Abstract：
:There is controversy as to whether TRP channels participate in mediating store-operated current (I(SOC)) and store-operated Ca(2+) entry (SOCE). Our recent study has demonstrated that TRPC1 forms heteromeric channels with TRPV4 in vascular endothelial cells and that Ca(2+) store depletion enhances the vesicle trafficking of heteromeric TRPV4-C1 channels, causing insertion of more channels into the plasma membrane in vascular endothelial cells. In the present study, we determined whether the enhanced TRPV4-C1 insertion to the plasma membrane could contribute to SOCE and I(SOC). We found that thapsigargin-induced SOCE was much lower in aortic endothelial cells derived from trpv4(-/-) or trpc1(-/-) knockout mice when compared to that of wild-type mice. In human umbilical vein endothelial cells (HUVECs), thapsigargin-induced SOCE was markedly reduced by knocking down the expression of TRPC1 and/or TRPV4 with respective siRNAs. Brefeldin A, a blocker of vesicular translocation, inhibited the SOCE. These results suggest that an enhanced vesicular trafficking of heteromeric TRPV4-C1 channels contributes to SOCE in vascular endothelial cells. Vascular tension studies suggest that such an enhanced trafficking of TRPV4-C1 channels may play a role in thapsigargin-induced vascular relaxation in rat small mesenteric arteries.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Ma X,Cheng KT,Wong CO,O'Neil RG,Birnbaumer L,Ambudkar IS,Yao Xdoi
10.1016/j.ceca.2011.08.006subject
Has Abstractpub_date
2011-12-01 00:00:00pages
502-9issue
6eissn
0143-4160issn
1532-1991pii
S0143-4160(11)00170-9journal_volume
50pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Synaptic transmission is a key signaling event, whereby an action potential-induced release of chemical neurotransmitters again generates a positive or negative electrical activity via opening of postsynaptic channels. Thereafter, information spreads through space, from the postsynaptic membranes to the dendrites, to ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(98)90113-0
更新日期：1998-02-01 00:00:00
abstract：:Burst firing of the thalamic neurons is driven by the low threshold Ca2+ spike generated by Ca2+ influx through T-type Ca2+ channels when these channels are activated by membrane hyperpolarization due to inhibitory inputs. The major inhibitory inputs to the thalamocortical (TC) neurons are from the GABAergic neurons i...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.023
更新日期：2006-08-01 00:00:00
abstract：:An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90070-5
更新日期：1989-04-01 00:00:00
abstract：:TRP family of proteins are components of unique cation channels that are activated in response to diverse stimuli ranging from growth factor and neurotransmitter stimulation of plasma membrane receptors to a variety of chemical and sensory signals. This review will focus on members of the TRPC sub-family (TRPC1-TRPC7)...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.011
更新日期：2006-11-01 00:00:00
abstract：:Calcium regulates the proliferation and differentiation of keratinocytes both in vivo and in vitro. Elevated extracellular Ca2+ concentration ([Ca2+]o) raises the intracellular free calcium ([Ca2+]i) and activates differentiation-related genes. Cells lacking the calcium-sensing receptor (CaR) fail to respond to [Ca2+]...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2003.10.019
更新日期：2004-03-01 00:00:00
abstract：:Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00082-4
更新日期：2003-08-01 00:00:00
abstract：:We have recently shown that freshly isolated astrocytes from the mouse brain express mRNA for the L-channel gene Cav1.3 to at least the same degree (per mg mRNA) as corresponding neurons. The amount of extracellular Ca(2+) actually entering cultured astrocytes by its opening is modest, but due to secondary Ca(2+)-medi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.01.002
更新日期：2014-03-01 00:00:00
abstract：:Studies were conducted to examine the possible involvement of Na+-Ca2+ exchanger in determining the magnitude of the endothelin-1 (ET-1)-receptor-mediated calcium signal in porcine lens epithelial cells. Cytoplasmic calcium concentration was measured in primary cultured cells loaded with Fura-2. ET-1 (100 nM) caused c...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00085-x
更新日期：2003-09-01 00:00:00
abstract：:Sarcoplasmic reticulum (SR) is abundant in uterine smooth muscle cells. The functional role of this organelle in the regulation of uterine myocytes is not fully understood. The data available in the literature suggest that SR plays a dual role: as a source of calcium and as a calcium sink shaping calcium transients pr...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.01.006
更新日期：2004-06-01 00:00:00
abstract：:Several recent reports have demonstrated how engineered proteins can control cell motility, an important functional module for ultimately programming cells as therapeutics. We have reported two engineered proteins that regulate the blebbing cell morphology using chimeras of RhoA, a protein that regulates cytoskeletal ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.12.005
更新日期：2013-03-01 00:00:00
abstract：:A whole range of cell functions are regulated by the free cytosolic Ca(2+)concentration. Activator Ca(2+)from the extracellular space enters the cell through various types of Ca(2+)channels and sometimes the Na(+)/Ca(2+)-exchanger, and is actively extruded from the cell by Ca(2+)pumps and Na(+)/Ca(2+)-exchangers. Acti...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1054/ceca.2000.0131
更新日期：2000-07-01 00:00:00
abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90113-x
更新日期：1996-05-01 00:00:00
abstract：:The measurement of intracellular calcium ion concentrations [( Ca2+]i) in single living cells using quantitative fluorescence microscopy draws from a diverse set of disciplines, including cellular biology, optical physics, statistics and computer science. Over the last few years, we have devised and built a number of ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(90)90068-6
更新日期：1990-02-01 00:00:00
abstract：:The endoplasmic reticulum (ER) and mitochondria cannot be considered as static structures, as they intimately communicate, forming very dynamic platforms termed mitochondria-associated membranes (MAMs). In particular, the ER transmits proper Ca2+ signals to mitochondria, which decode them into specific inputs to regul...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.05.003
更新日期：2018-01-01 00:00:00
abstract：:Incubation of human erythrocyte ghosts with an equal volume of 0.2 mM EDTA in isotonic KCl decreased both the activity and Ca2+ sensitivity of the (Ca2+ + Mg2+)-ATPase remaining associated with the membrane. Readdition of the EDTA-extract activated the (Ca2+ + Mg2+)-ATPase activity. The activator activity was trypsin ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(84)90156-8
更新日期：1984-02-01 00:00:00
abstract：:Calcium entry from the extracellular space into cells is an important signaling mechanism in both physiological and pathophysiological functions. In non-excitable cells, store-operated calcium (SOC) entry represents a principal mode of calcium entry. Activation of SOC entry in pulmonary artery endothelial cells leads ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.12.008
更新日期：2013-04-01 00:00:00
abstract：:Ever since British Physician William Withering first described the use of foxglove extract for treatment of patients with congestive heart failure in 1785, cardiotonic steroids have been used clinically to treat heart failure and more recently atrial fibrillation. Due to their ability to bind and inhibit the ubiquitou...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102156
更新日期：2020-03-01 00:00:00
abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00143-x
更新日期：2003-10-01 00:00:00
abstract：:Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.05.008
更新日期：2013-09-01 00:00:00
abstract：:The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) mediates increases in the intracellular concentration of Ca(2+) ([Ca(2+)]i) during fertilization in mammalian eggs. The activity of IP3R1 is enhanced during oocyte maturation, and phosphorylations by M-phase kinases are thought to positively regulate the activit...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.07.004
更新日期：2015-11-01 00:00:00
abstract：:Calbindin D28K (formerly known as vitamin D-dependent calcium binding protein and referred to here as calbindin) is found in a wide variety of tissues, but only in certain cells within those tissues. Apart from its ability to bind calcium, nothing is known about its function in these cells. To investigate its role we ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90055-b
更新日期：1993-04-01 00:00:00
abstract：:The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90013-0
更新日期：1997-09-01 00:00:00
abstract：:The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2011.06.001
更新日期：2011-10-01 00:00:00
abstract：:Adipokinetic hormone (AKH)-induced mobilization of insect fat body glycogen occurs through activation of glycogen phosphorylase. In the migratory locust, signal transduction of AKH-I, -II and -III has been shown to involve the formation of cAMP. In the present study, we show that both the elevation of fat body cAMP le...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90092-6
更新日期：1995-06-01 00:00:00
abstract：:One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid met...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.12.008
更新日期：2017-06-01 00:00:00
abstract：:Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.05.003
更新日期：2014-08-01 00:00:00
abstract：:Using single-cell ratio imaging of Fura-2-loaded neutrophils, we demonstrate that the heterogeneity and asynchrony of the oxidase response originates from variability in the timing and magnitude of the cytosolic free Ca2+ signal. The Ca2+ signals from individual cells could be classified into four types: (a) type 1, a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90020-u
更新日期：1990-11-01 00:00:00
abstract：:The mammalian transient receptor potential (TRP) proteins consist of a superfamily of Ca2+-permeant non-selective cation channels with structural similarities to Drosophila TRP. The TRP superfamily can be divided into three major families, among them the "canonical TRP" family (TRPC). The seven protein products of the...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00056-3
更新日期：2003-05-01 00:00:00
abstract：:A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90027-6
更新日期：1995-10-01 00:00:00
abstract：:Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be pr...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.02.001
更新日期：2011-03-01 00:00:00
