Abstract：
:Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concentration ([Ca2+]i) during the process of distinct target cell killing. A rapid [Ca2+]i rise in NK was generated within 2 min after contact with any target. For induction of necrosis, the [Ca2+]i rise in NK reached a peak of 580 +/- 195 nM (mean +/- SD, n = 41) and was followed by an abrupt large [Ca2+]i rise in target cells probably due to Ca2+ entry through pores formed by perforin which was released from NK. The interval between onsets of NK and target Ca2+ responses (Tnk-t) was shorter than 3 min in most cases. Target cells were soon permeabilized, and NK Ca2+ responses ceased in 14 +/- 7 min. For induction of apoptosis, NK Ca2+ responses were relatively smaller (405 +/- 125 nM, n = 16), and Tnk-t was widely scattered up to 30 min. NK Ca2+ responses lasted much longer in a form of Ca2+ oscillations (total duration, 66 +/- 33 min) in parallel with target Ca2+ responses, and ceased as apoptotic changes such as cellular and nuclear fragmentation advanced. The difference in Tnk-t suggests that rapid synchronous release of perforin tends to cause necrosis rather than apoptosis. The duration of NK Ca2+ responses suggests that some 'off signals' may be transmitted to NK following recognition of target cell damage.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Honda Y,Miyazaki Sdoi
10.1016/s0143-4160(96)90070-6subject
Has Abstractpub_date
1996-04-01 00:00:00pages
297-306issue
4eissn
0143-4160issn
1532-1991pii
S0143-4160(96)90070-6journal_volume
19pub_type
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