Abstract：
:To experimentally examine the participation of amelogenins in controlled mineral-phase maturation of mammalian enamel, the identification of the individual proteins and their corresponding gene(s) is required. For this purpose, cDNAs were constructed from polyadenylated RNA from 2-day postnatal murine teeth, molecularly cloned into lambda-gt11 expression vectors and transfected into E. coli. The cDNA library was screened for amelogenin gene(s) by using either antibody or nucleic acid probes. An amelogenin cDNA clone encoding 79 carboxy-terminal amino acid residues and 100 nucleotides of the 3' noncoding sequence was demonstrated to contain a major antigenic site for amelogenin protein by immunostaining of specific amelogenin proteins from total extracted enamel protein blots using clonal epitope selected antibody. This is the first report linking amelogenin epitope(s) to a defined DNA sequence, and consequently a defined portion of the amino acid sequence for amelogenins. Secondary structure analysis, based on the relative average linear hydropathy of the amino acid sequence of amelogenin, predicted epitopes in the amino terminus of the molecule rather than the carboxy terminus. Our present data suggest that the carboxy terminus of the amelogenins is sufficiently externalized to be an antigenic domain. These data may be useful in subsequent structural analysis of amelogenin proteins and enhancing our understanding of their physicochemical participation in biomineralization.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Lau EC,Bessem CC,Slavkin HC,Zeichner-David M,Snead MLdoi
10.1007/BF02556627subject
Has Abstractpub_date
1987-04-01 00:00:00pages
231-7issue
4eissn
0171-967Xissn
1432-0827journal_volume
40pub_type
杂志文章abstract：:Primary cilia are responsible for sensing mechanical loading in osteocytes. However, the underlying working mechanism of cilia remains elusive. An osteocyte model is necessary to reveal the role of cilia. Furthermore, the osteocyte model should be with upregulated or downregulated primary cilium expression. Herein, we...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00756-6
更新日期：2020-12-01 00:00:00
abstract：:There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900111
更新日期：1996-09-01 00:00:00
abstract：:Gallium nitrate (GaN) reduces cancer-related hypercalcemia and inhibits bone resorption in vitro. This study investigated the effects of chronic GaN administration on bone, kidney, and parathyroid gland activity of growing rats. Experimental animals received GaN (1.75 mg elemental gallium i.p. QOD X 8, Ga+), and contr...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555260
更新日期：1987-05-01 00:00:00
abstract：:Matrix extracellular phosphoglycoprotein (MEPE) is an inhibitor of mineralization in situ and in cell cultures where altered expression is associated with oncogenic osteomalacia and hypophosphatemic rickets. The purpose of this study was to determine whether the intact protein or the peptide(s) originating from this p...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9313-z
更新日期：2010-01-01 00:00:00
abstract：:Parathyroid Hormone (PTH) stimulates calcium entry into bone cells in vitro, and this effect has been proposed to mediate the actions of PTH on bone. Using cells harvested from infant rat calvaria by collagenase digestion, we evaluated the sensitivity and specificity of this phenomenon. Calcium uptake was temperature ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02408543
更新日期：1980-01-01 00:00:00
abstract：:Previous observational studies have identified various risk factors associated with the development of osteoporosis, including sex hormone-binding globulin (SHBG). The aim of this study was to determine the potential causal effects of circulating SHBG concentrations on bone mineral density (BMD). Two-sample Mendelian ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00770-8
更新日期：2020-10-17 00:00:00
abstract：:In order to study in detail the processes leading to the resorption and ankylosis of teeth after trauma, the effects of cold application on the periodontal tissues were studied in the mouse. Liquid nitrogen was applied locally to the outer surface of the lower jaw which resulted in a freezing of the incisor and its su...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555735
更新日期：1986-07-01 00:00:00
abstract：:Histomorphometric evaluation of bone formation in an intravital tetracycline-labeled skeleton of a suddenly deceased osteoporotic woman is presented. The 24 skeletal sites investigated displayed substantial regional variations which, for two out of four cortical bone dynamics and all trabecular bone dynamics, were sig...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556619
更新日期：1987-04-01 00:00:00
abstract：:The aims of this study were to compare the response to therapy in disease activity in pagetic patients with and without skull involvement and the usefulness of bone markers in the evaluation of these patients. Forty patients with Paget's disease treated with tiludronate and 26 healthy controls were included. Serum tot...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0247-9
更新日期：2006-07-01 00:00:00
abstract：:Mononuclear phagocytes have been implicated as important cellular elements in the process of bone resorption. We have postulated that the recruitment and migration of mononuclear phagocytes to bone occurs via a mechanism(s) in which bone-derived chemotactic factors (BDCF) are released from foci undergoing resorption. ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557681
更新日期：1985-01-01 00:00:00
abstract：:The effect of long-chain n-3 polyunsaturated fatty acid (PUFA) on bone measurements was evaluated in gonad-intact middle-aged male rats. Seven rats were killed on day 0 of dietary intervention to determine bone parameters at baseline. Experimental rats (7/group) were fed one of the following lipid treatments (g/kg die...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9010-8
更新日期：2007-04-01 00:00:00
abstract：:Hepatitis C treatment with interferon alpha-2b (IFN-alpha) and ribavirin has been related to decreased bone mineral density. The aim of this study was to investigate the in vitro effects of different concentrations of ribavirin and IFN-alpha on osteoblast-like cells. Human osteoblast-like cells obtained by the outgrow...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-004-0175-0
更新日期：2004-08-01 00:00:00
abstract：:The aim of this study was to investigate the effect of transplanted chondrocytes on endochondral bone formation in cartilage repair tissue. In the knee joint of miniature pigs, cartilage lesions were treated by microfracturing and were then either left empty, covered with a collagen membrane, or treated by matrix-asso...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9288-9
更新日期：2009-11-01 00:00:00
abstract：:We compared the effects of four vitamin D metabolites, 1 alpha,25 dihydroxy vitamin D3 (1 alpha,25(OH)2D3), 1 alpha hydroxy vitamin D3 (1 alpha OH D3), 25 hydroxy vitamin D3 (25 OH D3), and 24R,25 dihydroxy vitamin D (24R,25(OH)sD3) on resorption and collagen synthesis in fetal rat bone maintained in organ culture. Re...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02408532
更新日期：1980-01-01 00:00:00
abstract：:We studied the acute phase response, including specific cytokine production, [interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor alpha(TNF alpha)] following a single dose of Aredia (disodium pamidronate) in patients with increased bone turnover and, in vitro, the role played by specific cytokines in the...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/s002239900353
更新日期：1997-11-01 00:00:00
abstract：:Differences in trabecular and cortical bone loss have been demonstrated clinically, but differences in bone loss at different skeletal sites remain unclear. We examined regional variations in bone loss histomorphometrically in two strains of mice in which osteopenia progresses spontaneously: tiptoe-walking Yoshimura (...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900455
更新日期：1998-05-01 00:00:00
abstract：:The phorbol ester 12-O-tetradecanoyl-13-acetate (TPA) blocked the growth of, and induced the appearance of processes in the human osteosarcoma cell line U-2 OS. The phorbol ester decreased the intracellular level of alkaline phosphatase (APase) activity (as measured per mg cell protein) and caused a marked increase in...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00582169
更新日期：1992-06-01 00:00:00
abstract：:The effect of parathormone (PTH), lipopolysaccharide (LPS), or interleukin-1 (IL-1) on calcium release and collagen degradation in bone was examined in vitro using labeled neonatal calvaria of normal mice and also of osteopetrotic microphthalmic (mi/mi) mice that have defective osteoclasts. All three agents stimulated...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02554878
更新日期：1985-05-01 00:00:00
abstract：:Limited information is available on the effect of bisphosphonates in men receiving corticosteroid therapy. We studied 184 men among the patients enrolled in two, double-blind, placebo-controlled, 1-year studies with similar protocols. The studies evaluated the effects of risedronate in patients beginning corticosteroi...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00223-001-1060-8
更新日期：2001-10-01 00:00:00
abstract：:Long chain polyunsaturated fatty acids (LCPUFAs) are involved in the regulation of bone metabolism. Increased dietary consumption of n-3, and possibly some n-6, LCPUFAs may limit postmenopausal bone loss. The aim of this study was to determine the effects on bone of specific fatty acids within the n-3 and n-6 LCPUFA f...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9080-7
更新日期：2007-12-01 00:00:00
abstract：:To study the risk of fractures associated with anxiolytics, sedatives, and hypnotics, we conducted a case-control study. Cases were all subjects with any fracture during the year 2000 (n = 124,655). For each case, three controls (n = 373,962) matched on age and gender were randomly drawn from the background population...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9095-0
更新日期：2008-01-01 00:00:00
abstract：:The binding of prostaglandin E2 (PGE2) to bone cells was studied to provide direct evidence for the existence of specific receptors in bone. Bone cells were isolated by collagenase digestion of fetal and newborn rat calvaria. Isolated cells were incubated with 3H-PGE2 and collected on Millipore filters. Specific bindi...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405038
更新日期：1983-01-01 00:00:00
abstract：:Bone loss occurs as early as the third decade and its cumulative effect throughout adulthood may impact risk for osteoporosis in later life, however, the genes and environmental factors influencing early bone loss are largely unknown. We investigated the role of genes in the change in bone mineral density (BMD) in par...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9197-3
更新日期：2009-02-01 00:00:00
abstract：:Urinary pyridinoline (pyr) and deoxypyridinoline (dpyr) are new markers for bone resorption, and serum osteocalcin reportedly indicates osteoblastic activity. Recently, a new ultrasound bone densitometer instrument has been developed that measures ultrasonic properties of the os calcis, namely, the speed of sound (SOS...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章
doi：10.1007/BF00298555
更新日期：1994-12-01 00:00:00
abstract：:The developing mandibular condylar growth center consists of a number of histologically distinct cell types. There is an increase in cell volume that takes place from the condylar surface layer through the center of ossification, resulting in a disorganized, irregular cellular pattern. Consequently, the isolation and ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00298747
更新日期：1995-01-01 00:00:00
abstract：:Sulfotransferase 1E1 (SULT1E1) catalyze estrogen into sulfate conjugation and is involved in the metabolism of phytoestrogen. A community-based cross-sectional study was conducted on 397 Korean women, to evaluate the association between genetic polymorphisms of SULT1E1 and bone mineral density (BMD) and the combined e...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-006-0008-4
更新日期：2006-09-01 00:00:00
abstract：:Adiponectin, a protein classically known to be secreted by adipocytes, is also secreted by bone-forming cells. Results of previous studies have been contradictory as to whether serum adiponectin and bone mineral density (BMD) are associated. The aim of this study was to investigate a possible association between serum...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9376-x
更新日期：2010-07-01 00:00:00
abstract：:Postmenopausal osteoporosis induced by estrogen deficiency causes inadequate new bone formation and affects millions of women worldwide. Melatonin can improve bone mineral density at the femoral neck in postmenopausal women with osteopenia. This study aimed to investigate the mechanism of melatonin in estrogen deficie...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0428-y
更新日期：2018-10-01 00:00:00
abstract：:Using data from the Peking Vertebral Fracture Study, we conducted a longitudinal cohort study to investigate the association between type 2 diabetes mellitus (T2DM) and the risk of incident fractures, especially of vertebral fractures (VFs), and we also examined the modifying effect of body mass index (BMI) on this as...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00598-x
更新日期：2019-11-01 00:00:00
abstract：:The objective of this study was to compare the bone mineral density (BMD) of men with Down syndrome (DS) to otherwise mentally retarded (MR) men and to investigate whether leg muscle strength of these patients is related to BMD. Two groups with MR (with and without DS) participated in the study, having met the followi...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230010035
更新日期：2000-03-01 00:00:00
