Abstract:
:Proton magnetic resonance spectroscopy at 1.9 T was used to quantify dolichols, cholesterols, choline-containing phospholipids and double bonds in unsaturated acyl chains in lipid extracts of four types of thyroid tissue [normal (n = 27), papillary cancer (n = 15), adenoma (n = 13) and Basedow disease (n = 6)]. In normal thyroid the mean concentrations of dolichol, cholesterol and phospholipids were 1.2, 3.6 and 2.1 micromol/g wet weight, respectively. The concentrations of these lipids exhibited positive mutual correlations and positive correlations with patient age. The increase in dolichol in elderly human thyroid may be due to the accumulation of lysosomes and may help to compensate for the decrease in the activity of lysosomal enzymes and in thyroid hormone production and release. Dolichol concentrations were significantly lower in papillary cancer (0.4 micromol/g) and Basedow disease (0.3 micromol/g) compared to normal thyroid (p < 0.01 and p < 0.05, respectively), while cholesterol was enhanced only in cancer tissue (10.7 micromol/g). Benign adenoma exhibited normal levels of both dolichol and cholesterol. These results suggest that the synthesis and accumulation of isoprenoids are normal in adenoma but not in cancer.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Yoshioka Y,Sasaki J,Yamamoto M,Saitoh K,Nakaya S,Kubokawa Mdoi
10.1002/1099-1492(200011)13:7<377::aid-nbm658>3.0.subject
Has Abstractpub_date
2000-11-01 00:00:00pages
377-83issue
7eissn
0952-3480issn
1099-1492pii
10.1002/1099-1492(200011)13:7<377::AID-NBM658>3.0.journal_volume
13pub_type
杂志文章abstract::Net ATP production rates in exercising human muscle were calculated from 31P MRS kinetic data collected with 1-8 s time resolution. During the exercise, the muscle output was measured using a force transducer. Muscle output (force/unit volume) is then divided by the calculated net ATP used to estimate the ATP cost of ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940040404
更新日期:1991-08-01 00:00:00
abstract::By combining intravoxel incoherent motion (IVIM) and diffusion tensor imaging (DTI) we introduce a new diffusion model called intravoxel oriented flow (IVOF) that accounts for anisotropy of diffusion and the flow-related signal. An IVOF model using a simplified apparent flow fraction tensor (IVOFf ) is applied to diff...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3584
更新日期:2016-10-01 00:00:00
abstract::This paper describes the development of a novel technique for functional MRI of the human brain at 0.135 microL resolution for a whole brain section. In comparison with conventional studies at 3 mm isotropic resolution or 27 microL voxel size, the method yields an improvement by a factor of 200. To achieve optimum ima...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.998
更新日期:2005-12-01 00:00:00
abstract::While phase imaging with a gradient echo (GRE) sequence is popular, phase imaging with balanced steady-state free precession (bSSFP) has been underexplored. The purpose of this study was to investigate anatomical and functional phase imaging with multiple phase-cycled bSSFP, in expectation of increasing spatial covera...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3699
更新日期:2017-06-01 00:00:00
abstract::The physiological status of anesthetized rats greatly influences blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI). Monitoring of physiological parameters, particularly partial pressure of carbon dioxide (pCO(2)) levels in the blood, is therefore an important part in the design and ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.978
更新日期:2005-11-01 00:00:00
abstract::MRI detects changes in blood-oxygenation-level dependent (BOLD) contrast in tumors caused by tumor oxygenating agents. These changes can be used to guide the design of improved tumor oxygenating treatments (TOXs). The conventional approach to detection of BOLD effects assumes that the water resonance is a single, homo...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.728
更新日期:2002-02-01 00:00:00
abstract::High-resolution MR imaging (312 microns in plane resolution) and MR spectroscopy (0.36 cm3 nominal voxel) have been performed on human frontal cortex using a 3 in surface coil. Localized proton spectra have been obtained from contiguous 6 x 6 x 10 mm voxels using one-dimensional phase encoding, TR 2000 ms and TE 31 ms...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(SICI)1099-1492(199610)9:7<297::AID-NBM433
更新日期:1996-10-01 00:00:00
abstract::Evaluation of high intensity focused ultrasound (HIFU) treatment with MRI is generally based on assessment of the non-perfused volume from contrast-enhanced T1-weighted images. However, the vascular status of tissue surrounding the non-perfused volume has not been extensively investigated with MRI. In this study, clus...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3406
更新日期:2015-11-01 00:00:00
abstract::Increased sensitivity and chemical shift dispersion at ultra-high magnetic fields enable the precise quantification of an extended range of brain metabolites from (1)H MRS. However, all previous neurochemical profiling studies using single-voxel MRS at 7 T have been limited to data acquired from the occipital lobe wit...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1727
更新日期:2012-01-01 00:00:00
abstract::The reliability, relation to whole-body maximal oxygen uptake (VO(2max)), and force-level sensitivity of (31)P MRS markers of mitochondrial function were studied in 39 normal-weight women. Following 90 s isometric plantar-flexion exercises at 45, 70 and 100% of maximum voluntary contraction, skeletal muscle mitochondr...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(200002)13:1<14::aid-nbm605
更新日期:2000-01-01 00:00:00
abstract::The relaxation properties of water and metabolites were measured in rat brain following the occlusion of the middle cerebral artery (MCA) with localized 1H MRS. The PRESS sequence was employed to select volumes of 39 microL in the ischemic and the contralateral hemisphere. T1 and T2 relaxation times and peak intensiti...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940080604
更新日期:1995-09-01 00:00:00
abstract::Resolution enhancement for glutamate (Glu), glutamine (Gln) and glutathione (GSH) in the human brain by TE-optimized point-resolved spectroscopy (PRESS) at 7 T is reported. Sub-TE dependences of the multiplets of Glu, Gln, GSH, γ-aminobutyric acid (GABA) and N-acetylaspartate (NAA) at 2.2-2.6 ppm were investigated wit...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1529
更新日期:2010-11-01 00:00:00
abstract::Studies of cholesteryl ester transfer protein (CETP) function in lipoprotein mixtures pose many difficulties by conventional biochemical methods. For instance, studies on the effects of CETP on the composition of apolipoprotein B containing lipoproteins (very low and low density lipoproteins) in lipoprotein mixtures a...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199710)10:7<303::aid-nbm48
更新日期:1997-10-01 00:00:00
abstract::Early imaging or blood biomarkers of tumor response is needed to customize anti-tumor therapy on an individual basis. This study evaluates the sensitivity and relevance of five potential MRI biomarkers. Sixty nude rats were implanted with human glioma cells (U-87 MG) and randomized into three groups: one group receive...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1611
更新日期:2011-06-01 00:00:00
abstract::The combination of hyperpolarized Xe with chemical exchange saturation transfer (Hyper-CEST) is a powerful NMR technique to detect highly dilute concentrations of Xe binding sites using RF saturation pulses. Crucially, that combination of saturation pulse strength and duration that generates the maximal Hyper-CEST eff...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3307
更新日期:2015-06-01 00:00:00
abstract::The accurate diagnosis of glioma subtypes is critical for appropriate treatment, but conventional histopathologic diagnosis often exhibits significant intra-observer variability and sampling error. The aim of this study was to investigate whether histogram analysis using an automatically segmented region of interest (...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3153
更新日期:2014-09-01 00:00:00
abstract::In vivo 1H MRS is rapidly developing as a clinical tool for diagnosing and characterizing breast cancers. Many in vivo and in vitro experiments have demonstrated that alterations in concentrations of choline-containing metabolites are associated with malignant transformation. In recent years, considerable efforts have...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1217
更新日期:2009-01-01 00:00:00
abstract::A novel and fast time-domain quantitation algorithm--quantitation based on semi-parametric quantum estimation (QUEST)--invoking optimal prior knowledge is proposed and tested. This nonlinear least-squares algorithm fits a time-domain model function, made up from a basis set of quantum-mechanically simulated whole-meta...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.895
更新日期:2005-02-01 00:00:00
abstract::This study investigated the association between the total choline (tCho) concentration and the clinical characteristics and biomarker status of breast cancer. Sixty-two patients with breast cancer, 1.5 cm or larger in size on MR images, were studied. The tCho concentration was correlated with the MRI features, contra...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1595
更新日期:2011-04-01 00:00:00
abstract::Non-alcoholic steatohepatitis (NASH) is characterized at histology by steatosis, hepatocyte ballooning and inflammatory infiltrates, with or without fibrosis. Although diamagnetic material in fibrosis and inflammation can be detected with quantitative susceptibility imaging, fatty acid composition changes in NASH rela...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3766
更新日期:2017-10-01 00:00:00
abstract::The application of 31P MR spectroscopy in the characterization and treatment of malignant human extremity tumors is reviewed and placed in the perspective of results obtained in murine sarcomas. Despite the now widespread acquisition of gradient localized spectral maps, the low spatial resolution that can be achieved ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/(sici)1099-1492(1998110)11:7<341::aid-nbm5
更新日期:1998-11-01 00:00:00
abstract::Hippocampal dysfunction is known to be associated with several neurological and neuropsychiatric disorders such as Alzheimer's disease, epilepsy, schizophrenia and depression; therefore, there has been significant clinical interest in studying hippocampal neurochemistry. However, the hippocampus is a challenging regio...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3309
更新日期:2015-06-01 00:00:00
abstract::In recent years considerable effort has been devoted to applying pattern recognition techniques to the complex task of data analysis in magnetic resonance spectroscopy. It may be argued that such techniques will facilitate putting MRS technology to practical clinical use. This paper reviews approaches of pattern recog...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/(sici)1099-1492(199705)10:3<99::aid-nbm461
更新日期:1997-05-01 00:00:00
abstract::One of the most commonly used methods for in vivo MRS detection of γ-aminobutyric acid (GABA) is the MEGA-point-resolved spectroscopy (MEGA-PRESS) technique. However, accurate quantification of GABA using MEGA-PRESS is complicated by spectral co-editing of macromolecular resonances. In this article, a new pulse sequen...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1688
更新日期:2011-12-01 00:00:00
abstract::(31)P MRS provides a unique non-invasive window into myocardial energy homeostasis. Mouse models of cardiac disease are widely used in preclinical studies, but the application of (31)P MRS in the in vivo mouse heart has been limited. The small-sized, fast-beating mouse heart imposes challenges regarding localized sign...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3371
更新日期:2015-10-01 00:00:00
abstract::The availability of high-field-strength magnetic resonance imaging (MRI) systems has brought about the development of techniques that aim to map myelination via the exploitation of various contrast mechanisms. Myelin mapping techniques have the potential to provide tools for the diagnosis and treatment of diseases, su...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3877
更新日期:2018-03-01 00:00:00
abstract::A short-echo spectroscopic imaging sequence extended with a frequency-selective multiple-quantum- coherence technique (Sel-MQC) is presented. The method enables acquisition of a complete water-suppressed proton spectrum with a short echo time and filtering of the J-coupling metabolite, lactate, from co-resonant lipids...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1284
更新日期:2008-11-01 00:00:00
abstract::An indirect method for in vivo T2 mapping of 13 C-labeled metabolites using T2 and T2 * information of water protons obtained a priori is proposed. The T2 values of 13 C metabolites are inferred using the relationship to T2 ' of coexisting 1 H and the T2 * of 13 C metabolites, which is measured using routine hyperpola...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3690
更新日期:2017-05-01 00:00:00
abstract::Modified gradient-echo MR techniques were applied to study the effects of inflow on functional brain imaging studies using visual and motor cortex stimulation. The results demonstrate that the large signal changes, seen in previously reported gradient-echo studies at 1.5-2.0 T, are dominated by direct inflow effects, ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940070113
更新日期:1994-03-01 00:00:00
abstract::The aim of this study was to acquire high-quality in vivo (1) H spectra concurrently from two voxels at ultra-high field (7 T) without specialized hardware. To this end, an acquisition scheme was developed in which first-order shims and flip angles are dynamically updated to acquire spectra from both of the brain's mo...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3328
更新日期:2015-07-01 00:00:00