Abstract:
:Oligodendrocytes were studied in the anterior medullary velum (AMV) of the rat using the monoclonal antibody Rip, an oligodendrocyte marker of unknown function. Confocal microscopic imaging of double immunofluorescent labelling with antibodies to Rip and carbonic anhydrase II (CAII) revealed two biochemically and morphologically distinct populations of oligodendrocyte which were either Rip+CAII+ or Rip+CAII-. Double immunofluorescent labelling with Rip and myelin basic protein (MBP) or glial fibrillary acidic protein (GFAP) provided direct evidence that Rip-labelled cells were phenotypically oligodendrocytes and confirmed that Rip did not recognise astrocytes. Oligodendrocytes which were Rip+CAII+ supported numerous myelin sheaths for small diameter axons, whilst Rip+CAII- oligodendrocytes supported fewer myelin sheaths for large diameter axons. Morphologically, Rip+CAII+ oligodendrocytes corresponded to types I or II of classical nomenclature, whilst Rip+CAII- oligodendrocytes corresponded to types III and IV. The results demonstrated a biochemical difference between oligodendrocytes which myelinated small and large diameter fibres.
journal_name
Gliajournal_title
Gliaauthors
Butt AM,Ibrahim M,Ruge FM,Berry Mdoi
10.1002/glia.440140304subject
Has Abstractpub_date
1995-07-01 00:00:00pages
185-97issue
3eissn
0894-1491issn
1098-1136journal_volume
14pub_type
杂志文章相关文献
GLIA文献大全abstract::The purpose of the present study was to elucidate the expression and regulation of the L-cystine transporter, system x(c) (-), in Müller cells. In this study, newly developed conditionally immortalized rat Müller cell lines (TR-MUL) from transgenic rats harboring the temperature-sensitive SV 40 large T-antigen gene we...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10253
更新日期:2003-09-01 00:00:00
abstract::Short term and chronic dBcAMP effects on the expression of glial fibrillary acidic protein (GFAP) in astroglial primary cultures are investigated. Short (48 h) and long (more than 7 days) treatments with the cAMP derivative induce both cell shape changes and an increase in GFAP immunolabelling. Such effects are only a...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440040310
更新日期:1991-01-01 00:00:00
abstract::Differentiation of oligodendrocyte precursor cells (OPCs) is the most important event for the myelination of central nervous system (CNS) axons during development and remyelination in demyelinating diseases, while the underlying molecular mechanisms remain largely unknown. Here we show that NMDA receptor (NMDAR) is a ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22469
更新日期:2013-05-01 00:00:00
abstract::Even though the immune privileged status of the central nervous system (CNS) limits access of systemic immune cells through the blood brain barrier (BBB), an immune response can occur in this compartment with or without major breach of the BBB. In this review, we consider properties of resident cells of the CNS, that ...
journal_title:Glia
pub_type: 杂志文章,评审
doi:
更新日期:2000-02-15 00:00:00
abstract::We have previously shown that cultured astrocytes from neonatal rat cerebral cortex are depolarized by GABA. The underlying ionic mechanism, activation of a Cl- conductance and responses to an agonist and antagonists were found to be similar to those of the neuronal GABAA receptor (Kettenmann et al.: Brain Research 40...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440010205
更新日期:1988-01-01 00:00:00
abstract::Insulin-like growth factor II (IGFII) acts as a potent mitogen for several tumor types and has been reported to positively influence astrocytoma cell growth and motility. In the central nervous system, IGFII bioavailability is mainly modulated by insulin-like growth factor binding protein 2 (IGFBP2), which sequestrate...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20719
更新日期:2008-11-15 00:00:00
abstract::Regeneration in the adult central nervous system (CNS) is thought to be hampered by the lesion-induced activation of astrocytes and meningeal cells and the consecutive formation of a glial scar. The substrate properties of reactive astrocytes differ significantly from their neonatal counterparts, which promote axon gr...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199903)26:1<36::aid-glia4>
更新日期:1999-03-01 00:00:00
abstract::C-erbB receptor/neuregulin signalling plays a significant role in Schwann cell function. In vivo, Schwann cells up-regulate expression of c-erbB receptors in the first month after injury, but receptor expression is down-regulated with time to levels that are not detectable immunohistochemically. The inability of chron...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Interactions between astrocytes and endothelial cells are believed to play an important role in the control of blood-brain barrier permeability and transport. Astrocytes and endothelial cells respond to a variety of stimuli with an increase of intracellular free calcium ([Ca2+]i) that is propagated to adjacent cells a...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Astrocytes release a variety of transmitter molecules, which mediate communication between glial cells in the brain and modulate synaptic transmission. ATP is a major glia-derived transmitter, but the mechanisms and kinetics of ATP release from astrocytes remain largely unknown. Here, we combined epifluorescence and t...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20590
更新日期:2008-01-01 00:00:00
abstract::NG2 cells (polydendrocytes) are the fourth major non-neuronal cell type in the central nervous system parenchyma. They exhibit diverse properties, ranging from their well-established role as oligodendrocyte precursors to their ability to respond to neurotransmitters released by synaptic and non-synaptic mechanisms. Th...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.22664
更新日期:2014-08-01 00:00:00
abstract::The biologically active lipid metabolite, platelet-activating factor (PAF), is thought to contribute to inflammatory processes and tissue damage in a variety of central nervous system (CNS) injuries. In previous studies, we found that after contusion spinal cord injury, treatment with a PAF antagonist led to significa...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10065
更新日期:2002-05-01 00:00:00
abstract::Much current knowledge of oligodendrocyte biology, the myelin-forming cells in the central nervous system, comes from cell culture studies mainly from postnatal rat tissue but mouse cells have been much more difficult to produce in large quantities. We have developed a high yield protocol for production of oligodendro...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20702
更新日期:2008-09-01 00:00:00
abstract::External application of bradykinin (BK) to mouse neuroblastoma X mouse fibroblast hybrid NL308 cells and mouse neuroblastoma X rat glioma hybrid NG108-15 cells produced a transient outward (hyperpolarizing) current. In NG108-15 cells, BK also induced an inward (depolarizing) current associated with a decrease in input...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440030102
更新日期:1990-01-01 00:00:00
abstract::Syndecan-1, -2, -3, and -4 are heparan sulfate proteoglycans that are differentially expressed during development and wound repair. To determine whether syndecans are also involved in brain injury, we examined the expression of syndecan core proteins genes in cryo-injured mouse brain, using in situ hybridization. All ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10078
更新日期:2002-07-01 00:00:00
abstract::Assembly of the extracellular matrix (ECM) has been tightly linked to compact myelin formation in the peripheral nervous system. We recently demonstrated that myelination of dorsal root ganglion (DRG) axons by Schwann cells may occur in the absence of basal lamina. We have now determined whether laminin deposition occ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1068
更新日期:2001-07-01 00:00:00
abstract::When central nervous system (CNS) homeostasis is altered, microglial cells become rapidly activated, proliferate and release a broad range of molecules. Among the plethora of molecules involved in the regulation of microglial activation, cytokines are considered crucial. Although production of interleukin-10 (IL-10) h...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23573
更新日期:2019-04-01 00:00:00
abstract::We investigated the effect of receptor blockade induced by an angiotensin II type-1 receptor antagonist (AT(1)-RB) on glial and vascular changes in oxygen-induced retinopathy (OIR), a model of retinopathy of prematurity (ROP). OIR was induced in Sprague-Dawley rats by exposure to 80% oxygen from postnatal (P) days 0-1...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20680
更新日期:2008-08-01 00:00:00
abstract::ATP stimulates arachidonic acid mobilization and eicosanoid production in cultured astrocytes via P2Y-purinergic receptors. To assist in determining the mechanism of phospholipase A2 activation and the role of calcium in eicosanoid production, cultures were pretreated with pertussis toxin (PTx). ATP-evoked eicosanoid ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440070305
更新日期:1993-03-01 00:00:00
abstract::Astrocytes express gap junction proteins and multiple types of P2Y receptors (P2YRs) that contribute to the propagation of intercellular Ca(2+) waves (ICW). To gain access to the role played by gap junctional communication in ICW propagation generated by P2YR activation, we selectively expressed P2Y(1,2,4)R subtypes a...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20071
更新日期:2004-11-15 00:00:00
abstract::Elevated levels of Oncostatin M (OSM), an interleukin-6 family cytokine, have been observed in multiple sclerosis (MS), HIV-associated neurocognitive disorder (HAND), and glioblastoma (GBM); however, its effects within the CNS are not well understood. OSM regulates gene expression primarily by activating the JAK/STAT,...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20989
更新日期:2010-07-01 00:00:00
abstract::VEGF is an important factor in tumor vascularization and used as target for anti-angiogenic treatment strategies in glioma. In this study, we demonstrate for the first time that VEGF is a modulator of the innate immune response with suppressive effects on the immunologic and pro-angiogenic function of microglia/macrop...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23234
更新日期:2018-01-01 00:00:00
abstract::Endothelins are potent vasoactive peptides that bind to their specific receptors, playing an important role in the CNS under physiological and pathophysiological conditions. Astrocytes, which have been shown to express these receptors, also have a considerable role to play under physiological and pathophysiological co...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Glial cell-mediated potassium and glutamate homeostases play important roles in the regulation of neuronal excitability. Diminished potassium and glutamate buffering capabilities of astrocytes result in hyperexcitability of neurons and abnormal synaptic transmission. The role of the different K+ channels in maintainin...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20455
更新日期:2007-02-01 00:00:00
abstract::Microglia are key players of the immune response in the central nervous system (CNS) and, being the resident innate immune cells, they are responsible for the early control of infections and for the recruitment of cells of the adaptive immune system required for pathogen clearance. The innate and adaptive immune respo...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.20928
更新日期:2010-02-01 00:00:00
abstract::Macrophages are critically involved in the pathogenesis of genetically caused demyelination, as it occurs in inherited demyelinating neuropathies. On the basis of the observation that upregulation of the Schwann cell-derived chemokine MCP-1 (CCL2) is a pathologically relevant mechanism for macrophage activation in mic...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20657
更新日期:2008-06-01 00:00:00
abstract::The normal adult vertebrate nervous system is a relative quiescent tissue in terms of cell proliferation. However, astrocytes in many regions of the central nervous system (CNS) retain the capacity to undergo cell division. To examine the mechanisms that regulate the proliferation of astrocytes in the CNS we have util...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/(sici)1098-1136(199804)22:4<379::aid-glia7
更新日期:1998-04-01 00:00:00
abstract::In the present study the distribution of astrocytes in the nerve fiber layer (NFL) has been studied in the sparsely vascularized retinae of the guinea pig and horse and in the richly vascularized retina of the Old World monkey (Cercopithecus aethiops) using immunocytochemical methods. In the guinea pig retina glial fi...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440010109
更新日期:1988-01-01 00:00:00
abstract::Astrocytes support neurons not only physically but also chemically by secreting neurotrophic factors and energy substrates. Moreover, astrocytes establish a glial network and communicate through gap junctions in the brain. Connexin 43 (Cx43) is one of major component proteins in astrocytic gap junctions. Heterozygote ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20399
更新日期:2006-10-01 00:00:00
abstract::Transplantation of oligodendrocyte precursor cells (OPCs) results in efficient remyelination in animal models of demyelination. However, the experiments so far undertaken have not addressed the need for tissue-type matching to achieve graft-mediated remyelination. Examination of MHC expression (main determinant of all...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20425
更新日期:2006-12-01 00:00:00