Abstract:
:Studies of cholesteryl ester transfer protein (CETP) function in lipoprotein mixtures pose many difficulties by conventional biochemical methods. For instance, studies on the effects of CETP on the composition of apolipoprotein B containing lipoproteins (very low and low density lipoproteins) in lipoprotein mixtures are tedious due to repeated ultracentrifugational isolations and have thus rarely been performed. Here we present a new 1H NMR spectroscopy technique to assess the CETP function in lipoprotein mixtures. This technique does not require repeated physical isolations of the lipoprotein particles but uses mathematical separation of the fractions on the basis of biochemical prior knowledge based lineshape fitting analysis of specific lipid resonances in the 1H NMR spectra. The lipoproteins are separated according to their size related chemical shift which allows for distinct quantification between very low and low density lipoproteins, the two major apolipoprotein B containing fractions. The methodological basis of the technique is discussed here together with a demonstration that this kind of approach allows dynamic follow up of the lipid transfer reactions in complex lipoprotein and CETP mixtures. The results revealed a consistent behaviour which corroborated the recent findings suggesting that the neutral lipid mass transfer among lipoproteins is not an equimolar heteroexchange.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
Korhonen A,Ala-Korpela M,Liinamaa MJ,Jokisaari J,Kesäniemi YA,Savolainen MJdoi
10.1002/(sici)1099-1492(199710)10:7<303::aid-nbm48subject
Has Abstractpub_date
1997-10-01 00:00:00pages
303-8issue
7eissn
0952-3480issn
1099-1492pii
10.1002/(SICI)1099-1492(199710)10:7<303::AID-NBM48journal_volume
10pub_type
杂志文章abstract::In this study, Blood Oxygenation Level Dependent (BOLD) contrast in the detection of human brain activation was compared between spin-echo and gradient-echo echo-planar sequences at 1.5 T. Time course series of spin-echo and gradient-echo images containing the primary motor cortex were collected during rest (no finger...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940070104
更新日期:1994-03-01 00:00:00
abstract::Traditional methods of measuring magnetization in magnetic fluid samples, such as vibrating sample magnetometry (VSM), are typically limited to maximum field strengths of about 1 T. This work demonstrates the ability of MRI to measure the magnetization associated with two commercial MRI contrast agents at 3 T by compa...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1412
更新日期:2009-10-01 00:00:00
abstract::This paper compares two spectral processing methods for obtaining quantitative measures from in vivo prostate spectra, evaluates their effectiveness, and discusses the necessary modifications for accurate results. A frequency domain analysis (FDA) method based on peak integration was compared with a time domain fittin...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1008
更新日期:2006-04-01 00:00:00
abstract::This study tests the hypothesis that brief periods of ischemia result in an increase in myocardial lipids during early reperfusion. We conducted 1H NMR spectroscopy to serially measure myocardial lipids before and after regional ischemia. Localized 1H NMR spectra (spatial resolution of 1.25 mm) were obtained using a o...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940080206
更新日期:1995-04-01 00:00:00
abstract::We propose a very high-resolution, three-dimensional (3D) gradient-echo technique with a twofold parallel imaging acceleration using a specialized occipital receiver coil at 3 T to perform functional MRI (fMRI) of the visual cortex. This configuration makes it possible to acquire 3D fMRI data within a timescale compat...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1158
更新日期:2007-08-01 00:00:00
abstract::Acute kidney injury (AKI) in mice caused by sustained ischemia followed by reperfusion is associated with acute tubular necrosis and renal dysfunctional blood flow. Although the principal role of the kidney is the maintenance of acid-base balance, current imaging approaches are unable to assess this important paramete...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3720
更新日期:2017-07-01 00:00:00
abstract::A short-echo spectroscopic imaging sequence extended with a frequency-selective multiple-quantum- coherence technique (Sel-MQC) is presented. The method enables acquisition of a complete water-suppressed proton spectrum with a short echo time and filtering of the J-coupling metabolite, lactate, from co-resonant lipids...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1284
更新日期:2008-11-01 00:00:00
abstract::In vivo metabolic imaging using hyperpolarized [1-(13)C]pyruvate provides localized biochemical information and is particularly useful in detecting early disease changes, as well as monitoring disease progression and treatment response. However, a major limitation of hyperpolarized magnetization is its unrecoverable d...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3346
更新日期:2015-08-01 00:00:00
abstract::The leakage of metabolites from frozen and thawed tissue biopsies was measured semi-quantitatively by high-field (8.5 T) proton MRS. Human prostate and rat brain tissue specimens, frozen within 1 min of collection, lost significant and variable amounts of diagnostic metabolites immediately upon thawing. For prostate t...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.815
更新日期:2003-04-01 00:00:00
abstract::Brain activation studies in humans have shown the dynamic nature of neuronal N-acetylaspartate (NAA) and N-acetylaspartylglutamate (NAAG) based on changes in their MRS signals in response to stimulation. These studies demonstrated that upon visual stimulation there was a focal increase in cerebral blood flow (CBF) and...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3629
更新日期:2016-12-01 00:00:00
abstract::Quantitative analysis of extracts from two Ehrlich ascites tumour cell lines (Lettre cells) by 19F NMR in vitro demonstrated that one Lettre cell line (designated LLM) metabolized 30-50% less 5-fluorouracil to 5-fluoronucleotides when compared to the other cell line (designated LHM). HPLC analysis of these cellular ex...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940040605
更新日期:1991-12-01 00:00:00
abstract::The application of 31P MR spectroscopy in the characterization and treatment of malignant human extremity tumors is reviewed and placed in the perspective of results obtained in murine sarcomas. Despite the now widespread acquisition of gradient localized spectral maps, the low spatial resolution that can be achieved ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/(sici)1099-1492(1998110)11:7<341::aid-nbm5
更新日期:1998-11-01 00:00:00
abstract::The purpose of this study was to investigate the reliability of 3D isotropic MRI and quantitative multi-parametric MRI characterization on an orthotopic pancreatic head tumor model in rats. 3D isotropic T2 -weighted MRI was performed as a routine for tumor longitudinal follow-up and volume estimation. Common bile duct...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3676
更新日期:2017-02-01 00:00:00
abstract::Encoding of spatial information in magnetic resonance imaging is conventionally accomplished by using magnetic field gradients. During gradient encoding, the position in k-space is determined by a time-integral of the gradient field, resulting in a limitation in imaging speed due to either gradient power or secondary ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1399
更新日期:2009-11-01 00:00:00
abstract::The aim of this study was to acquire the transient MRI signal of hyperpolarized tracers and their metabolites efficiently, for which specialized imaging sequences are required. In this work, a multi-echo balanced steady-state free precession (me-bSSFP) sequence with Iterative Decomposition with Echo Asymmetry and Leas...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4291
更新日期:2020-06-01 00:00:00
abstract::This review assesses the progress of NMR in the field of hemorheology, i.e., the assessment of the mechanical and physical characteristics of flowing blood. The review deals with the intrinsic rheological properties of the red cell and its constituents (membrane, cytoplasm), which are dominant in the microcirculation,...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940020102
更新日期:1989-06-01 00:00:00
abstract::Early imaging or blood biomarkers of tumor response is needed to customize anti-tumor therapy on an individual basis. This study evaluates the sensitivity and relevance of five potential MRI biomarkers. Sixty nude rats were implanted with human glioma cells (U-87 MG) and randomized into three groups: one group receive...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1611
更新日期:2011-06-01 00:00:00
abstract::A novel polarizer based on the dissolution-dynamic nuclear polarization (DNP) method has been designed, built and tested. The polarizer differs from those previously described by being designed with sterile use intent and being compatible with clinical use. The main features are: (1) an integral, disposable fluid path...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1682
更新日期:2011-10-01 00:00:00
abstract::A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous 19F NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940050605
更新日期:1992-11-01 00:00:00
abstract::We have developed a reliable, reproducible model of hypoxia in the gerbil. 1H and 31P NMR spectroscopy demonstrates that cerebral energy metabolism is very resistant to hypoxia. Cerebral blood flow (measured by hydrogen clearance) began to increase when the arterial oxygen tension (paO2) was reduced to 40-50 mm Hg, an...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940050109
更新日期:1992-01-01 00:00:00
abstract::Signal of lung parenchymal tissue from the living rat and mouse lung was detected at 4.7 T with a good signal-to-noise ratio and motion-suppressed artifacts using a short TE gradient-echo sequence. Neither cardiac nor respiratory gating were applied, and animals respired freely during data collection. Mean T(2)* relax...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.706
更新日期:2001-08-01 00:00:00
abstract::In order to keep subscribers up-to-date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of NMR in biomedicine. Each bibliography is divided into 9 sections: 1 Books, Re...
journal_title:NMR in biomedicine
pub_type:
doi:10.1002/nbm.667
更新日期:2001-02-01 00:00:00
abstract::Recent work has shown that solid-state (1) H and (31) P MRI can provide detailed insight into bone matrix and mineral properties, thereby potentially enabling differentiation of osteoporosis from osteomalacia. However, (31) P MRI of bone mineral is hampered by unfavorable relaxation properties. Hence, accurate knowled...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.2930
更新日期:2013-09-01 00:00:00
abstract::A study was undertaken to determine if the vascular characteristics measured by dynamic contrast-enhanced magnetic resonance imaging (primarily permeability surface area product and extracellular-extravascular tissue volume fraction) would be beneficial in explaining the inter-lesion metabolic heterogeneity displayed ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.675
更新日期:2000-12-01 00:00:00
abstract::The emergence and rapid development of activatable contrast agents (CAs), whose relaxivity changes in response to the variation of a specific marker in the surrounding physiological microenvironment, have expanded the scope of MRI beyond anatomical and functional imaging to also convey information at the cellular and ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.2870
更新日期:2013-07-01 00:00:00
abstract::The transverse relaxation rates R2 and R2* were measured at 3 T in the substantia nigra of the midbrain of adult normal human controls. The relaxation rate arising from magnetic inhomogeneities, R2', was calculated from the relationship R2* = R2 + R2'. No significant differences were found for any parameters between l...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940080106
更新日期:1995-02-01 00:00:00
abstract::Boiling histotripsy (BH) is a new high intensity focused ultrasound (HIFU) ablation technique to mechanically fragmentize soft tissue into submicrometer fragments. So far, ultrasound has been used for BH treatment guidance and evaluation. The in vivo histopathological effects of this treatment are largely unknown. Her...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3520
更新日期:2016-06-01 00:00:00
abstract::Changes in physiological parameters such as cerebral blood flow, cerebral blood volume, oxygen extraction, and the size and distribution of cerebral blood vessels, result in changes in the local concentration of deoxyhaemoglobin ([dHb]). The purpose of this study was to quantitatively investigate the dependence of the...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199810)11:6<281::aid-nbm52
更新日期:1998-10-01 00:00:00
abstract::The processes of tumor invasion and metastasis have been well characterized at the molecular level, and numerous biomarkers of tumor aggressiveness have been discovered. Molecular imaging offers the opportunity to depict specific cell markers relevant to tumor aggressiveness. Here, we describe the role of MRI in ident...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1590
更新日期:2011-07-01 00:00:00
abstract::During magnetic resonance imaging (MRI) examinations, the average specific absorption rate (SAR) of the whole body is calculated as an index of global energy deposition in biological tissue without taking into account the presence of metallic implants or conductive materials. However, this global SAR calculation is no...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3849
更新日期:2018-01-01 00:00:00