Luciferase expression controlled by a viral gene promoter in a mammalian circadian pacemaker.

Abstract:

:Retinal light exposure induces several immediate-early genes in the hypothalamic suprachiasmatic nucleus (SCN), which contains the major circadian pacemaker of mammals. Clock-controlled and light-induced genes expressed in the SCN such as c- and contain upstream regulatory elements similar to those of the major immediate-early gene (IE-1) of the human cytomegalovirus. IE-1 expression is critical for viral reactivation from latency and increases in response to agents acting through depolarization or the cAMP response element. To test whether IE-1 could be under circadian control, bioluminescence was imaged in individual SCN cells of brain slice cultures from transgenic mice containing the IE-1 enhancer/promoter upstream from the firefly luciferase gene. A small percentage of the cells in neonatal and adult cultures displayed circadian transgene expression, particularly ones near the dorsomedial edge of the SCN. Single-cell bioluminescence imaging revealed that the circadian pacemaker can regulate exogenous viral genes and could play a role in viral diseases.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Sigworth LA,Liao L,Chandler TR,Geusz ME

doi

10.1097/00001756-200303030-00029

subject

Has Abstract

pub_date

2003-03-03 00:00:00

pages

443-7

issue

3

eissn

0959-4965

issn

1473-558X

journal_volume

14

pub_type

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