Abstract：
:Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ transients by mobilization of Ca2+ from the endoplasmic reticulum. With 2 mM external Ca2+, the transients included both extra- and intracellular components. The recruitment of intracellular Ca2+ increased as the stimulus duration decreased. At the threshold of 200-300 nM, the transients were amplified by calcium-induced calcium release. We conclude that nanosecond stimuli mimic Ca2+ signaling while bypassing the usual receptor- and channels-mediated cascades. The recruitment of the intracellular Ca2+ can be controlled by the duration of the stimulus.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Semenov I,Xiao S,Pakhomova ON,Pakhomov AGdoi
10.1016/j.ceca.2013.05.008subject
Has Abstractpub_date
2013-09-01 00:00:00pages
145-50issue
3eissn
0143-4160issn
1532-1991pii
S0143-4160(13)00083-3journal_volume
54pub_type
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