Abstract：
:The genetic analysis of the large and complex herpesviruses has been a constant challenge to herpesvirologists. Elegant methods have been developed to produce mutants in infected cells that rely on the cellular recombination machinery. Bacterial artificial chromosomes (BACs), single copy F-factor-based plasmid vectors of intermediate insert capacity, have now enabled the cloning of complete herpesvirus genomes. Infectious virus genomes can be shuttled between Escherichia coli and eukaryotic cells. Herpesvirus BAC DNA engineering in E. coli by homologous recombination requires neither restriction sites nor cloning steps and allows the introduction of a wide variety of DNA modifications. Such E. coli-based technology has provided a safe, fast and effective approach to the systematic mining of the information stored in herpesvirus genomes as a result of their intimate co-evolution with their specific hosts for millions of years. Use of this technique could lead to new developments in clinical virology and basic virology research, and increase the usage of viral genomes as investigative tools and vectors.
journal_name
Trends Microbioljournal_title
Trends in microbiologyauthors
Wagner M,Ruzsics Z,Koszinowski UHdoi
10.1016/s0966-842x(02)02394-6subject
Has Abstractpub_date
2002-07-01 00:00:00pages
318-24issue
7eissn
0966-842Xissn
1878-4380pii
S0966842X02023946journal_volume
10pub_type
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journal_title：Trends in microbiology
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abstract：:The existence of peptidoglycan (PG) in chlamydiae has long been debated. Genome sequencing of members of the Chlamydiaceae family and Protochlamydia amoebophila has uncovered a nearly complete pathway for PG synthesis in these organisms. The recent use of microarray and proteomic analysis methods has revealed that PG ...
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doi：10.1016/j.tim.2017.05.015
更新日期：2017-12-01 00:00:00
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journal_title：Trends in microbiology
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journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2012.12.002
更新日期：2013-03-01 00:00:00
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journal_title：Trends in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.tim.2008.01.012
更新日期：2008-05-01 00:00:00
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更新日期：2013-07-01 00:00:00
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journal_title：Trends in microbiology
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journal_title：Trends in microbiology
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