Abstract:
:The reliability, relation to whole-body maximal oxygen uptake (VO(2max)), and force-level sensitivity of (31)P MRS markers of mitochondrial function were studied in 39 normal-weight women. Following 90 s isometric plantar-flexion exercises at 45, 70 and 100% of maximum voluntary contraction, skeletal muscle mitochondrial function was determined from the phosphocreatine recovery time constant (TC(PCr)), the ADP recovery time constant (TC(ADP)), and the rate of change in PCr during the first 14 s of recovery (OxPhos). VO(2max) was measured on a treadmill. Test-retest measurements were obtained in a subset of seven women. Overall, TC(PCr), TC(ADP) and OxPhos were reproducible for all exercises (coefficients of variation = 2.3-19.3%). With increasing force-level, TC(PCr) was prolonged (29.0 +/- 8.2, 31.9 +/- 9.0 and 35.4 +/- 9.5 s), OxPhos was increased (0.159 +/- 0.081, 0.247 +/- 0.090 and 0.310 +/- 0.114), and TC(ADP) was shortened (22.4 +/- 7.9, 21.3 +/- 6.2, and 19.5 +/- 6.7; p < 0.01). All MRS markers of mitochondrial function were correlated with VO(2max) (r = 0.41-0.72; p < 0.05). These results suggest that measurements of TC(PCr), TC(ADP) and OxPhos yield reproducible results that correlate with whole-body VO(2max) and that vary in force-level sensitivity.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Larson-Meyer DE,Newcomer BR,Hunter GR,Hetherington HP,Weinsier RLdoi
10.1002/(sici)1099-1492(200002)13:1<14::aid-nbm605subject
Has Abstractpub_date
2000-01-01 00:00:00pages
14-27issue
1eissn
0952-3480issn
1099-1492pii
10.1002/(SICI)1099-1492(200002)13:1<14::AID-NBM605journal_volume
13pub_type
杂志文章abstract::Citicoline supplementation has been used to ameliorate memory disturbances in older people and those with Alzheimer's disease. This study used MRS to characterize the effects of citicoline on high-energy phosphate metabolites and constituents of membrane synthesis in the frontal lobe. Phosphorus ((31)P) metabolite dat...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1281
更新日期:2008-11-01 00:00:00
abstract::There has been growing interest in understanding energy metabolism in human embryos generated using assisted reproductive techniques (ART) for improving the overall success rate of the method. Using NMR spectroscopy as a noninvasive tool, we studied human embryo metabolism to identify specific biomarkers to assess the...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2814
更新日期:2013-01-01 00:00:00
abstract::Following the impetus of early clinical and experimental investigations, in vivo and in vitro MRS studies of tumours pointed in the eighties to the possible significance of signals arising from phospholipid (PL) precursors and catabolites as novel biochemical indicators of in vivo tumour progression and response to th...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/(sici)1099-1492(199911)12:7<413::aid-nbm58
更新日期:1999-11-01 00:00:00
abstract::By quantification of brain metabolites, localized brain proton MRS can non-invasively provide biochemical information from distinct regions of the brain. Quantification of short-TE signals is usually based on a metabolite basis set. The basis set can be obtained by two approaches: (1) by measuring the signals of metab...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1234
更新日期:2008-07-01 00:00:00
abstract::A study was undertaken to determine if the vascular characteristics measured by dynamic contrast-enhanced magnetic resonance imaging (primarily permeability surface area product and extracellular-extravascular tissue volume fraction) would be beneficial in explaining the inter-lesion metabolic heterogeneity displayed ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.675
更新日期:2000-12-01 00:00:00
abstract::The applications of in vivo electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy have been impressive over a relatively short period despite the many obstacles which had to be overcome, such as dielectric absorption and biodestruction. The loop-gap resonators and modified loop coil syste...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.898
更新日期:2004-08-01 00:00:00
abstract::The background underpinning the clinical use of ultrashort echo time, SPRITE and other pulse sequences for imaging bone and other connective tissues with short T2 is reviewed. Features of the basic physics relevant to UTE imaging are described, including the consequences when the radiofrequency pulse duration is of th...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1100
更新日期:2006-11-01 00:00:00
abstract::Magnetic resonance elastography (MRE) is a non-invasive imaging technique used to visualise and quantify mechanical properties of tissue, providing information beyond what can be currently achieved with standard MR sequences and could, for instance, provide new insight into pathological processes in the brain. This st...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1254
更新日期:2008-08-01 00:00:00
abstract::Increased lactate production through glycolysis in aerobic conditions is a hallmark of cancer. Some anticancer drugs have been designed to exploit elevated glycolysis in cancer cells. For example, lonidamine (LND) inhibits lactate transport, leading to intracellular acidification in cancer cells. Chemical exchange sat...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3287
更新日期:2015-05-01 00:00:00
abstract::Mouse models are increasingly used to study the pathophysiology of myocardial infarction in vivo. In this area, MRI has become the gold standard imaging modality, because it combines high spatial and temporal resolution functional imaging with a large variety of methods to generate soft tissue contrast. In addition, (...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.2767
更新日期:2012-08-01 00:00:00
abstract::During the measurement of hyperpolarized 129 Xe magnetic resonance imaging (MRI), the diffusion-weighted imaging (DWI) technique provides valuable information for the assessment of lung morphometry at the alveolar level, whereas the chemical shift saturation recovery (CSSR) technique can evaluate the gas exchange func...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3730
更新日期:2017-08-01 00:00:00
abstract::Results are presented from an in vivo study of human skin in which a zig-zag surface coil together with a three-pulse Fourier Series Window (FSW) protocol have been used to obtain minimally contaminated 31P skin spectra of the posterior calf. Phantom experiments indicate that while the fall-off in the B1 field profile...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199608)9:5<195::AID-NBM416
更新日期:1996-08-01 00:00:00
abstract::Spin-exchange NMR techniques enable the measurement of the rates of exchange of solutes between chemically or physically distinct sites in reactions taking place at chemical equilibrium. The time scale of the events that are able to be investigated lies in the neighbourhood of 1 s. The earliest studies in this area of...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940030303
更新日期:1990-06-01 00:00:00
abstract::The phosphorus NMR spectra of isolated perfused rat liver displays a prominent inorganic phosphate peak at 2.76 +/- 0.05 ppm relative to liver glycerolphosphocholine at 0.49 ppm. From titration curves of phosphorus-containing compounds this corresponds to a pH of 7.4. The spectra also display a shoulder on the promine...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940010207
更新日期:1988-04-01 00:00:00
abstract::The purpose of this study was to investigate the reliability of 3D isotropic MRI and quantitative multi-parametric MRI characterization on an orthotopic pancreatic head tumor model in rats. 3D isotropic T2 -weighted MRI was performed as a routine for tumor longitudinal follow-up and volume estimation. Common bile duct...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3676
更新日期:2017-02-01 00:00:00
abstract::γ-Aminobutyric acid (GABA) and glutamate (Glu), major neurotransmitters in the brain, are recycled through glutamine (Gln). All three metabolites can be measured by magnetic resonance spectroscopy in vivo, although GABA measurement at 3 T requires an extra editing acquisition, such as Mescher-Garwood point-resolved sp...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3847
更新日期:2018-01-01 00:00:00
abstract::The main aim of this study was to employ high-resolution MRI to investigate the spatiotemporal development of pathological features associated with contusive spinal cord injury (SCI) in mice. Experimental mice were subjected to either sham surgery or moderate contusive SCI. A 16.4-T small-animal MR system was employed...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2829
更新日期:2013-02-01 00:00:00
abstract::Hyperpolarised (HP) (13)C NMR allows enzymatic activity to be probed in real time in live biological systems. The use of in vitro models gives excellent control of the cellular environment, crucial in the understanding of enzyme kinetics. The increased conversion of pyruvate to lactate in cancer cells has been well st...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3341
更新日期:2015-08-01 00:00:00
abstract::The intimal and medial linings of the pulmonary artery consist largely of vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs), respectively. The migration of these cell types to a potential tissue-engineered pulmonary valve (TEPV) implant process is therefore of interest in understanding the val...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1642
更新日期:2012-03-01 00:00:00
abstract::Both post-contrast myocardial T1 and extracellular volume (ECV) have been reported to be associated with diffuse interstitial fibrosis. Recently, the cardiovascular magnetic resonance (CMR) field is recognizing that post-contrast myocardial T1 is sensitive to several confounders and migrating towards ECV as a measure ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3221
更新日期:2014-11-01 00:00:00
abstract::An often-employed strategy to enhance signals in (31) P MRS is the generation of the nuclear Overhauser effect (NOE) by saturation of the water resonance. However, NOE allegedly increases the variability of the (31) P data, because variation is reported in NOE enhancements. This would negate the signal-to-noise (SNR) ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3455
更新日期:2016-03-01 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is characterized at histology by steatosis, hepatocyte ballooning and inflammatory infiltrates, with or without fibrosis. Although diamagnetic material in fibrosis and inflammation can be detected with quantitative susceptibility imaging, fatty acid composition changes in NASH rela...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3766
更新日期:2017-10-01 00:00:00
abstract::Progress in the development of non-proton NMR applications in medical research has been hampered by the generally low sensitivity of these nuclides as well as the lack of instrumentation readily available for studying these spins. For example, custom head coils suitable for sodium imaging of the human head are either ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940020308
更新日期:1989-09-01 00:00:00
abstract::The application of 31P MR spectroscopy in the characterization and treatment of malignant human extremity tumors is reviewed and placed in the perspective of results obtained in murine sarcomas. Despite the now widespread acquisition of gradient localized spectral maps, the low spatial resolution that can be achieved ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/(sici)1099-1492(1998110)11:7<341::aid-nbm5
更新日期:1998-11-01 00:00:00
abstract::31P Nuclear magnetic resonance spectroscopy has been used to investigate the combined effect of 70 mM ethanol administration and hypoxia on the energetic metabolism and cytosolic pH of the perfused liver isolated from naive, fasted or chronically ethanol-treated rats. Ethanol-treatment or fasting induce a similar 25% ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940030306
更新日期:1990-06-01 00:00:00
abstract::Recent work has shown that solid-state (1) H and (31) P MRI can provide detailed insight into bone matrix and mineral properties, thereby potentially enabling differentiation of osteoporosis from osteomalacia. However, (31) P MRI of bone mineral is hampered by unfavorable relaxation properties. Hence, accurate knowled...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2930
更新日期:2013-09-01 00:00:00
abstract::A special characteristic of the brain is the usage of lactate as alternative fuel instead of glucose to preserve its energy homeostasis. This physiological function is valid for sufficient cerebral glucose supply, as well as presumably during hypoglycemia, given that exogenous lactate infusion suppresses hormonal coun...
journal_title:NMR in biomedicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/nbm.4167
更新日期:2019-11-01 00:00:00
abstract::The leakage of metabolites from frozen and thawed tissue biopsies was measured semi-quantitatively by high-field (8.5 T) proton MRS. Human prostate and rat brain tissue specimens, frozen within 1 min of collection, lost significant and variable amounts of diagnostic metabolites immediately upon thawing. For prostate t...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.815
更新日期:2003-04-01 00:00:00
abstract::Proton magnetic resonance spectroscopy at 1.9 T was used to quantify dolichols, cholesterols, choline-containing phospholipids and double bonds in unsaturated acyl chains in lipid extracts of four types of thyroid tissue [normal (n = 27), papillary cancer (n = 15), adenoma (n = 13) and Basedow disease (n = 6)]. In nor...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/1099-1492(200011)13:7<377::aid-nbm658>3.0.
更新日期:2000-11-01 00:00:00
abstract::T1ρ relaxation imaging is a quantitative imaging technique that has been used to assess cartilage integrity, liver fibrosis, tumors, cardiac infarction, and Alzheimer's disease. T1 , T2 , and T1ρ relaxation time constants have each demonstrated different degrees of sensitivity to several markers of fibrosis and inflam...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4284
更新日期:2020-05-01 00:00:00