Abstract：
:Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellular mechanisms responsible for impaired mitochondrial metabolism in dystrophic cardiomyopathy at early stages of the disease. We employed electrophysiological and imaging techniques to study mitochondrial structure and function in cardiomyocytes from mdx mice, an animal model of DMD. Here we show that mitochondrial matrix was progressively oxidized in myocytes isolated from mdx mice. Moreover, an abrupt increase in workload resulted in significantly more pronounced oxidation of mitochondria in dystrophic cells. Electron micrographs revealed a gradually increased number of damaged mitochondria in mdx myocytes. Degradation in mitochondrial structure was correlated with progressive increase in mitochondrial Ca(2+) sequestration and mitochondrial depolarization, despite a substantial and persistent elevation in resting cytosolic sodium levels. Treatment of mdx cells with cyclosporine A, an inhibitor of mitochondrial permeability transition pore (mPTP), shifted both resting and workload-dependent mitochondrial redox state to the levels recorded in control myocytes. It also significantly reduced workload dependent depolarization of mitochondrial membrane in dystrophic cardiomyocytes. Overall, our studies highlight age dependent deterioration of mitochondrial function in dystrophic cardiomyocytes, which seems to be associated with excessive opening of mPTP due to oxidative stress and cellular Ca(2+) overload.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Kyrychenko V,Poláková E,Janíček R,Shirokova Ndoi
10.1016/j.ceca.2015.04.006subject
Has Abstractpub_date
2015-08-01 00:00:00pages
186-95issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(15)00077-9journal_volume
58pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Cellular senescence is a stable cell proliferation arrest induced by a variety of stresses including telomere shortening, oncogene activation and oxidative stress. This process plays a crucial role in many physiopathological contexts, especially during aging when cellular senescence favors development of age-related d...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.04.001
更新日期：2018-03-01 00:00:00
abstract：:The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(82)90004-5
更新日期：1982-08-01 00:00:00
abstract：:We previously found in single channel studies that ryanodine receptor (RyR) channel activity can be made insensitive to block by Mg2+ when terminal cisternae of sarcoplasmic reticulum, incorporated into planar bilayers, are treated with protein kinase A (PKA) or Ca2+/calmodulin dependent protein kinase type II (CamPK ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90064-0
更新日期：1995-09-01 00:00:00
abstract：:The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.03.004
更新日期：2014-07-01 00:00:00
abstract：:The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90068-3
更新日期：1997-10-01 00:00:00
abstract：:The ability of the ectodermal cells to be induced and to differentiate toward neural tissue, called neural competence, is acquired shortly before gastrulation and lost during late gastrula stages in Pleurodeles waltl embryos. We have examined ectodermal cells' neural competence in relation to the evolution of the dens...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90036-5
更新日期：1995-03-01 00:00:00
abstract：:Secretory granules of pancreatic β-cells contain high concentrations of Ca2+ ions that are co-released with insulin in the extracellular milieu upon activation of exocytosis. As a consequence, an increase in the extracellular Ca2+ concentration ([Ca2+]ext) in the microenvironment immediately surrounding β-cells should...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.01.002
更新日期：2012-05-01 00:00:00
abstract：:Receptors for extracellular nucleotides (the P2X-calcium channels and the phospholipase C-coupled P2Y receptors) play key roles in pain signaling, but little is known on their function in trigeminal ganglia, whose hyperactivation leads to the development of migraine pain. Here we characterize calcium signaling via P2X...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.10.003
更新日期：2008-06-01 00:00:00
abstract：:The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.12.009
更新日期：2019-03-01 00:00:00
abstract：:An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90070-5
更新日期：1989-04-01 00:00:00
abstract：:MAP kinases are a family of protein kinases that are ubiquitously expressed and play roles in most signal transduction pathways. They are activated within protein kinase cascades consisting of at least three kinases acting in series. In many, if not all cases, the three-kinase cascade, conveniently referred to as a MA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1054/ceca.1999.0074
更新日期：1999-11-01 00:00:00
abstract：:Store-operated calcium entry (SOCE) is activated within the endoplasmic reticulum (ER)-plasma membrane (PM) junctions in the plasma membrane and involves assembly of the channels, Orai1 and TRPC1, with the regulatory protein, STIM1, in response to depletion of Ca(2+) in the ER. The dynamic assembly of the channel comp...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2015.03.008
更新日期：2015-10-01 00:00:00
abstract：:Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...
journal_title：Cell calcium
pub_type： 评论,杂志文章
doi：10.1016/j.ceca.2018.11.001
更新日期：2018-12-01 00:00:00
abstract：:Pacemaker cells residing in the sinoatrial node generate the regular heartbeat. Ca2+ signaling controls the heartbeat rate-directly, through the effect on membrane molecules (NCX exchange, K+ channel), and indirectly, through activation of calmodulin-AC-cAMP-PKA signaling. Thus, the physiological role of signaling in ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.12.008
更新日期：2019-03-01 00:00:00
abstract：:Factors contributing to "local control" of Ca2+ release in cardiac myocytes are incompletely understood. We induced local release of Ca2+ by regional exposure of mouse atrial and ventricular myocytes to 10mM caffeine for 500 ms using a rapid solution switcher. Propagation of Ca2+ release was imaged by means of a Nipko...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2005.03.004
更新日期：2005-07-01 00:00:00
abstract：:The endoplasmic reticulum (ER) acts as a dynamic calcium store and is involved in the generation of specific patterns of calcium signals in neurons. Calcium is mobilized from the ER store by multiple signaling cascades, and neuronal activity is known to regulate ER calcium levels. We asked how neurons regulate ER calc...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.04.002
更新日期：2015-08-01 00:00:00
abstract：:The lungs as the gateways of our body to the external environment are essential for gas exchange. They are also exposed to toxicants from two sides, the airways and the vasculature. Apart from naturally produced toxic agents, millions of human made chemicals were produced since the beginning of the industrial revoluti...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.04.005
更新日期：2017-11-01 00:00:00
abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90004-e
更新日期：1990-04-01 00:00:00
abstract：:K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ +...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.06.002
更新日期：2018-09-01 00:00:00
abstract：:Cooling can induce Ca(2+) signaling via activation of temperature-sensitive ion channels such as TRPM8, TRPA1 and ryanodine receptor channels. Here we have studied the mechanism of cooling-evoked Ca(2+) signaling in mouse olfactory ensheathing cells (OECs), a specialized type of glial cells in the olfactory nerve laye...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.04.017
更新日期：2012-08-01 00:00:00
abstract：:The A23187 induced calcium uptake in ATP depleted cells was determined at pH 6.9 in the presence of trifluoperazine (TFP, 0.30 mM), compound 48/80 (0.89 mg/ml), 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8, 2.13 mM) and verapamil (1.81 mM). Apart from verapamil the drugs all increased the maximum rate of ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(84)90022-8
更新日期：1984-10-01 00:00:00
abstract：:Many fluorescent Ca indicators, particularly those loaded as acetoxymethyl (AM)-ester derivatives, are known to become compartmentalized into organelles. This property can be exploited to measure changes in free [Ca] in subcellular compartments, including the inositol (1,4,5)-trisphosphate-sensitive store. However, qu...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90029-9
更新日期：1996-09-01 00:00:00
abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143416002001847
更新日期：2002-11-01 00:00:00
abstract：:Endothelin-1 (ET-1), a potent vasoactive peptide with a pathogenic role in vascular diseases, has been shown to induce the activation of ERK1/2, PKB and the expression of a transcriptional regulator, the early growth response 1 (Egr-1), key mediators of hypertrophic and proliferative responses in vascular smooth muscl...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.10.002
更新日期：2013-12-01 00:00:00
abstract：:Receptor-activated cytoplasmic calcium (Ca2+) oscillations have been investigated in single pancreatic acinar cells by microfluorimetry (Fura-2 as indicator). At submaximal concentrations of the agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8), both give rise to oscillatory changes in the cytosolic...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(91)90016-8
更新日期：1991-02-01 00:00:00
abstract：:Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2005.01.018
更新日期：2005-05-01 00:00:00
abstract：:Middle East Respiratory Syndrome coronavirus (MERS-CoV) infections are associated with a significant mortality rate, and existing drugs show poor efficacy. Identifying novel targets/pathways required for MERS infectivity is therefore important for developing novel therapeutics. As an enveloped virus, translocation thr...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.08.003
更新日期：2018-11-01 00:00:00
abstract：:In the cochlea, cell damage triggers intercellular Ca2+ waves that propagate through the glial-like supporting cells that surround receptor hair cells. These Ca2+ waves are thought to convey information about sensory hair cell-damage to the surrounding supporting cells within the cochlear epithelium. Mitochondria are ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.06.005
更新日期：2009-08-01 00:00:00
abstract：:Fura-2 and BAPTA were previously shown to be competitive antagonists of inositol trisphosphate (InsP3) receptors, but for practical reasons the analyses were performed at pH 8.3. We recently developed a scintillation proximity assay (SPA) for pure cerebellar InsP3 receptors which allows low affinity interactions to be...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1998.0016
更新日期：1999-02-01 00:00:00
abstract：:The calcium release channel/ryanodine receptor of rabbit skeletal muscle sarcoplasmic reticulum is tightly associated with the immunophilin FK-506 binding protein (FKBP-12). The immunosuppressant drug FK-506 effectively dissociates FKBP-12 from the calcium release channel of terminal cisternae (TC) vesicles. Furthermo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90048-5
更新日期：1994-02-01 00:00:00
