Abstract:
:The oscillations in cytosolic Ca2+ evoked in pancreatic exocrine acinar cells by submaximal concentrations of the two phosphoinositidase-coupled agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8) have very different temporal patterns. In the present study we use digital video imaging of Fura-2 fluorescence to map the spatial distribution of Ca2+ during the oscillating responses to these two agonists. The spatial patterns induced are very different for each of these agonists. ACh oscillations are sinusoidal and initiated at the secretory pole of these morphologically and functionally polarized cells. As they spread across the cell, pronounced gradients in Ca2+ develop that persist throughout the oscillating response. CCK-8 induces a series of discrete Ca2+ transients of longer duration and lower frequency. These elevations in Ca2+ arise slowly, throughout the cells and without any detectable gradients in Ca2+. We consider that the different spatiotemporal patterns can be explained on the basis of a physiologically relevant interaction between Ins(1,4,5)P3 and protein kinase C in second messenger-mediated Ca2+ signalling.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Lawrie AM,Toescu EC,Gallacher DVdoi
10.1016/0143-4160(93)90096-osubject
Has Abstractpub_date
1993-11-01 00:00:00pages
698-710issue
10eissn
0143-4160issn
1532-1991pii
0143-4160(93)90096-Ojournal_volume
14pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::The mechanism by which the depletion of intracellular Ca2+ stores stimulates Ca2+ influx is poorly understood. However, the coupling of depletion to influx is broken during mitosis [Preston, S.F. et. al., (1991) Cell Regul., 2, 915-925]. Thus, in interphase HeLa cells, activation of the histamine H1 receptor, or incub...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90042-5
更新日期:1993-05-01 00:00:00
abstract::In previous studies we have characterised various properties of capacitative Ca2+ entry (CCE) in different epithelia. After Ca2+ store depletion with PLC/InsP3-coupled agonists or by inhibition of store Ca2+ uptake, with for example thapsigargin, Ca2+ influx is activated. This leads to a sustained cellular response (e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90112-3
更新日期:1997-08-01 00:00:00
abstract::Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00143-x
更新日期:2003-10-01 00:00:00
abstract::Intracytoplasmic sperm injection (ICSI) into mammalian eggs induces repetitive rises in intracellular Ca2+ concentration ([Ca2+]i) which are the pivotal signal in fertilization. Spatiotemporal aspects of [Ca2+]i rises following ICSI into the periphery of mouse eggs were investigated with high-speed confocal microscopy...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.1999.0053
更新日期:1999-07-01 00:00:00
abstract::Synaptic transmission is a key signaling event, whereby an action potential-induced release of chemical neurotransmitters again generates a positive or negative electrical activity via opening of postsynaptic channels. Thereafter, information spreads through space, from the postsynaptic membranes to the dendrites, to ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(98)90113-0
更新日期:1998-02-01 00:00:00
abstract::Sarcoplasmic reticulum (SR) is abundant in uterine smooth muscle cells. The functional role of this organelle in the regulation of uterine myocytes is not fully understood. The data available in the literature suggest that SR plays a dual role: as a source of calcium and as a calcium sink shaping calcium transients pr...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2004.01.006
更新日期:2004-06-01 00:00:00
abstract::The Solute Carrier Family 24 (SLC24) belongs to the CaCA super family of Ca(2+)/cation antiporters and codes for five different K(+)- dependent Na(+)- Ca(2+) exchangers (NCKX1-5). NCKX proteins play a critical role in Ca(2+) homeostasis in a wide variety of biological processes such as vision, olfaction, enamel format...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.11.001
更新日期:2016-01-01 00:00:00
abstract::Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90070-6
更新日期:1996-04-01 00:00:00
abstract::In the cochlea, cell damage triggers intercellular Ca2+ waves that propagate through the glial-like supporting cells that surround receptor hair cells. These Ca2+ waves are thought to convey information about sensory hair cell-damage to the surrounding supporting cells within the cochlear epithelium. Mitochondria are ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.06.005
更新日期:2009-08-01 00:00:00
abstract::Calcium and calcium-dependent systems have been long implicated in the induction of epilepsy. We have previously observed that intracellular calcium ([Ca2+]i) levels remain elevated in cells undergoing epileptogenesis in the hippocampal neuronal culture (HNC) model. In this study, we employed the hippocampal neuronal ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2000.0146
更新日期:2000-09-01 00:00:00
abstract::Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.1999.0044
更新日期:1999-06-01 00:00:00
abstract::Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90060-4
更新日期:1994-03-01 00:00:00
abstract::The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.06.001
更新日期:2011-10-01 00:00:00
abstract::A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.06.012
更新日期:2005-09-01 00:00:00
abstract::The formation of a heterotetrameric complex between annexin 2 (anx 2) and S100A10 plays an important role in regulating the cellular distribution and biochemical properties of anx 2. A major distinction between the anx 2-S100A10 complex and other annexin-S100 complexes is that S100A10 binding to anx 2 occurs independe...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.11.001
更新日期:2008-08-01 00:00:00
abstract::Localized subcellular changes in Ca(2+) serve as important cellular signaling elements, regulating processes as diverse as neuronal excitability and gene expression. Studies of cellular Ca(2+) signaling have been greatly facilitated by the availability of fluorescent Ca(2+) indicators. The respective merits of differe...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.10.003
更新日期:2015-12-01 00:00:00
abstract::Polycystin-2, a member of the TRP family of calcium channels, is encoded by the human PKD2 gene. Mutations in that gene can lead to swelling of nephrons into the fluid-filled cysts of polycystic kidney disease. In addition to expression in tubular epithelial cells, human polycystin-2 is found in muscle and neuronal ce...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.03.003
更新日期:2005-06-01 00:00:00
abstract::A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(95)90027-6
更新日期:1995-10-01 00:00:00
abstract::It is well established that intracellular calcium ([Ca2+]i) controls the inotropic state of the myocardium, and evidence mounts that a "Ca2+ clock" controls the chronotropic state of the heart. Recent findings describe a calcium-activated nonselective cation channel (NSCCa) in various cardiac preparations sharing hall...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.06.003
更新日期:2013-09-01 00:00:00
abstract::The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.12.001
更新日期:2009-04-01 00:00:00
abstract::Transient receptor potential cation channel, subtype melastatin 2 (TRPM2), is important for several physiological functions, such as immune response or temperature regulation. Recently, the structure of full-length TRPM2 from zebrafish was published (Huang et al., 2018) proposing a new activation mechanism - is it rea...
journal_title:Cell calcium
pub_type: 评论,杂志文章
doi:10.1016/j.ceca.2018.11.001
更新日期:2018-12-01 00:00:00
abstract::Recent developments in microscopy and fluorescent indicators now make it possible to monitor the activity and localization of membrane ion channels by imaging Ca(2+) flux through individual channels. Such optical approaches have advantages over electrophysiological single-channel techniques in that they are less invas...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.10.008
更新日期:2005-04-01 00:00:00
abstract::NMDA receptors (NMDARs) are glutamate-gated ion channels involved in excitatory synaptic transmission and in others physiological processes such as synaptic plasticity and development. The overload of Ca2+ ions through NMDARs, caused by an excessive activation of receptors, leads to excitotoxic neuronal cell death. Fo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.04.001
更新日期:2019-06-01 00:00:00
abstract::Cellular senescence is a stable cell proliferation arrest induced by a variety of stresses including telomere shortening, oncogene activation and oxidative stress. This process plays a crucial role in many physiopathological contexts, especially during aging when cellular senescence favors development of age-related d...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2017.04.001
更新日期:2018-03-01 00:00:00
abstract::Calcium-permeable channels have traditionally been thought of as therapeutic targets in excitable cells. For instance, voltage-operated Ca2+ channels in neurones and smooth muscle cells for neurological and cardiovascular diseases although calcium-permeable channels are also functionally important in electrically non-...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00060-5
更新日期:2003-05-01 00:00:00
abstract::The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washe...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90002-9
更新日期:1987-08-01 00:00:00
abstract::TRPA1, a Ca(2+)-permeable cation channel that is expressed in sensory neurones, is involved in the perception of chemical irritants and mechanical hyperalgesia. TRPA1 is activated by either covalent or reversible binding of various chemical compounds, including allylisothiocyanate or acrolein, and is further sensitise...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.08.001
更新日期:2009-02-01 00:00:00
abstract::Here, we report a novel role for the cysteine protease calpain in store-operated calcium entry. Several structurally and mechanistically unrelated inhibitors of calpain inhibited Ca2+ entry activated in human platelets by thapsigargin-evoked Ca2+ store depletion or the physiological agonist thrombin, whereas inhibitor...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.05.008
更新日期:2007-02-01 00:00:00
abstract::The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2001.0233
更新日期:2001-10-01 00:00:00
abstract::Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.05.008
更新日期:2013-09-01 00:00:00