Abstract:
:The applications of in vivo electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy have been impressive over a relatively short period despite the many obstacles which had to be overcome, such as dielectric absorption and biodestruction. The loop-gap resonators and modified loop coil systems have emerged as the most suitable resonators for in vivo EPR experiments. This paper briefly discusses instrumental aspects as a prelude to several examples related to the in vivo monitoring and detection of bioradicals. Recent progress in detection of bioradicals induced by drugs or chemicals is discussed with regard to nitrosocompounds, nitric oxide and metals in vivo. A clinical EPR application is also discussed.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Fujii H,Berliner LJdoi
10.1002/nbm.898subject
Has Abstractpub_date
2004-08-01 00:00:00pages
311-8issue
5eissn
0952-3480issn
1099-1492journal_volume
17pub_type
杂志文章,评审abstract::The purpose of this study was to investigate the reliability of 3D isotropic MRI and quantitative multi-parametric MRI characterization on an orthotopic pancreatic head tumor model in rats. 3D isotropic T2 -weighted MRI was performed as a routine for tumor longitudinal follow-up and volume estimation. Common bile duct...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3676
更新日期:2017-02-01 00:00:00
abstract::In vivo metabolic imaging using hyperpolarized [1-(13)C]pyruvate provides localized biochemical information and is particularly useful in detecting early disease changes, as well as monitoring disease progression and treatment response. However, a major limitation of hyperpolarized magnetization is its unrecoverable d...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3346
更新日期:2015-08-01 00:00:00
abstract:PURPOSE:To develop a novel respiratory motion compensated three-dimensional (3D) cardiac magnetic resonance fingerprinting (cMRF) approach for whole-heart myocardial T1 and T2 mapping from a free-breathing scan. METHODS:Two-dimensional (2D) cMRF has been recently proposed for simultaneous, co-registered T1 and T2 mapp...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4370
更新日期:2020-10-01 00:00:00
abstract::MR Z-spectroscopy allows enhanced imaging contrast on the basis of saturation transfer between the proton pools of cellular compounds and water, occurring via chemical exchange (chemical exchange saturation transfer, CEST) or dipole-dipole coupling (nuclear Overhauser effect, NOE). In previous studies, signals observe...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3021
更新日期:2013-12-01 00:00:00
abstract::One of the most commonly used methods for in vivo MRS detection of γ-aminobutyric acid (GABA) is the MEGA-point-resolved spectroscopy (MEGA-PRESS) technique. However, accurate quantification of GABA using MEGA-PRESS is complicated by spectral co-editing of macromolecular resonances. In this article, a new pulse sequen...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1688
更新日期:2011-12-01 00:00:00
abstract::Although diffusion MRI has shown promise for the characterization of breast cancer, it has low specificity to malignant subtypes. Higher specificity might be achieved if the effects of cell morphology and molecular exchange across cell membranes could be disentangled. The quantification of exchange might thus allow th...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3504
更新日期:2016-05-01 00:00:00
abstract::Free cytosolic [Mg2+] can be assessed in vivo by 31P MRS from the chemical shift of beta-ATP which in turn depends on the fraction of total ATP complexed to Mg2+ ions. The reliability of these in vivo measurements depends on the availability of an appropriate in vitro calibration to determine the limits of chemical sh...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199602)9:1<24::AID-NBM392>
更新日期:1996-02-01 00:00:00
abstract::Nuclear Overhauser enhancement (NOE) is a type of magnetization transfer using cross-relaxation. It originates from mobile macromolecules, which may have relevance to the evaluation of tumor features. We studied the value of NOE imaging at 7 and 3 T and suggest a utility for diagnosing human brain tumors. Two types of...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3735
更新日期:2017-09-01 00:00:00
abstract::In this study, we have performed simulations to address the effects of diffusion encoding parameters, signal-to-noise ratio (SNR) and T2 on skeletal muscle diffusion tensor indices and fiber tracts. Where appropriate, simulations were corroborated and validated by in vivo diffusion tensor imaging (DTI) of human skelet...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2959
更新日期:2013-11-01 00:00:00
abstract::The processes of tumor invasion and metastasis have been well characterized at the molecular level, and numerous biomarkers of tumor aggressiveness have been discovered. Molecular imaging offers the opportunity to depict specific cell markers relevant to tumor aggressiveness. Here, we describe the role of MRI in ident...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1590
更新日期:2011-07-01 00:00:00
abstract::In this study the conjugated macromolecular ligand, diethylenetriaminepentaacetic acid (DTPA)-amide-dextran, was synthesized by attaching DTPA to the dextran macromolecule (M(r) approximately 6000) by a covalent amide bond. Subsequently, DTPA-amide-dextran was complexed with either of the two lanthanide metal ions dys...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940080404
更新日期:1995-06-01 00:00:00
abstract::Citicoline supplementation has been used to ameliorate memory disturbances in older people and those with Alzheimer's disease. This study used MRS to characterize the effects of citicoline on high-energy phosphate metabolites and constituents of membrane synthesis in the frontal lobe. Phosphorus ((31)P) metabolite dat...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1281
更新日期:2008-11-01 00:00:00
abstract::Spatiotemporally structured noise, such as physiological noise, is a potential source of artifacts in functional magnetic resonance imaging (fMRI) and is the main limiting factor for the detection of small blood oxygen level-dependent (BOLD) signal variations. fMRI was employed to detect low-frequency BOLD signal fluc...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.987
更新日期:2005-12-01 00:00:00
abstract::The goals of this study were to develop an acquisition protocol and the analysis tools for Meshcher-Garwood point-resolved spectroscopy (MEGA-PRESS) in mouse brain at 9.4 T, to allow the in vivo detection of γ-aminobutyric acid (GABA) and to examine whether isoflurane alters GABA levels in the thalamus during anesthes...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3837
更新日期:2018-01-01 00:00:00
abstract::Detailed (1)H and (13)C NMR spectroscopy of lipid extracts from 12 human intracranial tuberculomas and two control brain tissue samples was performed to assess the role of lipids in the disease process. One-dimensional and two-dimensional NMR techniques were used to resolve the mixture of lipid components and make res...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1191
更新日期:2008-03-01 00:00:00
abstract::Given the importance of genetically modified mice in studies of mammalian brain development and human congenital brain diseases, MRI has the potential to provide an efficient in vivo approach for analyzing mutant phenotypes in the early postnatal mouse brain. The combination of reduced tissue contrast at the high magn...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.932
更新日期:2004-12-01 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is characterized at histology by steatosis, hepatocyte ballooning and inflammatory infiltrates, with or without fibrosis. Although diamagnetic material in fibrosis and inflammation can be detected with quantitative susceptibility imaging, fatty acid composition changes in NASH rela...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3766
更新日期:2017-10-01 00:00:00
abstract::Hyperpolarised (HP) (13)C NMR allows enzymatic activity to be probed in real time in live biological systems. The use of in vitro models gives excellent control of the cellular environment, crucial in the understanding of enzyme kinetics. The increased conversion of pyruvate to lactate in cancer cells has been well st...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3341
更新日期:2015-08-01 00:00:00
abstract::Bone contains a significant fraction of water that is not detectable with ordinary Cartesian imaging sequences. The advent of ultra-short echo-time (UTE) methods has allowed the recovery of this submillisecond T(2)* water. In this work, we have developed a new three-dimensional hybrid-radial ultra-short echo-time (3D ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1631
更新日期:2011-08-01 00:00:00
abstract::Glycine (Gly) has been implicated in several neurological disorders, including malignant brain tumors. The precise measurement of Gly is challenging largely as a result of the spectral overlap with myo-inositol (mI). We report a new triple-refocusing sequence for the reliable co-detection of Gly and mI at 3 T and for ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3747
更新日期:2017-09-01 00:00:00
abstract::The accurate diagnosis of glioma subtypes is critical for appropriate treatment, but conventional histopathologic diagnosis often exhibits significant intra-observer variability and sampling error. The aim of this study was to investigate whether histogram analysis using an automatically segmented region of interest (...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3153
更新日期:2014-09-01 00:00:00
abstract::Here, we show that the sensitivity of (31)P MRSI of (31)P spins J-coupled to protons can be increased by almost a factor of three when compared with an optimal direct detection free induction decay. By direct detection integrated with multi-echo polarization transfer (DIMEPT), multiple signals from polarization transf...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3183
更新日期:2014-10-01 00:00:00
abstract::Signal of lung parenchymal tissue from the living rat and mouse lung was detected at 4.7 T with a good signal-to-noise ratio and motion-suppressed artifacts using a short TE gradient-echo sequence. Neither cardiac nor respiratory gating were applied, and animals respired freely during data collection. Mean T(2)* relax...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.706
更新日期:2001-08-01 00:00:00
abstract::A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940050605
更新日期:1992-11-01 00:00:00
abstract::Magnetic resonance imaging (MRI) in pregnancy is commonly undertaken in the left lateral tilt (LLT) position to prevent inferior vena cava (IVC) compression and supine hypotensive events, although this may be suboptimal for image quality. The supine position may also have an adverse effect on fetal well-being. The spi...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4475
更新日期:2021-01-21 00:00:00
abstract::Owing to the small quantity of tissue available in human biopsy specimens, aqueous and lipid components often have to be determined in the same tissue sample. Perchloric acid (PCA) used for the extraction of aqueous metabolites has a deleterious effect on lipid components; the severity of the damage is not known. In t...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1159
更新日期:2008-02-01 00:00:00
abstract::The management and treatment of high-grade glioblastoma multiforme (GBM) and solitary metastasis (MET) are very different and influence the prognosis and subsequent clinical outcomes. In the case of a solitary MET, diagnosis using conventional radiology can be equivocal. Currently, a definitive diagnosis is based on h...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3163
更新日期:2014-09-01 00:00:00
abstract::Encoding of spatial information in magnetic resonance imaging is conventionally accomplished by using magnetic field gradients. During gradient encoding, the position in k-space is determined by a time-integral of the gradient field, resulting in a limitation in imaging speed due to either gradient power or secondary ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1399
更新日期:2009-11-01 00:00:00
abstract::In this work, we report a case study of a stroke model in animals using two methods of quantification of the deviations from Gaussian behaviour: diffusion kurtosis imaging (DKI) and log-normal distribution function imaging (LNDFI). The affected regions were predominantly in grey rather than in white matter. The parame...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2802
更新日期:2012-11-01 00:00:00
abstract::Progress in the development of non-proton NMR applications in medical research has been hampered by the generally low sensitivity of these nuclides as well as the lack of instrumentation readily available for studying these spins. For example, custom head coils suitable for sodium imaging of the human head are either ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940020308
更新日期:1989-09-01 00:00:00