Kinetics of inhibition of the plasma membrane calcium pump by vanadate in intact human red cells.

abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...

journal_title：Cell calcium

authors： Barclay JW,Morgan A,Burgoyne RD

更新日期：2005-09-01 00:00:00

abstract：:A characteristic feature of developing neural networks is spontaneous periodic activity. In the developing retina, retinal ganglion cells fire bursts of action potentials that drive large increases in intracellular calcium concentration with a periodicity of minutes. These periodic bursts of action potentials propagat...

journal_title：Cell calcium

authors： Firth SI,Wang CT,Feller MB

更新日期：2005-05-01 00:00:00

abstract：:A whole range of cell functions are regulated by the free cytosolic Ca(2+)concentration. Activator Ca(2+)from the extracellular space enters the cell through various types of Ca(2+)channels and sometimes the Na(+)/Ca(2+)-exchanger, and is actively extruded from the cell by Ca(2+)pumps and Na(+)/Ca(2+)-exchangers. Acti...

journal_title：Cell calcium

authors： Missiaen L,Robberecht W,van den Bosch L,Callewaert G,Parys JB,Wuytack F,Raeymaekers L,Nilius B,Eggermont J,De Smedt H

更新日期：2000-07-01 00:00:00

abstract：:Here we examine the potential coupling between the synthesis of secretory proteins and the sensitivity of exocytosis to the concentration of free Ca(2+) in the cytosol ([Ca(2+)](i)) in plant cell. We therefore monitor in tobacco protoplasts the excursion of the membrane capacitance in response to an elevation of [Ca(2...

journal_title：Cell calcium

authors： Sutter JU,Denecke J,Thiel G

更新日期：2012-09-01 00:00:00

abstract：:Calcium regulates the proliferation and differentiation of keratinocytes both in vivo and in vitro. Elevated extracellular Ca2+ concentration ([Ca2+]o) raises the intracellular free calcium ([Ca2+]i) and activates differentiation-related genes. Cells lacking the calcium-sensing receptor (CaR) fail to respond to [Ca2+]...

journal_title：Cell calcium

authors： Tu CL,Oda Y,Komuves L,Bikle DD

更新日期：2004-03-01 00:00:00

abstract：:The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...

journal_title：Cell calcium

authors： Prosser BL,Hernández-Ochoa EO,Schneider MF

更新日期：2011-10-01 00:00:00

abstract：:Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...

journal_title：Cell calcium

authors： Fliegert R,Hölzer HT,Guse AH

更新日期：2018-12-01 00:00:00

abstract：:Quartered pituitaries from castrated (72 h) +/- estradiol (E2)-treated (24 h) rats were used in a perifusion system to investigate estradiol modulation of ionomycin and ionomycin + PMA stimulated LH secretion. Estradiol enhanced the LH responses to GnRH (1 nM) and ionomycin (10 microM), and was necessary for the manif...

journal_title：Cell calcium

authors： Fahmy NW,Bourne GA

更新日期：1993-01-01 00:00:00

abstract：:Calbindin D28K (formerly known as vitamin D-dependent calcium binding protein and referred to here as calbindin) is found in a wide variety of tissues, but only in certain cells within those tissues. Apart from its ability to bind calcium, nothing is known about its function in these cells. To investigate its role we ...

journal_title：Cell calcium

authors： Muir EM,Lawson DE,Sepúlveda FV,O'Brien JA,Harding M

更新日期：1993-04-01 00:00:00

abstract：:Pancreatic islets produce pulses of insulin and other hormones that maintain normal glucose homeostasis. These micro-organs possess exquisite glucose-sensing capabilities, allowing for precise changes in pulsatile insulin secretion in response to small changes in glucose. When communication among these cells is disrup...

journal_title：Cell calcium

authors： Scarl RT,Corbin KL,Vann NW,Smith HM,Satin LS,Sherman A,Nunemaker CS

更新日期：2019-11-01 00:00:00

abstract：:To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 dete...

journal_title：Cell calcium

authors： Uto A,Arai H,Ogawa Y

更新日期：1991-01-01 00:00:00

abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...

journal_title：Cell calcium

authors： Famulski KS,Pikula S,Wrzosek A,Wojtczak AB

更新日期：1990-04-01 00:00:00

abstract：:Mathematical models are often used to elucidate mechanisms behind cytosolic Ca2+ oscillations. We have evaluated the use of mathematical modelling to analyse and quantify Ca2+ signal patterns, in single, adherent human neutrophils (PMN) after stimulation by the bacterial peptide N-formyl-methionyl-leucyl-phenylalanine...

journal_title：Cell calcium

authors： Røtnes JS,Røttingen JA

更新日期：1994-06-01 00:00:00

abstract：:We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown ...

journal_title：Cell calcium

authors： Martin VV,Beierlein M,Morgan JL,Rothe A,Gee KR

更新日期：2004-12-01 00:00:00

abstract：:Ca2+ efflux from dog red blood cells loaded with Ca2+ using the A23187 ionophore could be separated into two main components: (1) Mg- and ATP-dependent (active transport) and (2) dependent on external Na (K1/2 around 15 mM); at 80 microM internal free Ca the relative magnitudes of these fluxes were 70% and 30% respect...

journal_title：Cell calcium

authors： Altamirano AA,Beaugé L

更新日期：1985-12-01 00:00:00

abstract：:It has been reported that leukotriene B4 can translocate calcium across model membranes (Serhan et. al., (1982) J. Biol. Chem., 257: 4746). Such ionophoretic behavior could account for its biological effects. We have examined the effect of chromatographically pure leukotriene B4 on Ca2+ permeability when added exogeno...

journal_title：Cell calcium

authors： Holmes RP,Yoss NL,Marshall LA

更新日期：1987-12-01 00:00:00

abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...

journal_title：Cell calcium

authors： Wuytack F,Raeymaekers L,Missiaen L

更新日期：2002-11-01 00:00:00

abstract：:When Xenopus oocytes injected with rat brain poly(A)+RNA were voltage-clamped in a recording solution containing Ca2+, a depolarization pulse induced a transient current, ICl(Ca), which reflects calmodulin-mediated opening of endogenous Cl- channels in response to a Ca2+ influx through Ca2+ channels of brain origin. I...

journal_title：Cell calcium

authors： Kaneko S,Doi E,Watanabe H,Nomura Y

更新日期：1990-04-01 00:00:00

abstract：:Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...

journal_title：Cell calcium

authors： Honda Y,Miyazaki S

更新日期：1996-04-01 00:00:00

abstract：:Intercellular Ca(2+)-signaling, after mechanical stimulation of calf pulmonary artery endothelial cells (CPAE), was investigated with fluorescence video imaging. Mechanical stimulation evoked an intracellular Ca(2+)-response in the mechanically stimulated (MS) cell, proceeding to the neighboring (NB) cells as a Ca(2+)...

journal_title：Cell calcium

authors： Moerenhout M,Himpens B,Vereecke J

更新日期：2001-02-01 00:00:00

abstract：:Central to controlling intracellular calcium concentration ([Ca(2+)](i)) are a number of Ca(2+) transporters and channels with the L-type Ca(2+) channel, Na(+)-Ca(2+) exchanger and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) being of particular note in the heart. This review concentrates on the regulation of [Ca(2+)]...

journal_title：Cell calcium

authors： Dibb KM,Graham HK,Venetucci LA,Eisner DA,Trafford AW

更新日期：2007-10-01 00:00:00

abstract：:NAADP (nicotinic acid adenine dinucleotide phosphate) is a recently discovered second messenger, and as such, we have much yet to learn about its functions in health and disease. A bottleneck in this basic research is due to NAADP, like all second messengers, being charged to prevent it from leaking out of cells. This...

journal_title：Cell calcium

authors： Parkesh R,Lewis AM,Aley PK,Arredouani A,Rossi S,Tavares R,Vasudevan SR,Rosen D,Galione A,Dowden J,Churchill GC

更新日期：2008-06-01 00:00:00

abstract：:Calcium release-activated calcium (CRAC) channels have been the target of drug discovery for many years. The identification of STIM and Orai proteins as key components of CRAC channels greatly facilitated this process because their co-expression in cell lines produced electrophysiological currents (ICRAC) much larger ...

journal_title：Cell calcium

authors： Stauderman KA

更新日期：2018-09-01 00:00:00

abstract：:Palmitoylation (S-acylation) is the reversible conjugation of a fatty acid (usually C16 palmitate) to intracellular cysteine residues of proteins via a thioester linkage. Palmitoylation anchors intracellular regions of proteins to membranes because the palmitoylated cysteine is recruited to the lipid bilayer. NCX1 is ...

journal_title：Cell calcium

authors： Gök C,Fuller W

更新日期：2020-03-01 00:00:00

abstract：:The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...

journal_title：Cell calcium

authors： Viana F,Van den Bosch L,Missiaen L,Vandenberghe W,Droogmans G,Nilius B,Robberecht W

更新日期：1997-10-01 00:00:00

abstract：:To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as ...

journal_title：Cell calcium

authors： Hertelendy ZI,Patel DG,Knittel JJ

更新日期：1996-02-01 00:00:00

abstract：:Antigen-mediated mast cell (MC) degranulation is the critical early event in the induction of allergic reactions. Transient receptor potential channels (TRPC), particularly TRPC1, are thought to contribute to such MC activation. To explore the contribution of TRPC1 in MC-driven allergic reactions, we examined antigen-...

journal_title：Cell calcium

authors： Medic N,Desai A,Olivera A,Abramowitz J,Birnbaumer L,Beaven MA,Gilfillan AM,Metcalfe DD

更新日期：2013-05-01 00:00:00

abstract：:As pivotal players in cellular metabolism, mitochondria have a double-faceted role in the final decision of cell fate. This is true for all cell types, but it is even more important and intriguing in the cancer setting. Mitochondria regulate cell fate in many diverse ways: through metabolism, by producing ATP and othe...

journal_title：Cell calcium

authors： Genovese I,Vezzani B,Danese A,Modesti L,Vitto VAM,Corazzi V,Pelucchi S,Pinton P,Giorgi C

更新日期：2020-12-01 00:00:00

abstract：:Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...

journal_title：Cell calcium

authors： Razani-Boroujerdi S,Partridge LD,Sopori ML

更新日期：1994-12-01 00:00:00

abstract：:Hepatocytes stimulated with calcium-mobilising agonists generate free Ca transients whose frequency is modulated by hormone concentration. Importantly, the time-course of individual free Ca transients is independent of agonist dose but does change with agonist species. A receptor-controlled model in which protein kina...

journal_title：Cell calcium

authors： Cobbold PH,Sanchez-Bueno A,Dixon CJ

更新日期：1991-02-01 00:00:00