Abstract:
:Quantitative analysis of extracts from two Ehrlich ascites tumour cell lines (Lettre cells) by 19F NMR in vitro demonstrated that one Lettre cell line (designated LLM) metabolized 30-50% less 5-fluorouracil to 5-fluoronucleotides when compared to the other cell line (designated LHM). HPLC analysis of these cellular extracts showed a significant decrease in the concentration of the cytotoxic nucleotide 5-fluorouridine triphosphate in LLM cells compared to LHM cells. No major differences could be observed in the 31P and 1H NMR spectra of the two cell lines. Growth inhibition studies in vitro demonstrated that LLM cells were less sensitive to 5-fluorouracil than LHM cells. These results are consistent with the hypothesis that 19F NMR visible levels of 5-fluoronucleotides can predict the cytotoxicity of the anti-cancer drug 5-fluorouracil.
journal_name
NMR Biomedjournal_title
NMR in biomedicineauthors
McSheehy PM,Maxwell RJ,Griffiths JRdoi
10.1002/nbm.1940040605subject
Has Abstractpub_date
1991-12-01 00:00:00pages
274-8issue
6eissn
0952-3480issn
1099-1492journal_volume
4pub_type
杂志文章abstract::Spin-exchange NMR techniques enable the measurement of the rates of exchange of solutes between chemically or physically distinct sites in reactions taking place at chemical equilibrium. The time scale of the events that are able to be investigated lies in the neighbourhood of 1 s. The earliest studies in this area of...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940030303
更新日期:1990-06-01 00:00:00
abstract::This paper describes the use of off-resonance saturation to further manipulate the blood oxygenation level dependent (BOLD) contrast of fMRI. A customized narrow bandwidth radiofrequency pulse, applied with a range of frequency offsets prior to selection of each slice, was designed and incorporated into a gradient ech...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/(sici)1099-1492(199706/08)10:4/5<208::aid-
更新日期:1997-06-01 00:00:00
abstract::A current limitation of MR spectroscopic imaging of multiple skeletal muscles is prolonged scan duration. A significant reduction in the total scan duration using the echo-planar correlated spectroscopic imaging (EP-COSI) sequence was accomplished using two bipolar readout trains with different phase-encoded echoes fo...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3171
更新日期:2014-10-01 00:00:00
abstract::The purpose of this study was to investigate the feasibility of interventional MRI-guided local agent delivery into pig common bile duct (CBD) walls using a newly designed MR-compatible, needle-integrated balloon catheter system. We first designed a needle-integrated balloon catheter system that comprised of a 22 G MR...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3308
更新日期:2015-06-01 00:00:00
abstract::In many studies, it has been demonstrated that (1)H MRSI of the human prostate has great potential to aid prostate cancer management, e.g. in the detection and localisation of cancer foci in the prostate or in the assessment of its aggressiveness. It is particularly powerful in combination with T2 -weighted MRI. Never...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.2973
更新日期:2014-01-01 00:00:00
abstract::Natural abundance proton-decoupled (13)C magnetic resonance spectroscopy was used to establish the in vivo lipid composition of normal adipose tissue and the corresponding effects of altered lipid diets. Experiments were performed on a standard 1.5 T clinical MR scanner using a double-tuned (1)H-(13)C coil. Peaks from...
journal_title:NMR in biomedicine
pub_type: 临床试验,杂志文章
doi:10.1002/nbm.824
更新日期:2003-05-01 00:00:00
abstract::Normal subjects performed voluntary, isometric exercise 1 s contraction, 1 s rest for 10 min) of the first dorsal interosseous (FDI) muscle with a target force of 25, 50 and 100% of the maximal voluntary contraction (MVC) force. 31P NMR spectra were collected continuously before, during and after exercise. Data were a...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940030504
更新日期:1990-10-01 00:00:00
abstract::By combining intravoxel incoherent motion (IVIM) and diffusion tensor imaging (DTI) we introduce a new diffusion model called intravoxel oriented flow (IVOF) that accounts for anisotropy of diffusion and the flow-related signal. An IVOF model using a simplified apparent flow fraction tensor (IVOFf ) is applied to diff...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3584
更新日期:2016-10-01 00:00:00
abstract::One of the major goals of hyperpolarized-gas MR imaging has been to obtain (129)Xe dissolved-phase images in humans. Since the dissolved-phase signal is much weaker than the gas-phase signal, highly optimized MR pulse sequences are required to obtain adequate images during a single breath-hold. In particular, a solid ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/1099-1492(200006)13:4<220::aid-nbm638>3.0.
更新日期:2000-06-01 00:00:00
abstract::Renal urea handling is central to the urine concentrating mechanism, and as such the ability to image urea transport in the kidney is an important potential imaging biomarker for renal functional assessment. Glucagon levels associated with changes in dietary protein intake have been shown to influence renal urea handl...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4028
更新日期:2019-01-01 00:00:00
abstract::The cerebral blood volume (CBV) is a crucial physiological indicator of tissue viability and vascular reactivity. Thus, noninvasive CBV mapping has been of great interest. For this, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles, including monocrystalline iron oxide nanoparticles, can be used as long-ha...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.2885
更新日期:2013-08-01 00:00:00
abstract::The aim of this study was to measure choline/creatine (Ch/Cr) levels through (1)H-MRS and apparent diffusion coefficient (ADC) values through diffusion-weighted MRI, and to correlate these values with the prognostic parameters of head and neck squamous cell carcinoma (HNSCC). The institutional review board approved th...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3472
更新日期:2016-04-01 00:00:00
abstract::Increased pulmonary lactate production is correlated with severity of lung injury and outcome in acute respiratory distress syndrome (ARDS) patients. This study was conducted to investigate the relative contributions of inflammation and hypoxia to the lung's metabolic shift to glycolysis in an experimental animal mode...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4380
更新日期:2020-11-01 00:00:00
abstract::A quantitative magnetization transfer (qMT) technique was employed to quantify the ratio of the sizes of the bound and free water proton pools in ex vivo mouse brains. The goal was to determine the pool size ratio sensitivity to myelin. Fixed brains from both shiverer mice and control littermates were imaged. The pool...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1358
更新日期:2009-06-01 00:00:00
abstract::γ-Aminobutyric acid (GABA) and glutamate (Glu), major neurotransmitters in the brain, are recycled through glutamine (Gln). All three metabolites can be measured by magnetic resonance spectroscopy in vivo, although GABA measurement at 3 T requires an extra editing acquisition, such as Mescher-Garwood point-resolved sp...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3847
更新日期:2018-01-01 00:00:00
abstract::Spontaneous fluctuations in the blood oxygenation level-dependent (BOLD) MRI signal during the resting state are increasingly being studied in healthy and diseased brain in humans and animal models. Yet, the relationship between functional brain status and the characteristics of spontaneous BOLD fluctuations remains p...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1556
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVES:Quantitative MRI (qMRI) of muscles is a promising tool to measure disease progression or to assess therapeutic effects in neuromuscular diseases. Longitudinal imaging studies are needed to show sensitivity of qMRI in detecting disease progression in spinal muscular atrophy (SMA). In this pilot study we there...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4473
更新日期:2021-01-22 00:00:00
abstract::The availability of high-field-strength magnetic resonance imaging (MRI) systems has brought about the development of techniques that aim to map myelination via the exploitation of various contrast mechanisms. Myelin mapping techniques have the potential to provide tools for the diagnosis and treatment of diseases, su...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3877
更新日期:2018-03-01 00:00:00
abstract::Chronic pain is a major problem for the individual and for society. Despite a range of drugs being available to treat chronic pain, only inadequate pain relief can be achieved for many patients. There is therefore a need for the development of new analgesic compounds. The assessment of pain depends to date entirely on...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1076
更新日期:2006-10-01 00:00:00
abstract::The steady-state distribution of the general anesthetic halothane in different rat tissues, including a renal adenocarcinoma with and without hyperthermia treatment, has been evaluated by in vivo 19F NMR spectroscopy. The 19F spectra of halothane (which is a hydrophobic probe) from within tissue show differences in th...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940060502
更新日期:1993-09-01 00:00:00
abstract::The purpose of this work was to harmonize data acquisition and post-processing of single voxel proton MRS ((1) H-MRS) at 7 T, and to determine metabolite concentrations and the accuracy and reproducibility of metabolite levels in the adult human brain. This study was performed in compliance with local institutional hu...
journal_title:NMR in biomedicine
pub_type: 杂志文章,多中心研究
doi:10.1002/nbm.3252
更新日期:2015-03-01 00:00:00
abstract::We show how quantitative information about proton handling in human skeletal muscle in exercise and recovery can be obtained by 31P MRS and illustrate this with data from metabolic disorders. Proton production, proton efflux and passive buffering can be distinguished by comparing changes in [phosphocreatine] and pH at...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1940060112
更新日期:1993-01-01 00:00:00
abstract::Balanced steady-state free precession imaging has recently been suggested for chemical exchange detection (bSSFPX). The objective of this work is to investigate the contributions of microstructural, chemical shift and chemical exchange effects to the asymmetry of the bSSFP profile at field strengths of 3 T and 9.4 T. ...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.4200
更新日期:2020-03-01 00:00:00
abstract::During the measurement of hyperpolarized 129 Xe magnetic resonance imaging (MRI), the diffusion-weighted imaging (DWI) technique provides valuable information for the assessment of lung morphometry at the alveolar level, whereas the chemical shift saturation recovery (CSSR) technique can evaluate the gas exchange func...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3730
更新日期:2017-08-01 00:00:00
abstract::We review several methods that have been developed to infer microstructural and physiological information about isotropic and anisotropic tissues from diffusion weighted images (DWIs). These include Diffusion Imaging (DI), Diffusion Tensor Imaging (DTI), isotropically weighted imaging, and q-space imaging. Just as DI ...
journal_title:NMR in biomedicine
pub_type: 杂志文章,评审
doi:10.1002/nbm.1940080707
更新日期:1995-11-01 00:00:00
abstract::A novel polarizer based on the dissolution-dynamic nuclear polarization (DNP) method has been designed, built and tested. The polarizer differs from those previously described by being designed with sterile use intent and being compatible with clinical use. The main features are: (1) an integral, disposable fluid path...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1682
更新日期:2011-10-01 00:00:00
abstract::The leakage of metabolites from frozen and thawed tissue biopsies was measured semi-quantitatively by high-field (8.5 T) proton MRS. Human prostate and rat brain tissue specimens, frozen within 1 min of collection, lost significant and variable amounts of diagnostic metabolites immediately upon thawing. For prostate t...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.815
更新日期:2003-04-01 00:00:00
abstract::This paper compares two spectral processing methods for obtaining quantitative measures from in vivo prostate spectra, evaluates their effectiveness, and discusses the necessary modifications for accurate results. A frequency domain analysis (FDA) method based on peak integration was compared with a time domain fittin...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1008
更新日期:2006-04-01 00:00:00
abstract::MRS provides a valuable tool for the non-invasive detection of brain γ-aminobutyric acid (GABA) in vivo. GABAergic dysfunction has been observed in the aging cerebellum. The study of cerebellar GABA changes is of considerable interest in understanding certain age-related motor disorders. However, little is known about...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.3381
更新日期:2015-10-01 00:00:00
abstract::The aim of this work was to identify spectral markers of cell proliferation that could be of use in clinical MRS. Cultured C6 ATCC rat glioma cells were used as models for this purpose and metabolites were extracted with perchloric acid at three different growth curve stages: log, confluence and post-confluence. 1D an...
journal_title:NMR in biomedicine
pub_type: 杂志文章
doi:10.1002/nbm.1024
更新日期:2006-04-01 00:00:00