Abstract:
:Given the importance of genetically modified mice in studies of mammalian brain development and human congenital brain diseases, MRI has the potential to provide an efficient in vivo approach for analyzing mutant phenotypes in the early postnatal mouse brain. The combination of reduced tissue contrast at the high magnetic fields required for mice, and the changing cellular composition of the developing mouse brain make it difficult to optimize MRI contrast in neonatal mouse imaging. We have explored an easily implemented approach for contrast-enhanced imaging, using systemically administered manganese (Mn) to reveal fine anatomical detail in T1-weighted MR images of neonatal mouse brains. In particular, we demonstrate the utility of this Mn-enhanced MRI (MEMRI) method for analyzing early postnatal patterning of the mouse cerebellum. Through comparisons with matched histological sections, we further show that MEMRI enhancement correlates qualitatively with granule cell density in the developing cerebellum, suggesting that the cerebellar enhancement is due to uptake of Mn in the granule neurons. Finally, variable cerebellar defects in mice with a conditional mutation in the Gbx2 gene were analyzed with MEMRI to demonstrate the utility of this method for mutant mouse phenotyping. Taken together, our results indicate that MEMRI provides an efficient and powerful in vivo method for analyzing neonatal brain development in normal and genetically engineered mice.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Wadghiri YZ,Blind JA,Duan X,Moreno C,Yu X,Joyner AL,Turnbull DHdoi
10.1002/nbm.932subject
Has Abstractpub_date
2004-12-01 00:00:00pages
613-9issue
8eissn
0952-3480issn
1099-1492journal_volume
17pub_type
杂志文章abstract::Elevated brain myo-inositol (m-Ins) concentration (a putative marker of neuroinflammation) has been reported in patients suffering from type 2 diabetes mellitus (T2DM). Obesity alone and T2DM have been found to be associated with a lower concentration of N-acetyloaspartate and N-acetylaspartylglutamate (tNAA, a marker...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3957
更新日期:2018-09-01 00:00:00
abstract::Magnetic resonance imaging (MRI) in pregnancy is commonly undertaken in the left lateral tilt (LLT) position to prevent inferior vena cava (IVC) compression and supine hypotensive events, although this may be suboptimal for image quality. The supine position may also have an adverse effect on fetal well-being. The spi...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4475
更新日期:2021-01-21 00:00:00
abstract::The purpose of this work was to assess the reproducibility of percentage of ventilated lung volume (PV) measured from hyperpolarized (HP) (3)He and (1)H anatomical images acquired in the same breath-hold when compared with PV measured from (3)He and (1)H images from separate breath-holds. Volumetric (3)He ventilation ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3187
更新日期:2014-12-01 00:00:00
abstract::In many studies, it has been demonstrated that (1)H MRSI of the human prostate has great potential to aid prostate cancer management, e.g. in the detection and localisation of cancer foci in the prostate or in the assessment of its aggressiveness. It is particularly powerful in combination with T2 -weighted MRI. Never...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.2973
更新日期:2014-01-01 00:00:00
abstract::Prader-Willi syndrome (PWS) is a genetic imprinting disorder characterized mainly by hyperphagia and early childhood obesity. Previous functional neuroimaging studies used visual stimuli to examine abnormal activities in the eating-related neural circuitry of patients with PWS. It was found that patients with PWS exhi...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2900
更新日期:2013-06-01 00:00:00
abstract::Patients with different types of mucopolysaccharidoses (MPS) lack specific lysosomal enzymes, which leads to tissue accumulation and urinary excretion of glycosaminoglycans (GAGs). Since little is known about the molecular composition of the excreted GAG fragments, we used two-dimensional [1H,13C]-correlation nuclear ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.833
更新日期:2003-06-01 00:00:00
abstract::MRI and MRS in small rodents demand very high sensitivity. Cryogenic transmit/receive radiofrequency probes (CryoProbes) designed for (1) H MRI of mouse brain provide an attractive option for increasing the performance of small-animal MR systems. As the Larmor frequency of (13) C nuclei is four times lower than that f...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3110
更新日期:2014-06-01 00:00:00
abstract::Chemical exchange saturation transfer (CEST) can offer information about protons associated with mobile proteins through the amide proton transfer (APT) effect, which has been shown to discriminate tumor from healthy tissue and, more recently, has been suggested as a prognosticator of response to therapy. Despite this...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2947
更新日期:2013-10-01 00:00:00
abstract::The combination of hyperpolarized Xe with chemical exchange saturation transfer (Hyper-CEST) is a powerful NMR technique to detect highly dilute concentrations of Xe binding sites using RF saturation pulses. Crucially, that combination of saturation pulse strength and duration that generates the maximal Hyper-CEST eff...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3307
更新日期:2015-06-01 00:00:00
abstract::This paper describes the use of off-resonance saturation to further manipulate the blood oxygenation level dependent (BOLD) contrast of fMRI. A customized narrow bandwidth radiofrequency pulse, applied with a range of frequency offsets prior to selection of each slice, was designed and incorporated into a gradient ech...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199706/08)10:4/5<208::aid-
更新日期:1997-06-01 00:00:00
abstract::The aim of the study was to establish the normal range and to evaluate the reproducibility of dynamic contrast enhanced MRI (DCE-MRI) parameter estimates in normal human pelvic tissues. Nineteen patients with prostate cancer, undergoing androgen deprivation treatment, had paired DCE-MRI examinations of the pelvis usin...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.732
更新日期:2002-04-01 00:00:00
abstract::Brain myelin and iron content are important parameters in neurodegenerative diseases such as multiple sclerosis (MS). Both myelin and iron content influence the brain's R2* relaxation rate. However, their quantification based on R2* maps requires a realistic tissue model that can be fitted to the measured data. In str...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4092
更新日期:2019-07-01 00:00:00
abstract::(1) H-MRS is regularly applied to determine lipid content in ectopic tissue - mostly skeletal muscle and liver - to investigate physiological and/or pathologic conditions, e.g. insulin resistance. Technical developments also allow non-invasive in vivo assessment of cardiac lipids; however, basic data about methodologi...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3186
更新日期:2014-11-01 00:00:00
abstract::Chemical exchange saturation transfer (CEST) potentially provides the ability to detect small solute pools through indirect measurements of attenuated water signals. However, CEST effects may be diluted by various competing effects, such as non-specific magnetization transfer (MT) and asymmetric MT effects, water long...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3243
更新日期:2015-02-01 00:00:00
abstract::Progress in the development of non-proton NMR applications in medical research has been hampered by the generally low sensitivity of these nuclides as well as the lack of instrumentation readily available for studying these spins. For example, custom head coils suitable for sodium imaging of the human head are either ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940020308
更新日期:1989-09-01 00:00:00
abstract::Balanced steady-state free precession imaging has recently been suggested for chemical exchange detection (bSSFPX). The objective of this work is to investigate the contributions of microstructural, chemical shift and chemical exchange effects to the asymmetry of the bSSFP profile at field strengths of 3 T and 9.4 T. ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4200
更新日期:2020-03-01 00:00:00
abstract::To investigate the GABA+ modeling accuracy of MEGA-PRESS GABA+-edited MRS data with various spectral quality scenarios, the influence of varying signal-to-noise ratio (SNR) and linewidth on the model estimates was quantified. MEGA-PRESS data from 46 volunteers were averaged to generate a template MEGA-PRESS spectrum, ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4410
更新日期:2021-01-01 00:00:00
abstract::13C MRS was installed on a clinical scanner at 1.5 T in order to facilitate integrated MR examinations of human brain disorders. Using a simplified protocol, (1-(13)C) glucose and/or (1-(13)C) acetate were administered orally or by intravenous infusion. (13)C spectra of diagnostic quality were acquired in more than 10...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.852
更新日期:2003-10-01 00:00:00
abstract::Hyperpolarised (HP) (13)C NMR allows enzymatic activity to be probed in real time in live biological systems. The use of in vitro models gives excellent control of the cellular environment, crucial in the understanding of enzyme kinetics. The increased conversion of pyruvate to lactate in cancer cells has been well st...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3341
更新日期:2015-08-01 00:00:00
abstract::Diffusion measurements were performed on the N-acetyl aspartate (NAA) signal in in situ brains (in vivo and post-mortem) and on in vitro brain tissue at 37 degrees C using wide ranges of b-values (from 0 up to 4.5 x 10(6) s/cm2 and 35.8 x 10(6) s/cm2 for the in vivo and the in vitro cases, respectively). In vivo and i...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199804)11:2<67::aid-nbm503
更新日期:1998-04-01 00:00:00
abstract::The possibility of quantifying the superimposed signal of glutamate and glutamine (Glx) and its components by ¹H magnetic resonance spectroscopy (MRS) in the spinal cord is an exciting challenge with important clinical applications in neurological conditions. The spinal cord is a particularly difficult region of inter...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2867
更新日期:2013-03-01 00:00:00
abstract::Blood flow and phosphorus metabolites have been measured simultaneously in the murine RIF-1 tumour in vivo. Blood flow was measured using the H2 washout technique and 31P NMR spectroscopy was used to measure high energy phosphates, inorganic phosphate and intracellular pH within the tumour. Following NMR and flow meas...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940030406
更新日期:1990-08-01 00:00:00
abstract::Here, we show that the sensitivity of (31)P MRSI of (31)P spins J-coupled to protons can be increased by almost a factor of three when compared with an optimal direct detection free induction decay. By direct detection integrated with multi-echo polarization transfer (DIMEPT), multiple signals from polarization transf...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3183
更新日期:2014-10-01 00:00:00
abstract::A study was undertaken to determine if the vascular characteristics measured by dynamic contrast-enhanced magnetic resonance imaging (primarily permeability surface area product and extracellular-extravascular tissue volume fraction) would be beneficial in explaining the inter-lesion metabolic heterogeneity displayed ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.675
更新日期:2000-12-01 00:00:00
abstract::The signal decay with increasing b-factor at fixed echo time from brain tissue in vivo has been measured using a line scan Stejskal-Tanner spin echo diffusion approach in eight healthy adult volunteers. The use of a 175 ms echo time and maximum gradient strengths of 10 mT/m allowed 64 b-factors to be sampled, ranging ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199902)12:1<51::aid-nbm546
更新日期:1999-02-01 00:00:00
abstract::One of the most commonly used methods for in vivo MRS detection of γ-aminobutyric acid (GABA) is the MEGA-point-resolved spectroscopy (MEGA-PRESS) technique. However, accurate quantification of GABA using MEGA-PRESS is complicated by spectral co-editing of macromolecular resonances. In this article, a new pulse sequen...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1688
更新日期:2011-12-01 00:00:00
abstract::Metabolic activity in the lung is known to change in response to external insults, inflammation, and cancer. We report measurements of metabolism in the isolated, perfused rat lung of healthy controls and in diseased lungs undergoing acute inflammation using hyperpolarized 1-(13) C-labeled pyruvate. The overall appare...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3139
更新日期:2014-08-01 00:00:00
abstract::Results are presented from an in vivo study of human skin in which a zig-zag surface coil together with a three-pulse Fourier Series Window (FSW) protocol have been used to obtain minimally contaminated 31P skin spectra of the posterior calf. Phantom experiments indicate that while the fall-off in the B1 field profile...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199608)9:5<195::AID-NBM416
更新日期:1996-08-01 00:00:00
abstract::Bone contains a significant fraction of water that is not detectable with ordinary Cartesian imaging sequences. The advent of ultra-short echo-time (UTE) methods has allowed the recovery of this submillisecond T(2)* water. In this work, we have developed a new three-dimensional hybrid-radial ultra-short echo-time (3D ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1631
更新日期:2011-08-01 00:00:00
abstract::Increased pulmonary lactate production is correlated with severity of lung injury and outcome in acute respiratory distress syndrome (ARDS) patients. This study was conducted to investigate the relative contributions of inflammation and hypoxia to the lung's metabolic shift to glycolysis in an experimental animal mode...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4380
更新日期:2020-11-01 00:00:00