Independent and interrelated regulation of ornithine decarboxylase by calcium and cAMP in fetal rat osteoblasts.


:The present study was undertaken to determine in fetal rat osteoblasts whether and how the intracellular messengers calcium and cAMP are involved in stimulation of ornithine decarboxylase (ODC) activity. For that purpose we used different drugs affecting [Ca2+]i and cAMP concentration. A23187 stimulates ODC activity in a biphasic way, with maximal stimulation at 100 nM A23187. At that concentration no stimulation of cAMP production was observed. Basal and A23187-stimulated (100 nM) ODC activity were inhibited by EGTA and trifluoperazine. Forskolin stimulated dose-dependently both ODC activity and cAMP production. Besides these effects forskolin (1 and 10 microM) increased the [Ca2+]i via an increased calcium influx. Addition of La3+, verapamil or EGTA, but not of trifluoperazine, significantly inhibited the forskolin-stimulated (10 microM) ODC activity. When forskolin (100 nM and 1 microM) was added together with 1 microM A23187, a synergistic stimulation of ODC activity was observed. These results implicate that calcium is involved in basal ODC activity, and that ODC activity can be stimulated via (1) a cAMP-independent calcium pathway, and (2) a calcium-dependent, cAMP pathway. It is proposed that ODC activity can be stimulated via interaction between calcium and cAMP.


Cell Calcium


Cell calcium


van Leeuwen JP,Bos MP,Herrmann-Erlee MP




Has Abstract


1988-08-01 00:00:00














  • Classes and mechanisms of calcium waves.

    abstract::The best known calcium waves move at about 5-30 microns/s (at 20 degrees C) and will be called fast waves to distinguish them from slow (contractile) ones which move at 0.1-1 microns/s as well as electrically propagated, ultrafast ones. Fast waves move deep within cells and seem to underlie most calcium signals. Their...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Jaffe LF

    更新日期:1993-11-01 00:00:00

  • Ion channels in smooth muscle: regulation by the sarcoplasmic reticulum and mitochondria.

    abstract::In smooth muscle, Ca(2+) regulates cell division, growth and cell death as well as providing the main trigger for contraction. Ion channels provide the major access route to elevate the cytoplasmic Ca(2+) concentration ([Ca(2+)](c)) in smooth muscle by permitting Ca(2+) entry across the plasma membrane and release of ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Chalmers S,Olson ML,MacMillan D,Rainbow RD,McCarron JG

    更新日期:2007-10-01 00:00:00

  • Calcium dependence of bleb formation and cell death in hepatocytes.

    abstract::Calcium dependence of bleb formation and cell death was evaluated in rat hepatocytes following ATP depletion by metabolic inhibition with KCN and iodoacetate ('chemical hypoxia'). Cytosolic free Ca2+ was measured in single cells by ratio imaging of Fura-2 fluorescence using multiparameter digitized video microscopy. C...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Nieminen AL,Gores GJ,Wray BE,Tanaka Y,Herman B,Lemasters JJ

    更新日期:1988-12-01 00:00:00

  • T-type Ca2+ channels and absence epilepsy.

    abstract::Burst firing of the thalamic neurons is driven by the low threshold Ca2+ spike generated by Ca2+ influx through T-type Ca2+ channels when these channels are activated by membrane hyperpolarization due to inhibitory inputs. The major inhibitory inputs to the thalamocortical (TC) neurons are from the GABAergic neurons i...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Shin HS

    更新日期:2006-08-01 00:00:00

  • Inositol 1,4,5-trisphosphate binding sites copurify with the putative Ca-storage protein calreticulin in rat liver.

    abstract::Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Enyedi P,Szabadkai G,Krause KH,Lew DP,Spät A

    更新日期:1993-06-01 00:00:00

  • Role of STIM and Orai proteins in the store-operated calcium signaling pathway.

    abstract::Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Hewavitharana T,Deng X,Soboloff J,Gill DL

    更新日期:2007-08-01 00:00:00

  • Pancreastatin inhibits insulin secretion in RINm5F cells through obstruction of G-protein mediated, calcium-directed exocytosis.

    abstract::To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Hertelendy ZI,Patel DG,Knittel JJ

    更新日期:1996-02-01 00:00:00

  • The annexin 2-S100A10 complex and its association with TRPV6 is regulated by cAMP/PKA/CnA in airway and gut epithelia.

    abstract::The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independe...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Borthwick LA,Neal A,Hobson L,Gerke V,Robson L,Muimo R

    更新日期:2008-08-01 00:00:00

  • Skeletal muscle excitation-contraction coupling is independent of a conserved heptad repeat motif in the C-terminus of the DHPRbeta(1a) subunit.

    abstract::In skeletal muscle excitation-contraction (EC) coupling the sarcolemmal L-type Ca(2+) channel or 1,4-dihydropyridine receptor (DHPR) transduces the membrane depolarization signal to the sarcoplasmic Ca(2+) release channel RyR1 via protein-protein interaction. While it is evident that the pore-forming and voltage-sensi...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Dayal A,Schredelseker J,Franzini-Armstrong C,Grabner M

    更新日期:2010-06-01 00:00:00

  • Nuclear Ca2+ sparks and waves mediated by inositol 1,4,5-trisphosphate receptors in neonatal rat cardiomyocytes.

    abstract::Dynamic nuclear Ca(2+) signals play pivotal roles in diverse cellular functions including gene transcription, cell growth, differentiation, and apoptosis. Here we report a novel nuclear Ca(2+) regulatory mechanism mediated by inositol 1,4,5-trisphosphate receptors (IP(3)Rs) around the nucleus in developing cardiac myo...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Luo D,Yang D,Lan X,Li K,Li X,Chen J,Zhang Y,Xiao RP,Han Q,Cheng H

    更新日期:2008-02-01 00:00:00

  • Control of photoreceptor proteins by Ca2+.

    abstract::A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Koch KW

    更新日期:1995-10-01 00:00:00

  • Agonist mobilization of sarcoplasmic reticular calcium in smooth muscle: functional coupling to the plasmalemmal Na+/Ca2+ exchanger?

    abstract::There is a close association of peripheral sarcoplasmic reticulum (SR), containing IP3 receptors, and regions of the plasma membrane enriched in the Na+/Ca2+ exchanger in smooth muscle. We have tested the possibility in rat uterine smooth muscle that Ca2+ released from the SR is preferentially removed from the cytosol...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Taggart MJ,Wray S

    更新日期:1997-11-01 00:00:00

  • Ca-pumps in smooth muscle: one in plasma membrane and another in endoplasmic reticulum.

    abstract::For several years it has been debated whether the Ca-pump in smooth muscle is located in the plasma membrane or in the endoplasmic reticulum (alias sarcoplasmic reticulum). Experimental evidence using skinned smooth muscle cells and subcellular membrane fractions isolated from a number of smooth muscles is reviewed he...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Grover AK

    更新日期:1985-06-01 00:00:00

  • Role of calcium in the regulation of sugar transport in the avian erythrocyte: effects of the calcium ionophore, A23187.

    abstract::The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Bihler I,Charles P,Sawh PC

    更新日期:1982-08-01 00:00:00

  • Calcium transport mechanisms in dog red blood cells studied from measurements of initial flux rates.

    abstract::Ca2+ efflux from dog red blood cells loaded with Ca2+ using the A23187 ionophore could be separated into two main components: (1) Mg- and ATP-dependent (active transport) and (2) dependent on external Na (K1/2 around 15 mM); at 80 microM internal free Ca the relative magnitudes of these fluxes were 70% and 30% respect...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Altamirano AA,Beaugé L

    更新日期:1985-12-01 00:00:00

  • Chronic fluoxetine administration increases expression of the L-channel gene Cav1.2 in astrocytes from the brain of treated mice and in culture and augments K(+)-induced increase in [Ca(2+)]i.

    abstract::We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Du T,Liang C,Li B,Hertz L,Peng L

    更新日期:2014-03-01 00:00:00

  • The effect of endotoxemia on concanavalin A induced alterations in cytoplasmic free calcium in rat spleen cells as determined with Fluo-3.

    abstract::The effects of acute (3 h) and chronic (30 h) in vivo infusions of Escherichia coli endotoxin on the Ca2+ homeostasis of rat spleen cells was investigated. Conditions were established for obtaining reliable estimates of [Ca2+]i in these cells using the newly-developed Ca2+ indicator Fluo-3. The resting [Ca2+]i of sple...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Hagar AF,Spitzer JA

    更新日期:1992-02-01 00:00:00

  • Dynamics of intracellular calcium in hair cells isolated from the semicircular canal of the frog.

    abstract::Changes in cytosolic free Ca(2+) concentration ([Ca(2+)]i) were monitored optically in hair cells mechanically isolated from frog semicircular canals using the membrane-impermeant form of the Ca(2+)-selective dye Oregon Green 488 BAPTA-1 (OG, 100 microM). Cells stimulated by depolarization under whole-cell voltage cla...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Rispoli G,Martini M,Rossi ML,Mammano F

    更新日期:2001-08-01 00:00:00

  • The aging mouse brain: cognition, connectivity and calcium.

    abstract::Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Radulescu CI,Cerar V,Haslehurst P,Kopanitsa M,Barnes SJ

    更新日期:2021-01-23 00:00:00

  • cAMP and Ca²⁺ signaling in secretory epithelia: crosstalk and synergism.

    abstract::The Ca(2+) and cAMP/PKA pathways are the primary signaling systems in secretory epithelia that control virtually all secretory gland functions. Interaction and crosstalk in Ca(2+) and cAMP signaling occur at multiple levels to control and tune the activity of each other. Physiologically, Ca(2+) and cAMP signaling oper...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Ahuja M,Jha A,Maléth J,Park S,Muallem S

    更新日期:2014-06-01 00:00:00

  • Inositol lipids and cell stimulation in mammalian salivary gland.

    abstract::The rat parotid salivary gland shows marked alterations in phospholipid metabolism when stimulated by certain agonists. These agonists are those which cause cellular Ca mobilization by activation of muscarinic, alpha-adrenergic or peptidergic (substance P) receptors. The phospholipid changes apparently reflect the act...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Putney JW Jr

    更新日期:1982-10-01 00:00:00

  • A role for the intracellular protease calpain in the activation of store-operated calcium entry in human platelets.

    abstract::Here, we report a novel role for the cysteine protease calpain in store-operated calcium entry. Several structurally and mechanistically unrelated inhibitors of calpain inhibited Ca2+ entry activated in human platelets by thapsigargin-evoked Ca2+ store depletion or the physiological agonist thrombin, whereas inhibitor...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Harper AG,Sage SO

    更新日期:2007-02-01 00:00:00

  • Capacitative Ca2+ entry (CCE) induced by luminal and basolateral ATP in polarised MDCK-C7 cells is restricted to the basolateral membrane.

    abstract::In previous studies we have characterised various properties of capacitative Ca2+ entry (CCE) in different epithelia. After Ca2+ store depletion with PLC/InsP3-coupled agonists or by inhibition of store Ca2+ uptake, with for example thapsigargin, Ca2+ influx is activated. This leads to a sustained cellular response (e...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Gordjani N,Nitschke R,Greger R,Leipziger J

    更新日期:1997-08-01 00:00:00

  • Examination of the role of TRPM8 in human mast cell activation and its relevance to the etiology of cold-induced urticaria.

    abstract::Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Medic N,Desai A,Komarow H,Burch LH,Bandara G,Beaven MA,Metcalfe DD,Gilfillan AM

    更新日期:2011-11-01 00:00:00

  • Tetracaine stimulates insulin secretion from the pancreatic beta-cell by release of intracellular calcium.

    abstract::The role of intracellular calcium stores in stimulus-secretion coupling in the pancreatic beta-cell is largely unknown. We report here that tetracaine stimulates insulin secretion from collagenase-isolated mouse islets of Langerhans in the absence of glucose or extracellular calcium. We also found that the anesthetic ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Mears D,Leighton X,Atwater I,Rojas E

    更新日期:1999-01-01 00:00:00

  • Effects of redox agents on the Ca2+-activated K+ channel.

    abstract::The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: García-Sancho J,Herreros B

    更新日期:1983-12-01 00:00:00

  • PPADS is a reversible competitive antagonist of the NAADP receptor.

    abstract::NAADP has been shown to act as a second messenger in a wide range of systems from plants to mammalian cells. Although it had always been considered as a canonical second messenger, recent work has shown that it is also active when applied extracellularly. It has also been suggested that NAADP might have a direct actio...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Billington RA,Genazzani AA

    更新日期:2007-06-01 00:00:00

  • Inhibition of calmodulin-dependent and independent cardiac sarcoplasmic reticulum activities by R24571.

    abstract::R24571 a derivative of the antimycotic miconazole, appears to be 5 to 8 times more potent than trifluoperazine in its ability to inhibit the calmodulin-dependent phosphorylation of cardiac sarcoplasmic reticulum vesicles. The cAMP-dependent protein kinase mediated phosphorylation of cardiac sarcoplasmic reticulum was ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Louis CF,Turnquist J,Jarvis B

    更新日期:1983-04-01 00:00:00

  • Control of protein expression through mRNA stability in calcium signalling.

    abstract::Specific sequences (cis-acting elements) in the 3'-untranslated region (UTR) of RNA, together with stabilizing and destabilizing proteins (trans-acting factors), determine the mRNA stability, and consequently, the level of expression of several proteins. Such interactions were discovered initially for short-lived mRNA...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Misquitta CM,Chen T,Grover AK

    更新日期:2006-10-01 00:00:00

  • Localized calcium signalling and neuronal integration in cerebellar Purkinje neurones.

    abstract::The use of high resolution imaging techniques has revealed new forms of dendritic signal integration in neurones. In contrast to electrical signals that have a more widespread influence on the cell, brief Ca2+ transients resulting from synaptic activation are often restricted to a small part of the dendritic tree. In ...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Eilers J,Plant T,Konnerth A

    更新日期:1996-08-01 00:00:00