Gap junction channels coordinate the propagation of intercellular Ca2+ signals generated by P2Y receptor activation.


:Astrocytes express gap junction proteins and multiple types of P2Y receptors (P2YRs) that contribute to the propagation of intercellular Ca(2+) waves (ICW). To gain access to the role played by gap junctional communication in ICW propagation generated by P2YR activation, we selectively expressed P2Y(1,2,4)R subtypes and Cx43 in the human 1321N1 astrocytoma cell line, which lacks endogenous P2 receptors. Fluorescence recovery after photobleaching revealed that 1321N1 cells are poorly dye-coupled and do not propagate ICW. Forced expression of Cx43 in 1321N1 cells (which did not show functional hemichannels) increased dye coupling and allowed short-range ICW transmission that was mainly mediated by intercellular diffusion of Ca(2+) generated in the stimulated cells. Astrocytoma clones expressing each of the P2YR subtypes were also able to propagate ICWs that were likely dependent on IP(3) generation. These waves exhibited properties particular to each P2YR subtype. Co-expression of eGFP-hCx43 and P2Y(1)R modified the properties of P2Y(1)R-generated ICW to those characteristics of P2Y(2)R. Increased coupling in P2Y(4)R clones induced by expression of eGFP-hCx43 abolished the ICWs observed in uncoupled P2Y(4)R clones. No changes in the behavior of ICWs generated in P2Y(2)R clones were observed after forced expression of Cx43. These data indicate that in 1321N1 cells gap junctional communication provides intercellular integration of Ca(2+) signals generated by P2YR activation, thus coordinating the propagation of intercellular calcium waves.






Suadicani SO,Flores CE,Urban-Maldonado M,Beelitz M,Scemes E




Has Abstract


2004-11-15 00:00:00














  • Laminin molecules in freeze-treated nerve segments are associated with migrating Schwann cells that display the corresponding alpha6beta1 integrin receptor.

    abstract::Isolated acellular nerve segments protected from migration of Schwann cells and the acellular nerve segments joined with the distal nerve stumps were prepared by a repeated freeze-thaw procedure in the rat sciatic nerves. The presence of laminin-1 and -2, as well as alpha6 and beta1 integrin chains, was detected by in...


    pub_type: 杂志文章


    authors: Dubový P,Svízenská I,Klusáková I,Zítková A,Houst'Ava L,Haninec P

    更新日期:2001-01-01 00:00:00

  • Repetitive injury and absence of monocytes promote astrocyte self-renewal and neurological recovery.

    abstract::Unlike microglia and NG2 glia, astrocytes are incapable of migrating to sites of injury in the posttraumatic cerebral cortex, instead relying on proliferation to replenish their numbers and distribution in the affected region. However, neither the spectrum of their proliferative repertoire nor their postinjury distrib...


    pub_type: 杂志文章


    authors: Lange Canhos L,Chen M,Falk S,Popper B,Straub T,Götz M,Sirko S

    更新日期:2021-01-01 00:00:00

  • Effect of elevated K(+), hypotonic stress, and cortical spreading depression on astrocyte swelling in GFAP-deficient mice.

    abstract::Glial fibrillary acidic protein (GFAP) is the main component of intermediate filaments in astrocytes. To assess its function in astrocyte swelling, we compared astrocyte membrane properties and swelling in spinal cord slices of 8- to 10-day-old wild-type control (GFAP(+/+)) and GFAP-knockout (GFAP(-/-)) mice. Membrane...


    pub_type: 杂志文章


    authors: Anderová M,Kubinová S,Mazel T,Chvátal A,Eliasson C,Pekny M,Syková E

    更新日期:2001-09-01 00:00:00

  • Simvastatin regulates oligodendroglial process dynamics and survival.

    abstract::Simvastatin, a lipophilic statin that crosses the blood-brain barrier, is being evaluated as a potential therapy for multiple sclerosis (MS) due to its anti-inflammatory properties. We assessed the effects of simvastatin on cultures of rat newborn and human fetal oligodendrocyte progenitor cells (OPCs) and human adult...


    pub_type: 杂志文章


    authors: Miron VE,Rajasekharan S,Jarjour AA,Zamvil SS,Kennedy TE,Antel JP

    更新日期:2007-01-15 00:00:00

  • Glutamate opens Na+/K+ channels in cultured astrocytes.

    abstract::Glial cells from different brain regions and species are depolarized by the neurotransmitter glutamate. The depolarization or, if voltage-clamped at the resting membrane potential, the inward current induced by glutamate could be due either to activation of receptor-coupled ion channels or electrogenic uptake of the t...


    pub_type: 杂志文章


    authors: Sontheimer H,Kettenmann H,Backus KH,Schachner M

    更新日期:1988-01-01 00:00:00

  • Focal adhesion kinase is required for endothelin-induced cell cycle progression of cultured astrocytes.

    abstract::When the brain is damaged, astrocytes often cause hyperplasia resulting in glial scar formation at the injured sites. Endothelins (ETs) have been shown to be involved in the pathophysiologic responses of astrocytes, including proliferation. In this study, we examined the mechanisms underlying the ET-induced astrocytic...


    pub_type: 杂志文章


    authors: Koyama Y,Yoshioka Y,Matsuda T,Baba A

    更新日期:2003-08-01 00:00:00

  • NG2 cells (polydendrocytes): listeners to the neural network with diverse properties.

    abstract::NG2 cells (polydendrocytes) are the fourth major non-neuronal cell type in the central nervous system parenchyma. They exhibit diverse properties, ranging from their well-established role as oligodendrocyte precursors to their ability to respond to neurotransmitters released by synaptic and non-synaptic mechanisms. Th...


    pub_type: 杂志文章,评审


    authors: Hill RA,Nishiyama A

    更新日期:2014-08-01 00:00:00

  • Nitric oxide as a messenger between neurons and satellite glial cells in dorsal root ganglia.

    abstract::Abnormal neuronal activity in sensory ganglia contributes to chronic pain. There is evidence that signals can spread between cells in these ganglia, which may contribute to this activity. Satellite glial cells (SGCs) in sensory ganglia undergo activation following peripheral injury and participate in cellular communic...


    pub_type: 杂志文章


    authors: Belzer V,Hanani M

    更新日期:2019-07-01 00:00:00

  • MiR-409-3p and MiR-1896 co-operatively participate in IL-17-induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling.

    abstract::Th17 cells and interleukin-17 (IL-17) have been found to play an important role in the pathology of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Response to IL-17, reactive astrocytes accompany with immune cells infiltration and axonal damage in MS/EAE. However, the ro...


    pub_type: 杂志文章


    authors: Liu X,Zhou F,Yang Y,Wang W,Niu L,Zuo D,Li X,Hua H,Zhang B,Kou Y,Guo J,Kong F,Pan W,Gao D,Meves JM,Sun H,Xue M,Zhang Q,Wang Y,Tang R

    更新日期:2019-01-01 00:00:00

  • Ultrastructural relationships between noradrenergic nerve fibers and non-neuronal elements in the rat cerebral cortex.

    abstract::Pharmacological and biochemical data suggest that noradrenaline (NA)-containing fibers not only regulate the activity of cortical neurons but also influence the functional state of non-neuronal elements. In the present study, immunocytochemistry with an antiserum against NA, followed by silver-gold intensification of ...


    pub_type: 杂志文章


    authors: Paspalas CD,Papadopoulos GC

    更新日期:1996-06-01 00:00:00

  • Non-myelin-forming perisynaptic schwann cells express protein zero and myelin-associated glycoprotein.

    abstract::Perisynaptic Schwann cells (PSCs) envelop axonal terminals and are physiologically distinct from the nearby myelinating Schwann cells (MSCs), which surround the same innervating motor axons. PSCs have special functions at the neuromuscular synapse, where they detect and can modulate neurotransmitter release. Although ...


    pub_type: 杂志文章


    authors: Georgiou J,Charlton MP

    更新日期:1999-08-01 00:00:00

  • Pure astrocyte cultures derived from cells isolated from mature brain.

    abstract::Enriched preparations of oligodendrocytes, isolated either from adult bovine brain or from 30-day-old rat brain, eventually yield cultures in MEM-15% calf serum that contain, in addition to oligodendrocytes, proliferating astrocytes and variable numbers of fibroblast-like cells. If these cultures are switched to a ser...


    pub_type: 杂志文章


    authors: Norton WT,Farooq M,Chiu FC,Bottenstein JE

    更新日期:1988-01-01 00:00:00

  • Depression of parallel and climbing fiber transmission to Bergmann glia is input specific and correlates with increased precision of synaptic transmission.

    abstract::In the cerebellar cortex, Bergmann glia enclose the synapses of both parallel and climbing fiber inputs to the Purkinje neuron. The glia express Ca(2+)-permeable AMPA receptors, and the GLAST and GLT-1 classes of glutamate transporter, which are activated by glutamate released during synaptic transmission. We have pre...


    pub_type: 杂志文章


    authors: Balakrishnan S,Bellamy TC

    更新日期:2009-03-01 00:00:00

  • beta-catenin mediates insulin-like growth factor-I actions to promote cyclin D1 mRNA expression, cell proliferation and survival in oligodendroglial cultures.

    abstract::By promoting cell proliferation, survival and maturation insulin-like growth factor (IGF)-I is essential to the normal growth and development of the central nervous system. It is clear that IGF-I actions are primarily mediated by the type I IGF receptor (IGF1R), and that phosphoinositide 3 (PI3)-Akt kinases and MAP ki...


    pub_type: 杂志文章


    authors: Ye P,Hu Q,Liu H,Yan Y,D'ercole AJ

    更新日期:2010-07-01 00:00:00

  • Media that support the growth and differentiation of oligodendrocytes do not induce olfactory ensheathing cells to express a myelinating phenotype.

    abstract::The glial cells that ensheath olfactory axons are referred to as ensheathing cells. In vivo, these non-myelinating glial cells express a mixture of astrocyte-specific and Schwann cell-specific phenotypic features with the former cellular phenotype predominating, but in vitro can assemble a myelin sheath when co-cultur...


    pub_type: 杂志文章


    authors: Doucette R,Devon R

    更新日期:1994-04-01 00:00:00

  • Differential expression of sPLA2 following spinal cord injury and a functional role for sPLA2-IIA in mediating oligodendrocyte death.

    abstract::After the initial mechanical insult of spinal cord injury (SCI), secondary mediators propagate a massive loss of oligodendrocytes. We previously showed that following SCI both the total phospholipase activity and cytosolic PLA(2)-IV alpha protein expression increased. However, the expression of secreted isoforms of PL...


    pub_type: 杂志文章


    authors: Titsworth WL,Cheng X,Ke Y,Deng L,Burckardt KA,Pendleton C,Liu NK,Shao H,Cao QL,Xu XM

    更新日期:2009-11-01 00:00:00

  • Astroglia contribute to the pathogenesis of spinocerebellar ataxia Type 1 (SCA1) in a biphasic, stage-of-disease specific manner.

    abstract::Spinocerebellar ataxia type 1 (SCA1) is a fatal, dominantly inherited neurodegenerative disease caused by the expansion of CAG repeats in the Ataxin-1 (ATXN1) gene. SCA1 is characterized by balance and coordination deficits due to the predominant loss of Purkinje neurons in the cerebellum. We previously demonstrated t...


    pub_type: 杂志文章


    authors: Kim JH,Lukowicz A,Qu W,Johnson A,Cvetanovic M

    更新日期:2018-09-01 00:00:00

  • Distribution of Aquaporin 4 in rodent spinal cord: relationship with astrocyte markers and chondroitin sulfate proteoglycans.

    abstract::Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system, as demonstrated by its perturbations in pathological conditions. Aquaporin 4 (AQP4) is the predominant water channel in brain and spinal cord, where it is present mainly on astrocytic endfeet c...


    pub_type: 杂志文章


    authors: Vitellaro-Zuccarello L,Mazzetti S,Bosisio P,Monti C,De Biasi S

    更新日期:2005-08-01 00:00:00

  • Microglia release ATP by exocytosis.

    abstract::Microglia survey the brain environment by sensing several types of diffusible molecules, among which extracellular nucleotides released/leaked from damaged cells have central roles. Microglia sense ATP or other nucleotides by multiple P2 receptors, after which they change into several different phenotypes. However, so...


    pub_type: 杂志文章


    authors: Imura Y,Morizawa Y,Komatsu R,Shibata K,Shinozaki Y,Kasai H,Moriishi K,Moriyama Y,Koizumi S

    更新日期:2013-08-01 00:00:00

  • Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal loss.

    abstract::Microglia contribute to pathophysiology at all stages of multiple sclerosis. Colony-stimulating factor-1 (CSF1) is crucial for microglial proliferation and activation. In this study we measured the CSF1 levels and studied its cellular expression in the mouse spinal cords with experimental autoimmune encephalomyelitis ...


    pub_type: 杂志文章


    authors: Gushchina S,Pryce G,Yip PK,Wu D,Pallier P,Giovannoni G,Baker D,Bo X

    更新日期:2018-10-01 00:00:00

  • Inducible ablation of astrocytes shows that these cells are required for neuronal survival in the adult brain.

    abstract::To study the function of astrocytes in the adult brain, we have targeted the expression of E. coli nitroreductase (NTR) to the astrocytes of transgenic mice under the control of the GFAP promoter. The astrocytes expressing NTR were selectively ablated after administration of the prodrug CB1954, resulting in motor disc...


    pub_type: 杂志文章


    authors: Cui W,Allen ND,Skynner M,Gusterson B,Clark AJ

    更新日期:2001-06-01 00:00:00

  • The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.

    abstract::During axonal ensheathment, noncompact myelin channels formed at lateral edges of the myelinating process become arranged into tight paranodal spirals that resemble loops when cut in cross section. These adhere to the axon, concentrating voltage-dependent sodium channels at nodes of Ranvier and patterning the surround...


    pub_type: 杂志文章


    authors: Jarjour AA,Velichkova AN,Boyd A,Lord KM,Torsney C,Henderson DJ,Ffrench-Constant C

    更新日期:2020-09-01 00:00:00

  • Purinergic P2Y receptors on astrocytes are directly coupled to phospholipase A2.

    abstract::ATP stimulates arachidonic acid mobilization and eicosanoid production in cultured astrocytes via P2Y-purinergic receptors. To assist in determining the mechanism of phospholipase A2 activation and the role of calcium in eicosanoid production, cultures were pretreated with pertussis toxin (PTx). ATP-evoked eicosanoid ...


    pub_type: 杂志文章


    authors: Bruner G,Murphy S

    更新日期:1993-03-01 00:00:00

  • LPS-induced expression of a novel chemokine receptor (L-CCR) in mouse glial cells in vitro and in vivo.

    abstract::There is increasing evidence that chemokines, specialized regulators of the peripheral immune system, are also involved in the physiology and pathology of the CNS. It is known that glial cells (astrocytes and microglia) express various chemokine receptors like CCR1, -3, -5, and CXCR4. We have investigated the possible...


    pub_type: 杂志文章


    authors: Zuurman MW,Heeroma J,Brouwer N,Boddeke HW,Biber K

    更新日期:2003-03-01 00:00:00

  • Zn and Cu alteration in connection with astrocyte metallothionein I/II overexpression in the mouse brain upon physical stress.

    abstract::The distribution of metallothioneins I/II in the mouse brain and their specific area distribution upon physical stress were studied. To induce physical stress, groups of mice were subjected to total darkness for different periods (2 weeks, 1 month, and 2 months). The concentration of metallothioneins, evaluated by imm...


    pub_type: 杂志文章


    authors: Beltramini M,Di Pisa C,Zambenedetti P,Wittkowski W,Mocchegiani E,Musicco M,Zatta P

    更新日期:2004-07-01 00:00:00

  • Capacitative Ca2+ influx in glial cells is inhibited by glycolytic inhibitors.

    abstract::In non-excitable cells, stimulation of phosphoinositide (PI) turnover and inhibition of the endoplasmic reticulum (ER) Ca2+-ATPase are methods commonly used to deplete calcium stores, resulting in a capacitative Ca2+ influx (i.e., Ca2+ release-activated Ca2+ influx). Since this Ca2+ influx in glial cells has not been ...


    pub_type: 杂志文章


    authors: Wu ML,Kao EF,Liu IH,Wang BS,Lin-Shiau SY

    更新日期:1997-11-01 00:00:00

  • Reactive astrocytes in myelin-deficient rat optic nerve reveal an altered distribution of orthogonal arrays of particles (OAP).

    abstract::Reactive astrocytes are a common feature of various pathological conditions within the CNS. Morphological changes of reactive astrocytes include an altered nucleus-cytoplasm relationship, nuclear indentations, an increased amount of intermediate filaments, and an immunologically immature phenotype. Additionally, the n...


    pub_type: 杂志文章


    authors: Rohlmann A,Gocht A,Wolburg H

    更新日期:1992-01-01 00:00:00

  • Sodium-dependent vitamin C transporter 2 (SVCT2) is necessary for the uptake of L-ascorbic acid into Schwann cells.

    abstract::Ascorbic acid has been shown to be an essential component for in vitro myelination and to improve the clinical and pathological phenotype of a mouse model of Charcot-Marie-tooth disease 1A. The mechanism of ascorbic acid uptake into peripheral nerves, however, has not been addressed so far. Hence, we studied the expre...


    pub_type: 杂志文章


    authors: Gess B,Lohmann C,Halfter H,Young P

    更新日期:2010-02-01 00:00:00

  • T- and B-cell responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis and multiple sclerosis.

    abstract::The identification of myelin oligodendrocyte glycoprotein (MOG) as a target for autoantibody-mediated demyelination in experimental autoimmune encephalomyelitis (EAE) resulted in the re-evaluation of the role of B cell responses to myelin autoantigens in the immunopathogenesis of multiple sclerosis. MOG is a central n...


    pub_type: 杂志文章,评审


    authors: Iglesias A,Bauer J,Litzenburger T,Schubart A,Linington C

    更新日期:2001-11-01 00:00:00

  • Functional properties of retinal Müller cells following transplantation to the anterior eye chamber.

    abstract::Two types of glial cells occur in the retina, Müller cells and astrocytes. These cells share several structural features such as extending endfeet onto blood vessels of the retina. Retinal vessels express a tight blood-retinal barrier which is comparable to the blood-brain barrier (BBB) of the CNS. While astrocytes ha...


    pub_type: 杂志文章


    authors: Small RK,Watkins BA,Munro PM,Liu D

    更新日期:1993-02-01 00:00:00