Abstract:
:To study the function of astrocytes in the adult brain, we have targeted the expression of E. coli nitroreductase (NTR) to the astrocytes of transgenic mice under the control of the GFAP promoter. The astrocytes expressing NTR were selectively ablated after administration of the prodrug CB1954, resulting in motor discoordination. Histological examination showed that the region most affected in the brain was the cerebellum, in which the Bergmann glia were eliminated and the granular neurons had degenerated. Specific effects were also noted on the dendrites of the Purkinje cells, and the junction between these neurons and granular layer was disrupted. Astrocyte ablation was associated with a dramatic decrease in the expression of glutamate transporters, which may account for the degeneration of granular neurons since the excitotoxic effects of glutamate result in a similar phenotype. These results provide the first evidence that astrocytes are important for the survival of neurons in the adult brain in vivo.
journal_name
Gliajournal_title
Gliaauthors
Cui W,Allen ND,Skynner M,Gusterson B,Clark AJdoi
10.1002/glia.1061subject
Has Abstractpub_date
2001-06-01 00:00:00pages
272-82issue
4eissn
0894-1491issn
1098-1136pii
10.1002/glia.1061journal_volume
34pub_type
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