Abstract：
:We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% within some 10 min. The rundown process was shown not to depend on Ca2+ entry nor on membrane depolarization. It resulted from cell dialysis with a saline solution and, once initiated, it proceeded at a rate of 0.28 min-1 at 4 different Ca2+ concentrations (pCa 5-9). The facilitation is also initiated by cell dialysis but this process developed faster at higher internal Ca2+ concentrations. Thus, globally, high-voltage activated Ca2+ current runs down faster when using a recording pipette solution with a higher internal Ca2+ concentration (pCa 5 or 6). Some leupeptin-sensitive proteases may be involved in the initiation of facilitation and rundown processes.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Elhamdani A,Bossu JL,Feltz Adoi
10.1016/0143-4160(94)90029-9subject
Has Abstractpub_date
1994-11-01 00:00:00pages
357-66issue
5eissn
0143-4160issn
1532-1991pii
0143-4160(94)90029-9journal_volume
16pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:In previous studies we have characterised various properties of capacitative Ca2+ entry (CCE) in different epithelia. After Ca2+ store depletion with PLC/InsP3-coupled agonists or by inhibition of store Ca2+ uptake, with for example thapsigargin, Ca2+ influx is activated. This leads to a sustained cellular response (e...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90112-3
更新日期：1997-08-01 00:00:00
abstract：:Low-voltage-activated T-type calcium channels are expressed in various tissues, especially in the brain, where they promote neuronal firing and are involved in slow wave sleep and absence epilepsy. While the transduction pathways by which hormones and neurotransmitters modulate high-voltage-activated calcium channels ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.015
更新日期：2006-08-01 00:00:00
abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00143-x
更新日期：2003-10-01 00:00:00
abstract：:The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2001.0233
更新日期：2001-10-01 00:00:00
abstract：:The effects of the unsaturated fatty acids, arachidonic and oleic acid, on the influx of Ca2+ activated by depletion of intracellular stores with thapsigargin were investigated in various cell types. By using a Ca2+ free/Ca2+ reintroduction protocol, we observed that arachidonic acid (2 to 5 microM) inhibited thapsiga...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90031-2
更新日期：1997-05-01 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by selective death of motor neurons. Mutations in Cu/Zn superoxide dismutase-1 (SOD1) cause familial ALS but the molecular mechanisms whereby these mutations induce motor neuron death remain controversial. Here, we show that stable overex...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.02.004
更新日期：2011-04-01 00:00:00
abstract：:Hard end is a physiological disorder of pear fruit that is frequently observed in the 'Whangkeumbae' (Pyrus pyrifolia) variety, however, the mechanisms that are involved in its development are poorly understood. In this study, we explored the causes of hard end disorder in pear fruit in relation to calcium deficiency....
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2017.11.002
更新日期：2018-05-01 00:00:00
abstract：:Cultured rat cerebellar astrocytes, loaded with the Ca2+-sensitive fluorescent dyes Fura-2 or Fluo-3, responded with cytoplasmic Ca2+ transients, when the external K+ concentration was reduced from 5 mM to below 1 mM. Ca2+ transients were generated after changing to a saline containing 0.2 mM K+ in 82% of the cells (n...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2000.0153
更新日期：2000-10-01 00:00:00
abstract：:Membrane deformation induced by a mechanical stimulus increases the [Ca2+]i in cultured retinal pigment epithelial (RPE) cells, and in many other cell types. In this study, confocal microscopy and Ca(2+)-measurements using the fluorescent dye fluo-3 were used to measure the spatiotemporal characteristics of the Ca(2+)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0044
更新日期：1999-06-01 00:00:00
abstract：:Calcium and redox signals are presently established as essential regulators of many cellular processes. Nevertheless, we are still far from fully understanding the physiological and pathological importance of these universal second messengers. It is becoming increasingly apparent that many cellular functions are not r...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.04.008
更新日期：2016-08-01 00:00:00
abstract：:Mitochondria from different organisms can undergo a sudden process of inner membrane unselective leakiness to molecules known as the mitochondrial permeability transition (MPT). This process has been studied for nearly four decades and several proteins have been claimed to constitute, or at least regulate the usually ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2018.05.003
更新日期：2018-07-01 00:00:00
abstract：:The mitochondrial permeability transition pore (mPTP) has long been known to have a role in mitochondrial calcium (Ca(2+)) homeostasis under pathological conditions as a mediator of the mitochondrial permeability transition and the activation of the consequent cell death mechanism. However, its role in the context of ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.03.004
更新日期：2014-07-01 00:00:00
abstract：:Agonist-induced calcium (Ca) mobilization is accompanied by Ca efflux, presumably reflecting the rise in Ca concentration at the cytosolic surface of the cell membrane. We studied the relationship between Ca efflux and intracellular Ca mobilization in Xenopus oocytes. Elevation of cytosolic Ca by a direct injection of...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90021-4
更新日期：1996-03-01 00:00:00
abstract：:The A23187 induced calcium uptake in ATP depleted cells was determined at pH 6.9 in the presence of trifluoperazine (TFP, 0.30 mM), compound 48/80 (0.89 mg/ml), 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8, 2.13 mM) and verapamil (1.81 mM). Apart from verapamil the drugs all increased the maximum rate of ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(84)90022-8
更新日期：1984-10-01 00:00:00
abstract：:Specific sequences (cis-acting elements) in the 3'-untranslated region (UTR) of RNA, together with stabilizing and destabilizing proteins (trans-acting factors), determine the mRNA stability, and consequently, the level of expression of several proteins. Such interactions were discovered initially for short-lived mRNA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.004
更新日期：2006-10-01 00:00:00
abstract：:Calcium dependence of bleb formation and cell death was evaluated in rat hepatocytes following ATP depletion by metabolic inhibition with KCN and iodoacetate ('chemical hypoxia'). Cytosolic free Ca2+ was measured in single cells by ratio imaging of Fura-2 fluorescence using multiparameter digitized video microscopy. C...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(88)90004-8
更新日期：1988-12-01 00:00:00
abstract：:The mechanisms that regulate ion and fluid transport by the human intrahepatic bile duct have not been well defined. Human intrahepatic biliary cell lines that we have developed were used to identify and characterize purinoceptors based on increases in intracellular calcium in response to ATP and other nucleotides. In...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90111-6
更新日期：1995-05-01 00:00:00
abstract：:In skeletal muscle excitation-contraction (EC) coupling the sarcolemmal L-type Ca(2+) channel or 1,4-dihydropyridine receptor (DHPR) transduces the membrane depolarization signal to the sarcoplasmic Ca(2+) release channel RyR1 via protein-protein interaction. While it is evident that the pore-forming and voltage-sensi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.04.003
更新日期：2010-06-01 00:00:00
abstract：:Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.08.003
更新日期：2011-11-01 00:00:00
abstract：:Extracellular nucleotides are potent Ca2+ mobilizing agents. A variety of receptors for extracellular ATP are recognised. Some are involved in fast neuronal transmission and operate as ligand-gated ion channels. Others are involved in the paracrine or autocrine modulation of cell function. Many receptors of this type ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90080-2
更新日期：1995-02-01 00:00:00
abstract：:Three decades ago, James W. Putney Jr. conceptualized the idea of store-operated calcium entry (SOCE) to explain how depletion of endoplasmic reticulum (ER) Ca2+ stores evokes Ca2+ influx across the plasma membrane. Since the publication of this highly influential idea, it is now established that SOCE is universal amo...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2016.12.006
更新日期：2017-05-01 00:00:00
abstract：:In murine ventricular myocytes, activation of mechanosensitive ion channels (MSCs) includes activation of non-selective cation currents and deactivation of inwardly rectifying potassium currents. Using pharmacological inhibitors and knockout models, we analyzed signaling steps that are critical to transduce the mechan...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.06.002
更新日期：2009-01-01 00:00:00
abstract：:Human neutrophils, preloaded with the fluorescent probe, Fura-2, were exposed to Ca2+-releasing agents. The monitored traces of fluorescence were transformed by computer to cytosolic Ca2+ concentration ([ Ca2+]i). Due to quenching of Fura-2, the addition of Mn2+ enabled us to compute the cytosolic concentration of tot...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(89)90025-0
更新日期：1989-10-01 00:00:00
abstract：:Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2021.102358
更新日期：2021-01-23 00:00:00
abstract：:The Ca(2+) and cAMP/PKA pathways are the primary signaling systems in secretory epithelia that control virtually all secretory gland functions. Interaction and crosstalk in Ca(2+) and cAMP signaling occur at multiple levels to control and tune the activity of each other. Physiologically, Ca(2+) and cAMP signaling oper...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2014.01.006
更新日期：2014-06-01 00:00:00
abstract：:Cell survival is promoted by the oncoprotein Bcl-2. Previous studies have established that one of the pro-survival actions of Bcl-2 is to reduce cellular fluxes of Ca2+ within cells. In particular, Bcl-2 has been demonstrated to inhibit the release of Ca2+ from the endoplasmic reticulum. However, the mechanism by whic...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.01.003
更新日期：2008-09-01 00:00:00
abstract：:Katz and co-workers showed that Ca(2+) triggers exocytosis. The existence of sub-micrometer domains of greater than 100 microM [Ca(2+)](i) was postulated on theoretical grounds. Using a modified, low-affinity aequorin, Llinas et al. were the first to demonstrate the existence of Ca(2+) 'microdomains' in squid presynap...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.007
更新日期：2006-11-01 00:00:00
abstract：:Animals have evolved sophisticated physiological systems for sensing ambient temperature since changes in environmental temperatures affect various biological processes. Thermosensitive transient receptor potential (thermoTRP) channels serve as thermal sensors in diverse animal species. They are multimodal receptors t...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2014.12.001
更新日期：2015-03-01 00:00:00
abstract：:Several recent reports have demonstrated how engineered proteins can control cell motility, an important functional module for ultimately programming cells as therapeutics. We have reported two engineered proteins that regulate the blebbing cell morphology using chimeras of RhoA, a protein that regulates cytoskeletal ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.12.005
更新日期：2013-03-01 00:00:00
abstract：:Recent developments in microscopy and fluorescent indicators now make it possible to monitor the activity and localization of membrane ion channels by imaging Ca(2+) flux through individual channels. Such optical approaches have advantages over electrophysiological single-channel techniques in that they are less invas...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2004.10.008
更新日期：2005-04-01 00:00:00
