Abstract：
:Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane attack complex of complement causes a rapid increase in intracellular free Ca2+ concentration before any other detectable biochemical changes in the cell. In nucleated cells the increased intracellular free Ca2+ concentration initially stimulates recovery processes, allowing the cell to escape mild complement attack and also activates the production of inflammatory mediators, which may amplify an ongoing inflammatory response. More severe complement membrane attack causes a more rapid rise in intracellular free Ca2+ concentration allowing a threshold to be breached above which recovery processes are overwhelmed, and cell death occurs. The importance of non-lytic effects and recovery processes mediated by Ca2+, and the molecular basis of these effects are discussed, and the hypothesis proposed that the cell-injuring effects of other "pore-forming" toxins are also caused by increases in intracellular free Ca2+.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Morgan BP,Luzio JP,Campbell AKdoi
10.1016/0143-4160(86)90042-4subject
Has Abstractpub_date
1986-12-01 00:00:00pages
399-411issue
5-6eissn
0143-4160issn
1532-1991pii
0143-4160(86)90042-4journal_volume
7pub_type
杂志文章,评审相关文献
CELL CALCIUM文献大全abstract：:Intercellular Ca(2+)-signaling, after mechanical stimulation of calf pulmonary artery endothelial cells (CPAE), was investigated with fluorescence video imaging. Mechanical stimulation evoked an intracellular Ca(2+)-response in the mechanically stimulated (MS) cell, proceeding to the neighboring (NB) cells as a Ca(2+)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2000.0165
更新日期：2001-02-01 00:00:00
abstract：:Specific sequences (cis-acting elements) in the 3'-untranslated region (UTR) of RNA, together with stabilizing and destabilizing proteins (trans-acting factors), determine the mRNA stability, and consequently, the level of expression of several proteins. Such interactions were discovered initially for short-lived mRNA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.04.004
更新日期：2006-10-01 00:00:00
abstract：:Palmitoylation (S-acylation) is the reversible conjugation of a fatty acid (usually C16 palmitate) to intracellular cysteine residues of proteins via a thioester linkage. Palmitoylation anchors intracellular regions of proteins to membranes because the palmitoylated cysteine is recruited to the lipid bilayer. NCX1 is ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2019.102158
更新日期：2020-03-01 00:00:00
abstract：:In the brain, classical (canonical) transient receptor potential (TRPC) channels are thought to be involved in different aspects of neuronal development. We investigated the developmental expression profile of TRPC channels in rat cerebellum during the first 6 weeks after birth. TRPC3 expression is significantly up-re...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.11.002
更新日期：2007-07-01 00:00:00
abstract：:TRP family of proteins are components of unique cation channels that are activated in response to diverse stimuli ranging from growth factor and neurotransmitter stimulation of plasma membrane receptors to a variety of chemical and sensory signals. This review will focus on members of the TRPC sub-family (TRPC1-TRPC7)...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2006.08.011
更新日期：2006-11-01 00:00:00
abstract：:TRPV6 belongs to the vanilloid family of the transient receptor potential channel (TRP) superfamily. This calcium-selective channel is highly expressed in the duodenum and the placenta, being responsible for calcium absorption in the body and fetus. Previous observations have suggested that TRPV6 is not only permeable...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.11.007
更新日期：2011-01-01 00:00:00
abstract：:Near-membrane [Ca2+] may differ significantly from bulk cytosolic [Ca2+], particularly during rapid Ca2+ signalling events related to cardiac muscle excitation-contraction coupling. We used the lipophilic membrane-associated Ca2+ indicator Ca(2+)-Green C-18 (C-18) and laser-scanning confocal microscopy to detect extra...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90023-9
更新日期：1998-05-01 00:00:00
abstract：:A decrease of cytoplasmic Ca(2+)-concentration in vertebrate photoreceptor cells after illumination is necessary for light adaptation. Although the mechanisms of adaptation is not completely understood, several Ca(2+)-dependent cellular processes have been discovered. Some involve calcium-binding proteins like recover...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(95)90027-6
更新日期：1995-10-01 00:00:00
abstract：:The non-selective second messenger-gated cation channel TRPC6 (transient receptor potential canonical 6) is activated by diacylglycerols (DAG) in a PKC-independent manner and plays important roles in a variety of physiological processes and diseases. In order to facilitate novel therapies, the development of potent in...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.12.009
更新日期：2019-03-01 00:00:00
abstract：:The annexins are a family of calcium-binding proteins. Data from protein and cDNA sequencing have shown that at least five distinct but closely related mammalian annexins exist each of which possesses four or eight homologous internal repeats which may be calcium-and phospholipid-binding domains. The proteins are pres...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/0143-4160(89)90038-9
更新日期：1989-01-01 00:00:00
abstract：:Agonist induced increases in intracellular free calcium, [Ca2+]i, were measured in single Fura-2 loaded bovine aortic endothelial (BAE) cells by dual wavelength microspectrofluorimetry. Low doses of ATP (less than 10 microM) induced complex changes in [Ca2+]i. These changes usually consisted of a large initial transie...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90011-g
更新日期：1992-04-01 00:00:00
abstract：:Dendritic Ca2+ plays an important role not only in synaptic integration and synaptic plasticity, but also in dendritic excitability in midbrain dopamine neurons. However, the functional organization of dendritic Ca2+ signals in the dopamine neurons remains largely unknown. We therefore investigated dendritic Ca2+ sign...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2011.06.007
更新日期：2011-10-01 00:00:00
abstract：:We have examined the internal Ca(2+)-dependence of the long-term evolution of whole cell high voltage activated Ca current in chromaffin cells. The evolution of the peak Ca current was characterized by 2 distinct phases: after an initial facilitation, there followed a rundown, which represented a reduction by 70% with...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90029-9
更新日期：1994-11-01 00:00:00
abstract：:Apparent calcium stability constants and the purity of Ca-chelating agents were evaluated using Ca(2+)-selective electrodes by the double-log optimization method [Oiki S. Yamamoto T. Okada Y. (1994) Cell Calcium, 15, 199-208]. The method was amended to allow evaluation of the free Ca2+ concentrations contaminating ele...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(94)90060-4
更新日期：1994-03-01 00:00:00
abstract：:An important challenge in the study of Ca2+ signalling is to understand the dynamics of intracellular Ca2+ levels during and after physiological stimulation. While extensive information is available regarding the structural and biophysical properties of Ca2+ channels, pumps and exchangers that control cellular Ca2+ mo...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1054/ceca.2000.0172
更新日期：2000-11-01 00:00:00
abstract：:Cell survival is promoted by the oncoprotein Bcl-2. Previous studies have established that one of the pro-survival actions of Bcl-2 is to reduce cellular fluxes of Ca2+ within cells. In particular, Bcl-2 has been demonstrated to inhibit the release of Ca2+ from the endoplasmic reticulum. However, the mechanism by whic...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2008.01.003
更新日期：2008-09-01 00:00:00
abstract：:We have constructed a stochastic model of the inositol 1,4,5-trisphosphate receptor-Ca2+ channel that is based on quantitative measurements of the channel's properties. It displays the observed dependence of the open probability of the channel with cytosolic [Ca2+] and [IP3] and gives values for the dwell times that a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2003.10.004
更新日期：2004-05-01 00:00:00
abstract：:It is well established that intracellular calcium ([Ca2+]i) controls the inotropic state of the myocardium, and evidence mounts that a "Ca2+ clock" controls the chronotropic state of the heart. Recent findings describe a calcium-activated nonselective cation channel (NSCCa) in various cardiac preparations sharing hall...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2013.06.003
更新日期：2013-09-01 00:00:00
abstract：:K+-dependent Na+-Ca2+ exchangers (NCKXs) belong to Ca2+/cation antiporter gene superfamily. NCKX proteins play an important role in Ca2+ homeostasis and are bi-directional plasma membrane Ca2+-transporters which utilize the inward Na+ and outward K+ gradients to move Ca2+ ions into and out of the cytosol (4Na+:1Ca2+ +...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2018.06.002
更新日期：2018-09-01 00:00:00
abstract：:Cultured rat cerebellar astrocytes, loaded with the Ca2+-sensitive fluorescent dyes Fura-2 or Fluo-3, responded with cytoplasmic Ca2+ transients, when the external K+ concentration was reduced from 5 mM to below 1 mM. Ca2+ transients were generated after changing to a saline containing 0.2 mM K+ in 82% of the cells (n...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2000.0153
更新日期：2000-10-01 00:00:00
abstract：:We present a new method for ratiometric Ca2+ measurements using indicators with excitation spectra in the visible range of wavelengths. Laser-scanning confocal microscopy was used to record intracellular Ca(2+)-signals with high temporal and spatial resolution in single cardiac myocytes. The patch-clamp technique was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(93)90040-d
更新日期：1993-05-01 00:00:00
abstract：:Mechanically induced channel activities and increase of intracellular calcium ([Ca2+]i) in normal and cancerous murine mammary cells (MMT 060562) were investigated using the patch clamp technique and Fura-2 fluorescence. Both cell types showed similar properties. Upon mechanical stimulation, activation of the Ca(2+)-d...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90018-n
更新日期：1992-08-01 00:00:00
abstract：:Cardiogenesis depends on a tightly regulated balance between proliferation and differentiation of cardiac progenitor cells (CPCs) and their cardiomyocyte descendants. While exposure of early mouse embryos to Ca(2+) channel antagonists has been associated with abnormal cardiac morphogenesis, less is known about the con...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.02.018
更新日期：2014-05-01 00:00:00
abstract：:The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(83)90024-6
更新日期：1983-12-01 00:00:00
abstract：:To determine intracellular Ca2+ concentrations more accurately, we examined Kd of Fura-2 for Ca2+ in conditions which were systemically changed. In a solution comprising of 150 mM KCI, 20 mM MOPS-KOH, pH 6.94, 60-100 microM EGTA and 1 microM Fura-2, Kd at 20 degrees C was 0.266 +/- 0.016 microM (mean +/- SEM) (21 dete...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(91)90082-p
更新日期：1991-01-01 00:00:00
abstract：:Calcium and calcium-dependent systems have been long implicated in the induction of epilepsy. We have previously observed that intracellular calcium ([Ca2+]i) levels remain elevated in cells undergoing epileptogenesis in the hippocampal neuronal culture (HNC) model. In this study, we employed the hippocampal neuronal ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2000.0146
更新日期：2000-09-01 00:00:00
abstract：:In the complex signalling interplay that allows extracellular signals to be decoded into activation of apoptotic cell death, Ca(2+) plays a significant role. This is supported not only by evidence linking alterations in Ca(2+) homeostasis to the triggering of apoptotic (and in some cases necrotic) cell death, but also...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143416002002014
更新日期：2002-11-01 00:00:00
abstract：:The effects of the unsaturated fatty acids, arachidonic and oleic acid, on the influx of Ca2+ activated by depletion of intracellular stores with thapsigargin were investigated in various cell types. By using a Ca2+ free/Ca2+ reintroduction protocol, we observed that arachidonic acid (2 to 5 microM) inhibited thapsiga...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90031-2
更新日期：1997-05-01 00:00:00
abstract：:Several potentially life-threatening cardiovascular and respiratory disorders result in prolonged deprivation of oxygen, which in turn results in significant cellular adaptation, or remodelling. An important component of this functional adaptation arises as a direct consequence of altered ion channel expression by chr...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.02.005
更新日期：2004-09-01 00:00:00
abstract：:Ca(2+) influx through voltage-gated Ca(2+) channels triggers the release of neurotransmitters at presynaptic terminals. Some sensory receptor cells in the peripheral auditory and visual systems have specialized synapses that express an electron-dense organelle called a synaptic ribbon. Like conventional synapses, ribb...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.06.004
更新日期：2012-09-01 00:00:00
