Abstract:
:In order to study the role of interleukin-1beta (IL-1beta) in homeostasis, hypoxia and recovery of neuronal cells, we studied the expression and release of tumor necrosis factor-alpha (TNF-alpha) and nerve growth factor (NGF), in relation to the presence or absence of this cytokine in culture medium. Moreover, we evaluated cell mortality in the same conditions. For this aim, we used untreated and IL-1beta pre-immunoneutralized hippocampal neuronal cultures exposed to mild hypoxic stress and left to reoxygenate. Semiquantitative reverse-transciptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) determined gene expression and protein levels. Mild hypoxic stress provokes a decrease in both the expression and release of TNF-alpha and NGF. IL-1beta neutralization results in an inversion of this pattern since treated hypoxic cultures exhibited an increase of both expression and release of NGF. In pretreated hypoxic cells the increased expression of TNF-alpha was not followed by a rise in release. Reoxygenation reversed the observed effects in both cultures and the levels of cytokine expression and release were approaching control values. Our data show that in physiological conditions IL-1beta may have a neuroprotective action through positive modulation of NGF. Contrary to that, in presence of insult, IL-1beta may have an opposite role, since neutralization provoked an increase of expression and release of NGF. In addition, we demonstrated that neuronal cells are biochemically capable, not only of maintaining and recovering the homeostasis, but also of activating the appropriate response to insult. IL-1beta may have a pivotal role in this mechanism through the modulation of NGF and to a lesser degree of TNF-alpha.
journal_name
J Neuroimmunoljournal_title
Journal of neuroimmunologyauthors
Di Loreto S,Corvetti L,Maccarone R,Piancatelli D,Adorno Ddoi
10.1016/s0165-5728(00)00209-5subject
Has Abstractpub_date
2000-07-01 00:00:00pages
32-42issue
1-2eissn
0165-5728issn
1872-8421pii
S0165572800002095journal_volume
106pub_type
杂志文章abstract::Carbon nantotubes (CNTs) are emerging as a new family of nanovectors for drug and gene delivery into biological systems. To evaluate potential application of this technology for brain tumor therapy, we studied uptake and toxicity of multi-walled CNTs (MWCNTs) in the GL261 murine intracranial glioma model. Within 24 h ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2008.12.006
更新日期:2009-03-31 00:00:00
abstract::Resident glial cells and invading inflammatory cells are responsible for cytokine production within the brain. Astrocytes are known to secrete a variety of cytokines upon stimulation with cytokines themselves, protein kinase C activators, bacterial or viral constituents. Astrocytes also have surface receptors for a wi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(93)90286-8
更新日期:1993-08-01 00:00:00
abstract::Nogo-A is a protein associated with central nervous system (CNS) myelin thought to impair regenerative responses and to suppress sprouting and plastic changes of synaptic terminals. In this study, we report that serum IgM autoantibodies to the recombinant large N-terminal inhibitory domain of Nogo-A are a frequent fin...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2003.09.010
更新日期:2003-12-01 00:00:00
abstract::An electroimmunoblotting technique was used with a monoclonal antibody to myelin basic protein (MBP) to assess demyelination in 3 defined regions of the spinal cord in rats with acute experimental autoimmune encephalomyelitis (EAE). A slight loss in MBP was detected only in the sacrococcygeal region of the spinal cord...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(85)80035-7
更新日期:1985-10-01 00:00:00
abstract::Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic inflammatory myelopathy. The pathophysiology of HAM/TSP is not yet fully understood; therefore, effective therapy remains a challenging issue. This study was designed to evaluate the efficacy of interfe...
journal_title:Journal of neuroimmunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.jneuroim.2012.05.004
更新日期:2012-09-15 00:00:00
abstract::We evaluated the association of the platelet-activating factor receptor (PAFR) gene polymorphism (A224D) with the susceptibility and severity of multiple sclerosis (MS) in a Japanese population. DNA was collected from 162 Japanese patients with clinically definite 'conventional' MS (MS) and 245 healthy controls. The m...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2004.12.014
更新日期:2005-04-01 00:00:00
abstract::Previous work in our laboratory has shown that cytokine production by primary murine macrophages, and macrophage cell lines, is inhibited following treatment with the kappa-opioid agonist U50,488H. Furthermore, we have found that the participation of both accessory cells and T cells in an antibody response is suppress...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00018-0
更新日期:1997-05-01 00:00:00
abstract::HLA-DR expression on circulating monocytes varies as a function of disease activity in patients with multiple sclerosis (MS), a putative immunopathological demyelinating disorder. Specifically, monocytes isolated from subjects with active MS exhibit reduced HLA-DR antigen density, and immunoregulatory aberrations such...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(92)90001-2
更新日期:1992-04-01 00:00:00
abstract::The Lyme disease agent Borrelia garinii has been suggested to be neurotrop, preferentially affecting the nervous system. We compared the secretion of interferon-gamma in response to outer surface proteins from Borrelia garinii and Borrelia afzelii in 10 patients with neuroborreliosis. In cerebrospinal fluid, stimulati...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(98)00136-2
更新日期:1998-08-14 00:00:00
abstract::Muscle weakness in MuSK myasthenia gravis (MG) is caused predominantly by IgG4 antibodies which block MuSK signalling and destabilize neuromuscular junctions. We determined whether the binding pattern of MuSK IgG4 antibodies change throughout the disease course ("epitope spreading"), and affect disease severity or tre...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2015.12.016
更新日期:2016-02-15 00:00:00
abstract::In this report, the role of STAT4 and local production of interleukin (IL)-12 in the central nervous system (CNS) were examined during experimental vesicular stomatitis virus (VSV) encephalitis. We have previously shown that IL-12 treatment is beneficial both in vitro and in vivo during experimental VSV infection. Thi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(02)00257-6
更新日期:2002-10-01 00:00:00
abstract::Astrocytes provide crucial support for neurons and their impairment by viruses or their interactions with anti-viral or autoimmune responses could contribute to neurological disease. We have developed a transgenic mouse model to assess lymphocyte-astrocyte interactions. The major histocompatibility complex (MHC) class...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(94)90163-5
更新日期:1994-06-01 00:00:00
abstract::Multiple sclerosis (MS) is a demyelinating disease characterized by the breakdown of the blood-brain barrier (BBB), and accumulation of inflammatory infiltrates in the central nervous system. Tight junctions are specialized cell-cell adhesion structures and critical components of the BBB that have previously been show...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2010.08.011
更新日期:2010-12-15 00:00:00
abstract::Depression and chronic fatigue are frequently present in multiple sclerosis (MS); however, the prevalence rates have not been investigated in neuromyelitis optica (NMO). Thirty-nine consecutive NMO and 75 MS patients were compared using self-rating questionnaires for depressive states, daily activity, and fatigue, as ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2015.05.007
更新日期:2015-06-15 00:00:00
abstract::Tumor necrosis factor-alpha (TNF-alpha) has well recognized effects on cerebral endothelial cells and, therefore, may mediate disruption of blood-brain barrier in patients with multiple sclerosis (MS). To evaluate the in vivo relationship of TNF-alpha to blood-brain barrier impairment in MS, levels of this cytokine in...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(92)90087-2
更新日期:1992-05-01 00:00:00
abstract::The two D1-like dopamine receptor subtypes, D1a and D1b, are structurally similar and pharmacologically indistinguishable using currently available ligands. To differentiate between the D1-like dopamine receptor subtypes, murine monoclonal antibodies to the rat Dla and the rat D1b dopamine receptor have been prepared....
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(99)00142-3
更新日期:1999-11-15 00:00:00
abstract::Immunofluorescence, cell binding assays and enzyme immunoassays were used to investigate the expression of class II major histocompatibility antigens on peripheral blood monocytes in 67 patients with multiple sclerosis. Monocytes from patients with active disease expressed fewer HLA-DR molecules on their surface than ...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(89)90015-5
更新日期:1989-05-01 00:00:00
abstract::The capacity of three different human glioblastoma cell lines to activate human T cells was analysed by measuring major histocompatibility complex (MHC) antigen expression, monokine secretion and lectin, mAb OKT3 and antigen-driven T cell proliferation. All glioblastoma cells tested were able to induce PHA and concana...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/0165-5728(92)90191-m
更新日期:1992-11-01 00:00:00
abstract::In the present study, we investigated the B-cell translocation gene 1 (BTG1) as a candidate for multiple sclerosis (MS) susceptibility. BTG1 is a member of a family of genes involved in the apoptotic process. We genotyped two SNPs of the BTG1 gene (rs731652 and rs12694) in 550 MS patients and 548 controls. For SNP rs7...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2009.05.010
更新日期:2009-08-18 00:00:00
abstract::Activation of microglia cells play critical role in neuroinflammation after brain injury. Exosomes from adipose-derived stem cells (ADSC) possess immunoregulation effect similar with ADSC. We hypothesized that ADSC derived exosomes (ADSC-exosomes) could inhibit the activation of microglia cells and prevent neuroinflam...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2019.576996
更新日期:2019-09-15 00:00:00
abstract::In the century and a half since multiple sclerosis (MS) was first recognized, the pathology of the condition has been defined with increasing detail. From the recognition and definition of MS as a clinical phenomenon, studies of the diseased brain tissue have progressed in a manner dependent on the science of the time...
journal_title:Journal of neuroimmunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/s0165-5728(99)00079-x
更新日期:1999-07-01 00:00:00
abstract::IFN-gamma production is a hallmark of Th1 response, and IFN-gamma has multiple roles in Th1 development, depending on the experimental conditions. In this study, the correlation between IFN-gamma and interleukin 12 receptor beta2 (IL-12R beta 2) expression was investigated in experimental autoimmune uveitis (EAU) susc...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(03)00071-7
更新日期:2003-04-01 00:00:00
abstract::We here evaluated the central dopaminergic response in sepsis survivor rats using the administration of D-amphetamine. Male Wistar rats underwent cecal ligation and perforation (CLP) or were sham-operated. After 10 days of recovery, rats received an intraperitoneal injection of D-amphetamine 0.5, 1 and 2 mg/kg or sali...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2009.04.005
更新日期:2009-07-25 00:00:00
abstract::The protein tyrosine phosphatase, SHP-1, is a negative regulator of proinflammatory signaling and autoimmune disease. We have previously reported reduced SHP-1 expression in peripheral blood leukocytes of subjects with multiple sclerosis (MS). Recent evidence indicates that virus-induced DNA methylation of the SHP-1 p...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2012.03.003
更新日期:2012-05-15 00:00:00
abstract::Plasma B cells secrete immunoglobulinfree light chains (IgLC) which by binding to mast cells can mediate hypersensitivity responses and are involved in several immunological disorders. To investigate the effects of antigen-specific IgLC activation, intracellular recordings were made from cultured murine dorsal root ga...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.08.015
更新日期:2013-11-15 00:00:00
abstract::Myelin proteolipid protein (PLP) is a prime candidate autoantigen for multiple sclerosis. In order to define potential immunodominant epitopes, T cell lines (TCL) from the peripheral blood of HLA-DR 15(2) MS patients were established which responded to the intact molecule of PLP. These TCL were then tested in individu...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(97)00260-9
更新日期:1998-04-15 00:00:00
abstract::The BeAn strain of Theiler's murine encephalomyelitis virus (TMEV) persists in the CNS and produces a chronic inflammatory demyelinating disease that is an animal model for human multiple sclerosis (MS). The mechanisms leading to TMEV-induced demyelination are still under study but most likely involve both immune-medi...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(01)00338-1
更新日期:2001-08-30 00:00:00
abstract::CR-EAE is a Th1-mediated inflammatory autoimmune demyelinating disease of the CNS and serves as a model of human multiple sclerosis. Our previous studies have shown the protective effect of orally administered lisofylline in the prevention of active and passively induced acute EAE. In our present studies we have exami...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/s0165-5728(00)00348-9
更新日期:2000-10-02 00:00:00
abstract::Prion diseases are neurodegenerative disorders characterized by the accumulation of misfolded prion protein. In a previous study, we showed that neurotoxic prion peptide (PrP106-126) induced NALP3 inflammasome activation in mouse primary and immortalized microglia. In the present work, we examined the relevance of pha...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2013.04.016
更新日期:2013-07-15 00:00:00
abstract::The purpose of this study was to determine if alpha-tocopherol-a reactive oxygen species (ROS) scavenging agent-inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The L...
journal_title:Journal of neuroimmunology
pub_type: 杂志文章
doi:10.1016/j.jneuroim.2003.12.017
更新日期:2004-04-01 00:00:00