Abstract:
:Astrocytes express gap junction proteins and multiple types of P2Y receptors (P2YRs) that contribute to the propagation of intercellular Ca(2+) waves (ICW). To gain access to the role played by gap junctional communication in ICW propagation generated by P2YR activation, we selectively expressed P2Y(1,2,4)R subtypes and Cx43 in the human 1321N1 astrocytoma cell line, which lacks endogenous P2 receptors. Fluorescence recovery after photobleaching revealed that 1321N1 cells are poorly dye-coupled and do not propagate ICW. Forced expression of Cx43 in 1321N1 cells (which did not show functional hemichannels) increased dye coupling and allowed short-range ICW transmission that was mainly mediated by intercellular diffusion of Ca(2+) generated in the stimulated cells. Astrocytoma clones expressing each of the P2YR subtypes were also able to propagate ICWs that were likely dependent on IP(3) generation. These waves exhibited properties particular to each P2YR subtype. Co-expression of eGFP-hCx43 and P2Y(1)R modified the properties of P2Y(1)R-generated ICW to those characteristics of P2Y(2)R. Increased coupling in P2Y(4)R clones induced by expression of eGFP-hCx43 abolished the ICWs observed in uncoupled P2Y(4)R clones. No changes in the behavior of ICWs generated in P2Y(2)R clones were observed after forced expression of Cx43. These data indicate that in 1321N1 cells gap junctional communication provides intercellular integration of Ca(2+) signals generated by P2YR activation, thus coordinating the propagation of intercellular calcium waves.
journal_name
Gliajournal_title
Gliaauthors
Suadicani SO,Flores CE,Urban-Maldonado M,Beelitz M,Scemes Edoi
10.1002/glia.20071subject
Has Abstractpub_date
2004-11-15 00:00:00pages
217-29issue
3eissn
0894-1491issn
1098-1136journal_volume
48pub_type
杂志文章相关文献
GLIA文献大全abstract::Individuals with neurofibromatosis 1 (NF1) develop low-grade astrocytomas at an increased frequency. To gain insight into the function of the Nf1 gene product as a growth regulator for astrocytes, we examined mice heterozygous for a targeted Nf1 mutation. In our previous studies, we demonstrated increased numbers of p...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/1098-1136(20010315)33:4<314::aid-glia1030>
更新日期:2001-03-15 00:00:00
abstract::The endocannabinoid system exhibits anti-inflammatory properties by regulating cytokine production. Anandamide (AEA) down-regulates proinflammatory cytokines in a viral model of multiple sclerosis (MS). However, little is known about the mechanisms by which AEA exerts these effects. Microglial cells are the main sourc...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20907
更新日期:2010-01-15 00:00:00
abstract::Isolated acellular nerve segments protected from migration of Schwann cells and the acellular nerve segments joined with the distal nerve stumps were prepared by a repeated freeze-thaw procedure in the rat sciatic nerves. The presence of laminin-1 and -2, as well as alpha6 and beta1 integrin chains, was detected by in...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/1098-1136(20010101)33:1<36::aid-glia1004>3
更新日期:2001-01-01 00:00:00
abstract::Simvastatin, a lipophilic statin that crosses the blood-brain barrier, is being evaluated as a potential therapy for multiple sclerosis (MS) due to its anti-inflammatory properties. We assessed the effects of simvastatin on cultures of rat newborn and human fetal oligodendrocyte progenitor cells (OPCs) and human adult...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20441
更新日期:2007-01-15 00:00:00
abstract::The purpose of the present study was to elucidate the expression and regulation of the L-cystine transporter, system x(c) (-), in Müller cells. In this study, newly developed conditionally immortalized rat Müller cell lines (TR-MUL) from transgenic rats harboring the temperature-sensitive SV 40 large T-antigen gene we...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10253
更新日期:2003-09-01 00:00:00
abstract::Adult Sprague-Dawley rats were given a single dose of trimethyltin chloride (TMT). Three days following treatment, a neuronal alteration was observed in the CA3c pyramidal cell layer of hippocampus which was not accompanied by any apparent astrocyte reaction. At 1 as well as 2 weeks after treatment, a gliosis in hippo...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440110410
更新日期:1994-08-01 00:00:00
abstract::Oligodendrocyte differentiation and myelination involve dramatic changes in cell signaling pathways, gene expression patterns, cell shape, and cytoskeletal organization. In a pilot study investigating CNS angiogenesis, oligodendrocytes were intensely labeled by antisera directed against the C-terminal of Tie-2, a 140-...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20175
更新日期:2005-05-01 00:00:00
abstract::Neurons from embryonic (E18) rat hippocampus were chosen to identify and characterize neurite growth-stimulating proteins accumulating in serum-free conditioned media (CM) obtained from primary or secondary cultures of cerebral astrocytes (less than 5% nonglial cells) using a quantitative cell culture bioassay. CM wer...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440020307
更新日期:1989-01-01 00:00:00
abstract::To study the function of astrocytes in the adult brain, we have targeted the expression of E. coli nitroreductase (NTR) to the astrocytes of transgenic mice under the control of the GFAP promoter. The astrocytes expressing NTR were selectively ablated after administration of the prodrug CB1954, resulting in motor disc...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.1061
更新日期:2001-06-01 00:00:00
abstract::We characterized morphological effects of the endogenous excitotoxin, glutamate in ex vivo retinal segments prepared from 30-day-old rats. Initial changes induced by glutamate consisted of reversible, sodium-dependent Müller cell swelling. This glial swelling was mimicked by glutamate transport substrates but not by i...
journal_title:Glia
pub_type: 杂志文章
doi:
更新日期:1999-02-15 00:00:00
abstract::Reactive astrocytes are a common feature of various pathological conditions within the CNS. Morphological changes of reactive astrocytes include an altered nucleus-cytoplasm relationship, nuclear indentations, an increased amount of intermediate filaments, and an immunologically immature phenotype. Additionally, the n...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440050404
更新日期:1992-01-01 00:00:00
abstract::Minocycline, a derivative of the antibiotic tetracycline, displays neuroprotective properties in various models of neurodegenerative diseases and is now used in clinical trials, because of its relative safety and tolerability. Minocycline passes the blood-brain barrier and is presumed to inhibit microglial activation....
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20268
更新日期:2006-02-01 00:00:00
abstract::Type I interferons (IFNs), IFN-α and IFN-β, represent the major effector cytokines of the host immune response against viruses and other intracellular pathogens. These cytokines are produced via activation of numerous pattern recognition receptors, including the Toll-like receptor signaling network, retinoic acid-indu...
journal_title:Glia
pub_type: 杂志文章,评审
doi:10.1002/glia.23154
更新日期:2017-09-01 00:00:00
abstract::Gamma-secretase is an aspartyl protease composed of four proteins: presenilin (PS), nicastrin (Nct), APH1, and PEN2. These proteins assemble into a membrane complex that cleaves a variety of substrates within the transmembrane domain. The gamma-secretase cleavage products play an important role in various biological p...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20638
更新日期:2008-04-01 00:00:00
abstract::Neuropsin and protease M/neurosin are serine proteases expressed by neurons and glial cells, and serve a variety of functions in the central nervous system (CNS). The current study demonstrates changes in the expression of these proteases following hemisection of the mouse spinal cord. Within unlesioned spinal cord, n...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20058
更新日期:2004-11-01 00:00:00
abstract::Oligodendrocytes were studied in the anterior medullary velum (AMV) of the rat using the monoclonal antibody Rip, an oligodendrocyte marker of unknown function. Confocal microscopic imaging of double immunofluorescent labelling with antibodies to Rip and carbonic anhydrase II (CAII) revealed two biochemically and morp...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440140304
更新日期:1995-07-01 00:00:00
abstract::A growth cone-enriched fraction was prepared from 3-4 day rat cerebra. Examination of the growth cone fraction by electron microscopy revealed numerous structures circular in appearance that contain a number of features common to neuronal growth cones in vivo. The isolated growth cones stimulated a dose-dependent inco...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440050204
更新日期:1992-01-01 00:00:00
abstract::The p75 neurotrophin receptor has been implicated in the regulation of multiple cellular functions that differ depending on the cell context. We have observed that p75(NTR) is strongly induced on astrocytes as well as neurons in the hippocampal CA3 region after seizures; however, the function of this receptor on these...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20857
更新日期:2009-10-01 00:00:00
abstract::Forkhead transcription factor 3 (Foxp3) is critical for generating CD4(+)CD25(+) regulatory T cells. However, its role in microglia has not been identified. Here, we show that Foxp3 is expressed in microglia and is upregulated upon activation. In Foxp3 mutant mice (Foxp3(sf)), microglia release higher levels of inflam...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.21006
更新日期:2010-08-01 00:00:00
abstract::The poor regenerative ability of neurons of the central nervous system in mammals, as compared with their counterpart in fish or amphibians, is thought to stem from differences in their immediate nonneuronal environment and its response to axonal injury. We describe one aspect of the environmental response to axonal i...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440100104
更新日期:1994-01-01 00:00:00
abstract::Activation of microglia resulting in exacerbated inflammation expression plays an important role in degeneration of dopaminergic (DA) neurons in the pathogenesis of Parkinson's disease (PD). However, how this enhanced inflammation is induced in microglia remains largely unclear. Here, in the mouse PD model induced by ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23153
更新日期:2017-08-01 00:00:00
abstract::The predominant glial cells of the drone retina (outer pigment cells) respond to an increase in extracellular [K+] (Ko) by a net uptake of K+; thus, they contribute to bringing Ko back toward its baseline value. The authors report herein that there is also a different mechanism by which light stimulation of the retina...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440020402
更新日期:1989-01-01 00:00:00
abstract::Glial cell-mediated potassium and glutamate homeostases play important roles in the regulation of neuronal excitability. Diminished potassium and glutamate buffering capabilities of astrocytes result in hyperexcitability of neurons and abnormal synaptic transmission. The role of the different K+ channels in maintainin...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20455
更新日期:2007-02-01 00:00:00
abstract::The substantia gelatinosa (SG) of the spinal cord processes incoming painful information to ascending projection neurons. Whole-cell patch clamp recordings from SG spinal cord slices documented that in a low Ca(2+) /no Mg(2+) (low X(2+) ) external medium adenosine triphosphate (ATP)/dibenzoyl-ATP, Bz-ATP) caused inwar...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22707
更新日期:2014-10-01 00:00:00
abstract::Microglia contribute to pathophysiology at all stages of multiple sclerosis. Colony-stimulating factor-1 (CSF1) is crucial for microglial proliferation and activation. In this study we measured the CSF1 levels and studied its cellular expression in the mouse spinal cords with experimental autoimmune encephalomyelitis ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23464
更新日期:2018-10-01 00:00:00
abstract::When the brain suffers injury, microglia migrate to the damaged sites and become activated. These activated microglia are not detected several days later and the mechanisms underlying their disappearance are not well characterized. In this study, we demonstrate that interleukin (IL)-13, an anti-inflammatory cytokine, ...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.10057
更新日期:2002-06-01 00:00:00
abstract::Insulin-like growth factor II (IGFII) acts as a potent mitogen for several tumor types and has been reported to positively influence astrocytoma cell growth and motility. In the central nervous system, IGFII bioavailability is mainly modulated by insulin-like growth factor binding protein 2 (IGFBP2), which sequestrate...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.20719
更新日期:2008-11-15 00:00:00
abstract::Apart from dedicated oligodendroglial progenitor cells, adult neural stem cells (aNSCs) can also give rise to new oligodendrocytes in the adult central nervous system (CNS). This process mainly confers myelinating glial cell replacement in pathological situations and can hence contribute to glial heterogeneity. Our pr...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.23724
更新日期:2020-02-01 00:00:00
abstract::Segments from adult fish and rat retinae were explanted on myelin-marker expressing oligodendrocytes derived from the regenerating goldfish optic nerve. Fish axons grew in high density and even rat retinal axons regenerated to considerable length on the surface of the fish oligodendrocytes, suggesting that this type o...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.440080102
更新日期:1993-05-01 00:00:00
abstract::Multiple sclerosis (MS) frequently starts near the lateral ventricles, which are lined by subventricular zone (SVZ) progenitor cells that can migrate to lesions and contribute to repair. Because MS-induced inflammation may decrease SVZ proliferation and thus limit repair, we studied the role of galectin-3 (Gal-3), a p...
journal_title:Glia
pub_type: 杂志文章
doi:10.1002/glia.22906
更新日期:2016-01-01 00:00:00