Abstract：
:Agonist-induced calcium (Ca) mobilization is accompanied by Ca efflux, presumably reflecting the rise in Ca concentration at the cytosolic surface of the cell membrane. We studied the relationship between Ca efflux and intracellular Ca mobilization in Xenopus oocytes. Elevation of cytosolic Ca by a direct injection of 1 nmol 45CaCl2 resulted in a typical Ca-activated chloride current, but not in 45Ca efflux. This demonstrated that a Ca rise at the cytoplasmic surface of the membrane is not sufficient to produce an increased efflux. Co-injection of inositol 1,4,5-trisphosphate (InsP3), to prevent rapid Ca sequestration, also failed to cause Ca efflux. Smaller amounts of labelled Ca (0.05 nmol) equilibrated with Ca stores in a time-dependent pattern with an optimum at 2 h after injection. In contrast, Ca taken up from the medium was immediately available for agonist- or InsP3-induced efflux. Emptying the agonist-sensitive stores with thapsigargin (TG) did not affect chloride currents induced by Ca injection, indicating that these currents were due to direct elevation of Ca at the plasma membrane, rather than Ca-induced Ca release from InsP3-sensitive stores. Agonist-induced depletion of Ca stores enhanced uptake from the extracellular medium and the subsequent release of the label by an agonist. Similar protocol when the label was injected into the oocytes, failed to affect agonist induced efflux. We suggest that, under physiological conditions, agonist-dependent Ca extrusion or uptake in oocytes is executed exclusively via a functionally restricted compartment, which is closely associated with both agonist-sensitive Ca stores and the plasma membrane.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Shapira H,Lupu-Meiri M,Lipinsky D,Oron Ydoi
10.1016/s0143-4160(96)90021-4subject
Has Abstractpub_date
1996-03-01 00:00:00pages
201-10issue
3eissn
0143-4160issn
1532-1991pii
S0143-4160(96)90021-4journal_volume
19pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.11.001
更新日期：2012-03-01 00:00:00
abstract：:One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid met...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.12.008
更新日期：2017-06-01 00:00:00
abstract：:The effects of the thyroid status on the Ca++-transporting capabilities of rat slow skeletal muscle (m.soleus) were studied. The oxalate supported Ca++-uptake activity and Ca++-loading capacity of muscle homogenates from hyperthyroid rats showed an approximate 4.2 and 2.5 fold increase, respectively, as compared to va...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(86)90018-7
更新日期：1986-06-01 00:00:00
abstract：:The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by Ca...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2017.05.013
更新日期：2017-09-01 00:00:00
abstract：:Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2021.102358
更新日期：2021-01-23 00:00:00
abstract：:Great insight has been gained into the structure and function of the inositol 1,4,5 trisphosphate receptor (InsP(3)R) by studies employing mutagenesis of the cDNA encoding the receptor. Notably, early studies using this approach defined the key constituents required for InsP(3) binding in the N-terminus and the membra...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2010.04.005
更新日期：2010-06-01 00:00:00
abstract：:Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be pr...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.02.001
更新日期：2011-03-01 00:00:00
abstract：:It is well established that intracellular calcium ([Ca2+]i) controls the inotropic state of the myocardium, and evidence mounts that a "Ca2+ clock" controls the chronotropic state of the heart. Recent findings describe a calcium-activated nonselective cation channel (NSCCa) in various cardiac preparations sharing hall...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.06.003
更新日期：2013-09-01 00:00:00
abstract：:The purpose of this study was to invent an extracellular inhibitor selective for the plasma membrane Ca(2+) pump(s) (PMCA) isoform 1. PMCA extrude Ca(2+) from cells during signalling and homeostasis. PMCA isoforms are encoded by 4 genes (PMCA1-4). Pig coronary artery endothelium and smooth muscle express the genes PMC...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.10.008
更新日期：2010-12-01 00:00:00
abstract：:Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0044
更新日期：1999-06-01 00:00:00
abstract：:We have previously demonstrated a role for the reorganization of the actin cytoskeleton in store-operated calcium entry (SOCE) in human platelets and interpreted this as evidence for a de novo conformational coupling step in SOCE activation involving the type II IP(3) receptor and the platelet hTRPC1-containing store-...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.02.004
更新日期：2007-12-01 00:00:00
abstract：:The endoplasmic reticulum (ER) acts as a dynamic calcium store and is involved in the generation of specific patterns of calcium signals in neurons. Calcium is mobilized from the ER store by multiple signaling cascades, and neuronal activity is known to regulate ER calcium levels. We asked how neurons regulate ER calc...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.04.002
更新日期：2015-08-01 00:00:00
abstract：:Calcium is a major intracellular signaling messenger in innate immune cells. Similar to other immune cell subsets, the majority of calcium entry into innate immune cells is induced by cell surface receptors that stimulate store-operated calcium entry through calcium-release activated calcium (CRAC) channels. Since the...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2019.01.003
更新日期：2019-03-01 00:00:00
abstract：:The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0087
更新日期：2000-01-01 00:00:00
abstract：:There is growing evidence indicating that the pore structure of voltage-gated ion channels (VGICs) influences gating besides their conductance. Regarding low voltage-activated (LVA) Ca2+ channels, it has been demonstrated that substitutions of the pore aspartate (D) by a glutamate (D-to-E substitution) in domains III ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2020.102214
更新日期：2020-07-01 00:00:00
abstract：:To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(02)00111-2
更新日期：2002-07-01 00:00:00
abstract：:When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90008-i
更新日期：1990-04-01 00:00:00
abstract：:Stromal interaction molecule (STIM)-1 and -2 are multi-domain, single-pass transmembrane proteins involved in sensing changes in compartmentalized calcium (Ca2+) levels and transducing this cellular signal to Orai1 channel proteins. Our understanding of the molecular mechanisms underlying STIM signaling has been drama...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:We studied the contribution of store-operated or capacitative Ca2+-entry (SOCE or CCE, respectively) through store-operated Ca2+ channels (SOCCs) and the contribution of Ca2+-entry through receptor-operated, non-selective cation channels (ROCCs or NSCCs, respectively), on the M3-receptor-mediated (270 nM Ach) contract...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2005.06.035
更新日期：2005-11-01 00:00:00
abstract：:Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90074-4
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:Previous studies suggest that CaMKII activity is required for frequency-dependent acceleration of relaxation (FDAR) in ventricular myocytes. We propose that the underlying mechanism involves CaMKII-dependent regulation of myofilament Ca(2+) sensitivity. METHODS AND RESULTS:Cardiac function was measured in m...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.08.001
更新日期：2015-11-01 00:00:00
abstract：:Indo-1 is a new fluorescent indicator of the intracellular free calcium concentration Cai++. Indo-1 may be used in a similar manner as its predecessor quin2 but offers the principal advantage that the Ca++ saturated form of the Ca++ chelator has a emission maximum different in wavelength from that of free indo-1 (400 ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(86)90003-5
更新日期：1986-08-01 00:00:00
abstract：:Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.02.005
更新日期：2004-09-01 00:00:00
abstract：:The effect of palmitoyl-CoA (PCoA) on Ca2+ fluxes in unfractionated SR, longitudinal tubules (LSR) and terminal cisternae (TC) subfractions, obtained from rabbit fast-twitch skeletal muscles, was investigated. After MgATP-dependent Ca2+ preloading, PCoA released Ca2+ from unfractionated SR and TC, but not from LSR. Bo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90049-3
更新日期：1994-02-01 00:00:00
abstract：:Cultured human skeletal muscle cells are frequently used as a model to study muscle pathology, in which Ca2+ homeostasis might be affected. However, their excitation-contraction (E-C) coupling has been poorly investigated. In order to elucidate E-C coupling of cultured muscle cells, we activated the acetylcholine rece...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90099-3
更新日期：1997-01-01 00:00:00
abstract：:Studies of Ca2+ transport pathways in exocrine gland cells have been useful, chiefly because of the polarized nature of the secretory epithelial cells. In pancreatic acinar cells, for example, Ca2+ reloading of empty intracellular stores can occur solely via Ca2+ entry through the basal part of the plasma membrane. On...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00047-2
更新日期：2003-05-01 00:00:00
abstract：:Pituitary neuropeptide oxytocin is increasingly recognised as a cardiovascular hormone, in addition to its many regulatory roles in other organ systems. Studies in atrial and ventricular myocytes from the neonatal and adult rats have identified synthesis of oxytocin and the expression of oxytocin receptors in these ce...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102099
更新日期：2019-12-01 00:00:00
abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel which plays a major role in Ca2+ signalling. Three isoforms of IP3R have been identified (IP3R-1, IP3R-2 and IP3R-3) and most cell types express different proportions of each isoform. The differences between the p...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.01.002
更新日期：2007-09-01 00:00:00
abstract：:Cellular senescence is a stable cell proliferation arrest induced by a variety of stresses including telomere shortening, oncogene activation and oxidative stress. This process plays a crucial role in many physiopathological contexts, especially during aging when cellular senescence favors development of age-related d...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.04.001
更新日期：2018-03-01 00:00:00
abstract：:Here, we report a novel role for the cysteine protease calpain in store-operated calcium entry. Several structurally and mechanistically unrelated inhibitors of calpain inhibited Ca2+ entry activated in human platelets by thapsigargin-evoked Ca2+ store depletion or the physiological agonist thrombin, whereas inhibitor...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.05.008
更新日期：2007-02-01 00:00:00
