A phorbol ester induces secretion of alkaline phosphatase activity in human osteosarcoma cells.

Abstract:

:The phorbol ester 12-O-tetradecanoyl-13-acetate (TPA) blocked the growth of, and induced the appearance of processes in the human osteosarcoma cell line U-2 OS. The phorbol ester decreased the intracellular level of alkaline phosphatase (APase) activity (as measured per mg cell protein) and caused a marked increase in the APase activity secreted from the cells into the culture medium. The secretion of APase appeared after a lag period of 4-6 hours of TPA treatment, and it could also be visualized with histological staining. Differential ultracentrifugation of the culture media showed that the APase was released to the media in the form of vesicles. The vesicles were studied by electron microscopy and appeared similar to matrix vesicles isolated from cartilage and chondrocytes. It is thus concluded that TPA is able to induce the primary steps of mineralization in these cells.

journal_name

Calcif Tissue Int

authors

Ringbom-Anderson T,Akerman KE

doi

10.1007/BF00582169

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

533-40

issue

6

eissn

0171-967X

issn

1432-0827

journal_volume

50

pub_type

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