Inositol 1,4,5-trisphosphate and ryanodine receptors mobilize calcium from a common functional pool in human U373 MG cells.


:This investigation concentrates on the change in Ca(2+) concentration ([Ca(2+)]) caused by ryanodine in U373 MG cells. This cell type from a human astrocytoma is a unique cellular model because it only expresses the type 3 ryanodine receptor (RyR3), which is generally the least abundant isoform. In the presence of physiological [Ca(2+)] in the extracellular medium, U373 MG cells are caffeine-insensitive, even after forskolin treatment, and ryanodine-sensitive only when an unusually high concentration (30 microM) is applied. Xestospongin C behaves like thapsigargin and therefore cannot be used as a selective antagonist of inositol 1,4,5-trisphosphate receptors (InsP(3)Rs). After ryanodine challenge, addition of an analog of Substance P (SP), which should deplete InsP(3)-sensitive stores, has no effect on [Ca(2+)](i). After thapsigargin treatment, which unmasks the calcium leak from intracellular stores, neither ryanodine nor SP change [Ca(2+)](i), suggesting that thapsigargin completely depletes the ryanodine-sensitive and the InsP(3)-sensitive stores of U373 MG cells. Finally, in experiments monitoring the [Ca(2+)] in intracellular stores, InsP(3) stimulation of permeabilized cells causes a decrease in [Ca(2+)] that is not affected by subsequent ryanodine treatment. Our results support the conclusion that U373 MG cells express both InsP(3)Rs and RyRs that can individually or in combination mobilize only one functional Ca(2+) pool.


Cell Calcium


Cell calcium


Galiano M,Gasparre G,Lippe C,Cassano G





Has Abstract


2004-11-01 00:00:00














  • Two EF-hand motifs in ryanodine receptor calcium release channels contribute to isoform-specific regulation by calmodulin.

    abstract::The mammalian ryanodine receptor Ca2+ release channel (RyR) has a single conserved high affinity calmodulin (CaM) binding domain. However, the skeletal muscle RyR1 is activated and cardiac muscle RyR2 is inhibited by CaM at submicromolar Ca2+. This suggests isoform-specific domains are involved in RyR regulation by Ca...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Xu L,Gomez AC,Pasek DA,Meissner G,Yamaguchi N

    更新日期:2017-09-01 00:00:00

  • Sodium and ouabain induce proliferation of rat thymic lymphocytes via calcium- and magnesium- dependent reactions.

    abstract::When the concentrations of either calcium or of magnesium in the culture medium were increased from the normal 0.6 and 1.0 mM to 1.8 and 2.5 mM respectively mitotic activity of rat thymic lymphocytes increased. Very high (10(-4)M) ouabain concentrations abolished these mitogenic actions whilst lower (10(-7) and 10(-11...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Atkinson MJ,Cade C,Perris AD

    更新日期:1983-02-01 00:00:00

  • Pancreastatin inhibits insulin secretion in RINm5F cells through obstruction of G-protein mediated, calcium-directed exocytosis.

    abstract::To elucidate the regulatory pathway through which pancreastatin inhibits insulin secretion, RINm5F insulinoma cells were challenged with physiological and pharmacological probes known to stimulate insulin release through different mechanisms. Utilizing the electrophysiological technique of capacitance measurements as ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Hertelendy ZI,Patel DG,Knittel JJ

    更新日期:1996-02-01 00:00:00

  • A comparison of fluorescent Ca²⁺ indicators for imaging local Ca²⁺ signals in cultured cells.

    abstract::Localized subcellular changes in Ca(2+) serve as important cellular signaling elements, regulating processes as diverse as neuronal excitability and gene expression. Studies of cellular Ca(2+) signaling have been greatly facilitated by the availability of fluorescent Ca(2+) indicators. The respective merits of differe...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Lock JT,Parker I,Smith IF

    更新日期:2015-12-01 00:00:00

  • Expression of a truncated form of inositol 1,4,5-trisphosphate receptor type III in the cytosol of DT40 triple inositol 1,4,5-trisphosphate receptor-knockout cells.

    abstract::In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel playing a major role in Ca2+ signaling. Three isoforms of IP3R have been identified and most cell types express different proportions of each isoform. The DT40 B lymphocyte cell line lacking all three IP3R isoform...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Guillemette J,Caron AZ,Regimbald-Dumas Y,Arguin G,Mignery GA,Boulay G,Guillemette G

    更新日期:2005-02-01 00:00:00

  • Transient receptor potential (TRP) channels as molecular targets in lung toxicology and associated diseases.

    abstract::The lungs as the gateways of our body to the external environment are essential for gas exchange. They are also exposed to toxicants from two sides, the airways and the vasculature. Apart from naturally produced toxic agents, millions of human made chemicals were produced since the beginning of the industrial revoluti...

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Dietrich A,Steinritz D,Gudermann T

    更新日期:2017-11-01 00:00:00

  • Synchronized calcium spiking resulting from spontaneous calcium action potentials in monolayers of NRK fibroblasts.

    abstract::The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: de Roos AD,Willems PH,Peters PH,van Zoelen EJ,Theuvenet AP

    更新日期:1997-09-01 00:00:00

  • Distinct Ca2+ response patterns in human natural killer cells during induction of necrosis or apoptosis of target cells.

    abstract::Human CD3-, CD16+ natural killer cells (NKs) induced necrosis in the cell line K562 and the minor population of MOLT-4, while induced apoptosis in the majority of MOLT-4, in one-to-one cell interaction. Simultaneous Ca2+ imaging of NK and target cells revealed remarkable differences in changes of cytosolic Ca2+ concen...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Honda Y,Miyazaki S

    更新日期:1996-04-01 00:00:00

  • Emerging structural biology of TRPM subfamily channels.

    abstract::TRPM family (Transient receptor potential channels, M for melastatin) is a group of intrinsic plasma membrane ion channels which are widely expressed throughout human body. It has been identified as a potent entry point of working desperate diseases out in a new way with newfangled ideas and safer technological means....

    journal_title:Cell calcium

    pub_type: 杂志文章,评审


    authors: Chen Y,Zhang X,Yang T,Bi R,Huang Z,Ding H,Li J,Zhang J

    更新日期:2019-05-01 00:00:00

  • LY294002, but not wortmannin, increases intracellular calcium and inhibits calcium transients in bovine and human airway smooth muscle cells.

    abstract::To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Ethier MF,Madison JM

    更新日期:2002-07-01 00:00:00

  • A role for the intracellular protease calpain in the activation of store-operated calcium entry in human platelets.

    abstract::Here, we report a novel role for the cysteine protease calpain in store-operated calcium entry. Several structurally and mechanistically unrelated inhibitors of calpain inhibited Ca2+ entry activated in human platelets by thapsigargin-evoked Ca2+ store depletion or the physiological agonist thrombin, whereas inhibitor...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Harper AG,Sage SO

    更新日期:2007-02-01 00:00:00

  • Okadaic acid uncouples calcium entry from depletion of intracellular stores.

    abstract::The mechanism by which the depletion of intracellular Ca2+ stores stimulates Ca2+ influx is poorly understood. However, the coupling of depletion to influx is broken during mitosis [Preston, S.F. et. al., (1991) Cell Regul., 2, 915-925]. Thus, in interphase HeLa cells, activation of the histamine H1 receptor, or incub...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Berlin RD,Preston SF

    更新日期:1993-05-01 00:00:00

  • Mitochondria respond to Ca2+ already in the submicromolar range: correlation with redox state.

    abstract::Rapid formation of high-Ca2+ perimitochondrial cytoplasmic microdomains has been shown to evoke mitochondrial Ca2+ signal and activate mitochondrial dehydrogenases, however, the significance of submicromolar cytoplasmic Ca2+ concentrations in the control of mitochondrial metabolism has not been sufficiently elucidated...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Pitter JG,Maechler P,Wollheim CB,Spät A

    更新日期:2002-02-01 00:00:00

  • Mitochondria as the decision makers for cancer cell fate: from signaling pathways to therapeutic strategies.

    abstract::As pivotal players in cellular metabolism, mitochondria have a double-faceted role in the final decision of cell fate. This is true for all cell types, but it is even more important and intriguing in the cancer setting. Mitochondria regulate cell fate in many diverse ways: through metabolism, by producing ATP and othe...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Genovese I,Vezzani B,Danese A,Modesti L,Vitto VAM,Corazzi V,Pelucchi S,Pinton P,Giorgi C

    更新日期:2020-12-01 00:00:00

  • Characterization of an oxytocin-induced rise in [Ca2+]i in single human myometrium smooth muscle cells.

    abstract::The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Holda JR,Oberti C,Perez-Reyes E,Blatter LA

    更新日期:1996-07-01 00:00:00

  • Inositol 1,4,5-trisphosphate binding sites copurify with the putative Ca-storage protein calreticulin in rat liver.

    abstract::Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Enyedi P,Szabadkai G,Krause KH,Lew DP,Spät A

    更新日期:1993-06-01 00:00:00

  • Chronic fluoxetine administration increases expression of the L-channel gene Cav1.2 in astrocytes from the brain of treated mice and in culture and augments K(+)-induced increase in [Ca(2+)]i.

    abstract::We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Du T,Liang C,Li B,Hertz L,Peng L

    更新日期:2014-03-01 00:00:00

  • Calmodulin-dependent Ca2+-pump ATPase of human smooth muscle sarcolemma.

    abstract::An enzymatically active Ca2+-stimulated ATPase has been isolated from the sarcolemmal sheets of human smooth muscle (myometrium). Ca2+-ATPase activity was quantitated in an assay medium which simulated the characteristic free ionic concentrations of the cytosol. New computer programs for calculating the composition of...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Popescu LM,Ignat P

    更新日期:1983-10-01 00:00:00

  • Impairment of myocardial calcium homeostasis by antibodies against the adenine nucleotide translocator.

    abstract::The adenine nucleotide translocator (ANT) is an autoantigen in myocarditis and dilated cardiomyopathy. Carrier-specific antibodies impair myocardial energy metabolism and heart function. They cross-react with a myolemmal calcium channel and alter calcium fluxes in isolated myocytes. To test whether antibodies against ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Schulze K,Heineman FW,Schultheiss HP,Balaban RS

    更新日期:1999-05-01 00:00:00

  • Mitochondrial free [Ca2+] levels and the permeability transition.

    abstract::Mitochondrial Ca(2+) activates many processes, from mitochondrial metabolism to opening of the permeability transition pore (PTP) and apoptosis. However, there is considerable controversy regarding the free mitochondrial [Ca(2+)] ([Ca(2+)](M)) levels that can be attained during cell activation or even in mitochondrial...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Vay L,Hernández-SanMiguel E,Lobatón CD,Moreno A,Montero M,Alvarez J

    更新日期:2009-03-01 00:00:00

  • Endogenous cardiotonic steroids and cardiovascular disease, where to next?

    abstract::Ever since British Physician William Withering first described the use of foxglove extract for treatment of patients with congestive heart failure in 1785, cardiotonic steroids have been used clinically to treat heart failure and more recently atrial fibrillation. Due to their ability to bind and inhibit the ubiquitou...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Pavlovic D

    更新日期:2020-03-01 00:00:00

  • The effect of Ca2+ and calmodulin on the inhibition of Ca2(+)+Mg2(+)-ATPase in erythrocyte ghost membranes by nonpolar and polar carbodiimides.

    abstract::N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Famulski KS,Pikula S,Wrzosek A,Wojtczak AB

    更新日期:1990-04-01 00:00:00

  • Intracellular concentrations of fura-2 and fura-2/am in vascular smooth muscle cells following perfusion loading of fura-2/am in arterial segments.

    abstract::A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Tran NN,Leroy P,Bellucci L,Robert A,Nicolas A,Atkinson J,Capdeville-Atkinson C

    更新日期:1995-11-01 00:00:00

  • In vivo labelling of L-type Ca2+ channels by fluorescent dihydropyridine: correlation between ontogenesis of the channels and the acquisition of neural competence in ecotderm cells from Pleurodeles waltl embryos.

    abstract::The ability of the ectodermal cells to be induced and to differentiate toward neural tissue, called neural competence, is acquired shortly before gastrulation and lost during late gastrula stages in Pleurodeles waltl embryos. We have examined ectodermal cells' neural competence in relation to the evolution of the dens...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Leclerc C,Duprat AM,Moreau M

    更新日期:1995-03-01 00:00:00

  • Two dye two wavelength excitation calcium imaging: results from bovine adrenal chromaffin cells.

    abstract::We tested a mixture of Calcium-Green-1 (CG-1) and Brilliantsulfaflavine (BS) for dual excitation ratiometric measurements of the intracellular free calcium concentration ([Ca2+]i) in bovine adrenal chromaffin cells. Dyes were coloaded (without being molecularly linked to each other) in the whole-cell configuration of ...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Oheim M,Naraghi M,Müller TH,Neher E

    更新日期:1998-07-01 00:00:00

  • Examination of the role of TRPM8 in human mast cell activation and its relevance to the etiology of cold-induced urticaria.

    abstract::Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Medic N,Desai A,Komarow H,Burch LH,Bandara G,Beaven MA,Metcalfe DD,Gilfillan AM

    更新日期:2011-11-01 00:00:00

  • A ryanodine-sensitive calcium store in ascidian eggs monitored by whole-cell patch-clamp recordings.

    abstract::Using whole cell patch clamp recordings on unfertilized eggs of the ascidian Ciona intestinalis, we are able to detect ryanodine receptors within the oocytes. Our approach is based on measurements of the voltage-activated inward calcium currents. Two types of Ca2+ currents have been described on the oocyte membrane of...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Arnoult C,Albrieux M,Antoine AF,Grunwald D,Marty I,Villaz M

    更新日期:1997-02-01 00:00:00

  • The effect of furosemide on calcium ion concentration in myocardial cells.

    abstract::The effect of furosemide and ouabain on the intracellular concentration of Ca2+ was studies in myocardial cell cultures using Fura-2, a fluorescent agent, as an intracellular Ca2+ indicator. Introduction of 200 microM ouabain to the cultured cells increased the intracellular calcium concentration from an average of 23...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Rubin Y,Kessler-Icekson G,Navon G

    更新日期:1995-08-01 00:00:00

  • Identification of tyrosines in the putative regulatory site of the Ca2+ channel TRPV6.

    abstract::In HEK293 cells, transfected with the Ca2+ channel protein TRPV6, Ca2+ influx is increased and TRPV6 is tyrosine phosphorylated following addition of the tyrosine phosphatase inhibitor N,N-dimethyl-hydroxamido hydroxovanadate to cells. This effect of DMHV is enhanced by co-transfection of cells with the tyrosine kinas...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Sternfeld L,Anderie I,Schmid A,Al-Shaldi H,Krause E,Magg T,Schreiner D,Hofer HW,Schulz I

    更新日期:2007-07-01 00:00:00

  • The calcium release channel of sarcoplasmic reticulum is modulated by FK-506 binding protein: effect of FKBP-12 on single channel activity of the skeletal muscle ryanodine receptor.

    abstract::The calcium release channel/ryanodine receptor of rabbit skeletal muscle sarcoplasmic reticulum is tightly associated with the immunophilin FK-506 binding protein (FKBP-12). The immunosuppressant drug FK-506 effectively dissociates FKBP-12 from the calcium release channel of terminal cisternae (TC) vesicles. Furthermo...

    journal_title:Cell calcium

    pub_type: 杂志文章


    authors: Mayrleitner M,Timerman AP,Wiederrecht G,Fleischer S

    更新日期:1994-02-01 00:00:00