Abstract：
:The purpose of this study was to estimate clinical validity of a new available immunoradiometric assay for circulating intact human BGP (N-tact Osteo SP) by measuring this protein in a large number of normal subjects and patients with the most common metabolic bone diseases. One hundred normal subjects were studied in order to obtain our normal ranges (4.9 +/- 1.7 ng/ml). The mean values found in 28 patients with primary hyperparathyroidism (17.5 +/- 22.8 ng/ml, P < 0.001), 15 glucocorticoid-treated patients (1.9 +/- 1.5, P < 0. 001), 10 patients with hypoparathyroidism (1.5 +/- 0.7, P < 0.001), 9 with hyperthyroidism (8.3 +/- 3.8, P < 0.001), 8 with skeletal metastases (7.2 +/- 2.3, P < 0.001), and 4 with humoral hypercalcemia of malignancy (2.42 +/- 1.91, P < 0.005) were significantly different from mean values found in normal subjects. Mean decrease of serum osteocalcin T-score values was significantly greater when evaluated by N-tact Osteo SP assay in 15 steroid-treated patients (-1.4 +/- 1.0) and 19 primary hyperparathyroid (PHPT) patients (3.6 +/- 1.9), compared with the mean values obtained with the Elsa-Osteo assay (-0.67 +/- 1.2, P < 0. 002 and 4.3 +/- 2.8, P < 0.04, respectively). We found significant correlations between the global skeletal uptake of 99mTc-methylendiphosphonate and serum BGP levels assayed by both N-tact Osteo SP (P < 0.01) and Elsa-Ost-Nat assay (P < 0.05). Our results indicate that this new immunoradiometric assay for the intact human osteocalcin has the potential for good discrimination between normal subjects and patients with both low and high bone turnover. Furthermore, our findings emphasize the fact that, in the absence of available standardized commercial assays, one should rely on only one assay because different results are obtained by different assays under different clinical conditions.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Minisola S,Pacitti MT,Romagnoli E,Rosso R,Carnevale V,Caravella P,Scillitani A,Dicembrino Fdoi
10.1007/pl00005815subject
Has Abstractpub_date
1999-05-01 00:00:00pages
365-9issue
5eissn
0171-967Xissn
1432-0827journal_volume
64pub_type
杂志文章abstract：:High PTH levels increase bone turnover and decrease bone mineral density (BMD). Low plasma 25-hydroxyvitamin D (25OHD) levels cause secondary hyperparathyroidism, but the relative contribution of low 25OHD and high PTH levels on risk of fracture is largely unknown. Within the cohort of women (n = 2,016) included in th...
journal_title：Calcified tissue international
pub_type： 杂志文章,多中心研究
doi：10.1007/s00223-010-9454-0
更新日期：2011-03-01 00:00:00
abstract：:Melorheostosis is an exceptionally rare sclerosing hyperostosis that typically affects the appendicular skeleton in a limited segmental fashion. It occasionally occurs on a background of another benign generalised sclerosing bone condition, known as osteopoikilosis caused by germline mutations in LEMD3, encoding the i...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-019-00543-y
更新日期：2019-05-01 00:00:00
abstract：:Healing trabecular microfractures are a common feature in cancellous bone. These lesions, when observed in macerated cancellous bone slices, measure about 500 microns in diameter and surround fractures in trabeculae with microcallus. Whether microcallus is a structure acting primarily as a transient brace, preventing ...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/BF01673424
更新日期：1993-01-01 00:00:00
abstract：:High impact loading is known to prevent some of the age-related bone loss but its effects on the density distribution of cortical bone are relatively unknown. This study examined the effects of age and habitual sprinting on tibial and fibular mid-shaft bone traits (structural, cortical radial and polar bone mineral de...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-014-9881-4
更新日期：2014-08-01 00:00:00
abstract：:Dual photon absorptiometry (DPA) is currently the most widely used method for noninvasive bone mineral density (BMD) measurement of the axial skeleton. Dual energy X-ray absorptiometry (DEXA) is a recently developed technique that uses an X-ray tube as a photon source; it has demonstrated several significant advantage...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556250
更新日期：1991-11-01 00:00:00
abstract：:Regular tennis play is associated with impressive asymmetries in bone strength in favor of the racquet arm, particularly in the humerus. However, the relative effects of service and ground strokes are not known. Serendipitously, we encountered a 46-year-old regular tennis player who has played service and ground strok...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-015-9995-3
更新日期：2015-08-01 00:00:00
abstract：:This study sought to develop a noninvasive, reliable, clinically relevant, and easy-to-implement mouse model that can be used for investigation of the pathophysiology of PTOA and for preclinical testing of new therapies of PTOA. Accordingly, we have established a closed intraarticular tibial plateau compression loadin...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00614-0
更新日期：2020-02-01 00:00:00
abstract：:The effect of teriparatide treatment on microdamage accumulation has yet to be examined in animal studies. The purpose of this study was to investigate the effect of once-weekly teriparatide treatment on bone microdamage accumulation and the relationship between microdamage parameters and bone mass, architecture, turn...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0500-7
更新日期：2019-04-01 00:00:00
abstract：:Enamel of intact human teeth laser irradiated in vitro under certain conditions is known to have less subsurface demineralization than unirradiated enamel on exposure to acid; consequently, the potential use of laser irradiance to reduce caries is apparent. The laser-induced physical and/or chemical changes that cause...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/BF02556711
更新日期：1986-04-01 00:00:00
abstract：:The discriminating ability and relevance of clinical risk factors, quantitative ultrasound (QUS) variables, X-ray-based bone mineral density (BMD) and hip axis length (HAL) measurements to evaluate the risk of osteoporotic fracture in elderly Brazilian women were examined in this study. QUS at the calcaneus (Achilles ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-002-1096-4
更新日期：2003-12-01 00:00:00
abstract：:Plasma membrane vesicles were prepared from chicken osteoclasts, and active calcium transport was demonstrated in a spectrofluorimetric assay using the fluorescent calcium concentration indicator, fura-2. Transport activity was inhibited by quercetin (10 microM), sodium vanadate (10 microM), and the anticalmodulin age...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00334493
更新日期：1992-10-01 00:00:00
abstract：:The effect of parathormone (PTH), lipopolysaccharide (LPS), or interleukin-1 (IL-1) on calcium release and collagen degradation in bone was examined in vitro using labeled neonatal calvaria of normal mice and also of osteopetrotic microphthalmic (mi/mi) mice that have defective osteoclasts. All three agents stimulated...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02554878
更新日期：1985-05-01 00:00:00
abstract：:Raf-1, the product of proto-oncogene c-raf-1, has key roles in the signal transduction pathways within the cell. The molecular mechanisms of tooth development in the mouse embryo are not known in detail. We examined the expression of Raf-1 during subsequent tooth development by immunohistochemical analysis. In mouse e...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02509547
更新日期：1996-01-01 00:00:00
abstract：:Our knowledge of the developmental changes in the concentration of serum and bone osteocalcin (OC) is limited. To investigate the interrelationship between skeletal and circulatory OC during acquisition of peak bone density in mice, we examined the temporal changes in the concentration of serum and bone OC from 3 to 1...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-1106-y
更新日期：2002-11-01 00:00:00
abstract：:Previous studies have indicated that children with inflammatory bowel disease (IBD) may not achieve optimal bone mass. We evaluated the skeletal characteristics in children and adolescents with IBD. This cross-sectional cohort study comprised 80 IBD patients (median age 14.9 years, range 5-20) with a median disease du...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-012-9617-2
更新日期：2012-08-01 00:00:00
abstract：:The tumor-suppressor p53 is a transcription factor that regulates a number of genes in the process of cell-cycle inhibition, apoptosis, and DNA damage. Recent studies have revealed a crucial role for p53 in bone remodeling. In our previous studies we have shown that p53 is an important regulator of osteoblast differen...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9533-x
更新日期：2011-11-01 00:00:00
abstract：:The fossil record shows that bone remodeling has existed since the earliest large vertebrates became weight-bearing on land, but the functions of remodeling have long been debated. The principal protagonists in this debate have been those favoring a mechanical function and those asserting that remodeling serves to mov...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-002-1059-9
更新日期：2003-08-01 00:00:00
abstract：:To experimentally examine the participation of amelogenins in controlled mineral-phase maturation of mammalian enamel, the identification of the individual proteins and their corresponding gene(s) is required. For this purpose, cDNAs were constructed from polyadenylated RNA from 2-day postnatal murine teeth, molecular...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556627
更新日期：1987-04-01 00:00:00
abstract：:The influence of body mass index (BMI) on T scores for total body bone mineral content (TBBMC) and regional bone mineral content (RBMC) was studied in 186 healthy women: 100 postmenopausal, 35 perimenopausal, and 51 premenopausal. The three groups were divided by BMI >25 kg/m2 and BMI <25 kg/m2 and the postmenopausal ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900310
更新日期：1997-08-01 00:00:00
abstract：:Abnormal PTH secretion and cell growth in hyperparathyroid tissues are accompanied with reduced expression of Ca2+-sensing receptor (CaR) which plays a key role in Ca-regulated PTH release. In this study, we examined the receptor expression in parathyroid adenomas using specific anti-CaR antibody and investigated rela...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900617
更新日期：1999-03-01 00:00:00
abstract：:Alkaline phosphatase is detected in both soluble and particulate fractions of rat bone. The concentration of alkaline phosphatase in the particulate fraction of rat skull cap bone and femur is high during the peak period of calcification of these bones suggesting the possibility of using it as a marker for the rate of...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555116
更新日期：1986-09-01 00:00:00
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journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230001168
更新日期：2001-03-01 00:00:00
abstract：:The bone mineral status of 17 girls with Turner's syndrome was evaluated by single photon absorptiometry. Bone mineral content (BMC) was 25.4% below that predicted by normalization for age, sex, height, weight, and bone width. Only 25% of this demineralization could be attributed to delayed skeletal maturation. Bones ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411296
更新日期：1982-01-01 00:00:00
abstract：:We describe a tall-statured 14-year-old boy who illustrated the full phenotypic and radiographic features of Stickler syndrome type I. A bone biopsy showed evidence of reduced bone mass and bone turnover, such as reduced BV/TV (-43%), TbTh (-29%), and OS/BS (-48%), Ob.S/BS (-27%), and Oc/BS (-47%) compared to "age-mat...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9324-9
更新日期：2010-02-01 00:00:00
abstract：:Studies suggest that long-term use of proton pump inhibitors (PPIs) may be associated with an increased risk of fracture. However, the role of medication adherence in this association is not fully understood. A retrospective cohort study was conducted to examine the relationship between PPI use/adherence and fracture ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-014-9855-6
更新日期：2014-06-01 00:00:00
abstract：:The goals of internal fixation are an accurate reduction and stable fixation in the presence of adequate bony vascularity. This can be achieved by a variety of means including plate fixation. A certain amount of periosteal stripping is necessary for proper open reduction of a fracture and for proper plate application....
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900080
更新日期：1996-07-01 00:00:00
abstract：:This cross-sectional, observational study was designed to identify clinical risk factors of osteoporosis and fractures in Korean women to validate the probability of osteoporosis and subsequent fractures. A total of 1541 Korean women were recruited nationally. Fracture history of any site, risk factors of osteoporosis...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9182-x
更新日期：2008-12-01 00:00:00
abstract：:Identifying persons with a high risk of osteoporotic fractures remains a challenge. DXA uptake in women with elevated risk of osteoporosis seems to be depending on distance to scanning facilities. This study aimed to investigate the ability of a small portable scanner in identifying women with reduced bone mineral den...
journal_title：Calcified tissue international
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00223-015-0084-4
更新日期：2016-03-01 00:00:00
abstract：:Sarcopenia and osteoporosis are two sides of the same coin. They represent different aspects of the same age-related process of musculoskeletal atrophy and together culminate in falls, fractures, deconditioning, and increased mortality in older individuals. However, the current therapeutic approach to the prevention o...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-015-9956-x
更新日期：2015-03-01 00:00:00
abstract：:The effects of fluoride on human enamel and selachian enameloid in vitro were visualized in TEM and analyzed with electron diffraction. It is demonstrated that under precise pH conditions, inducing concentration balance between F-ions and apatite, calcium fluoride is no longer formed, and crystalline changes occur ins...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02409406
更新日期：1981-01-01 00:00:00
