Abstract:
:Primary cilia are responsible for sensing mechanical loading in osteocytes. However, the underlying working mechanism of cilia remains elusive. An osteocyte model is necessary to reveal the role of cilia. Furthermore, the osteocyte model should be with upregulated or downregulated primary cilium expression. Herein, we used a pharmacological method to regulate the cilium formation of osteocytes. After screening, some pharmacological agents can regulate the cilium formation of osteocytes. We performed a CCK-8 assay to analyze the optimal working conditions of the drugs for MLO-Y4 cells. The agents include chloral hydrate (CH), Gd3+, Li+, and rapamycin. The expression of cilia affects the cellular functions, including mechanosensitivity, of osteocytes. Results showed that CH downregulated the cilium formation and ciliogenesis of osteocytes. In addition, Gd3+, Li+, and rapamycin upregulated the cilium expression of osteocytes. Moreover, the cilium expression positively correlated with the mechanosensitivity of osteocytes. This work reveals the role of primary cilia in the mechanosensing of osteocytes.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Ding D,Yang X,Luan HQ,Wu XT,He C,Sun LW,Fan YBdoi
10.1007/s00223-020-00756-6subject
Has Abstractpub_date
2020-12-01 00:00:00pages
625-635issue
6eissn
0171-967Xissn
1432-0827pii
10.1007/s00223-020-00756-6journal_volume
107pub_type
杂志文章abstract::Molybdenum (Mo), at high concentrations, induces changes in the epiphyseal growth plate through its effects on copper (Cu) metabolism but it is unclear whether or not Mo can induce changes independent of its effects on copper status. To this end, the effect of Mo on longitudinal bone growth was examined in rats. Dieta...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF01321835
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF00298499
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journal_title:Calcified tissue international
pub_type: 杂志文章,随机对照试验
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更新日期:2011-05-01 00:00:00
abstract::Newly developed fuzzy logic-derived structural parameters were used to characterize trabecular bone architecture in high-resolution magnetic resonance imaging (HR-MRI) of human cadaver calcaneus specimens. These parameters were compared to standard histomorphological structural measures and analyzed concerning perform...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-007-9058-5
更新日期:2007-10-01 00:00:00
abstract::Roux-en-Y gastric bypass surgery (RYGB) is an effective treatment of morbid obesity, with positive effects on obesity-related complications. The treatment is associated with bone loss, which in turn might increase fracture risk. The aim of this study was to evaluate changes in bone mineral density (BMD) and bone archi...
journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-015-0091-5
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s00223-017-0360-6
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/s002239900512
更新日期:1998-09-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002239900258
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02409507
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/s002230010068
更新日期:2000-05-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
doi:10.1007/s00223-015-0022-5
更新日期:2016-04-01 00:00:00
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF02555965
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journal_title:Calcified tissue international
pub_type: 杂志文章
doi:10.1007/BF01352006
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章,评审
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
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