Abstract:
:The development of prophylaxes and treatments of bone diseases that can effectively increase the strength of bone as a structure necessitates a better understanding of the time course by which chemical properties define the stiffness of the material during primary and secondary mineralization. It was hypothesized that these processes would be relatively slow in the actively growing skeleton. Seven-week-old Sprague-Dawley female rats (n = 8) were injected with multiple fluorochrome labels over a time span of 3 weeks and killed. Chemical and mechanical properties of the tibial mid-diaphysis were spatially characterized between the endocortical and periosteal surface by in situ infrared microspectroscopy and nanoindentation. The phosphate-to-protein ratio of bone 2-6 days old was 20% smaller at the periosteal surface and 22% smaller at the endocortical surface (P < 0.05 each) compared to older intracortical regions. The ratios of carbonate to protein, crystallinity, type A/type B carbonate, collagen cross-linking, and bone elastic modulus did not differ significantly between bone 2-6, 10-14, and 8-22 days old and intracortical regions. Intracortical properties of 10-week-old rats, except for the carbonate-to-protein ratio which was 23% smaller (P < 0.01), were not significantly different from intracortical matrix properties of young adult rats (5 months, n = 4). Spatially, the phosphate-to-protein ratio (R(2) = 0.33) and the phosphate-to-carbonate ratio (R(2) = 0.55) were significantly correlated with bone material stiffness, while the combination of all chemical parameters raised the R(2) value to 0.83. These data indicate that lamellar bone has the ability to quickly establish its mechanical and chemical tissue properties during primary and secondary mineralization even when the skeleton experiences rapid growth.
journal_name
Calcif Tissue Intjournal_title
Calcified tissue internationalauthors
Busa B,Miller LM,Rubin CT,Qin YX,Judex Sdoi
10.1007/s00223-005-0148-ysubject
Has Abstractpub_date
2005-12-01 00:00:00pages
386-94issue
6eissn
0171-967Xissn
1432-0827journal_volume
77pub_type
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
pub_type: 杂志文章
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
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journal_title:Calcified tissue international
pub_type: 临床试验,杂志文章
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journal_title:Calcified tissue international
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