Abstract：
:Osteoblasts in vitro differentiate from a proliferating to a mineralizing phenotype upon transfer to a medium rich in beta-glycerophosphate and ascorbic acid. The nutritional requirements of the cells at different stages of this differentiation process are not known. In other cell types, nutritional supplementation during surgery can improve the outcome in terms of speed of patient recovery and prognosis. There is therefore the potential for supplementation at the site of fracture repair or bone grafting with critical osteoblast nutritional factors to potentially accelerate healing. In this study we investigate which common cell nutrients are required for the proliferating and mineralizing stages of osteoblast differentiation. Medium containing 5.5 mM glucose was sufficient to achieve maximal proliferation of primary calvarial osteoblasts and human osteoblast cell lines, with some added benefit of additional glutamine supplementation. However, when cells were stimulated to mineralize, glucose was insufficient to support their energetic requirements. Only when cells were supplemented with glucose together with glutamine were high levels of osteocalcin expression observed together with mineralized nodules in culture, suggesting that this would be a useful combination to assess in cultures of primary human osteoblasts to determine whether it may have beneficial effects during fracture surgery, bone grafting, and fixation of uncemented arthroplasty implants.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Brown PM,Hutchison JD,Crockett JCdoi
10.1007/s00223-011-9537-6subject
Has Abstractpub_date
2011-12-01 00:00:00pages
472-82issue
6eissn
0171-967Xissn
1432-0827journal_volume
89pub_type
杂志文章abstract：:It is generally accepted that bone formation is depressed during corticosteroid treatment, but the effects of glucocorticoids on bone resorption are less well characterized. We have investigated the effects of short-term treatment with high-dose oral glucocorticoids on biochemical markers of bone turnover in 20 consec...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900482
更新日期：1998-07-01 00:00:00
abstract：:We have recently reported that risedronate preserves normal bone formation and decreases bone remodeling in women with postmenopausal osteoporosis after 3 years of treatment. We report now the results of a 2-year extension study. The primary objective of this study was to determine the effect of 5 years of risedronate...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/s00223-004-0039-7
更新日期：2004-12-01 00:00:00
abstract：:There is increasing evidence that 1,25 dihydroxyvitamin D3 acts directly to differentiate and activate cells of mononuclear phagocyte lineage (MNP). However, it seems possible that in bone marrow this hormone may modulate the differentiation of MNP via the factor elaborated by the stromal cells or the macrophages. We ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02556592
更新日期：1986-01-01 00:00:00
abstract：:Proton pump inhibitors are taken by millions of patients for prevention and treatment of gastroesophageal diseases. Case-control studies have suggested that use of omeprazole is associated with an increased risk of hip fractures. The aim of this prospective study was to assess the risk of vertebral fractures in postme...
journal_title：Calcified tissue international
pub_type： 杂志文章,多中心研究
doi：10.1007/s00223-008-9188-4
更新日期：2009-01-01 00:00:00
abstract：:Cortical bone can be modeled as a complex hierarchical composite interrelating both structure and material properties on four levels of structural organization: molecular, ultrastructural, microscopic, and macroscopic. In young animals, the microstructural systems are long parallel lamellar units, plexiform bone, whic...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02406131
更新日期：1984-01-01 00:00:00
abstract：:Vitamin K promotes the formation of gamma-carboxylated glutamate (GLA) in several protein species. GLA residues have a high affinity for the Ca ion. In the present study, we tested the hypothesis that experimental vitamin K deficiency in rats could induce changes in Ca metabolism. Vitamin K depletion, which was associ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02554833
更新日期：1985-03-01 00:00:00
abstract：:In 11 postmenopausal women with histologically proven osteoporosis and normal lactose tolerance, a direct correlation between calcium absorption and intestinal lactase activity was observed. The results suggest that varying degrees of relative deficiencies in intestinal lactase might contribute to the graded decrease ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02557688
更新日期：1985-01-01 00:00:00
abstract：:The purpose of this research was to address the following question: How is the solubility of fluoride-pretreated carbonated apatite (CAP) in aqueous acidic media related to the equilibrium solution fluoride and/or the CAP adsorbed fluoride levels? A CAP sample prepared by a precipitation method at 70 degrees C contain...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-1069-z
更新日期：2003-03-01 00:00:00
abstract：:Proteoglycans are recognized to inhibit calcium pyrophosphate dihydrate (CPPD) crystal formation but the mechanisms are not known. To study the role of carboxylate (-CO2-) ligands, the possible inhibitor effects of sodium acetate, sodium D-glucuronate, disodium malate, and trisodium citrate were studied using solution...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02555838
更新日期：1988-01-01 00:00:00
abstract：:We describe here the activity of a novel selective estrogen receptor modulator, SP500263. When given to adult ovariectomized (OVX) rats for 28 days at doses of 0.3, 1, or 3 mg/kg/day, we found that SP500263 partially protected against OVX-induced loss of bone mineral content in the distal ends of femurs and in the who...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-002-1029-2
更新日期：2003-06-01 00:00:00
abstract：:Osteoarthritis (OA), the most prevalent chronic joint disease, increases in prevalence with age, and affects majority of individuals over the age of 65 and is a leading musculoskeletal cause of impaired mobility in the elderly. Because the precise molecular mechanisms which are involved in the degradation of cartilage...
journal_title：Calcified tissue international
pub_type： 杂志文章,评审
doi：10.1007/s00223-014-9917-9
更新日期：2014-12-01 00:00:00
abstract：:To assess the usefulness of the measurement of the os calcis by ultrasound, a method that probably reflects bone quality as well as density, we have studied 54 women with hip fracture of the proximal femur and a control group. Ultrasound evaluation of the os calcis [broadband ultrasound attenuation (BUA), speed of the...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00310252
更新日期：1995-09-01 00:00:00
abstract：:Interleukin (IL)-6 is a bone-resorbing cytokine that acts primarily on osteoclast progenitors to stimulate both proliferation and differentiation. Glucocorticoids (GC) down-regulate IL-6 synthesis in different cell types, including osteoblasts. Given the fact that bone remodeling is a tightly controlled process, it is...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-001-2024-8
更新日期：2001-11-01 00:00:00
abstract：:In humans, gastric surgery results in in osteopenia via mechanisms that are insufficiently understood; surgery-induced changes in the hormonal axes involving the stomach, thyroid, and the parathyroids may play a role. To study this in more detail, we evaluated calcium (Ca), magnesium (Mg), and phosphorus (P) metabolis...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002239900553
更新日期：1998-11-01 00:00:00
abstract：:The effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (2.3 x 10(-12) - 1.4 x 10(-6) [M]) on alkaline phosphatase, collagen, and cell proliferation were examined in primary cultured hypertrophic chondrocytes prepared from the distal epiphyseal growth plate of the tibias of 12-day chick embryos. 1,25(OH)2D3 showed time-...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF00296776
更新日期：1992-05-01 00:00:00
abstract：:This study was conducted to determine the effectiveness of three forms of vitamin E supplements following nicotine treatment on bone histomorphometric parameters in an adult male rat model. Rats were divided into seven groups: baseline (B, killed without treatment), control (C, normal saline for 4 months), nicotine (N...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-008-9190-x
更新日期：2009-01-01 00:00:00
abstract：:The purpose of this study was to estimate clinical validity of a new available immunoradiometric assay for circulating intact human BGP (N-tact Osteo SP) by measuring this protein in a large number of normal subjects and patients with the most common metabolic bone diseases. One hundred normal subjects were studied in...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005815
更新日期：1999-05-01 00:00:00
abstract：:Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane wa...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-020-00793-1
更新日期：2021-01-09 00:00:00
abstract：:The objective of this study was to compare the bone mineral density (BMD) of men with Down syndrome (DS) to otherwise mentally retarded (MR) men and to investigate whether leg muscle strength of these patients is related to BMD. Two groups with MR (with and without DS) participated in the study, having met the followi...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s002230010035
更新日期：2000-03-01 00:00:00
abstract：:We compared indices of three-dimensional microstructure of iliac trabecular bone between 26 patients with vertebral compression fractures due to postmenopausal osteoporosis and 24 control subjects without vertebral fracture, who were matched for age, sex, race, menopausal status, and several densitometric and histolog...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02554913
更新日期：1985-12-01 00:00:00
abstract：:We had shown that aromatic amino acid (phenylalanine, tyrosine, and tryptophan) supplementation prevented bone loss in an aging C57BL/6 mice model. In vivo results from the markers of bone breakdown suggested an inhibition of osteoclastic activity or differentiation. To assess osteoclastic differentiation, we examined...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-014-9878-z
更新日期：2014-08-01 00:00:00
abstract：:Macroporous biphasic calcium phosphate (MBCP) blocks were implanted into rabbit trabecular bone and muscle, recovered 18 weeks later, and then observed and analyzed by transmission electron microscopy (TEM), electron diffraction, and electron microprobes. The results showed that (1) apatitic microcrystals appeared by ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005825
更新日期：1999-05-01 00:00:00
abstract：:Formalin fixation of hard tissues alters the levels of some, but not all, of the parameters routinely measured by bone researchers. Although mineral parameters (ash weight, particle size (beta 1/2 002) and Ca/PO4 ratio) and lipid parameters (total lipid content and composition, extractability, and Ca-acidic phospholip...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02411262
更新日期：1982-07-01 00:00:00
abstract：:Orthopaedic wear debris induces release of bone-resorbing factors from macrophages and fibroblasts. However, the extent to which elemental metallic particles induce bone cells to express factors contributing to implant loosening remains unclear. This study showed that exposure of MG-63 osteoblast-like cells to titaniu...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223001125
更新日期：2000-08-01 00:00:00
abstract：:We examined differences in patients' survival after hip fracture (HF) and risk for subsequent HF among patients treated with oral and intravenous bisphosphonates (oBPs, iBPs), denosumab (DMAB), and patients without therapy. We used data from all patients in Austria aged ≥ 50 who sustained a HF between 2012 and 2017 an...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-019-00611-3
更新日期：2019-12-01 00:00:00
abstract：:Lumbar spine bone mass and density were measured with Dual photon absorptiometry (DPA) in 60 patients with crush fractures and 60 age-matched normal women. Short-term reproducibility of bone mineral density (BMD) was 1.3% in normal women and 2.5% in osteoporotic women; long-term reproducibility in normal women was 2.2...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02553274
更新日期：1988-12-01 00:00:00
abstract：:The purpose of this study was to determine whether human parathyroid hormone (h-PTH) enhances trabecular bone mass and connectivities that were reduced by streptozotocin (STZ)-induced diabetes in rats. Seven-month-old female Wistar rats were injected with STZ or its vehicle intraperitoneally. All vehicle-injected norm...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/pl00005838
更新日期：2000-03-01 00:00:00
abstract：:Several studies have shown an association between circulating leptin concentration and bone mineral density. but most studies are cross-sectional in design and report findings in women only. We per-formed a population-based longitudinal study relating baseline plasma leptin concentration to bone mass at the lumbar spi...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/s00223-002-0017-x
更新日期：2004-05-01 00:00:00
abstract：:Seventy-two postmenopausal osteoporotic women having more than one nontraumatic vertebral crush fracture were studied. Thirty-six of them, aged 68.8 +/- 1.2 years (18 +/- 4 YSM-years since menopause), were treated with 100 IU/day of salmon calcitonin i.m. plus 500 mg of elemental calcium for 10 days each month. The re...
journal_title：Calcified tissue international
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00298606
更新日期：1995-03-01 00:00:00
abstract：:The effects of a series of novel phosphonates on the kinetics of mineral development in an ionophore-primed 7:2:1 phosphatidylcholine (PC): dicetylphosphate (DCP): cholesterol (Chol) liposomal model system are reported. When present at 2.5 micromol/liter or 25 micromol/liter concentrations in the solution surrounding ...
journal_title：Calcified tissue international
pub_type： 杂志文章
doi：10.1007/BF02509384
更新日期：1996-05-01 00:00:00
