Abstract:
:CatSper is a sperm-specific Ca(2+) channel that plays an essential role in the male fertility. However, its biophysical properties have been poorly characterized mainly due to its deficient heterologous expression. As other voltage-gated Ca(2+) channels (CaVs), CatSper possesses a conserved Ca(2+)-selective filter motif ([T/S]x[D/E]xW) in the pore region. Interestingly, CatSper conserves four aspartic acids (DDDD) as the negatively charged residues in this motif while high voltage-activated CaVs have four glutamic acids (EEEE) and low voltage-activated CaVs possess two glutamic acids and two aspartic acids (EEDD). Previous studies based on site-directed mutagenesis of L- and T-type channels showed that the number of D seems to have a negative correlation with their cadmium (Cd(2+)) sensitivity. These results suggest that CatSper (DDDD) would have low sensitivity to Cd(2+). To explore Cd(2+)-sensitivity and -permeability of CatSper, we performed two types of experiments: 1) Electrophysiological analysis of heterologously expressed human CaV3.1 channel and three pore mutants (DEDD, EDDD and DDDD), 2) Cd(2+) imaging of human spermatozoa with FluoZin-1. Electrophysiological studies showed a significant increase in Cd(2+) and manganese (Mn(2+)) currents through the CaV3.1 mutants as well as a reduction in the inhibitory effect of Cd(2+) on the Ca(2+) current. In fluorescence imaging with human sperm, we observed an increase in Cd(2+) influx potentiated by progesterone, a potent activator of CatSper. These results support our hypothesis, namely that Cd(2+)-sensitivity and -permeability are related to the absolute number of D in the Ca(2+)-selective filter independently to the type of the Cav channels.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Garza-López E,Chávez JC,Santana-Calvo C,López-González I,Nishigaki Tdoi
10.1016/j.ceca.2016.03.011subject
Has Abstractpub_date
2016-07-01 00:00:00pages
41-50issue
1eissn
0143-4160issn
1532-1991pii
S0143-4160(16)30037-9journal_volume
60pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.06.002
更新日期:2008-03-01 00:00:00
abstract::Dopamine is one of the key neurotransmitters in the central nervous system and plays an important role in physiological processes, as well as in the development of many diseases. Here we report a receptor-independent signalling pathway induced by dopamine in CNS neurons. In cultured neurons from midbrain, cortex and h...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2010.08.008
更新日期:2010-08-01 00:00:00
abstract::Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90033-6
更新日期:1997-02-01 00:00:00
abstract::The lungs as the gateways of our body to the external environment are essential for gas exchange. They are also exposed to toxicants from two sides, the airways and the vasculature. Apart from naturally produced toxic agents, millions of human made chemicals were produced since the beginning of the industrial revoluti...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2017.04.005
更新日期:2017-11-01 00:00:00
abstract::In many animals, a rise in intracellular calcium levels is the trigger for egg activation, the process by which an arrested mature oocyte transitions to prepare for embryogenesis. In nearly all animals studied to date, this calcium rise, and thus egg activation, is triggered by the fertilizing sperm. However in the in...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2012.11.008
更新日期:2013-01-01 00:00:00
abstract::Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00082-4
更新日期:2003-08-01 00:00:00
abstract::We report here the first local and global Ca(2+) measurements made from in situ terminal arterioles. The advantages of the method are that there is minimal disturbance to the vessels, which retain their relationship to the tissue they are supplying (rat ureter) and the small size of vessel that can be studied. Good lo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00019-8
更新日期:2003-07-01 00:00:00
abstract::P2Y(12) antagonists such as clopidogrel and AR-C69931MX inhibit aggregation by antagonizing the effects of ADP at P2Y(12) receptors on platelets. Agents such as PGE(1) also inhibit aggregation by stimulating adenylate cyclase to produce cAMP, which interferes with Ca(2+) mobilization within the cell. Since one facet o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00170-2
更新日期:2004-01-01 00:00:00
abstract::A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90057-8
更新日期:1995-11-01 00:00:00
abstract::Calcium-activated potassium channels are important in a variety of smooth muscles, contributing to excitability and contractility. In the myometrium previous work has focussed on the large conductance channels (BK), and the role of small conductance channels (SK) has received scant attention, despite the finding that ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.11.004
更新日期:2010-01-01 00:00:00
abstract::The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) mediates increases in the intracellular concentration of Ca(2+) ([Ca(2+)]i) during fertilization in mammalian eggs. The activity of IP3R1 is enhanced during oocyte maturation, and phosphorylations by M-phase kinases are thought to positively regulate the activit...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.07.004
更新日期:2015-11-01 00:00:00
abstract::The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(82)90004-5
更新日期:1982-08-01 00:00:00
abstract::The effects of the unsaturated fatty acids, arachidonic and oleic acid, on the influx of Ca2+ activated by depletion of intracellular stores with thapsigargin were investigated in various cell types. By using a Ca2+ free/Ca2+ reintroduction protocol, we observed that arachidonic acid (2 to 5 microM) inhibited thapsiga...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90031-2
更新日期:1997-05-01 00:00:00
abstract::Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Cytoplasmic calcium concentration was measured in primary cultured cells loaded with Fura-2. ET-1 (100 nM) caused c...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00085-x
更新日期:2003-09-01 00:00:00
abstract::An enzymatically active Ca2+-stimulated ATPase has been isolated from the sarcolemmal sheets of human smooth muscle (myometrium). Ca2+-ATPase activity was quantitated in an assay medium which simulated the characteristic free ionic concentrations of the cytosol. New computer programs for calculating the composition of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90001-5
更新日期:1983-10-01 00:00:00
abstract::Cellular Ca2+ signals are often constrained to cytosolic micro- or nano-domains where stochastic openings of Ca2+ channels cause large fluctuations in local Ca2+ concentration (Ca2+ 'noise'). With the advent of TIRF microscopy to image the fluorescence of Ca2+-sensitive probes from attoliter volumes it has become poss...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102152
更新日期:2020-03-01 00:00:00
abstract::Reference treatment of advanced prostate cancer (PCa) relies on pharmacological or surgical androgen deprivation therapy. However, it is only temporarily efficient as tumor cells inevitably adapt to the low testosterone environment and become hormone-refractory (HRPCa). We observed that androgen removal in HRPCa-deriv...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.02.004
更新日期:2015-04-01 00:00:00
abstract::The ability of the ectodermal cells to be induced and to differentiate toward neural tissue, called neural competence, is acquired shortly before gastrulation and lost during late gastrula stages in Pleurodeles waltl embryos. We have examined ectodermal cells' neural competence in relation to the evolution of the dens...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90036-5
更新日期:1995-03-01 00:00:00
abstract::Here, we report a novel role for the cysteine protease calpain in store-operated calcium entry. Several structurally and mechanistically unrelated inhibitors of calpain inhibited Ca2+ entry activated in human platelets by thapsigargin-evoked Ca2+ store depletion or the physiological agonist thrombin, whereas inhibitor...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.05.008
更新日期:2007-02-01 00:00:00
abstract::The glucoregulatory hormones insulin and glucagon are released from the β- and α-cells of the pancreatic islets. In both cell types, secretion is secondary to firing of action potentials, Ca(2+)-influx via voltage-gated Ca(2+)-channels, elevation of [Ca(2+)](i) and initiation of Ca(2+)-dependent exocytosis. Here we di...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.11.006
更新日期:2012-03-01 00:00:00
abstract::Here we examine the potential coupling between the synthesis of secretory proteins and the sensitivity of exocytosis to the concentration of free Ca(2+) in the cytosol ([Ca(2+)](i)) in plant cell. We therefore monitor in tobacco protoplasts the excursion of the membrane capacitance in response to an elevation of [Ca(2...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.05.011
更新日期:2012-09-01 00:00:00
abstract::Muscarinic acetylcholine (ACh) receptors activate the phospholipase C signal transduction pathway to promote the formation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] and the consequent elevation of cytoplasmic calcium (Ca2+). The inositol phosphate and Ca(2+)-mobilization responses to ACh were analyzed in Xenopus ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90090-s
更新日期:1993-10-01 00:00:00
abstract::A preparation of sea urchin eggs permeabilized with digitonin (40 microM for 2.5 min) was used to study the kinetic characteristics of the two cellular compartments suspected to play a key role in cellular calcium transfer during fertilization: an ATP-dependent Ca2+ pool (Km = 0.47 microM; Vm = 0.48 nmol/min.mg protei...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(91)90003-w
更新日期:1991-04-01 00:00:00
abstract::A characteristic feature of developing neural networks is spontaneous periodic activity. In the developing retina, retinal ganglion cells fire bursts of action potentials that drive large increases in intracellular calcium concentration with a periodicity of minutes. These periodic bursts of action potentials propagat...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.01.010
更新日期:2005-05-01 00:00:00
abstract::The oscillations in cytosolic Ca2+ evoked in pancreatic exocrine acinar cells by submaximal concentrations of the two phosphoinositidase-coupled agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8) have very different temporal patterns. In the present study we use digital video imaging of Fura-2 fluore...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90096-o
更新日期:1993-11-01 00:00:00
abstract::NAADP has been shown to act as a second messenger in a wide range of systems from plants to mammalian cells. Although it had always been considered as a canonical second messenger, recent work has shown that it is also active when applied extracellularly. It has also been suggested that NAADP might have a direct actio...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.10.002
更新日期:2007-06-01 00:00:00
abstract::In the complex signalling interplay that allows extracellular signals to be decoded into activation of apoptotic cell death, Ca(2+) plays a significant role. This is supported not only by evidence linking alterations in Ca(2+) homeostasis to the triggering of apoptotic (and in some cases necrotic) cell death, but also...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143416002002014
更新日期:2002-11-01 00:00:00
abstract::Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.01.018
更新日期:2005-05-01 00:00:00
abstract::Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90074-4
更新日期:1998-01-01 00:00:00
abstract::N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90004-e
更新日期:1990-04-01 00:00:00