...


    pub_type: 杂志文章


    authors: Takita M,Izaki Y,Kuramochi M,Yokoi H,Ohtomi M

    更新日期:2010-01-06 00:00:00

  • Lipid peroxidation inhibition in spinal cord injury: cyclosporin-A vs methylprednisolone.

    abstract::To compare the effectiveness of cyclosporin-A (CsA) with methylprednisolone (MP) or a combination of both upon inhibition of lipid peroxidation (LP) after spinal cord (SC) injury, rats were treated with either CsA, MP, CSA+MP or vehicle starting 1 h after SC contusion at T9 level. LP was assessed 24h after injury by t...


    pub_type: 杂志文章


    authors: Diaz-Ruiz A,Rios C,Duarte I,Correa D,Guizar-Sahagun G,Grijalva I,Madrazo I,Ibarra A

    更新日期:2000-06-05 00:00:00

  • NMDA receptors increase OH radicals in vivo by using nitric oxide synthase and protein kinase C.

    abstract::Prolonged activation of brain N-methyl-D-aspartic acid (NMDA) receptors increases intraneuronal (Ca2+) and nitric oxide (NO) synthesis, and may be responsible for neuronal death in acute brain insults and chronic neurodegenerative diseases. NO can be converted in vitro to toxic hydroxyl (OH) radical. Using microdialys...


    pub_type: 杂志文章


    authors: Hammer B,Parker WD Jr,Bennett JP Jr

    更新日期:1993-10-25 00:00:00

  • Interindividual and interspecies variations of the extrastriate visual cortex.

    abstract::Functional homology between human and macaque visual cortices has provided an important cue to functional subdivisions of the human visual cortex, but it is unclear beyond V1. We estimated the sizes and the visual field eccentricity functions of the extrastriate visual areas of human brains using MRI and fMRI measurem...


    pub_type: 杂志文章


    authors: Ejima Y,Takahashi S,Yamamoto H,Fukunaga M,Tanaka C,Ebisu T,Umeda M

    更新日期:2003-08-26 00:00:00

  • Altered protein mannosylation in developing cerebral cortex by streptomycin.

    abstract::Our research objective was to characterize the biochemical effect of streptomycin during postnatal rat cerebral cortex development using a sensitive method that preserves the in situ topological relationship. We found a decrease in the mannosylation of asparagine-linked oligosaccharides without affecting polypeptide s...


    pub_type: 杂志文章


    authors: Alperin MD,Calandria JM,Carminatti H,Idoyaga-Vargas VP

    更新日期:2000-11-09 00:00:00

  • Histamine H3-receptor activation inhibits dopamine synthesis in rat striatum.

    abstract::Unilateral 6-hydroxydopamine lesion to rat substantia nigra pars compacta resulted in a modest, but significant, decrease in the specific binding of N-alpha-[methyl-3H]histamine (19 +/- 5% reduction) to synaptosomal membranes from ipsilateral striata. Dopamine synthesis was assessed in striatal slices by determining [...


    pub_type: 杂志文章


    authors: Molina-Hernández A,Nuñez A,Arias-Montaño JA

    更新日期:2000-01-17 00:00:00

  • In vivo transfer of a marker gene to study motoneuronal development.

    abstract::Adenovirus vectors containing a marker gene (lacZ from Escherichia coli) are potent for transferring the gene to neurones after intraparenchymal injections. Expression of the marker gene may lead to the synthesis of an enormous amount of beta-galactosidase which diffuses throughout the entire neurone, providing a 'Gol...


    pub_type: 杂志文章


    authors: Lisovoski F,Cadusseau J,Akli S,Caillaud C,Vigne E,Poenaru L,Stratford-Perricaudet L,Pericaudet M,Kahn A,Peschanski M

    更新日期:1994-05-09 00:00:00

  • Brain activity assessed with PET during recall of word lists and narratives.

    abstract::This study investigated the functional neuroanatomy involved in retrieval of structured versus unstructured verbal information. We compared cerebral blood flow using PET with the [15O]water method while subjects engaged in recall of novel and practised narratives and lists of unrelated words. Left orbital frontal cort...


    pub_type: 临床试验,杂志文章


    authors: Paradiso S,Crespo Facorro B,Andreasen NC,O'Leary DS,Watkins LG,Boles Ponto L,Hichwa RD

    更新日期:1997-09-29 00:00:00

  • Auditory sensory memory in 2-year-old children: an event-related potential study.

    abstract::Auditory sensory memory is assumed to play an important role in cognitive development, but little is known about it in young children. The aim of this study was to estimate the duration of auditory sensory memory in 2-year-old children. We recorded the mismatch negativity in response to tone stimuli presented with dif...


    pub_type: 杂志文章


    authors: Glass E,Sachse S,von Suchodoletz W

    更新日期:2008-03-26 00:00:00