Abstract：
:Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the alpha-subunit of Gq (Ad-EF1-dnG alpha q) and the other expressing the wild-type alpha-subunit of Gq (Ad-EF1-wtG alpha q). We used an adenovirus expressing green fluorescent protein (Ad-EF1-GFP20) to show that infection of cultured cells with an adenovirus results in at least 95% expression of the transgene in both HSG and HT29 cells. We also used an adenovirus that expresses no transgene (Ad-MX17) to demonstrate that adenoviral infection itself does not affect the resting concentration of cytosolic Ca2+ ([Ca2+]i) or the carbachol responses in these cells. We further show that Ad-EF1-dnG alpha q inhibits the increase in [Ca2+]i produced by muscarinic receptor activation in both the cell lines we studied. This inhibitory effect is not shared by Ad-EF1-wtG alpha q, which indicates that in both HSG and HT29 cells, the increase in [Ca2+]i produced by muscarinic receptor activation is largely mediated by activation of Gq. Neither virus affected the resting level of [Ca2+]i in these cells. Our findings confirm the feasibility of using replication deficient adenoviruses expressing dominant negative mutants to investigate the role of G proteins in signal transduction systems.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Poronnik P,O'Mullane LM,Harding EA,Greger R,Cook DIdoi
10.1016/s0143-4160(98)90077-xsubject
Has Abstractpub_date
1998-08-01 00:00:00pages
97-103issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(98)90077-Xjournal_volume
24pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90008-i
更新日期：1990-04-01 00:00:00
abstract：:We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90029-9
更新日期：1994-11-01 00:00:00
abstract：:Dendritic Ca2+ plays an important role not only in synaptic integration and synaptic plasticity, but also in dendritic excitability in midbrain dopamine neurons. However, the functional organization of dendritic Ca2+ signals in the dopamine neurons remains largely unknown. We therefore investigated dendritic Ca2+ sign...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.06.007
更新日期：2011-10-01 00:00:00
abstract：:Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90033-6
更新日期：1997-02-01 00:00:00
abstract：:Specific Ca(2+) homeostatic system appeared very early in the history of the cell, as a survival system preventing Ca(2+)-mediated cell damage. This homeostatic system produced a steep ( approximately 20,000 times) concentration gradient between extracellular and intracellular compartments, which has both survival imp...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2007.05.001
更新日期：2007-10-01 00:00:00
abstract：:Store-operated Ca2+ entry (SOCE), an important Ca2+ signaling pathway in non-excitable cells, regulates a variety of cellular functions. To study its physiological role, pharmacological tools, like 2-aminoethyl diphenylborinate (2-APB), are used to impact SOCE. 2-APB is one of the best characterized SOCE inhibitors. H...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2017.01.015
更新日期：2017-03-01 00:00:00
abstract：:The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.12.001
更新日期：2009-04-01 00:00:00
abstract：:The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.01.014
更新日期：2004-06-01 00:00:00
abstract：:Stromal interaction molecule (STIM)-1 and -2 are multi-domain, single-pass transmembrane proteins involved in sensing changes in compartmentalized calcium (Ca2+) levels and transducing this cellular signal to Orai1 channel proteins. Our understanding of the molecular mechanisms underlying STIM signaling has been drama...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:Intracellular Ca(2+) signals are essential for stem cell differentiation due to their ability to control signaling pathways involved in this process. Arginine vasopression (AVP) is a neurohypophyseal hormone that increases intracellular Ca(2+) concentration during adipogenesis via V1a receptors, Gq-proteins and the PL...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2015.12.006
更新日期：2016-03-01 00:00:00
abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel which plays a major role in Ca2+ signalling. Three isoforms of IP3R have been identified (IP3R-1, IP3R-2 and IP3R-3) and most cell types express different proportions of each isoform. The differences between the p...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.01.002
更新日期：2007-09-01 00:00:00
abstract：:STIM and Orai isoforms orchestrate store operated Ca2+ entry (SOCE) and thus cytosolic Ca2+ fluctuations following stimulation by hormones, growth factors and further mediators. Orai1 is a target of Nedd4-2, an ubiquitin ligase preparing several plasma membrane proteins for degradation. Phosphorylation of Nedd4-2 by t...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2012.05.005
更新日期：2012-11-01 00:00:00
abstract：:A variety of stimuli utilize an increase of cytosolic free Ca(2+) concentration as a second messenger to transmit signals, through Ca(2+) release from the endoplasmic reticulum or opening of plasma membrane Ca(2+) channels. Mitochondria contribute to the tight spatiotemporal control of this process by accumulating Ca(...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.04.007
更新日期：2011-09-01 00:00:00
abstract：:The highly differentiated structure of the neuron poses special problems for the intracellular movement of molecules throughout the cell. Molecular transport distances from the synthesizing neuron cell body along the axon (which has no substantial synthetic capabilities) to the axon terminal are very great. The transp...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(88)90010-3
更新日期：1988-12-01 00:00:00
abstract：:The effects of the unsaturated fatty acids, arachidonic and oleic acid, on the influx of Ca2+ activated by depletion of intracellular stores with thapsigargin were investigated in various cell types. By using a Ca2+ free/Ca2+ reintroduction protocol, we observed that arachidonic acid (2 to 5 microM) inhibited thapsiga...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90031-2
更新日期：1997-05-01 00:00:00
abstract：:We have previously demonstrated a role for the reorganization of the actin cytoskeleton in store-operated calcium entry (SOCE) in human platelets and interpreted this as evidence for a de novo conformational coupling step in SOCE activation involving the type II IP(3) receptor and the platelet hTRPC1-containing store-...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.02.004
更新日期：2007-12-01 00:00:00
abstract：:The mammalian transient receptor potential (TRP) proteins consist of a superfamily of Ca2+-permeant non-selective cation channels with structural similarities to Drosophila TRP. The TRP superfamily can be divided into three major families, among them the "canonical TRP" family (TRPC). The seven protein products of the...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00056-3
更新日期：2003-05-01 00:00:00
abstract：:In the cochlea, cell damage triggers intercellular Ca2+ waves that propagate through the glial-like supporting cells that surround receptor hair cells. These Ca2+ waves are thought to convey information about sensory hair cell-damage to the surrounding supporting cells within the cochlear epithelium. Mitochondria are ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.06.005
更新日期：2009-08-01 00:00:00
abstract：:The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0087
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Previous studies suggest that CaMKII activity is required for frequency-dependent acceleration of relaxation (FDAR) in ventricular myocytes. We propose that the underlying mechanism involves CaMKII-dependent regulation of myofilament Ca(2+) sensitivity. METHODS AND RESULTS:Cardiac function was measured in m...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.08.001
更新日期：2015-11-01 00:00:00
abstract：:The surface dynamics of neurotransmitter receptors and transporters, as well as ion channels, has been well-documented in neurons, revealing complex molecular behaviour and key physiological functions. However, our understanding of the membrane trafficking and dynamics of the signalling molecules located at the plasma...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.08.009
更新日期：2017-11-01 00:00:00
abstract：:Antigen-mediated mast cell (MC) degranulation is the critical early event in the induction of allergic reactions. Transient receptor potential channels (TRPC), particularly TRPC1, are thought to contribute to such MC activation. To explore the contribution of TRPC1 in MC-driven allergic reactions, we examined antigen-...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.02.001
更新日期：2013-05-01 00:00:00
abstract：:Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.04.008
更新日期：2016-08-01 00:00:00
abstract：:We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2004.05.002
更新日期：2004-12-01 00:00:00
abstract：:The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washe...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(87)90002-9
更新日期：1987-08-01 00:00:00
abstract：:The Ca(2+) and cAMP/PKA pathways are the primary signaling systems in secretory epithelia that control virtually all secretory gland functions. Interaction and crosstalk in Ca(2+) and cAMP signaling occur at multiple levels to control and tune the activity of each other. Physiologically, Ca(2+) and cAMP signaling oper...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2014.01.006
更新日期：2014-06-01 00:00:00
abstract：:NAADP has been shown to act as a second messenger in a wide range of systems from plants to mammalian cells. Although it had always been considered as a canonical second messenger, recent work has shown that it is also active when applied extracellularly. It has also been suggested that NAADP might have a direct actio...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.10.002
更新日期：2007-06-01 00:00:00
abstract：:The activation of T lymphocytes, like many cells, requires a rapid rise in intracellular Ca2+ secondary to both an influx and a release from intracellular stores. The latter is activated by inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. It is controversial if inositol phosphates can also stimulate a plasma membrane Ca2+...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90082-h
更新日期：1993-02-01 00:00:00
abstract：:Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2005.01.018
更新日期：2005-05-01 00:00:00
abstract：:The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.03.004
更新日期：2014-07-01 00:00:00
