In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies.

Abstract:

:Cholinergic basal forebrain neurons (CBFN) expressing the low-affinity neurotrophin receptor p75 (p75(NTR)) were previously selectively labeled in vivo with carbocyanine 3 (Cy3)-tagged anti-p75(NTR), but the applied 192IgG-conjugates recognized p75(NTR) only in rat. The antibody ME 20.4 raised against human p75(NTR) had been shown to cross-react with the receptor in monkey, raccoon, sheep, cat, dog, pig and rabbit. Hence, for in vivo labeling of rabbit CBFN in the present study, ME 20.4 was fluorochromated with Cy3-N-hydroxysuccinimide ester and purified Cy3-ME 20.4 was injected intracerebroventricularly. Two days post-injection, clusters of Cy3-ME 20.4 were found in CBFN displaying choline acetyltrans-ferase-immunoreactivity. Following photoconversion, electron microscopy revealed fluorochromated antibodies in secondary lysosomes. In conclusion, Cy3-ME 20.4 might become an appropriate marker for CBFN in live and fixed tissues of various mammalian species.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Härtig W,Varga C,Kacza J,Grosche J,Seeger J,Luiten PG,Brauer K,Harkany T

doi

10.1097/00001756-200208070-00009

subject

Has Abstract

pub_date

2002-08-07 00:00:00

pages

1395-8

issue

11

eissn

0959-4965

issn

1473-558X

journal_volume

13

pub_type

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