Abstract：
:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel playing a major role in Ca2+ signaling. Three isoforms of IP3R have been identified and most cell types express different proportions of each isoform. The DT40 B lymphocyte cell line lacking all three IP3R isoforms (DT40IP3R-KO cells) represents an excellent model to re-express any recombinant IP3R and analyze its specific properties. In the study presented here, we confirmed that DT40IP3R-KO cells do not express any IP3-sensitive Ca2+ release channel. However, with an immunoblot approach and a [3H]IP3 binding approach we demonstrated the presence of a C-terminally truncated form of IP3R type III in the cytosolic fraction of DT40IP3R-KO cells. We further showed that this truncated IP3R retained the ability to couple to the Ca2+ entry channel TRPC6. Therefore, a word of caution is offered about the interpretation of results obtained in using DT40IP3R-KO cells to study the cellular mechanisms of Ca2+ entry.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Guillemette J,Caron AZ,Regimbald-Dumas Y,Arguin G,Mignery GA,Boulay G,Guillemette Gdoi
10.1016/j.ceca.2004.03.005keywords:
subject
Has Abstractpub_date
2005-02-01 00:00:00pages
97-104issue
2eissn
0143-4160issn
1532-1991pii
S0143416004000867journal_volume
37pub_type
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