Platelet-activating factor induces cell death in cultured astrocytes and oligodendrocytes: involvement of caspase-3.

Abstract:

:The biologically active lipid metabolite, platelet-activating factor (PAF), is thought to contribute to inflammatory processes and tissue damage in a variety of central nervous system (CNS) injuries. In previous studies, we found that after contusion spinal cord injury, treatment with a PAF antagonist led to significantly increased white matter tissue sparing as well as decreased mRNA levels for pro-inflammatory cytokines. Some studies suggest that PAF can also have toxic effects on neurons in vitro. Few studies, however, have examined the effects of PAF on glial cells of the CNS. In the present study, the potential for PAF to act as a toxin to cultured astrocytes was examined. Also investigated were the effects of PAF on oligodendrocytes at two different stages of development. Treatment with 0.02-2 microM PAF for 72 h resulted in significant levels of cell death in both cell types (P < 0.05), an effect that was blocked by the PAF receptor antagonists, WEB 2170 and BN 52021. To investigate PAF-induced glial cell death further, we looked for activation of the enzyme, caspase-3, which can be indicative of apoptosis. Immunocytochemistry demonstrated that PAF at all concentrations caused activation of caspase-3 at 24, 48, and 72 h after treatment in both cell types. Caspase-3-dependent cell death was further confirmed using knockout mice (-/-) deficient in the caspase-3 gene. Toxicity was lost when astrocytes (-/-) were exposed to 0.02-2 microM PAF (P < 0.01). Oligodendrocytes (-/-) were not susceptible to toxicity at 2 microM PAF (P < 0.001). The results demonstrate that the pro-inflammatory molecule, PAF, induces cell death in cultured CNS glial cells and that this effect is, in part, dependent on caspase-3 activation.

journal_name

Glia

journal_title

Glia

authors

Hostettler ME,Knapp PE,Carlson SL

doi

10.1002/glia.10065

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

228-39

issue

3

eissn

0894-1491

issn

1098-1136

journal_volume

38

pub_type

杂志文章

相关文献

GLIA文献大全
  • ATP-induced chemotaxis of microglial processes requires P2Y receptor-activated initiation of outward potassium currents.

    abstract::Microglial cells are the resident macrophages that are involved in brain injuries and infections. Recent studies using transcranial two-photon microscopy have shown that ATP and P2Y receptors mediated rapid chemotactic responses of miroglia to local injury. However, the molecular mechanism for microglial chemotaxis to...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20500

    authors: Wu LJ,Vadakkan KI,Zhuo M

    更新日期:2007-06-01 00:00:00

  • Sequence of oligodendrocyte development in the human fetal telencephalon.

    abstract::Oligodendrocytes (OL), cells that myelinate axons in the CNS, differentiate from early to late oligodendrocyte progenitor cells (OPC) to become mature OL. Unlike the case in the rodent brain, myelin formation starts prenatally in the human brain, but the sequence of OL development and the onset of myelination are not ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20134

    authors: Jakovcevski I,Zecevic N

    更新日期:2005-03-01 00:00:00

  • Identification of Golli and myelin basic proteins in human brain during early development.

    abstract::The myelin basic protein gene (Mbp) encodes for the major myelin structural proteins and it is included in the Golli-Mbp gene complex. Previously, we observed MBP-like proteins in the human central nervous system (CNS) at developmental stages preceding myelination. In an effort to distinguish between Golli (HOG5 and H...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10028

    authors: Tosic M,Rakic S,Matthieu J-,Zecevic N

    更新日期:2002-03-01 00:00:00

  • Structural organization of the perivascular astrocyte endfeet and their relationship with the endothelial glucose transporter: a confocal microscopy study.

    abstract::Despite the increasing evidence for a prominent role played by the perivascular endfeet of astrocytes in the functional metabolic coupling between astrocytes and neurons, a clear picture of their spatial organization is still lacking. To examine the three-dimensional structure of the astrocyte endfeet and their relati...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Kacem K,Lacombe P,Seylaz J,Bonvento G

    更新日期:1998-05-01 00:00:00

  • Neurofibromatosis 1 (NF1) heterozygosity results in a cell-autonomous growth advantage for astrocytes.

    abstract::Individuals with neurofibromatosis 1 (NF1) develop low-grade astrocytomas at an increased frequency. To gain insight into the function of the Nf1 gene product as a growth regulator for astrocytes, we examined mice heterozygous for a targeted Nf1 mutation. In our previous studies, we demonstrated increased numbers of p...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/1098-1136(20010315)33:4<314::aid-glia1030>

    authors: Bajenaru ML,Donahoe J,Corral T,Reilly KM,Brophy S,Pellicer A,Gutmann DH

    更新日期:2001-03-15 00:00:00

  • Remote astrocytic response as demonstrated by glial fibrillary acidic protein immunohistochemistry in the visual cortex of dorsal lateral geniculate nucleus lesioned rats.

    abstract::The reaction of astroglia was investigated after unilateral destruction of the dorsal lateral geniculate nucleus in the primary visual cortex of adult albino rats. The destruction of the dorsal lateral geniculate nucleus was performed by stereotaxic injections of ibotenic acid, and the location was verified in Nissl s...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440030410

    authors: Hajós F,Kálmán M,Zilles K,Schleicher A,Sótonyi P

    更新日期:1990-01-01 00:00:00

  • Terminal complement complexes concomitantly stimulate proliferation and rescue of Schwann cells from apoptosis.

    abstract::The consequences of sublytic terminal complement complex (TCC) assembly on Schwann cell proliferation and apoptosis were examined by using purified complement proteins (C5*-9) or antibody-sensitized Schwann cells in the presence of a serum that was depleted of the seventh component of complement (C7dHS) and reconstitu...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(200004)30:2<187::aid-glia8

    authors: Dashiell SM,Rus H,Koski CL

    更新日期:2000-04-01 00:00:00

  • Neuronal hyperactivity induces astrocytic expression of neurocan in the adult rat hippocampus.

    abstract::Extracellular matrix molecules are involved in the cellular functions of proliferation, migration, morphological differentiation, and synaptic plasticity. One candidate molecule of the extracellular matrix is the chondroitin sulfate proteoglycan neurocan. To determine whether neurocan expression is regulated by neuron...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20329

    authors: Schwarzacher SW,Vuksic M,Haas CA,Burbach GJ,Sloviter RS,Deller T

    更新日期:2006-05-01 00:00:00

  • Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery.

    abstract::Schwann cells (SCs) and olfactory ensheathing glia (OEG) have shown promise for spinal cord injury repair. We sought their in vivo identification following transplantation into the contused adult rat spinal cord at 1 week post-injury by: (i) DNA in situ hybridization (ISH) with a Y-chromosome specific probe to identif...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20490

    authors: Pearse DD,Sanchez AR,Pereira FC,Andrade CM,Puzis R,Pressman Y,Golden K,Kitay BM,Blits B,Wood PM,Bunge MB

    更新日期:2007-07-01 00:00:00

  • Interactions between TLR7 and TLR9 agonists and receptors regulate innate immune responses by astrocytes and microglia.

    abstract::Toll-like receptors 7 (TLR7) and 9 (TLR9) are important mediators of innate immune responses. Both receptors are located in endosomal compartments, recognize nucleic acids, and signal via Myeloid differentiation factor 88 (MyD88). In the current study, we analyzed TLR7 and TLR9 induced activation of astrocytes and mic...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20952

    authors: Butchi NB,Du M,Peterson KE

    更新日期:2010-04-15 00:00:00

  • ErbB signaling has a role in radial sorting independent of Schwann cell number.

    abstract::In the peripheral nervous system, Schwann cells make myelin, a specialized sheath that is essential for rapid axonal conduction of action potentials. Immature Schwann cells initially interact with many axons, but, through a process termed radial sorting, eventually interact with one segment of a single axon as promyel...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21175

    authors: Raphael AR,Lyons DA,Talbot WS

    更新日期:2011-07-01 00:00:00

  • T- and B-cell responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis and multiple sclerosis.

    abstract::The identification of myelin oligodendrocyte glycoprotein (MOG) as a target for autoantibody-mediated demyelination in experimental autoimmune encephalomyelitis (EAE) resulted in the re-evaluation of the role of B cell responses to myelin autoantigens in the immunopathogenesis of multiple sclerosis. MOG is a central n...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.1111

    authors: Iglesias A,Bauer J,Litzenburger T,Schubart A,Linington C

    更新日期:2001-11-01 00:00:00

  • Cathepsin S release from primary cultured microglia is regulated by the P2X7 receptor.

    abstract::Microglia respond rapidly to injury, increasing their synthesis and release of inflammatory mediators, many of which contribute to the maintenance of persistent pain following CNS or PNS injury. We have recently shown that the lysosomal cysteine protease Cathepsin S (CatS) expressed by spinal microglia is vital for th...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21042

    authors: Clark AK,Wodarski R,Guida F,Sasso O,Malcangio M

    更新日期:2010-11-01 00:00:00

  • Functional characterization of mannose receptor expressed by immunocompetent mouse microglia.

    abstract::The mannose receptor is a pattern-recognition receptor involved in innate and adaptive immunity. The receptor is mainly expressed by macrophages and, within the brain, by astrocytes and microglia. This study reports for the first time the effects of two classical proinflammatory (interferon-gamma, IFNgamma) and anti-i...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10196

    authors: Zimmer H,Riese S,Régnier-Vigouroux A

    更新日期:2003-04-01 00:00:00

  • Decreased estradiol release from astrocytes contributes to the neurodegeneration in a mouse model of Niemann-Pick disease type C.

    abstract::Niemann-Pick disease type C (NPC) is a deadly neurodegenerative disease often caused by mutation in a gene called NPC1, which results in the accumulation of unesterified cholesterol and glycosphingolipids in the endosomal-lysosomal system. Most studies on the mechanisms of neurodegeneration in NPC have focused on neur...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20563

    authors: Chen G,Li HM,Chen YR,Gu XS,Duan S

    更新日期:2007-11-15 00:00:00

  • Astrocytes protect neurons from neurotoxic injury by serum glutamate.

    abstract::Serum is used widely for culturing neurons and glial cells, and is thought to provide essential, albeit undefined, factors such as hormones, growth factors, and trace elements that promote the growth of cells in vitro. Moreover, serum can have profound effects on cell proliferation, differentiation, and cell morpholog...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199803)22:3<237::aid-glia3

    authors: Ye ZC,Sontheimer H

    更新日期:1998-03-01 00:00:00

  • Expression of alpha vbeta3 and alpha vbeta8 integrins during oligodendrocyte precursor differentiation in the presence and absence of axons.

    abstract::We have shown previously that switching of the alpha v-associated beta1 and beta5 integrin subunits during differentiation of myelin-forming oligodendrocytes may regulate important aspects of cell behaviour such as migration (Milner et al., 1996: J Neurosci 16:7240-7252). In this study we have examined the development...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Milner R,Frost E,Nishimura S,Delcommenne M,Streuli C,Pytela R,Ffrench-Constant C

    更新日期:1997-12-01 00:00:00

  • Na+ channels of Müller (glial) cells isolated from retinae of various mammalian species including man.

    abstract::Within the last few years, the expression of voltage-dependent, TTX-sensitive Na+ channels has been demonstrated in several types of neuroglial cells such as astrocytes and Schwann cells. Recently, we reported the occurrence of such Na+ currents in retinal Müller (glial) cells from dog and cat. This paper deals with t...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440100304

    authors: Chao TI,Skachkov SN,Eberhardt W,Reichenbach A

    更新日期:1994-03-01 00:00:00

  • Aquaporin-4-independent volume dynamics of astroglial endfeet during cortical spreading depression.

    abstract::Cortical spreading depression (CSD) is a slowly propagating wave of depolarization of gray matter. This phenomenon is believed to underlie the migraine aura and similar waves of depolarization may exacerbate injury in a number of neurological disease states. CSD is characterized by massive ion dyshomeostasis, cell swe...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23604

    authors: Rosic B,Dukefoss DB,Åbjørsbråten KS,Tang W,Jensen V,Ottersen OP,Enger R,Nagelhus EA

    更新日期:2019-06-01 00:00:00

  • Activation of dorsal horn microglia contributes to diabetes-induced tactile allodynia via extracellular signal-regulated protein kinase signaling.

    abstract::Painful neuropathy is one of the most common complications of diabetes, one hallmark of which is tactile allodynia (pain hypersensitivity to innocuous stimulation). The underlying mechanisms of tactile allodynia are, however, poorly understood. Emerging evidence indicates that, following nerve injury, activated microg...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20623

    authors: Tsuda M,Ueno H,Kataoka A,Tozaki-Saitoh H,Inoue K

    更新日期:2008-03-01 00:00:00

  • Ultrastructural relationships between noradrenergic nerve fibers and non-neuronal elements in the rat cerebral cortex.

    abstract::Pharmacological and biochemical data suggest that noradrenaline (NA)-containing fibers not only regulate the activity of cortical neurons but also influence the functional state of non-neuronal elements. In the present study, immunocytochemistry with an antiserum against NA, followed by silver-gold intensification of ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-1136(199606)17:2<133::AID-GLIA5

    authors: Paspalas CD,Papadopoulos GC

    更新日期:1996-06-01 00:00:00

  • Association between cytoplasmic CRABP2, altered retinoic acid signaling, and poor prognosis in glioblastoma.

    abstract::Retinoic acid (RA), a metabolite of vitamin A, is required for the regulation of growth and development. Aberrant expression of molecules involved in RA signaling has been reported in various cancer types including glioblastoma multiforme (GBM). Cellular retinoic acid-binding protein 2 (CRABP2) has previously been sho...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22976

    authors: Liu RZ,Li S,Garcia E,Glubrecht DD,Poon HY,Easaw JC,Godbout R

    更新日期:2016-06-01 00:00:00

  • Ca2+ influx into leech neuropile glial cells mediated by nicotinic acetylcholine receptors.

    abstract::The effect of cholinergic agonists and antagonists on the intracellular free Ca2+ concentration ([Ca2+]i) of leech neuropile glial cells was investigated by use of iontophoretically injected fura-2. In neuropile glial cells, cholinergic agonists induced a marked increase in [Ca2+]i that was inhibited by d-tubocurarine...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440150106

    authors: Hochstrate P,Schlue WR

    更新日期:1995-09-01 00:00:00

  • Epidermal growth factor receptor in proliferating reactive glia following transient focal ischemia in the rat brain.

    abstract::Severe transient focal cerebral ischemia causes brain infarction with a strong glial reaction. We have studied whether postischemic reactive glial cells express epidermal growth factor receptor (EGFR) following middle cerebral artery occlusion in the rat. We have also looked for signs of proliferating activity, as EGF...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199806)23:2<120::aid-glia3

    authors: Planas AM,Justicia C,Soriano MA,Ferrer I

    更新日期:1998-06-01 00:00:00

  • Carbon monoxide controls microglial erythrophagocytosis by regulating CD36 surface expression to reduce the severity of hemorrhagic injury.

    abstract::Microglial erythrophagocytosis is crucial in injury response to hemorrhagic stroke. We hypothesized that regulation of microglial erythrophagocytosis via HO-1/CO depends on a pathway involving reactive oxygen species (ROS) and CD36 surface-expression. The microglial BV-2 cell line and primary microglia (PMG) were incu...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23864

    authors: Kaiser S,Selzner L,Weber J,Schallner N

    更新日期:2020-11-01 00:00:00

  • Potentiation of cannabinoid signaling in microglia by adenosine A2A receptor antagonists.

    abstract::Neuroprotective M2-skewed microglia appear as promising to alter the course of neurodegenerative diseases and G protein-coupled receptors (GPCRs) are potential targets to achieve such microglial polarization. A common feature of adenosine A2A (A2A R) and cannabinoid CB2 (CB2 R) GPCRs in microglia is that their express...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23694

    authors: Franco R,Reyes-Resina I,Aguinaga D,Lillo A,Jiménez J,Raïch I,Borroto-Escuela DO,Ferreiro-Vera C,Canela EI,Sánchez de Medina V,Del Ser-Badia A,Fuxe K,Saura CA,Navarro G

    更新日期:2019-12-01 00:00:00

  • Cell death and neuronal differentiation of glioblastoma stem-like cells induced by neurogenic transcription factors.

    abstract::Glioblastoma multiform (GBM) are devastating brain tumors containing a fraction of multipotent stem-like cells which are highly tumorigenic. These cells are resistant to treatments and are likely to be responsible for tumor recurrence. One approach to eliminate GBM stem-like cells would be to force their terminal diff...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22429

    authors: Guichet PO,Bieche I,Teigell M,Serguera C,Rothhut B,Rigau V,Scamps F,Ripoll C,Vacher S,Taviaux S,Chevassus H,Duffau H,Mallet J,Susini A,Joubert D,Bauchet L,Hugnot JP

    更新日期:2013-02-01 00:00:00

  • Rehabilitative therapies differentially alter proliferation and survival of glial cell populations in the perilesional zone of cortical infarcts.

    abstract::Rehabilitative therapies after stroke are designed to improve remodeling of neuronal circuits and to promote functional recovery. Only very little is known about the underlying cellular mechanisms. In particular, the effects of rehabilitative training on glial cells, which play an important role in the pathophysiology...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20632

    authors: Keiner S,Wurm F,Kunze A,Witte OW,Redecker C

    更新日期:2008-04-01 00:00:00

  • Increase of MCP-1 (CCL2) in myelin mutant Schwann cells is mediated by MEK-ERK signaling pathway.

    abstract::Macrophages are critically involved in the pathogenesis of genetically caused demyelination, as it occurs in inherited demyelinating neuropathies. On the basis of the observation that upregulation of the Schwann cell-derived chemokine MCP-1 (CCL2) is a pathologically relevant mechanism for macrophage activation in mic...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20657

    authors: Fischer S,Weishaupt A,Troppmair J,Martini R

    更新日期:2008-06-01 00:00:00

  • The function of RhoGTPases in axon ensheathment and myelination.

    abstract::RhoGTPases are molecular switches that integrate extracellular signals to perform diverse cellular responses. This ability relies on the network of proteins regulating RhoGTPases activity and localization, and on the interaction of RhoGTPases with many different cellular effectors. Myelination is an ideal place for Rh...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.20752

    authors: Feltri ML,Suter U,Relvas JB

    更新日期:2008-11-01 00:00:00