Abstract：
:Here we examine the potential coupling between the synthesis of secretory proteins and the sensitivity of exocytosis to the concentration of free Ca(2+) in the cytosol ([Ca(2+)](i)) in plant cell. We therefore monitor in tobacco protoplasts the excursion of the membrane capacitance in response to an elevation of [Ca(2+)](i) as a measure for exocytotic activity. The data show that a ramp like elevation of [Ca(2+)](i) generates in protoplasts from wild type plants and from transgenic plants, which overexpress the secreted α-amylase, an exocytotic burst with an initial steep and a subsequent slow phase. The largest capacitive burst is obtained in α-amylase producing plants and the amplitude of the [Ca(2+)](i) evoked C(m) excursion is a function of the amylase synthesis of the plants. The data support a model according to which plant cells have at least two serial [Ca(2+)](i) sensitive processes in the final steps of their exocytotic pathway. The overproduction of a secreted cargo does not affect the kinetics of this process but the number of vesicles in pools upstream of the [Ca(2+)](i) sensitive steps.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Sutter JU,Denecke J,Thiel Gdoi
10.1016/j.ceca.2012.05.011subject
Has Abstractpub_date
2012-09-01 00:00:00pages
283-8issue
3-4eissn
0143-4160issn
1532-1991pii
S0143-4160(12)00107-8journal_volume
52pub_type
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