In vivo labelling of L-type Ca2+ channels by fluorescent dihydropyridine: correlation between ontogenesis of the channels and the acquisition of neural competence in ecotderm cells from Pleurodeles waltl embryos.

abstract：:Factors contributing to "local control" of Ca2+ release in cardiac myocytes are incompletely understood. We induced local release of Ca2+ by regional exposure of mouse atrial and ventricular myocytes to 10mM caffeine for 500 ms using a rapid solution switcher. Propagation of Ca2+ release was imaged by means of a Nipko...

journal_title：Cell calcium

authors： Seguchi H,Ritter M,Shizukuishi M,Ishida H,Chokoh G,Nakazawa H,Spitzer KW,Barry WH

更新日期：2005-07-01 00:00:00

abstract：:There is growing evidence indicating that the pore structure of voltage-gated ion channels (VGICs) influences gating besides their conductance. Regarding low voltage-activated (LVA) Ca2+ channels, it has been demonstrated that substitutions of the pore aspartate (D) by a glutamate (D-to-E substitution) in domains III ...

journal_title：Cell calcium

authors： Garza-López E,Aldana A,Darszon A,Nishigaki T,López-González I

更新日期：2020-07-01 00:00:00

abstract：:Changes in cytosolic free Ca(2+) concentration ([Ca(2+)]i) were monitored optically in hair cells mechanically isolated from frog semicircular canals using the membrane-impermeant form of the Ca(2+)-selective dye Oregon Green 488 BAPTA-1 (OG, 100 microM). Cells stimulated by depolarization under whole-cell voltage cla...

journal_title：Cell calcium

authors： Rispoli G,Martini M,Rossi ML,Mammano F

更新日期：2001-08-01 00:00:00

abstract：:There is controversy as to whether TRP channels participate in mediating store-operated current (I(SOC)) and store-operated Ca(2+) entry (SOCE). Our recent study has demonstrated that TRPC1 forms heteromeric channels with TRPV4 in vascular endothelial cells and that Ca(2+) store depletion enhances the vesicle traffick...

journal_title：Cell calcium

authors： Ma X,Cheng KT,Wong CO,O'Neil RG,Birnbaumer L,Ambudkar IS,Yao X

更新日期：2011-12-01 00:00:00

abstract：:Intracellular Ca(2+)-transport ATPases exert a pivotal role in the endoplasmic reticulum and in the compartments of the cellular secretory pathway by maintaining a sufficiently high lumenal Ca(2+) (and Mn(2+)) concentration in these compartments required for an impressive number of vastly different cell functions. At ...

journal_title：Cell calcium

authors： Wuytack F,Raeymaekers L,Missiaen L

更新日期：2002-11-01 00:00:00

abstract：:The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...

journal_title：Cell calcium

authors： Holda JR,Oberti C,Perez-Reyes E,Blatter LA

更新日期：1996-07-01 00:00:00

abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...

journal_title：Cell calcium

authors： Barclay JW,Morgan A,Burgoyne RD

更新日期：2005-09-01 00:00:00

abstract：:We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...

journal_title：Cell calcium

authors： Elhamdani A,Bossu JL,Feltz A

更新日期：1994-11-01 00:00:00

abstract：:Atypical absence epilepsy (AAE) is an intractable disorder characterized by slow spike-and-wave discharges in electroencephalograms (EEGs) and accompanied by severe cognitive dysfunction and neurodevelopmental or neurological deficits in humans. Administration of the cholesterol biosynthesis inhibitor AY-9944 (AY) dur...

journal_title：Cell calcium

authors： Jung S,Jeong Y,Jeon D

更新日期：2015-05-01 00:00:00

abstract：:Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...

journal_title：Cell calcium

authors： Razani-Boroujerdi S,Partridge LD,Sopori ML

更新日期：1994-12-01 00:00:00

abstract：:Ca2+ influx into secretory cells of the intact salivary gland of the blowfly Calliphora erythrocephala elicited by the agonist 5-hydroxytryptamine (5-HT) or the Ca2+ uptake inhibitor thapsigargin was studied by using Fura-2 and digital fluorescence imaging and by recordings of the transepithelial potential. Applicatio...

journal_title：Cell calcium

authors： Zimmermann B

更新日期：1998-01-01 00:00:00

abstract：:Cultured human skeletal muscle cells are frequently used as a model to study muscle pathology, in which Ca2+ homeostasis might be affected. However, their excitation-contraction (E-C) coupling has been poorly investigated. In order to elucidate E-C coupling of cultured muscle cells, we activated the acetylcholine rece...

journal_title：Cell calcium

authors： Benders AA,Oosterhof A,Wevers RA,Veerkamp JH

更新日期：1997-01-01 00:00:00

abstract：:Although disturbance of cardiac Ca2+ regulation is involved in the pathophysiology of iron-overload cardiomyopathy, the obvious mechanisms involved in the dysregulation of iron-induced cardiac Ca2+ are unclear. Moreover, the roles of the iron chelator deferiprone and the T-type calcium channel blocker efonidipine on c...

journal_title：Cell calcium

authors： Khamseekaew J,Kumfu S,Palee S,Wongjaikam S,Srichairatanakool S,Fucharoen S,Chattipakorn SC,Chattipakorn N

更新日期：2018-06-01 00:00:00

abstract：:The regulation of intracellular Ca(2+) signalling by phosphorylation processes remains poorly defined, particularly with regards to tyrosine phosphorylation. Evidence from non-excitable cells implicates tyrosine phosphorylation in the activation of so-called store-operated Ca(2+) channels (SOCCs), but their involvemen...

journal_title：Cell calcium

authors： Koss DJ,Riedel G,Platt B

更新日期：2009-07-01 00:00:00

abstract：:K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ +...

journal_title：Cell calcium

authors： Jalloul AH,Liu G,Szerencsei RT,Schnetkamp PPM

更新日期：2018-09-01 00:00:00

abstract：:Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...

journal_title：Cell calcium

authors： Oiki S,Yamamoto T,Okada Y

更新日期：1994-03-01 00:00:00

abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...

journal_title：Cell calcium

authors： Putney JW Jr

更新日期：2003-10-01 00:00:00

abstract：:The highly differentiated structure of the neuron poses special problems for the intracellular movement of molecules throughout the cell. Molecular transport distances from the synthesizing neuron cell body along the axon (which has no substantial synthetic capabilities) to the axon terminal are very great. The transp...

journal_title：Cell calcium

authors： Breuer AC,Atkinson MB

更新日期：1988-12-01 00:00:00

abstract：:The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...

journal_title：Cell calcium

authors： Tiffert T,Lew VL

更新日期：2001-11-01 00:00:00

abstract：:Ryanodine receptors plays a crucial role in skeletal muscle excitation-contraction coupling by releasing calcium ions required for muscle contraction from the sarcoplasmic reticulum. At least three phenotypes associated with more than 100 RYR1 mutations have been identified; in order to elucidate possible pathophysiol...

journal_title：Cell calcium

authors： Ghassemi F,Vukcevic M,Xu L,Zhou H,Meissner G,Muntoni F,Jungbluth H,Zorzato F,Treves S

更新日期：2009-02-01 00:00:00

abstract：:An inward current responsible for hormone regulated Ca2+ entry has been identified in cultured rat hepatocytes using whole cell patch clamp. Addition of 20 nM vasopressin or of 100 microM ATP induced the inward current, which could be observed more clearly after blocking an outward K+ current. This large outward K+ cu...

journal_title：Cell calcium

authors： Duszynski J,Elensky M,Cheung JY,Tillotson DL,LaNoue KF

更新日期：1995-07-01 00:00:00

abstract：:A fluid stream induced by a concentration clamp system evokes in Xenopus oocytes a deformation of the membrane which results in transient chloride currents of high amplitude (stream-evoked inward current, I(i,st)) during calcium-activated chloride current oscillations. The involvement of cytoskeleton elements and of c...

journal_title：Cell calcium

authors： Montag S,Krüger K,Madeja M,Speckmann EJ,Musshoff U

更新日期：2004-04-01 00:00:00

abstract：:Rat liver was homogenized and subjected to differential centrifugation. When the low speed nuclear pellet was processed on a Percoll gradient, plasma membrane markers and Ins(1,4,5)P3 binding activity purified together. The high speed (microsomal) fraction was subfractionated by sucrose density gradient centrifugation...

journal_title：Cell calcium

authors： Enyedi P,Szabadkai G,Krause KH,Lew DP,Spät A

更新日期：1993-06-01 00:00:00

abstract：:In non-excitable cells, the inositol 1,4,5-trisphosphate receptor (IP3R) is an intracellular Ca2+ channel which plays a major role in Ca2+ signalling. Three isoforms of IP3R have been identified (IP3R-1, IP3R-2 and IP3R-3) and most cell types express different proportions of each isoform. The differences between the p...

journal_title：Cell calcium

authors： Caron AZ,Chaloux B,Arguin G,Guillemette G

更新日期：2007-09-01 00:00:00

abstract：:The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...

journal_title：Cell calcium

authors： de Roos AD,Willems PH,Peters PH,van Zoelen EJ,Theuvenet AP

更新日期：1997-09-01 00:00:00

abstract：:The high capacity, low affinity Ca(2+) storage protein chromogranins are marker proteins of secretory granules that contain the most Ca(2+) in secretory cells. Along with the abundantly expressed chromogranins, the IP(3)R/Ca(2+) channels, the major intracellular Ca(2+) channels, are also expressed in secretory granule...

journal_title：Cell calcium

authors： Yoo SH,Hur YS

更新日期：2012-03-01 00:00:00

abstract：:The spatiotemporal dynamics of elementary Ca(2+) release events, such as "blips" and "puffs" shapes the hierarchal Ca(2+) signaling in many cell types. Despite being the building blocks of Ca(2+) patterning, the mechanism responsible for the observed properties of puffs, especially their termination is incompletely un...

journal_title：Cell calcium

authors： Ullah G,Parker I,Mak DO,Pearson JE

更新日期：2012-08-01 00:00:00

abstract：:Near-membrane [Ca2+] may differ significantly from bulk cytosolic [Ca2+], particularly during rapid Ca2+ signalling events related to cardiac muscle excitation-contraction coupling. We used the lipophilic membrane-associated Ca2+ indicator Ca(2+)-Green C-18 (C-18) and laser-scanning confocal microscopy to detect extra...

journal_title：Cell calcium

authors： Blatter LA,Niggli E

更新日期：1998-05-01 00:00:00

abstract：:Light-sensitive channels encoded by the Drosophila transient receptor potential-like gene (trpl) are activated in situ by an unknown mechanism requiring activation of Gq and phospholipase C (PLC). Recent studies have variously concluded that heterologously expressed TRPL channels are activated by direct Gq-protein int...

journal_title：Cell calcium

authors： Hardie RC,Raghu P

更新日期：1998-09-01 00:00:00

abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...

journal_title：Cell calcium

authors： Harrington MA,Thompson SH

更新日期：1996-05-01 00:00:00