Abstract:
:Amyloid beta (Aβ) oligomers accumulate in the brain tissue of Alzheimer disease patients and are related to disease pathogenesis. The precise mechanisms by which Aβ oligomers cause neurotoxicity remain unknown. We recently reported that Aβ oligomers cause intracellular Ca(2+) overload and neuronal death that can be prevented by NMDA receptor antagonists. This study investigated whether Aβ oligomers directly activated NMDA receptors (NMDARs) using NR1/NR2A and NR1/NR2B receptors that were heterologously expressed in Xenopus laevis oocytes. Indeed, Aβ oligomers induced inward non-desensitizing currents that were blocked in the presence of the NMDA receptor antagonists memantine, APV, and MK-801. Intriguingly, the amplitude of the responses to Aβ oligomers was greater for NR1/NR2A heteromers than for NR1/NR2B heteromers expressed in oocytes. Consistent with these findings, we observed that the increase in the cytosolic concentration of Ca(2+) induced by Aβ oligomers in cortical neurons is prevented by AP5, a broad spectrum NMDA receptor antagonist, but slightly attenuated by ifenprodil which blocks receptors with the NR2B subunit. Together, these results indicate that Aβ oligomers directly activate NMDA receptors, particularly those with the NR2A subunit, and further suggest that drugs that attenuate the activity of such receptors may prevent Aβ damage to neurons in Alzheimeŕs disease.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Texidó L,Martín-Satué M,Alberdi E,Solsona C,Matute Cdoi
10.1016/j.ceca.2011.02.001subject
Has Abstractpub_date
2011-03-01 00:00:00pages
184-90issue
3eissn
0143-4160issn
1532-1991pii
S0143-4160(11)00020-0journal_volume
49pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(02)00060-x
更新日期:2002-06-01 00:00:00
abstract::The spatiotemporal dynamics of elementary Ca(2+) release events, such as "blips" and "puffs" shapes the hierarchal Ca(2+) signaling in many cell types. Despite being the building blocks of Ca(2+) patterning, the mechanism responsible for the observed properties of puffs, especially their termination is incompletely un...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2012.04.018
更新日期:2012-08-01 00:00:00
abstract::Evidence is accumulating on a key role of T-type channels in neurotransmitter release. Recent works have brought undisputable proofs that T-type channels are capable of controlling hormone and neurotransmitters release in association with exocytosis of large dense-core and synaptic vesicles. T-type channel-secretion c...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2006.04.019
更新日期:2006-08-01 00:00:00
abstract::It is well known that in different tissues, dihydropyridines bind at nanomolar concentrations to a receptor and block voltage-operated Ca2+ channels. In studies reported here, Harderian gland tissue homogenates from intact male hamsters exhibited significant dihydropyridine binding (Bmax = 1700 fmoles/mg protein) of h...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90036-r
更新日期:1992-10-01 00:00:00
abstract::The effect of Ca2+ ion concentration on the 25 hydroxylation of tritiated cholecalciferol (3HD3) was investigated using homogenates of ovine liver from vitamin D replete sheep. A significant decrease in the production of 25 hydroxycholecalciferol (25OHD3) was observed when the concentration of Ca2+ in the homogenate w...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(87)90001-7
更新日期:1987-08-01 00:00:00
abstract::We studied the contribution of store-operated or capacitative Ca2+-entry (SOCE or CCE, respectively) through store-operated Ca2+ channels (SOCCs) and the contribution of Ca2+-entry through receptor-operated, non-selective cation channels (ROCCs or NSCCs, respectively), on the M3-receptor-mediated (270 nM Ach) contract...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.06.035
更新日期:2005-11-01 00:00:00
abstract::One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid met...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2016.12.008
更新日期:2017-06-01 00:00:00
abstract::Cardiogenesis depends on a tightly regulated balance between proliferation and differentiation of cardiac progenitor cells (CPCs) and their cardiomyocyte descendants. While exposure of early mouse embryos to Ca(2+) channel antagonists has been associated with abnormal cardiac morphogenesis, less is known about the con...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.02.018
更新日期:2014-05-01 00:00:00
abstract::Sarcoplasmic reticulum contains the internal Ca(2+) store in smooth muscle cells and its lumen appears to be a continuum that lacks diffusion barriers. Accordingly, the free luminal Ca(2+) level is the same all throughout the SR; however, whether the Ca(2+) buffer capacity is the same in all the SR is unknown. We have...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2009.07.005
更新日期:2009-09-01 00:00:00
abstract::We have constructed a stochastic model of the inositol 1,4,5-trisphosphate receptor-Ca2+ channel that is based on quantitative measurements of the channel's properties. It displays the observed dependence of the open probability of the channel with cytosolic [Ca2+] and [IP3] and gives values for the dwell times that a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2003.10.004
更新日期:2004-05-01 00:00:00
abstract::A procedure for calibration of fluorescence signals from adult rat heart cells loaded with the -AM ester of fura-2 is described. Calibration is complicated by dye compartmentation and potentially incomplete dye hydrolysis. These problems were overcome by subtracting from fluorescence transients the non-cytosolic (mito...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90050-1
更新日期:1998-10-01 00:00:00
abstract::Changes in the concentration and spatial distribution of Ca(2+) ions in the cytoplasm constitute a ubiquitous intracellular signaling module in cellular physiology. With the advent of Ca(2+) dyes that allow direct visualization of Ca(2+) transients, combined with powerful experimental tools such as electrophysiologica...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2010.10.003
更新日期:2010-11-01 00:00:00
abstract::Pacemaker cells residing in the sinoatrial node generate the regular heartbeat. Ca2+ signaling controls the heartbeat rate-directly, through the effect on membrane molecules (NCX exchange, K+ channel), and indirectly, through activation of calmodulin-AC-cAMP-PKA signaling. Thus, the physiological role of signaling in ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2018.12.008
更新日期:2019-03-01 00:00:00
abstract::Maitotoxin (MTX), a water-soluble polyether obtained from the marine dinoflagellate Gambierdiscus toxicus increased intracellular calcium in a concentration-dependent manner in fibroblasts obtained from human skin. The effect of this toxin was both saturable and of high affinity, showing an apparent half activation co...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90087-7
更新日期:1997-07-01 00:00:00
abstract::Nanosecond-duration electric stimuli are distinguished by the ability to permeabilize intracellular membranes and recruit Ca2+ from intracellular stores. We quantified this effect in non-excitable cells (CHO) using ratiometric Ca2+ imaging with Fura-2. In a Ca(2+)-free medium, 10-, 60-, and 300-ns stimuli evoked Ca2+ ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2013.05.008
更新日期:2013-09-01 00:00:00
abstract::The calcium release channel/ryanodine receptor of rabbit skeletal muscle sarcoplasmic reticulum is tightly associated with the immunophilin FK-506 binding protein (FKBP-12). The immunosuppressant drug FK-506 effectively dissociates FKBP-12 from the calcium release channel of terminal cisternae (TC) vesicles. Furthermo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90048-5
更新日期:1994-02-01 00:00:00
abstract::In the brain, classical (canonical) transient receptor potential (TRPC) channels are thought to be involved in different aspects of neuronal development. We investigated the developmental expression profile of TRPC channels in rat cerebellum during the first 6 weeks after birth. TRPC3 expression is significantly up-re...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.11.002
更新日期:2007-07-01 00:00:00
abstract::The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(89)90038-9
更新日期:1989-01-01 00:00:00
abstract::Cellular calcium signaling events are transient. Hence they are observed in real time using fluorescence imaging or electrophysiological methods that require sophisticated instrumentation and specialized skills. For high throughput assays simple and inexpensive techniques are desirable. Many calcium channels that serv...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2018.05.009
更新日期:2018-09-01 00:00:00
abstract::TRPA1, a Ca(2+)-permeable cation channel that is expressed in sensory neurones, is involved in the perception of chemical irritants and mechanical hyperalgesia. TRPA1 is activated by either covalent or reversible binding of various chemical compounds, including allylisothiocyanate or acrolein, and is further sensitise...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.08.001
更新日期:2009-02-01 00:00:00
abstract::Ca2+ signals are known to mediate an array of cellular functions including secretion, contraction, and conductivity changes. In spite of the obvious role of Ca2+ in signalling, the control of Ca2+ within cells is known to be a complex phenomenon involving a number of distinct active and passive transport systems funct...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(89)90062-6
更新日期:1989-07-01 00:00:00
abstract::The correlation between the intracellular Ca2+ concentration ([Ca2+]i) and membrane potential in monolayers of density-arrested normal rat kidney (NRK) fibroblasts was investigated. Using the fluorescent probe Fura-2, spontaneous repetitive spike-like increases in [Ca2+]i (Ca2+ spikes) were observed that were synchron...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90013-0
更新日期:1997-09-01 00:00:00
abstract::Dendritic Ca2+ plays an important role not only in synaptic integration and synaptic plasticity, but also in dendritic excitability in midbrain dopamine neurons. However, the functional organization of dendritic Ca2+ signals in the dopamine neurons remains largely unknown. We therefore investigated dendritic Ca2+ sign...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.06.007
更新日期:2011-10-01 00:00:00
abstract::The ability of the ectodermal cells to be induced and to differentiate toward neural tissue, called neural competence, is acquired shortly before gastrulation and lost during late gastrula stages in Pleurodeles waltl embryos. We have examined ectodermal cells' neural competence in relation to the evolution of the dens...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(95)90036-5
更新日期:1995-03-01 00:00:00
abstract::A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.06.012
更新日期:2005-09-01 00:00:00
abstract::Receptor-activated cytoplasmic calcium (Ca2+) oscillations have been investigated in single pancreatic acinar cells by microfluorimetry (Fura-2 as indicator). At submaximal concentrations of the agonists acetylcholine (ACh) and cholecystokinin octapeptide (CCK-8), both give rise to oscillatory changes in the cytosolic...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(91)90016-8
更新日期:1991-02-01 00:00:00
abstract::The release of Ca2+ ions from the sarcoplasmic reticulum through ryanodine receptor calcium release channels represents the critical step linking electrical excitation to muscular contraction in the heart and skeletal muscle (excitation-contraction coupling). Two small Ca2+ binding proteins, S100A1 and calmodulin, hav...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.06.001
更新日期:2011-10-01 00:00:00
abstract::TRPC channels are a subset of the transient receptor potential (TRP) proteins widely expressed in mammalian cells. They are thought to be primarily involved in determining calcium or sodium entry and have broad-ranging functions that include regulation of cell proliferation, motility and contraction. The channels do n...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2009.02.006
更新日期:2009-06-01 00:00:00
abstract::When the concentrations of either calcium or of magnesium in the culture medium were increased from the normal 0.6 and 1.0 mM to 1.8 and 2.5 mM respectively mitotic activity of rat thymic lymphocytes increased. Very high (10(-4)M) ouabain concentrations abolished these mitogenic actions whilst lower (10(-7) and 10(-11...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90044-1
更新日期:1983-02-01 00:00:00
abstract::We have previously demonstrated a role for the reorganization of the actin cytoskeleton in store-operated calcium entry (SOCE) in human platelets and interpreted this as evidence for a de novo conformational coupling step in SOCE activation involving the type II IP(3) receptor and the platelet hTRPC1-containing store-...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.02.004
更新日期:2007-12-01 00:00:00