Abstract：
:The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by CaM. To gain insight into the differential regulation of cardiac and skeletal muscle RyRs by CaM, RyR1/RyR2 chimeras and mutants were expressed in HEK293 cells, and their single channel activities were measured using a lipid bilayer method. All RyR1/RyR2 chimeras and mutants were inhibited by CaM at 2μM Ca2+, consistent with CaM inhibition of RyR1 and RyR2 at micromolar Ca2+ concentrations. An RyR1/RyR2 chimera with RyR1 N-terminal amino acid residues (aa) 1-3725 and RyR2 C-terminal aa 3692-4968 were inhibited by CaM at <1μM Ca2+ similar to RyR2. In contrast, RyR1/RyR2 chimera with RyR1 aa 1-4301 and RyR2 4254-4968 was activated at <1μM Ca2+ similar to RyR1. Replacement of RyR1 aa 3726-4298 with corresponding residues from RyR2 conferred CaM inhibition at <1μM Ca2+, which suggests RyR1 aa 3726-4298 are required for activation by CaM. Characterization of additional RyR1/RyR2 chimeras and mutants in two predicted Ca2+ binding motifs in RyR1 aa 4081-4092 (EF1) and aa 4116-4127 (EF2) suggests that both EF-hand motifs and additional sequences in the large N-terminal regions are required for isoform-specific RyR1 and RyR2 regulation by CaM at submicromolar Ca2+ concentrations.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Xu L,Gomez AC,Pasek DA,Meissner G,Yamaguchi Ndoi
10.1016/j.ceca.2017.05.013subject
Has Abstractpub_date
2017-09-01 00:00:00pages
62-70eissn
0143-4160issn
1532-1991pii
S0143-4160(17)30055-6journal_volume
66pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Fluorescence imaging of calcium ions (Ca(2+)) has become an essential technique for investigation of signaling pathways involving Ca(2+) as a second messenger. But, Ca(2+) signaling is involved in many biological phenomena, and therefore simultaneous visualization of Ca(2+) and other biomolecules (multicolor imaging) ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2016.06.002
更新日期：2016-10-01 00:00:00
abstract：:Evidence is accumulating on a key role of T-type channels in neurotransmitter release. Recent works have brought undisputable proofs that T-type channels are capable of controlling hormone and neurotransmitters release in association with exocytosis of large dense-core and synaptic vesicles. T-type channel-secretion c...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.019
更新日期：2006-08-01 00:00:00
abstract：:The adenine nucleotide translocator (ANT) is an autoantigen in myocarditis and dilated cardiomyopathy. Carrier-specific antibodies impair myocardial energy metabolism and heart function. They cross-react with a myolemmal calcium channel and alter calcium fluxes in isolated myocytes. To test whether antibodies against ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0039
更新日期：1999-05-01 00:00:00
abstract：:Hepatocytes stimulated with calcium-mobilising agonists generate free Ca transients whose frequency is modulated by hormone concentration. Importantly, the time-course of individual free Ca transients is independent of agonist dose but does change with agonist species. A receptor-controlled model in which protein kina...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(91)90011-3
更新日期：1991-02-01 00:00:00
abstract：:Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.08.003
更新日期：2011-11-01 00:00:00
abstract：:We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca(2+) store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30 mM) 1-10.5 min after a 'priming' depolarisation pulse of 55 mM K(+) rev...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.06.007
更新日期：2007-03-01 00:00:00
abstract：:MAP kinases are a family of protein kinases that are ubiquitously expressed and play roles in most signal transduction pathways. They are activated within protein kinase cascades consisting of at least three kinases acting in series. In many, if not all cases, the three-kinase cascade, conveniently referred to as a MA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1054/ceca.1999.0074
更新日期：1999-11-01 00:00:00
abstract：:The L-type Ca2+ channel (Ca(v)1.2) is the main pathway for trans-sarcolemmal (SL) Ca2+ influx in cardiac myocytes. To maintain Ca2+ homeostasis, chronic SL Ca(2+)-influx must be matched by chronic SL efflux. In this study we tested the hypothesis that chronic downregulation of SL Ca2+ entry regulates SL extrusion. We ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.01.004
更新日期：2008-10-01 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellu...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.04.006
更新日期：2015-08-01 00:00:00
abstract：:The highly selective fluorescent Ca2+ indicator 'quin 2' has been loaded into ram and boar spermatozoa as the acetoxymethyl ester, 'quin 2/AM', which is hydrolysed and trapped in the cytoplasm. Loadings of several mM were not toxic to spermatozoa as judged by motility. Fluorescence measurements (mean +/- S.E.M.) indic...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(88)90037-1
更新日期：1988-02-01 00:00:00
abstract：:The rat parotid salivary gland shows marked alterations in phospholipid metabolism when stimulated by certain agonists. These agonists are those which cause cellular Ca mobilization by activation of muscarinic, alpha-adrenergic or peptidergic (substance P) receptors. The phospholipid changes apparently reflect the act...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(82)90024-0
更新日期：1982-10-01 00:00:00
abstract：:While genetically encoded Ca(2+) indicators (GECIs) allow Ca(2+) imaging in model organisms, the gene expression is often under the control of a single promoter that may drive expression beyond, the cell types of interest. To enable more cell-type specific targeting, GECIs can be brought under the, control of the inte...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.10.006
更新日期：2012-01-01 00:00:00
abstract：:Ca2+ pumps are essential for removing cytosolic Ca2+ either across the plasma membrane (PM) or into internal organelles such as the sarcoplasmic reticulum (SR). Four genes (PMCA1, PMCA2, PMCA3 and PMCA4) have been reported to encode the PM Ca2+ pumps and three (SERCA1, SERCA2 and SERCA3) to encode the SR Ca2+ pumps. T...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(92)90025-n
更新日期：1992-01-01 00:00:00
abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143416002001847
更新日期：2002-11-01 00:00:00
abstract：:Three decades ago, James W. Putney Jr. conceptualized the idea of store-operated calcium entry (SOCE) to explain how depletion of endoplasmic reticulum (ER) Ca2+ stores evokes Ca2+ influx across the plasma membrane. Since the publication of this highly influential idea, it is now established that SOCE is universal amo...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.12.006
更新日期：2017-05-01 00:00:00
abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00143-x
更新日期：2003-10-01 00:00:00
abstract：:Central to controlling intracellular calcium concentration ([Ca(2+)](i)) are a number of Ca(2+) transporters and channels with the L-type Ca(2+) channel, Na(+)-Ca(2+) exchanger and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) being of particular note in the heart. This review concentrates on the regulation of [Ca(2+)]...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2007.04.002
更新日期：2007-10-01 00:00:00
abstract：:Cytosolic Ca(2+) signals are fundamental for the early and late steps of myoblast differentiation and are, as in many cells, generated by Ca(2+) release from internal stores as well as by plasma membrane Ca(2+) entry. Our recent studies identified the store-operated Ca(2+) channels, Orai1 and TRPC1&C4, as crucial for ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Cytoplasmic calcium concentration was measured in primary cultured cells loaded with Fura-2. ET-1 (100 nM) caused c...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00085-x
更新日期：2003-09-01 00:00:00
abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90113-x
更新日期：1996-05-01 00:00:00
abstract：:The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.06.001
更新日期：2011-10-01 00:00:00
abstract：:The regulation of intracellular Ca(2+) signalling by phosphorylation processes remains poorly defined, particularly with regards to tyrosine phosphorylation. Evidence from non-excitable cells implicates tyrosine phosphorylation in the activation of so-called store-operated Ca(2+) channels (SOCCs), but their involvemen...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:A potential role of arachidonic acid in the modulation of insulin secretion was investigated by measuring its effects on calmodulin-dependent protein kinase and protein kinase C in islet subcellular fractions. The results were interpreted in the light of arachidonic acid effects on insulin secretion from intact islets...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90044-s
更新日期：1992-03-01 00:00:00
abstract：:Calcium-activated potassium channels are important in a variety of smooth muscles, contributing to excitability and contractility. In the myometrium previous work has focussed on the large conductance channels (BK), and the role of small conductance channels (SK) has received scant attention, despite the finding that ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.11.004
更新日期：2010-01-01 00:00:00
abstract：:Indo-1 is a new fluorescent indicator of the intracellular free calcium concentration Cai++. Indo-1 may be used in a similar manner as its predecessor quin2 but offers the principal advantage that the Ca++ saturated form of the Ca++ chelator has a emission maximum different in wavelength from that of free indo-1 (400 ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(86)90003-5
更新日期：1986-08-01 00:00:00
abstract：:Cellular redox status is maintained by the balance between series of antioxidant systems and production of reactive oxygen/nitrogenous species. Cells utilize this redox balance to mediate diverse physiological functions. Transient receptor potential (TRP) channels are non-selective cation channels that act as biosenso...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.02.009
更新日期：2016-08-01 00:00:00
abstract：:IP3R is located to the inner nuclear membrane. Nuclear IP3R is recognized as a 220 kD immunoreactive protein by antisera raised against purified rat brain IP3R. Antisera against C-terminal 95-108 peptide fragment derived from rat brain IP3R does not reveal immunoreactivity in the nucleus. Nuclear IP3R is sensitive to ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90094-9
更新日期：1994-10-01 00:00:00
abstract：:The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90013-0
更新日期：1997-09-01 00:00:00
abstract：:To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90081-0
更新日期：1996-02-01 00:00:00
abstract：:A procedure for calibration of fluorescence signals from adult rat heart cells loaded with the -AM ester of fura-2 is described. Calibration is complicated by dye compartmentation and potentially incomplete dye hydrolysis. These problems were overcome by subtracting from fluorescence transients the non-cytosolic (mito...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90050-1
更新日期：1998-10-01 00:00:00
