Abstract：
:The binding of prostaglandin E2 (PGE2) to bone cells was studied to provide direct evidence for the existence of specific receptors in bone. Bone cells were isolated by collagenase digestion of fetal and newborn rat calvaria. Isolated cells were incubated with 3H-PGE2 and collected on Millipore filters. Specific binding was determined by subtracting the binding that occurred with 10(-6) M non-radioactive PGE2 and 3H-PGE2 from that with 3H-PGE2 alone. With heterogeneous cell preparations and at PGE2 concentrations from 10(-9) - 1.7 X 10(-8) M at 37 degrees C, specific binding reached steady state within 10 min. Bound 3H-PGE2 was displaced by the addition of increasing amounts of unlabeled PGE2. Inhibition of PGE2 binding was observed with PGE1 and the endoperoxide analog, U44069, but not with PGF2 alpha, a lipopolysaccharide, or 13,14-dihydro 15-keto PGE2. Studies with bone cell populations, obtained by sequential digestions, indicated that an osteoclastic population binds 30-fold more PGE2 than osteoblastic cells. Scatchard analyses revealed that the osteoclastic cells have an affinity constant for PGE2 binding similar to that obtained with heterogeneous populations. However, the PGE2 binding capacity in this osteoclastic population was fivefold greater than in the heterogeneous population. The osteoclastic population responded with an increase in cyclic AMP to lower concentrations of PGE2 than the osteoblastic populations. These studies suggest that differences in the binding capacity of PGE2 receptors exist among bone cell-types and that these differences are reflected in the cellular cyclic AMP response.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Dziak RM,Hurd D,Miyasaki KT,Brown M,Weinfeld N,Hausmann Edoi
10.1007/BF02405038subject
Has Abstractpub_date
1983-01-01 00:00:00pages
243-9issue
2eissn
0171-967Xissn
1432-0827journal_volume
35pub_type
杂志文章abstract：:Whole enamel scrapings from unerupted teeth of embryonic calves have been separated into fractions of varying density by stepwise centrifugation in bromoform-toluene mixtures of increasing specific gravity. Partition of enamel in this manner yields individual fractions of increasing mineral phase age and maturation. W...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02405006
更新日期：1983-01-01 00:00:00
abstract：:Osteoblasts in vitro differentiate from a proliferating to a mineralizing phenotype upon transfer to a medium rich in beta-glycerophosphate and ascorbic acid. The nutritional requirements of the cells at different stages of this differentiation process are not known. In other cell types, nutritional supplementation du...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-011-9537-6
更新日期：2011-12-01 00:00:00
abstract：:Bone turnover markers (BTMs) are widely used for the management of osteoporosis, and the premenopausal reference range is the target value for the treatment of postmenopausal osteoporosis with antiresorbing agents. Three serum BTMs (serum C-telopeptide of type I collagen [CTX], osteocalcin [OC], and N-terminal propept...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9126-5
更新日期：2008-05-01 00:00:00
abstract：:Bone resorption and formation in terms of milligram of calcium deposited into and released from whole humerus of 15-23 weeks old male rats were assessed, via linear least-squares regression analysis of change in calcium content and 3H-tetracycline kinetics of the bone. The rate of resorption was 0.376 mg calcium per d...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555009
更新日期：1990-04-01 00:00:00
abstract：:Ectopic calcification of diseased tissues or around prosthetic implants can lead to serious disability. Therefore, calcification of implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen was compared with mineralization induced by demineralized bone matrix (DBM). Whereas implants of DBM...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556366
更新日期：1988-05-01 00:00:00
abstract：:Osteoporosis is characterized by low bone mass and a deterioration of bone architecture and likely is influenced by genetic factors. The ovariectomized (OVX) mouse is well suited for osteoporosis research, as shown to date by cross-sectional studies. Here, we investigate longitudinal changes by in vivo micro-computed ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9150-5
更新日期：2008-07-01 00:00:00
abstract：:Enamel of intact human teeth laser irradiated in vitro under certain conditions is known to have less subsurface demineralization than unirradiated enamel on exposure to acid; consequently, the potential use of laser irradiance to reduce caries is apparent. The laser-induced physical and/or chemical changes that cause...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/BF02556711
更新日期：1986-04-01 00:00:00
abstract：:Sclerostin and parathyroid hormones are strong negative and positive regulators of bone formation, respectively. The anabolic response induced by intermittent (iPTH) treatment is sclerostin status-dependent. However, the interaction between sclerostin and iPTH at the matrix level is unknown. The goal of the current st...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-015-0074-6
更新日期：2016-02-01 00:00:00
abstract：:This article reviews the effects of restrictive eating disorders on bone health. The relationship between eating disorders and amenorrhea is discussed in detail. The pathologic impact of malnutrition on bone is explored by examining the results of studies using various available imaging techniques. The multiple hormon...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-016-0164-0
更新日期：2017-05-01 00:00:00
abstract：:Osteoarthritis (OA), the most prevalent chronic joint disease, increases in prevalence with age, and affects majority of individuals over the age of 65 and is a leading musculoskeletal cause of impaired mobility in the elderly. Because the precise molecular mechanisms which are involved in the degradation of cartilage...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-014-9917-9
更新日期：2014-12-01 00:00:00
abstract：:Osteoporosis is a bone disease leading to an increased fracture risk. It is considered a complex multifactorial genetic disorder with interaction of environmental and genetic factors. As a candidate gene for osteoporosis, we studied vitamin D binding protein (DBP, or group-specific component, Gc), which binds to and t...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-009-9251-9
更新日期：2009-08-01 00:00:00
abstract：:Aging and sex hormones related changes lead to inflammatory and oxidant conditions, which are involved in the pathogenesis of osteoporosis. Recent studies have suggested that polyphenols may exert a protective effect in such conditions. We assessed the effect of phloridzin (Phlo), a flavonoid exclusively found in appl...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0060-5
更新日期：2005-11-01 00:00:00
abstract：:The present report compares the effects of different membrane phospholipid (PL)-cholesterol compositions on the kinetics of liposome-mediated formation of calcium phosphates from metastable solutions (2.25 mM CaCl2; 1.5 mM KH2PO4) at 22 degrees C, pH 7.4 and 240 mOsm. In most experiments, the liposomes were composed o...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296290
更新日期：1992-03-01 00:00:00
abstract：:The effect of long-chain n-3 polyunsaturated fatty acid (PUFA) on bone measurements was evaluated in gonad-intact middle-aged male rats. Seven rats were killed on day 0 of dietary intervention to determine bone parameters at baseline. Experimental rats (7/group) were fed one of the following lipid treatments (g/kg die...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9010-8
更新日期：2007-04-01 00:00:00
abstract：:Patients put at bedrest for medical reasons lose 1-2% of spinal bone mineral per week. Losses of this magnitude during even short-term space flights of a few months would pose a serious limitation and require countermeasures. The spinal bone mineral (L2-L4) was determined in 6 healthy males (precision = 2%) before and...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555226
更新日期：1987-11-01 00:00:00
abstract：:Vitamin D supplementation is recommended for women with osteoporosis. In the FOCUS-D trial comparing the combination tablet alendronate plus vitamin D3 5,600 IU (ALN/D) with standard care (SC) prescribed by patients' personal physicians, ALN/D was more effective in improving serum 25(OH)D and bone turnover markers by ...
journal_title：Calcified tissue international
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00223-013-9763-1
更新日期：2014-02-01 00:00:00
abstract：:There is considerable interest in determining whether hypergravity can be used as a countermeasure for microgravity-induced bone loss. This study was conducted on 20 immature male rats in order to investigate possible elastic adaptations of cortical bone in rapidly growing rats exposed to chronic hypergravity. Ten rat...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900111
更新日期：1996-09-01 00:00:00
abstract：:Limited information is available on the effect of bisphosphonates in men receiving corticosteroid therapy. We studied 184 men among the patients enrolled in two, double-blind, placebo-controlled, 1-year studies with similar protocols. The studies evaluated the effects of risedronate in patients beginning corticosteroi...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00223-001-1060-8
更新日期：2001-10-01 00:00:00
abstract：:The development of prophylaxes and treatments of bone diseases that can effectively increase the strength of bone as a structure necessitates a better understanding of the time course by which chemical properties define the stiffness of the material during primary and secondary mineralization. It was hypothesized that...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-005-0148-y
更新日期：2005-12-01 00:00:00
abstract：:The purpose of this study was to examine the influence of exercise on the serum concentrations of intact parathyroid hormone (PTH). Serum PTH and plasma lactate were measured in 15 well-trained men, 9 long-distance runners and 6 fire-fighters, during two running exercises. Test one consisted of 40-minute treadmill run...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00295947
更新日期：1994-04-01 00:00:00
abstract：:Newly developed fuzzy logic-derived structural parameters were used to characterize trabecular bone architecture in high-resolution magnetic resonance imaging (HR-MRI) of human cadaver calcaneus specimens. These parameters were compared to standard histomorphological structural measures and analyzed concerning perform...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-007-9058-5
更新日期：2007-10-01 00:00:00
abstract：:The effect of teriparatide treatment on microdamage accumulation has yet to be examined in animal studies. The purpose of this study was to investigate the effect of once-weekly teriparatide treatment on bone microdamage accumulation and the relationship between microdamage parameters and bone mass, architecture, turn...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-018-0500-7
更新日期：2019-04-01 00:00:00
abstract：:KH1060 is a new 20-epi-vitamin D3 analog, which has exerted a considerable immunosuppressive potency in vitro. We have tested in vivo the effect of KH1060 on the suppression of renal allograft rejection in the rat. Allogenic kidney transplantation from DA donor rats to Lewis recipient rats treated intraperitoneally wi...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296066
更新日期：1994-02-01 00:00:00
abstract：:GNAS is one of the most complex gene loci in the human genome and encodes multiple gene products including Gsα, XLαs, NESP55, A/B, and AS transcripts. XLαs, the extra-large G protein ɑ-subunit, is paternally expressed. XLɑs and Gsɑ share the common 2-13 exons with different promoters and first exons. Therefore, XLɑs c...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-020-00714-2
更新日期：2020-09-01 00:00:00
abstract：:The relationship between bisphosphonate-induced bone mineral density (BMD) gains and antifracture efficacy remains to be fully elucidated. Data from two antifracture studies were analyzed. Postmenopausal osteoporotic women received oral (2.5 mg daily, 20 mg intermittent) or intravenous (0.5 mg, 1 mg quarterly) ibandro...
journal_title：Calcified tissue international
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00223-010-9403-y
更新日期：2010-10-01 00:00:00
abstract：:The association of dietary patterns and bone health is not yet well known, and findings from the rare previous studies conducted on this issue are contradictory. We assessed the dietary patterns in relation to bone mineral density (BMD) in a sample of menopausal Iranian women. In this cross-sectional study, 160 menopa...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-012-9608-3
更新日期：2012-07-01 00:00:00
abstract：:Experimental analysis with paired femurs provides the opportunity to study within-person differences in fracture type and associated structural side differences. We hypothesized that different fracture types in the hips of a subject are associated with structural asymmetry. Bone mineral density (BMD) and structural me...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-010-9388-6
更新日期：2010-09-01 00:00:00
abstract：:We compared the cytotoxic effects of alendronate (ALN) and incadronate (YM175) on isolated rabbit osteoclasts in vitro and on rats in vivo. In the in vitro experiment, each bisphosphonate was added to the culture of isolated osteoclasts at the final concentration of 3 x 10(-5), 3 x 10(-4), or 3 x 10(-3) M, and the amo...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900505
更新日期：1998-08-01 00:00:00
abstract：:This study aimed to evaluate the association between objectively measured habitual physical activity and calcaneal and forearm bone mineral density (BMD, g/cm(2)), one mechanically more loaded and one less loaded skeletal region, in children aged 6-8 years. BMD was measured in 297 boys and 265 girls by peripheral dual...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-006-0137-9
更新日期：2007-01-01 00:00:00
abstract：:Results of previous studies suggest that children and adolescents with osteogenesis imperfecta (OI) type IV have muscle force deficits. However, muscle function remains to be objectively quantified in this population. This study aimed to assess upper and lower extremity muscle function in patients with OI type IV. It ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-017-0287-y
更新日期：2017-10-01 00:00:00
