Abstract:
:Extracellular nucleotides are potent Ca2+ mobilizing agents. A variety of receptors for extracellular ATP are recognised. Some are involved in fast neuronal transmission and operate as ligand-gated ion channels. Others are involved in the paracrine or autocrine modulation of cell function. Many receptors of this type are coupled to phosphoinositide-specific phospholipase C and, in some cases, other phospholipases. One of these receptors (P2z), however, also appears to operate, at least in part, as a ligand-gated ion channel. Pharmacological data suggest that one nucleotide receptor subtype (currently designated P2U) responds selectively to either a purine nucleotide, ATP, or a pyrimidine nucleotide, UTP. According to an alternative view, ATP and UTP recognise distinct receptors. Because of the diversity of receptors for extracellular nucleotides this may be the case in some cells. Nevertheless, a G-protein coupled receptor that confers both ATP and UTP sensitivity has been cloned, expressed in cultured cell lines and sequenced. This receptor appears to have two ligand binding domains that may partially overlap. The nature of this overlap is discussed and a simple model presented. Activation of the receptor protein via one or other ligand binding domain may underlie some of the more subtle differences between the effects of ATP and UTP.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Conigrave AD,Jiang Ldoi
10.1016/0143-4160(95)90080-2subject
Has Abstractpub_date
1995-02-01 00:00:00pages
111-9issue
2eissn
0143-4160issn
1532-1991pii
0143-4160(95)90080-2journal_volume
17pub_type
杂志文章,评审相关文献
CELL CALCIUM文献大全abstract::Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90074-4
更新日期:1998-01-01 00:00:00
abstract::R24571 a derivative of the antimycotic miconazole, appears to be 5 to 8 times more potent than trifluoperazine in its ability to inhibit the calmodulin-dependent phosphorylation of cardiac sarcoplasmic reticulum vesicles. The cAMP-dependent protein kinase mediated phosphorylation of cardiac sarcoplasmic reticulum was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90039-8
更新日期:1983-04-01 00:00:00
abstract::Ca2+ pumps are essential for removing cytosolic Ca2+ either across the plasma membrane (PM) or into internal organelles such as the sarcoplasmic reticulum (SR). Four genes (PMCA1, PMCA2, PMCA3 and PMCA4) have been reported to encode the PM Ca2+ pumps and three (SERCA1, SERCA2 and SERCA3) to encode the SR Ca2+ pumps. T...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(92)90025-n
更新日期:1992-01-01 00:00:00
abstract::A characteristic feature of developing neural networks is spontaneous periodic activity. In the developing retina, retinal ganglion cells fire bursts of action potentials that drive large increases in intracellular calcium concentration with a periodicity of minutes. These periodic bursts of action potentials propagat...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.01.010
更新日期:2005-05-01 00:00:00
abstract::Reference treatment of advanced prostate cancer (PCa) relies on pharmacological or surgical androgen deprivation therapy. However, it is only temporarily efficient as tumor cells inevitably adapt to the low testosterone environment and become hormone-refractory (HRPCa). We observed that androgen removal in HRPCa-deriv...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.02.004
更新日期:2015-04-01 00:00:00
abstract::Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90007-s
更新日期:1993-06-01 00:00:00
abstract::In HEK293 cells, transfected with the Ca2+ channel protein TRPV6, Ca2+ influx is increased and TRPV6 is tyrosine phosphorylated following addition of the tyrosine phosphatase inhibitor N,N-dimethyl-hydroxamido hydroxovanadate to cells. This effect of DMHV is enhanced by co-transfection of cells with the tyrosine kinas...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.11.008
更新日期:2007-07-01 00:00:00
abstract::We analyzed spatio-temporal characteristics of Ca2+ transients in the cytosol and the nucleus of cultured neonatal rat heart cells using confocal imaging with Indo-1 and Fluo-3. In resting heart muscle cells, nuclear [Ca2+] was maintained lower than the cytosolic level. The rise in nuclear [Ca2+], during either E-C co...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90031-4
更新日期:1995-03-01 00:00:00
abstract::Endothelin-1 (ET-1), a potent vasoactive peptide with a pathogenic role in vascular diseases, has been shown to induce the activation of ERK1/2, PKB and the expression of a transcriptional regulator, the early growth response 1 (Egr-1), key mediators of hypertrophic and proliferative responses in vascular smooth muscl...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.10.002
更新日期:2013-12-01 00:00:00
abstract::We previously found in single channel studies that ryanodine receptor (RyR) channel activity can be made insensitive to block by Mg2+ when terminal cisternae of sarcoplasmic reticulum, incorporated into planar bilayers, are treated with protein kinase A (PKA) or Ca2+/calmodulin dependent protein kinase type II (CamPK ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90064-0
更新日期:1995-09-01 00:00:00
abstract::Hard end is a physiological disorder of pear fruit that is frequently observed in the 'Whangkeumbae' (Pyrus pyrifolia) variety, however, the mechanisms that are involved in its development are poorly understood. In this study, we explored the causes of hard end disorder in pear fruit in relation to calcium deficiency....
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2017.11.002
更新日期:2018-05-01 00:00:00
abstract::We describe the construction of a high-resolution confocal laser-scanning microscope, and illustrate its use for studying elementary Ca2+ signalling events in cells. An avalanche photodiode module and simple optical path provide a high efficiency system for detection of fluorescence signals, allowing use of a small co...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90055-5
更新日期:1997-06-01 00:00:00
abstract::In many animals, a rise in intracellular calcium levels is the trigger for egg activation, the process by which an arrested mature oocyte transitions to prepare for embryogenesis. In nearly all animals studied to date, this calcium rise, and thus egg activation, is triggered by the fertilizing sperm. However in the in...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2012.11.008
更新日期:2013-01-01 00:00:00
abstract::Odontoblasts are involved in the transduction of stimuli applied to exposed dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 (TRPV1)-mediated signaling remain to be clarified. We investigated physiological ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.05.002
更新日期:2012-08-01 00:00:00
abstract::The Bcl-2 protein, best known for its ability to inhibit apoptosis, interacts with the inositol 1,4,5-trisphosphate receptor (IP(3)R) Ca(2+) channel to regulate IP(3)-mediated Ca(2+) release from the endoplasmic reticulum. This review summarizes the current state of knowledge regarding the interaction of Bcl-2, and al...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.05.011
更新日期:2011-09-01 00:00:00
abstract::Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90076-0
更新日期:1994-12-01 00:00:00
abstract::Antisense oligodeoxynucleotides (AS-ODNs) were used in combination with transient functional expression of the cardiac Na(+)-Ca2+ exchanger (NCX1) to correlate suppression of the Na(+)-Ca2+ exchange function with down-regulation of NCX1 protein expression. In a de-novo expression system (Sf9 cells), a decrease in both...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.10.006
更新日期:2005-03-01 00:00:00
abstract::Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the alpha-subunit of Gq (Ad-EF1-dnG alpha q) and the other expressing the wild-type alpha-subunit of Gq (Ad-EF1-wtG ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90077-x
更新日期:1998-08-01 00:00:00
abstract::Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90070-6
更新日期:1996-04-01 00:00:00
abstract::Factors contributing to "local control" of Ca2+ release in cardiac myocytes are incompletely understood. We induced local release of Ca2+ by regional exposure of mouse atrial and ventricular myocytes to 10mM caffeine for 500 ms using a rapid solution switcher. Propagation of Ca2+ release was imaged by means of a Nipko...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.03.004
更新日期:2005-07-01 00:00:00
abstract::Aluminium lactate provokes the same modification of 45Ca(2+)-uptake by Ehrlich ascites tumour cells as does ferric lactate. The increase of uptake is metal-complex concentration-dependent, and in contrast to ferric lactate, is only partially inhibited by albumin. Under the same experimental conditions, chromic lactate...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90062-w
更新日期:1992-05-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons. Mutations in Cu/Zn superoxide dismutase-1 (SOD1) cause familial ALS but the molecular mechanisms whereby these mutations induce motor neuron death remain controversial. Here, we show that stable overex...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.02.004
更新日期:2011-04-01 00:00:00
abstract::The store-operated, calcium release-activated calcium current I(CRAC) is activated by the depletion of inositol 1,4,5-trisphosphate (IP(3))-sensitive stores. The significantly different dose-response relationships of IP(3)-mediated Ca(2+) release and CRAC channel activation indicate that I(CRAC) is activated by a func...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.12.001
更新日期:2009-04-01 00:00:00
abstract::C3H/10T1/2 mouse embryo fibroblasts stimulated by a steady electric field (10 V/cm) for 30 min exhibited lamellar retraction on the sides facing the electrodes. Some cells elongated and preferentially oriented with their long axis perpendicular to the field direction. Depletion of external calcium or blockage of calci...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(85)90012-0
更新日期:1985-06-01 00:00:00
abstract::We have found that the changes in calcium metabolism in rabbit neutrophils produced by the chemotactic synthetic peptide f-Met-Leu-Phe are not sensitive to the calcium chelator EGTA. The present results demonstrate unambiguously that the previously described chemotactic factor induced changes in 45Ca fluxes in rabbit ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90049-0
更新日期:1983-02-01 00:00:00
abstract::Calcium-activated potassium channels are important in a variety of smooth muscles, contributing to excitability and contractility. In the myometrium previous work has focussed on the large conductance channels (BK), and the role of small conductance channels (SK) has received scant attention, despite the finding that ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.11.004
更新日期:2010-01-01 00:00:00
abstract::Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90054-x
更新日期:1990-06-01 00:00:00
abstract::In the cochlea, cell damage triggers intercellular Ca2+ waves that propagate through the glial-like supporting cells that surround receptor hair cells. These Ca2+ waves are thought to convey information about sensory hair cell-damage to the surrounding supporting cells within the cochlear epithelium. Mitochondria are ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.06.005
更新日期:2009-08-01 00:00:00
abstract::There is a close association of peripheral sarcoplasmic reticulum (SR), containing IP3 receptors, and regions of the plasma membrane enriched in the Na+/Ca2+ exchanger in smooth muscle. We have tested the possibility in rat uterine smooth muscle that Ca2+ released from the SR is preferentially removed from the cytosol...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90018-x
更新日期:1997-11-01 00:00:00
abstract::To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90081-0
更新日期:1996-02-01 00:00:00