Abstract：
:We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Martin VV,Beierlein M,Morgan JL,Rothe A,Gee KRdoi
10.1016/j.ceca.2004.05.002keywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
509-14issue
6eissn
0143-4160issn
1532-1991pii
S0143416004001101journal_volume
36pub_type
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