Abstract：
:K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ + 1 K+). In this study, we examined residues in the two regions with the highest degree of homology between the different NCKX isoforms (α-1 and α-2 repeats) to determine which residues are important for Ca2+ coordination. Using fluorescent intracellular Ca2+-indicating dyes, we measured NCKX-mediated Ca2+ transport in HEK293 cells expressing wildtype or mutant NCKX2 and analyzed shifts in the apparent binding affinity (Km) of mutant proteins when compared to the wildtype exchanger. Of the 93 residue substitutions tested, 31 were found to show a significant shift in the external Ca2+ ion dependence of which 18 showed an increased affinity to Ca2+ ions and 13 showed a decreased affinity, and, hence, are believed to be important for Ca2+ ion binding and transport. When compared to the crystal structure of the archaeal Na+-Ca2+ exchanger NCX_Mj and the NCKX2 homology model based on this crystal structure, our biochemical data reveal that these 13 residues are either in direct contact with the Ca2+ ion or lining a Ca2+ transport pathway through the exchanger. Supported by CIHR MOP-81327.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Jalloul AH,Liu G,Szerencsei RT,Schnetkamp PPMdoi
10.1016/j.ceca.2018.06.002subject
Has Abstractpub_date
2018-09-01 00:00:00pages
187-197eissn
0143-4160issn
1532-1991pii
S0143-4160(18)30046-0journal_volume
74pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:The mechanism by which the depletion of intracellular Ca2+ stores stimulates Ca2+ influx is poorly understood. However, the coupling of depletion to influx is broken during mitosis [Preston, S.F. et. al., (1991) Cell Regul., 2, 915-925]. Thus, in interphase HeLa cells, activation of the histamine H1 receptor, or incub...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90042-5
更新日期：1993-05-01 00:00:00
abstract：:Dynamic nuclear Ca(2+) signals play pivotal roles in diverse cellular functions including gene transcription, cell growth, differentiation, and apoptosis. Here we report a novel nuclear Ca(2+) regulatory mechanism mediated by inositol 1,4,5-trisphosphate receptors (IP(3)Rs) around the nucleus in developing cardiac myo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2007.04.017
更新日期：2008-02-01 00:00:00
abstract：:Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00082-4
更新日期：2003-08-01 00:00:00
abstract：:The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(83)90024-6
更新日期：1983-12-01 00:00:00
abstract：:Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90060-4
更新日期：1994-03-01 00:00:00
abstract：:Dopamine is one of the key neurotransmitters in the central nervous system and plays an important role in physiological processes, as well as in the development of many diseases. Here we report a receptor-independent signalling pathway induced by dopamine in CNS neurons. In cultured neurons from midbrain, cortex and h...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.08.008
更新日期：2010-08-01 00:00:00
abstract：:N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(90)90004-e
更新日期：1990-04-01 00:00:00
abstract：:There is growing evidence indicating that the pore structure of voltage-gated ion channels (VGICs) influences gating besides their conductance. Regarding low voltage-activated (LVA) Ca2+ channels, it has been demonstrated that substitutions of the pore aspartate (D) by a glutamate (D-to-E substitution) in domains III ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2020.102214
更新日期：2020-07-01 00:00:00
abstract：:Mathematical models are often used to elucidate mechanisms behind cytosolic Ca2+ oscillations. We have evaluated the use of mathematical modelling to analyse and quantify Ca2+ signal patterns, in single, adherent human neutrophils (PMN) after stimulation by the bacterial peptide N-formyl-methionyl-leucyl-phenylalanine...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90111-2
更新日期：1994-06-01 00:00:00
abstract：:The highly selective fluorescent Ca2+ indicator 'quin 2' has been loaded into ram and boar spermatozoa as the acetoxymethyl ester, 'quin 2/AM', which is hydrolysed and trapped in the cytoplasm. Loadings of several mM were not toxic to spermatozoa as judged by motility. Fluorescence measurements (mean +/- S.E.M.) indic...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(88)90037-1
更新日期：1988-02-01 00:00:00
abstract：:The effect of furosemide and ouabain on the intracellular concentration of Ca2+ was studies in myocardial cell cultures using Fura-2, a fluorescent agent, as an intracellular Ca2+ indicator. Introduction of 200 microM ouabain to the cultured cells increased the intracellular calcium concentration from an average of 23...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90004-7
更新日期：1995-08-01 00:00:00
abstract：:The control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.02.007
更新日期：2008-11-01 00:00:00
abstract：:The effects of the thyroid status on the Ca++-transporting capabilities of rat slow skeletal muscle (m.soleus) were studied. The oxalate supported Ca++-uptake activity and Ca++-loading capacity of muscle homogenates from hyperthyroid rats showed an approximate 4.2 and 2.5 fold increase, respectively, as compared to va...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(86)90018-7
更新日期：1986-06-01 00:00:00
abstract：:A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90027-6
更新日期：1995-10-01 00:00:00
abstract：:A rapid increase in intracellular calcium directly triggers regulated exocytosis. In addition, changes in intracellular calcium concentration can adjust the extent of exocytosis (quantal content) or the magnitude of individual release events (quantal size) in both the short- and long-term. It is generally agreed that ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2005.06.012
更新日期：2005-09-01 00:00:00
abstract：:Cardiac muscle excitation-contraction coupling is controlled by the Ca(2+)-induced Ca2+ release mechanism. The present study examines the effects of a calmodulin antagonist W-7 on Ca2+ current (ICa)-induced Ca2+ release in whole cell-clamped rat ventricular myocytes. Exposure of cells to W-7 suppressed ICa, but the in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1998.0019
更新日期：1999-03-01 00:00:00
abstract：:Here we examine the potential coupling between the synthesis of secretory proteins and the sensitivity of exocytosis to the concentration of free Ca(2+) in the cytosol ([Ca(2+)](i)) in plant cell. We therefore monitor in tobacco protoplasts the excursion of the membrane capacitance in response to an elevation of [Ca(2...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.05.011
更新日期：2012-09-01 00:00:00
abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00143-x
更新日期：2003-10-01 00:00:00
abstract：:In addition to its role in artery contraction, Rho kinase (ROCK) is reported to be involved in the Ca(2+) response to vasoconstrictor agonist in rat aorta. However the signaling pathway mediated by ROCK had not been investigated so far and it was not known whether ROCK also contributed to Ca(2+) signaling in cultured ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.07.002
更新日期：2012-12-01 00:00:00
abstract：:The activation of T lymphocytes, like many cells, requires a rapid rise in intracellular Ca2+ secondary to both an influx and a release from intracellular stores. The latter is activated by inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. It is controversial if inositol phosphates can also stimulate a plasma membrane Ca2+...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90082-h
更新日期：1993-02-01 00:00:00
abstract：:Dendritic Ca2+ plays an important role not only in synaptic integration and synaptic plasticity, but also in dendritic excitability in midbrain dopamine neurons. However, the functional organization of dendritic Ca2+ signals in the dopamine neurons remains largely unknown. We therefore investigated dendritic Ca2+ sign...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.06.007
更新日期：2011-10-01 00:00:00
abstract：:Both theoretical and experimental results are presented for in vivo calibration of the dissociation constant K(Ca)(d)of the calcium-sensitive fluorescent dye Rhod(2)in the perfused mouse heart, using manganese quenching of fluorescence transients. An analytical model is derived, based on the biochemical equilibrium of...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2000.0186
更新日期：2001-04-01 00:00:00
abstract：:The fluo family of indicators is frequently used in studying Ca(2+) physiology; however, choosing which fluo indicator to use is not obvious. Indicator properties are typically determined in well-defined aqueous solutions. Inside cells, however, the properties can change markedly. We have characterized each of three f...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.05.010
更新日期：2012-08-01 00:00:00
abstract：:A new method for the determination of tissue concentrations of Fura-2 and Fura-2/AM was developed based upon acetonitrile extraction followed by RP-HPLC separation (using tetrahexylammonium as counter-ion), post-column alkaline hydrolysis of Fura-2/AM, and fluorimetric detection. The detection limit was 1.2 nM and 1 n...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90057-8
更新日期：1995-11-01 00:00:00
abstract：:Removal of the epithelium from preparations of guinea-pig airways in vitro increases the responsiveness of the smooth muscle of normal and ovalbumin-sensitized animals to a number of contractile agents. To determine if epithelium removal results in an increase in Ca2+ entry into the smooth muscle, the effect of removi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(87)90026-1
更新日期：1987-12-01 00:00:00
abstract：:Neurons generate particular calcium microdomains in response to different stimuli. Calcium microdomains have a central role in a variety of neuronal functions. In particular, calcium microdomains participate in long-lasting synaptic plasticity--a neuronal response presumably correlated with cognitive brain functions t...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.021
更新日期：2006-11-01 00:00:00
abstract：:Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the alpha-subunit of Gq (Ad-EF1-dnG alpha q) and the other expressing the wild-type alpha-subunit of Gq (Ad-EF1-wtG ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90077-x
更新日期：1998-08-01 00:00:00
abstract：:Atypical absence epilepsy (AAE) is an intractable disorder characterized by slow spike-and-wave discharges in electroencephalograms (EEGs) and accompanied by severe cognitive dysfunction and neurodevelopmental or neurological deficits in humans. Administration of the cholesterol biosynthesis inhibitor AY-9944 (AY) dur...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.04.001
更新日期：2015-05-01 00:00:00
abstract：:The importance of Ca2+-signaling for many subcellular processes is well established in higher eukaryotes, whereas information about protozoa is restricted. Recent genome analyses have stimulated such work also with Alveolates, such as ciliates (Paramecium, Tetrahymena) and their pathogenic close relatives, the Apicomp...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.01.006
更新日期：2012-05-01 00:00:00
abstract：:TRPA1, a Ca(2+)-permeable cation channel that is expressed in sensory neurones, is involved in the perception of chemical irritants and mechanical hyperalgesia. TRPA1 is activated by either covalent or reversible binding of various chemical compounds, including allylisothiocyanate or acrolein, and is further sensitise...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.08.001
更新日期：2009-02-01 00:00:00
