Abstract:
:Peripheral sensorineural hearing loss is a very common inner ear disorder affecting nearly 10% of the population. At present there is no cure for this disorder but gene therapy has been suggested as a potentially effective method for clinical treatment in the future. Thus we investigated the effectiveness of adenoviral (Ad) and adeno-associated viral (AAV) vectors to transduce the cochlea of guinea pigs. After direct injection into the basal turn of the cochlea, we found that both Ad and AAV vectors coding for the reporter genes lacZ or GFP readily transduced spiral ganglion cells. In addition, some transgene expression was detected in the stria vascularis after AAV-GFP injection. Gene expression persisted at least 8 weeks after viral vector injection. Present findings will help to develop future gene therapy protocols in the inner ear by using Ad and AAV coding for neurotrophins such as NT-3, BDNF, GDNF and VEGF.
journal_name
Neuroreportjournal_title
Neuroreportauthors
Li Duan M,Bordet T,Mezzina M,Kahn A,Ulfendahl Mdoi
10.1097/00001756-200207190-00016subject
Has Abstractpub_date
2002-07-19 00:00:00pages
1295-9issue
10eissn
0959-4965issn
1473-558Xjournal_volume
13pub_type
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