Demonstration of calmodulin-sensitive calcium translocation by isolated osteoclast plasma membrane vesicles.

Abstract:

:Plasma membrane vesicles were prepared from chicken osteoclasts, and active calcium transport was demonstrated in a spectrofluorimetric assay using the fluorescent calcium concentration indicator, fura-2. Transport activity was inhibited by quercetin (10 microM), sodium vanadate (10 microM), and the anticalmodulin agents, compound 48/80 (20 and 200 micrograms/ml) and calmidazolium (10 and 20 microM). The transport rate (Vmax, 1.3 nmol/mg protein/min) was not altered in the presence of the protonophore, nigericin (1 microM), indicating that proton transport was not driving calcium transport. Release of accumulated calcium in the vesicles occurred with the addition of bromo-A23187 (5 microM) or ionomycin (5 microM). Increasing calcium transport occurred with increasing calcium concentration. Finally, the calmodulin content of the vesicles was demonstrated to be 54-134 U/mg protein. These results demonstrate that a calmodulin-sensitive, ATP-dependent calcium transporter is present in the osteoclast plasma membrane.

journal_name

Calcif Tissue Int

authors

Bekker PJ,Gay CV

doi

10.1007/BF00334493

subject

Has Abstract

pub_date

1992-10-01 00:00:00

pages

312-6

issue

4

eissn

0171-967X

issn

1432-0827

journal_volume

51

pub_type

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