Abstract：
:While genetically encoded Ca(2+) indicators (GECIs) allow Ca(2+) imaging in model organisms, the gene expression is often under the control of a single promoter that may drive expression beyond, the cell types of interest. To enable more cell-type specific targeting, GECIs can be brought under the, control of the intersecting expression from two promoters. Here, we present the splitting and, reassembly of two representative GECIs (TN-XL and GCaMP2) mediated by the split intein from Nostoc, punctiforme (NpuDnaE). While the split TN-XL biosensor offered ratiometric Ca(2+) imaging, it had a, diminished Ca(2+) response relative to the native TN-XL biosensor. In contrast, the split GCaMP2, biosensor retained similar Ca(2+) response to the native GCaMP2. The split GCaMP2 biosensor was, further targeted to the pharyngeal muscles of Caenorhabditis elegans where Ca(2+) signals from feeding C. elegans, were imaged. Thus, we envision that increased cell-type targetability of GECIs is feasible with two, complementary promoters.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Wong SS,Kotera I,Mills E,Suzuki H,Truong Kdoi
10.1016/j.ceca.2011.10.006subject
Has Abstractpub_date
2012-01-01 00:00:00pages
57-64issue
1eissn
0143-4160issn
1532-1991pii
S0143-4160(11)00203-Xjournal_volume
51pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Peripheral astrocytic processes (PAPs) are highly motile structures that are strategically positioned in close proximity to synapses. Long-lasting PAP retraction in hypothalamus is known to alter synaptic transmission. The PAP motility is likely to be actin-based because they are known to contain actin-related protein...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.03.001
更新日期：2013-05-01 00:00:00
abstract：:Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.11.001
更新日期：2012-03-01 00:00:00
abstract：:Calbindin D28K (formerly known as vitamin D-dependent calcium binding protein and referred to here as calbindin) is found in a wide variety of tissues, but only in certain cells within those tissues. Apart from its ability to bind calcium, nothing is known about its function in these cells. To investigate its role we ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90055-b
更新日期：1993-04-01 00:00:00
abstract：:Cytosolic Ca(2+) signals are fundamental for the early and late steps of myoblast differentiation and are, as in many cells, generated by Ca(2+) release from internal stores as well as by plasma membrane Ca(2+) entry. Our recent studies identified the store-operated Ca(2+) channels, Orai1 and TRPC1&C4, as crucial for ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:Studies were conducted using normal and human papillomavirus Type 18 (HPV-18) immortalized human keratinocytes to assess possible alterations in the differentiation process as a consequence of increased intracellular calcium concentration. Normal keratinocytes exposed to increased extracellular calcium or the phorbol ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(91)90050-o
更新日期：1991-05-01 00:00:00
abstract：:For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(85)90008-9
更新日期：1985-06-01 00:00:00
abstract：:Several proteins have been identified that associate with calcium release channels and potentially regulate their function. Using tacrolimus as a pharmacological tool, we investigated whether the immunophilin FKBP12 modulates ryanodine receptor channels in intestinal smooth muscle. Results with PCR demonstrated the pr...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90078-6
更新日期：1997-12-01 00:00:00
abstract：:Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90007-s
更新日期：1993-06-01 00:00:00
abstract：:This work shows, for the first time, a properly metabolically regulated squid nerve Na(+)/Ca(2+) exchanger (NCXSQ1) heterologous expressed in Saccharomyces cerevisiae. The exchanger was fused to the enhanced green fluorescence protein (eGFP) on its C-terminus and had two tags, a Strep-tag II and 6 histidines, added to...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.03.009
更新日期：2009-05-01 00:00:00
abstract：:We report here the first local and global Ca(2+) measurements made from in situ terminal arterioles. The advantages of the method are that there is minimal disturbance to the vessels, which retain their relationship to the tissue they are supplying (rat ureter) and the small size of vessel that can be studied. Good lo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00019-8
更新日期：2003-07-01 00:00:00
abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2005.06.012
更新日期：2005-09-01 00:00:00
abstract：:The importance of Ca2+-signaling for many subcellular processes is well established in higher eukaryotes, whereas information about protozoa is restricted. Recent genome analyses have stimulated such work also with Alveolates, such as ciliates (Paramecium, Tetrahymena) and their pathogenic close relatives, the Apicomp...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.01.006
更新日期：2012-05-01 00:00:00
abstract：:Dynamic nuclear Ca(2+) signals play pivotal roles in diverse cellular functions including gene transcription, cell growth, differentiation, and apoptosis. Here we report a novel nuclear Ca(2+) regulatory mechanism mediated by inositol 1,4,5-trisphosphate receptors (IP(3)Rs) around the nucleus in developing cardiac myo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.04.017
更新日期：2008-02-01 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons. Mutations in Cu/Zn superoxide dismutase-1 (SOD1) cause familial ALS but the molecular mechanisms whereby these mutations induce motor neuron death remain controversial. Here, we show that stable overex...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.02.004
更新日期：2011-04-01 00:00:00
abstract：:The mechanisms that regulate ion and fluid transport by the human intrahepatic bile duct have not been well defined. Human intrahepatic biliary cell lines that we have developed were used to identify and characterize purinoceptors based on increases in intracellular calcium in response to ATP and other nucleotides. In...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90111-6
更新日期：1995-05-01 00:00:00
abstract：:TRPM family (Transient receptor potential channels, M for melastatin) is a group of intrinsic plasma membrane ion channels which are widely expressed throughout human body. It has been identified as a potent entry point of working desperate diseases out in a new way with newfangled ideas and safer technological means....
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2019.02.011
更新日期：2019-05-01 00:00:00
abstract：:Store-operated calcium entry (SOCE) is activated within the endoplasmic reticulum (ER)-plasma membrane (PM) junctions in the plasma membrane and involves assembly of the channels, Orai1 and TRPC1, with the regulatory protein, STIM1, in response to depletion of Ca(2+) in the ER. The dynamic assembly of the channel comp...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2015.03.008
更新日期：2015-10-01 00:00:00
abstract：:The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(83)90024-6
更新日期：1983-12-01 00:00:00
abstract：:Receptors for extracellular nucleotides (the P2X-calcium channels and the phospholipase C-coupled P2Y receptors) play key roles in pain signaling, but little is known on their function in trigeminal ganglia, whose hyperactivation leads to the development of migraine pain. Here we characterize calcium signaling via P2X...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.10.003
更新日期：2008-06-01 00:00:00
abstract：:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2001.0233
更新日期：2001-10-01 00:00:00
abstract：:In salivary acinar cells, muscarinic-induced fluid secretion is associated with a 1,4,5-IP3 induced increase in the cytosolic free Ca2+ concentration ([Ca2+]i), which in turn activates Ca(2+)-dependent K+ and Cl- channels that modulate the membrane potential. In the present study the influence of the membrane potentia...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90076-i
更新日期：1993-07-01 00:00:00
abstract：:We previously found in single channel studies that ryanodine receptor (RyR) channel activity can be made insensitive to block by Mg2+ when terminal cisternae of sarcoplasmic reticulum, incorporated into planar bilayers, are treated with protein kinase A (PKA) or Ca2+/calmodulin dependent protein kinase type II (CamPK ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90064-0
更新日期：1995-09-01 00:00:00
abstract：:CatSper is a sperm-specific Ca(2+) channel that plays an essential role in the male fertility. However, its biophysical properties have been poorly characterized mainly due to its deficient heterologous expression. As other voltage-gated Ca(2+) channels (CaVs), CatSper possesses a conserved Ca(2+)-selective filter mot...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2016.03.011
更新日期：2016-07-01 00:00:00
abstract：:The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity i...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(88)90022-x
更新日期：1988-08-01 00:00:00
abstract：:The concentration of free Ca2+ in the cytoplasm and organelles of individual mouse pancreatic beta-cells was estimated with dual wavelength microfluorometry and the indicators Fura-2 and furaptra. Measuring the increase of cytoplasmic Ca2+ resulting from intracellular mobilization of the ion in ob/ob mouse beta-cells,...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0087
更新日期：2000-01-01 00:00:00
abstract：:Agonist induced increases in intracellular free calcium, [Ca2+]i, were measured in single Fura-2 loaded bovine aortic endothelial (BAE) cells by dual wavelength microspectrofluorimetry. Low doses of ATP (less than 10 microM) induced complex changes in [Ca2+]i. These changes usually consisted of a large initial transie...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90011-g
更新日期：1992-04-01 00:00:00
abstract：:Using the low-affinity fluorescent Ca(2+) indicators, Mag-Fura-2 and Mag-Fura Red, we studied light- and InsP(3)-induced Ca(2+) release in permeabilized microvillar photoreceptors of the medicinal leech, Hirudo medicinalis. Two major components of the phosphoinositide signaling pathway, phospholipase-C and the InsP(3)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2001.0195
更新日期：2001-05-01 00:00:00
abstract：:Specific sequences (cis-acting elements) in the 3'-untranslated region (UTR) of RNA, together with stabilizing and destabilizing proteins (trans-acting factors), determine the mRNA stability, and consequently, the level of expression of several proteins. Such interactions were discovered initially for short-lived mRNA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.004
更新日期：2006-10-01 00:00:00
abstract：:One of the important events during hypoxia or ischemia in the brain (or other organs for that matter, including the myocardium) is the accumulation of Ca2+ ions intracellularly. Although various studies have shown various sources of and routes for Ca2+ entry and accumulation, it is clear now that it is likely that the...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.02.013
更新日期：2004-09-01 00:00:00
abstract：:An important challenge in the study of Ca2+ signalling is to understand the dynamics of intracellular Ca2+ levels during and after physiological stimulation. While extensive information is available regarding the structural and biophysical properties of Ca2+ channels, pumps and exchangers that control cellular Ca2+ mo...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1054/ceca.2000.0172
更新日期：2000-11-01 00:00:00
