Abstract：
:The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washes to remove extracellular dye, the cells were resuspended for fluorescence measurements. Extracellular Ca2+ was 2.0 mM. Resting Cai2+ in the rat (121 +/- 11 nM) was found to be significantly higher than in the cat (57 +/- 4 nM). These results are discussed in terms of known differences in excitation-contraction coupling between these two species.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
duBell WH,Houser SRdoi
10.1016/0143-4160(87)90002-9subject
Has Abstractpub_date
1987-08-01 00:00:00pages
259-68issue
4eissn
0143-4160issn
1532-1991pii
0143-4160(87)90002-9journal_volume
8pub_type
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