Novel pathway for an old neurotransmitter: dopamine-induced neuronal calcium signalling via receptor-independent mechanisms.

abstract：:We describe the construction of a high-resolution confocal laser-scanning microscope, and illustrate its use for studying elementary Ca2+ signalling events in cells. An avalanche photodiode module and simple optical path provide a high efficiency system for detection of fluorescence signals, allowing use of a small co...

journal_title：Cell calcium

authors： Parker I,Callamaras N,Wier WG

更新日期：1997-06-01 00:00:00

abstract：:Changes in cytosolic free Ca(2+) concentration ([Ca(2+)]i) were monitored optically in hair cells mechanically isolated from frog semicircular canals using the membrane-impermeant form of the Ca(2+)-selective dye Oregon Green 488 BAPTA-1 (OG, 100 microM). Cells stimulated by depolarization under whole-cell voltage cla...

journal_title：Cell calcium

authors： Rispoli G,Martini M,Rossi ML,Mammano F

更新日期：2001-08-01 00:00:00

abstract：:NMDA receptors (NMDARs) are glutamate-gated ion channels involved in excitatory synaptic transmission and in others physiological processes such as synaptic plasticity and development. The overload of Ca2+ ions through NMDARs, caused by an excessive activation of receptors, leads to excitotoxic neuronal cell death. Fo...

journal_title：Cell calcium

authors： Plutino S,Sciaccaluga M,Fucile S

更新日期：2019-06-01 00:00:00

abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...

journal_title：Cell calcium

authors： Barclay JW,Morgan A,Burgoyne RD

更新日期：2005-09-01 00:00:00

abstract：:The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

journal_title：Cell calcium

authors： Tiffert T,Lew VL

更新日期：2001-11-01 00:00:00

abstract：:The effects of acute (3 h) and chronic (30 h) in vivo infusions of Escherichia coli endotoxin on the Ca2+ homeostasis of rat spleen cells was investigated. Conditions were established for obtaining reliable estimates of [Ca2+]i in these cells using the newly-developed Ca2+ indicator Fluo-3. The resting [Ca2+]i of sple...

journal_title：Cell calcium

authors： Hagar AF,Spitzer JA

更新日期：1992-02-01 00:00:00

abstract：:Voltage activated L-type Ca(2+) channels are the principal Ca(2+) channels in intestinal smooth muscle cells. They comprise the ion conducting Ca(V)1 pore and the ancillary subunits alpha(2)delta and beta. Of the four Ca(V)beta subunits Ca(V)beta(3) is assumed to be the relevant Ca(V)beta protein in smooth muscle. In ...

journal_title：Cell calcium

authors： Held B,Tsvilovskyy V,Meissner M,Kaestner L,Ludwig A,Mossmang S,Lipp P,Freichel M,Flockerzi V

更新日期：2007-10-01 00:00:00

abstract：:The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by Ca...

journal_title：Cell calcium

authors： Xu L,Gomez AC,Pasek DA,Meissner G,Yamaguchi N

更新日期：2017-09-01 00:00:00

abstract：:Fluorescent calcium indicators have been widely used to assess cytoplasmic calcium concentration in cells. To examine the role of calcium ions on different physiological functions (e.g. in case of liver; bile secretion, glucose metabolism, etc.) there is a need for whole organ studies. We have developed a technique to...

journal_title：Cell calcium

authors： Ruttner Z,Ligeti L,Reinlib L,Hines K,McLaughlin AC

更新日期：1993-06-01 00:00:00

abstract：:Studies of Ca2+ transport pathways in exocrine gland cells have been useful, chiefly because of the polarized nature of the secretory epithelial cells. In pancreatic acinar cells, for example, Ca2+ reloading of empty intracellular stores can occur solely via Ca2+ entry through the basal part of the plasma membrane. On...

journal_title：Cell calcium

authors： Petersen OH

更新日期：2003-05-01 00:00:00

abstract：:Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...

journal_title：Cell calcium

authors： Oiki S,Yamamoto T,Okada Y

更新日期：1994-03-01 00:00:00

abstract：:The surface dynamics of neurotransmitter receptors and transporters, as well as ion channels, has been well-documented in neurons, revealing complex molecular behaviour and key physiological functions. However, our understanding of the membrane trafficking and dynamics of the signalling molecules located at the plasma...

journal_title：Cell calcium

authors： Ciappelloni S,Murphy-Royal C,Dupuis JP,Oliet SHR,Groc L

更新日期：2017-11-01 00:00:00

abstract：:In addition to its role in artery contraction, Rho kinase (ROCK) is reported to be involved in the Ca(2+) response to vasoconstrictor agonist in rat aorta. However the signaling pathway mediated by ROCK had not been investigated so far and it was not known whether ROCK also contributed to Ca(2+) signaling in cultured ...

journal_title：Cell calcium

authors： Martinsen A,Baeyens N,Yerna X,Morel N

更新日期：2012-12-01 00:00:00

abstract：:The oscillations in cytosolic Ca2+ evoked in pancreatic exocrine acinar cells by submaximal concentrations of the two phosphoinositidase-coupled agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8) have very different temporal patterns. In the present study we use digital video imaging of Fura-2 fluore...

journal_title：Cell calcium

authors： Lawrie AM,Toescu EC,Gallacher DV

更新日期：1993-11-01 00:00:00

abstract：:Antisense oligodeoxynucleotides (AS-ODNs) were used in combination with transient functional expression of the cardiac Na(+)-Ca2+ exchanger (NCX1) to correlate suppression of the Na(+)-Ca2+ exchange function with down-regulation of NCX1 protein expression. In a de-novo expression system (Sf9 cells), a decrease in both...

journal_title：Cell calcium

authors： Egger M,Porzig H,Niggli E,Schwaller B

更新日期：2005-03-01 00:00:00

abstract：:Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca2+ mobilizing second messenger which triggers Ca2+ release in both sea urchin egg homogenates and in mammalian cells. The NAADP binding protein has not been identified and the regulation of NAADP mediated Ca2+ release remains controversial. To address...

journal_title：Cell calcium

authors： Asfaha TY,Gunaratne GS,Johns ME,Marchant JS,Walseth TF,Slama JT

更新日期：2019-11-01 00:00:00

abstract：:Agonist-induced calcium (Ca) mobilization is accompanied by Ca efflux, presumably reflecting the rise in Ca concentration at the cytosolic surface of the cell membrane. We studied the relationship between Ca efflux and intracellular Ca mobilization in Xenopus oocytes. Elevation of cytosolic Ca by a direct injection of...

journal_title：Cell calcium

authors： Shapira H,Lupu-Meiri M,Lipinsky D,Oron Y

更新日期：1996-03-01 00:00:00

abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...

journal_title：Cell calcium

authors： Wuytack F,Raeymaekers L,Missiaen L

更新日期：2002-11-01 00:00:00

abstract：:Depletion of calcium in the extracellular medium used to incubate first trimester human placental minces resulted in a significant decrease in the quantity of immunoreactive hCG in the medium and a corresponding increase in the tissue. In contrast, when secretion of newly synthesised hCG was monitored in the absence o...

journal_title：Cell calcium

authors： Sharma SC,Rao AJ

更新日期：1993-09-01 00:00:00

abstract：:The regulation of the Ca2+ -induced Ca2+ release (CICR) from intracellular stores is a critical step in the cardiac cycle. The inherent positive feedback of CICR should make it a self-regenerating process. It is accepted that CICR must be governed by some negative control, but its nature is still debated. We explore h...

journal_title：Cell calcium

authors： Zoghbi ME,Copello JA,Villalba-Galea CA,Vélez P,Diaz Sylvester PL,Bolaños P,Marcano A,Fill M,Escobar AL

更新日期：2004-08-01 00:00:00

abstract：:The highly selective fluorescent Ca2+ indicator 'quin 2' has been loaded into ram and boar spermatozoa as the acetoxymethyl ester, 'quin 2/AM', which is hydrolysed and trapped in the cytoplasm. Loadings of several mM were not toxic to spermatozoa as judged by motility. Fluorescence measurements (mean +/- S.E.M.) indic...

journal_title：Cell calcium

authors： Simpson AM,White IG

更新日期：1988-02-01 00:00:00

abstract：:Katz and co-workers showed that Ca(2+) triggers exocytosis. The existence of sub-micrometer domains of greater than 100 microM [Ca(2+)](i) was postulated on theoretical grounds. Using a modified, low-affinity aequorin, Llinas et al. were the first to demonstrate the existence of Ca(2+) 'microdomains' in squid presynap...

journal_title：Cell calcium

authors： Oheim M,Kirchhoff F,Stühmer W

更新日期：2006-11-01 00:00:00

abstract：:Corticosteroid hormones, which are released in high amounts after stress, easily pass the blood-brain-barrier. In the brain they bind to intracellular receptors which act as transcriptional regulators. These receptors are highly expressed in neurons of the hippocampal formation and the amygdala, areas that play a role...

journal_title：Cell calcium

authors： Joëls M,Karst H

更新日期：2012-03-01 00:00:00

abstract：:Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...

journal_title：Cell calcium

authors： Razani-Boroujerdi S,Partridge LD,Sopori ML

更新日期：1994-12-01 00:00:00

abstract：:Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...

journal_title：Cell calcium

authors： Astashkin EI,Surin AM,Mikhna MG,Nikolaeva IS,Lazarev AV,Gukovskaya AS

更新日期：1990-06-01 00:00:00

abstract：:The high capacity, low affinity Ca(2+) storage protein chromogranins are marker proteins of secretory granules that contain the most Ca(2+) in secretory cells. Along with the abundantly expressed chromogranins, the IP(3)R/Ca(2+) channels, the major intracellular Ca(2+) channels, are also expressed in secretory granule...

journal_title：Cell calcium

authors： Yoo SH,Hur YS

更新日期：2012-03-01 00:00:00

abstract：:Intracellular calcium signals activated by growth factors in endothelial cells during angiogenesis regulate cytosolic and nuclear events involved in survival, proliferation and motility. Among the intracellular messengers released after proangiogenic stimulation (bFGF, VEGF), arachidonic acid (AA), nitric oxide (NO) a...

journal_title：Cell calcium

authors： Tomatis C,Fiorio Pla A,Munaron L

更新日期：2007-03-01 00:00:00

abstract：:Previously we demonstrated that stimulation of cultured neonatal rat ventricular myocytes by either alpha 1-adrenergic agonist or endothelin-1 resulted in a rapid formation of total inositolphosphates, although the levels of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate did not rise significantly...

journal_title：Cell calcium

authors： De Jonge HW,Atsma DE,van der Valk-Kokshoorn EJ,van Heugten HA,van der Laarse A,Lamers JM

更新日期：1995-12-01 00:00:00

abstract：:Smooth muscle contraction is regulated by changes in cytosolic Ca(2+) concentration ([Ca(2+)](i)). In response to stimulation, Ca(2+) increase in a single cell can propagate to neighbouring cells through gap junctions, as intercellular Ca(2+) waves. To investigate the mechanisms underlying Ca(2+) wave propagation betw...

journal_title：Cell calcium

authors： Halidi N,Boittin FX,Bény JL,Meister JJ

更新日期：2011-11-01 00:00:00

abstract：:Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...

journal_title：Cell calcium

authors： Zimmermann B

更新日期：1998-01-01 00:00:00