Abstract：
:Intercellular Ca(2+)-signaling, after mechanical stimulation of calf pulmonary artery endothelial cells (CPAE), was investigated with fluorescence video imaging. Mechanical stimulation evoked an intracellular Ca(2+)-response in the mechanically stimulated (MS) cell, proceeding to the neighboring (NB) cells as a Ca(2+)-wave. The intercellular propagation of the Ca(2+)-wave was unaffected by the gap junction blockers halothane or heptanol. Therefore the intercellular communication (IC) pathway of the Ca(2+)-wave in CPAE cells does not depend on gap junctional communication but is most likely mediated by release of an extracellular mediator. Continuous unilateral flow experiments confirmed the presence of a diffusible mediator: the Ca(2+)-rise in upstream NB cells is significantly lower than in control experiments. After desensitization of purinergic receptors by pretreatment of CPAE cells with ATP (100mM), UTP (100 microM), 2MeSATP (100microM) or ADPbS (100 microM), the propagation of the intercellular Ca(2+)-wave upon mechanical stimulation was significantly inhibited. Also suramin (200 and 400 microM), a non-specific purinergic receptor blocker, reduced the IC. Application of the nucleotidase apyrase VI (10U/ml), which has a high ATPase/ADPase ratio, enhanced Ca(2+)-signaling and IC. In contrast, apyrase VII (10U/ml), which has a high ADPase/ATPase ratio, significantly depressed the propagation of the intercellular Ca(2+)-wave upon mechanical stimulation. Our experiments therefore demonstrate that the IC, evoked by a mechanical stimulus of CPAE cells, is mediated via release of nucleotides in the extracellular space. The data indicate that the diffusible messenger, responsible for the propagation of a Ca(2+)-wave, is mainly ADP or a combination of ADP/ATP.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Moerenhout M,Himpens B,Vereecke Jdoi
10.1054/ceca.2000.0165keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
125-36issue
2eissn
0143-4160issn
1532-1991pii
S0143-4160(00)90165-9journal_volume
29pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract：:Synaptic transmission is a key signaling event, whereby an action potential-induced release of chemical neurotransmitters again generates a positive or negative electrical activity via opening of postsynaptic channels. Thereafter, information spreads through space, from the postsynaptic membranes to the dendrites, to ...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(98)90113-0
更新日期：1998-02-01 00:00:00
abstract：:Fura-2 fluorescence imaging was used to measure changes in intracellular Ca2+ concentration in individual N1E-115 neuroblastoma cells during repeated activation of M1 muscarinic receptors with carbachol. Ca2+ transients could be elicited repeatedly at 4 min intervals with little decrement as long as external Ca2+ was ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90113-x
更新日期：1996-05-01 00:00:00
abstract：:The Solute Carrier Family 24 (SLC24) belongs to the CaCA super family of Ca(2+)/cation antiporters and codes for five different K(+)- dependent Na(+)- Ca(2+) exchangers (NCKX1-5). NCKX proteins play a critical role in Ca(2+) homeostasis in a wide variety of biological processes such as vision, olfaction, enamel format...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.11.001
更新日期：2016-01-01 00:00:00
abstract：:Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/s0143-4160(03)00143-x
更新日期：2003-10-01 00:00:00
abstract：:In skeletal muscle excitation-contraction (EC) coupling the sarcolemmal L-type Ca(2+) channel or 1,4-dihydropyridine receptor (DHPR) transduces the membrane depolarization signal to the sarcoplasmic Ca(2+) release channel RyR1 via protein-protein interaction. While it is evident that the pore-forming and voltage-sensi...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.04.003
更新日期：2010-06-01 00:00:00
abstract：:STIM and Orai isoforms orchestrate store operated Ca2+ entry (SOCE) and thus cytosolic Ca2+ fluctuations following stimulation by hormones, growth factors and further mediators. Orai1 is a target of Nedd4-2, an ubiquitin ligase preparing several plasma membrane proteins for degradation. Phosphorylation of Nedd4-2 by t...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2012.05.005
更新日期：2012-11-01 00:00:00
abstract：:Aging is a complex process that differentially impacts multiple cognitive, sensory, neuronal and molecular processes. Technological innovations now allow for parallel investigation of neuronal circuit function, structure and molecular composition in the brain of awake behaving adult mice. Thus, mice have become a crit...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2021.102358
更新日期：2021-01-23 00:00:00
abstract：:Using the low-affinity fluorescent Ca(2+) indicators, Mag-Fura-2 and Mag-Fura Red, we studied light- and InsP(3)-induced Ca(2+) release in permeabilized microvillar photoreceptors of the medicinal leech, Hirudo medicinalis. Two major components of the phosphoinositide signaling pathway, phospholipase-C and the InsP(3)...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.2001.0195
更新日期：2001-05-01 00:00:00
abstract：:The strength of the heart beat depends on the amplitude and time course of the transient increase in [Ca(2+)] in the myocytes with each cycle. [Na(+)](i) modulates cardiac contraction through its effect on the Ca(2+) flux through the Na/Ca exchanger. Cardiac excitation-contraction coupling has been postulated to occur...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2004.01.014
更新日期：2004-06-01 00:00:00
abstract：:Cellular senescence is a stable cell proliferation arrest induced by a variety of stresses including telomere shortening, oncogene activation and oxidative stress. This process plays a crucial role in many physiopathological contexts, especially during aging when cellular senescence favors development of age-related d...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1016/j.ceca.2017.04.001
更新日期：2018-03-01 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellu...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2015.04.006
更新日期：2015-08-01 00:00:00
abstract：:Calcium dependence of bleb formation and cell death was evaluated in rat hepatocytes following ATP depletion by metabolic inhibition with KCN and iodoacetate ('chemical hypoxia'). Cytosolic free Ca2+ was measured in single cells by ratio imaging of Fura-2 fluorescence using multiparameter digitized video microscopy. C...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(88)90004-8
更新日期：1988-12-01 00:00:00
abstract：:Secretory granules of pancreatic β-cells contain high concentrations of Ca2+ ions that are co-released with insulin in the extracellular milieu upon activation of exocytosis. As a consequence, an increase in the extracellular Ca2+ concentration ([Ca2+]ext) in the microenvironment immediately surrounding β-cells should...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2012.01.002
更新日期：2012-05-01 00:00:00
abstract：:An inward current responsible for hormone regulated Ca2+ entry has been identified in cultured rat hepatocytes using whole cell patch clamp. Addition of 20 nM vasopressin or of 100 microM ATP induced the inward current, which could be observed more clearly after blocking an outward K+ current. This large outward K+ cu...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90042-x
更新日期：1995-07-01 00:00:00
abstract：:MAP kinases are a family of protein kinases that are ubiquitously expressed and play roles in most signal transduction pathways. They are activated within protein kinase cascades consisting of at least three kinases acting in series. In many, if not all cases, the three-kinase cascade, conveniently referred to as a MA...
journal_title：Cell calcium
pub_type： 杂志文章,评审
doi：10.1054/ceca.1999.0074
更新日期：1999-11-01 00:00:00
abstract：:Intracytoplasmic sperm injection (ICSI) into mammalian eggs induces repetitive rises in intracellular Ca2+ concentration ([Ca2+]i) which are the pivotal signal in fertilization. Spatiotemporal aspects of [Ca2+]i rises following ICSI into the periphery of mouse eggs were investigated with high-speed confocal microscopy...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1054/ceca.1999.0053
更新日期：1999-07-01 00:00:00
abstract：:The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(96)90049-4
更新日期：1996-07-01 00:00:00
abstract：:The actions of the novel calcium (Ca2+) channel antagonist mibefradil (Ro 40-5967), a selective T-type channel blocker in myocardium, were investigated in embryonic rat spinal motoneurones maintained in culture. Whole-cell currents were recorded with the patch-clamp technique. Motoneurones displayed transient, low-vol...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90068-3
更新日期：1997-10-01 00:00:00
abstract：:The Ca2+-sensitive dye quin-2 was used to measure the cytosolic free Ca2+ (Cai2+) in suspensions of ventricular myocytes isolated from cat and rat ventricles. Following an isolation procedure that was similar for both species, the cells were loaded with quin-2 AM (25 microM) for 30 min at 37 degrees C. After two washe...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(87)90002-9
更新日期：1987-08-01 00:00:00
abstract：:Inhibition by Mg2+ ions of exocytotic secretion from permeabilised eosinophils, stimulated by Ca2+ and GTP gamma S, and in the presence and absence of ATP, has been examined. While Mg2+ inhibits release of aryl sulphatase, hexosaminidase and peroxidase, we found no evidence that this occurs by competition at a Ca(2+)-...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(97)90045-2
更新日期：1997-03-01 00:00:00
abstract：:A possible role of the nitric oxide (NO)/cGMP pathway in the regulation of Ca2+ entry into HT29/B6 human colonic epithelial cells was investigated using digital image processing of Fura-2 fluorescence and immunoblotting for nitric oxide synthase (NOS). We tested the hypothesis that Ca2+ store depletion causes increase...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(95)90071-3
更新日期：1995-04-01 00:00:00
abstract：:The effects of acute (3 h) and chronic (30 h) in vivo infusions of Escherichia coli endotoxin on the Ca2+ homeostasis of rat spleen cells was investigated. Conditions were established for obtaining reliable estimates of [Ca2+]i in these cells using the newly-developed Ca2+ indicator Fluo-3. The resting [Ca2+]i of sple...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/0143-4160(92)90005-d
更新日期：1992-02-01 00:00:00
abstract：:Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the alpha-subunit of Gq (Ad-EF1-dnG alpha q) and the other expressing the wild-type alpha-subunit of Gq (Ad-EF1-wtG ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(98)90077-x
更新日期：1998-08-01 00:00:00
abstract：:Ca2+ entry through voltage-gated Ca2+ channels (VGCC) triggers transmitter release. Direct recording of Ca2+ currents from the calyx of Held nerve terminal revealed that presynaptic VGCCs undergo various modulations via presynaptic G protein-coupled receptors (GPCRs), Ca2+-binding proteins and a developmental switch o...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2005.01.018
更新日期：2005-05-01 00:00:00
abstract：:Sarcoplasmic reticulum contains the internal Ca(2+) store in smooth muscle cells and its lumen appears to be a continuum that lacks diffusion barriers. Accordingly, the free luminal Ca(2+) level is the same all throughout the SR; however, whether the Ca(2+) buffer capacity is the same in all the SR is unknown. We have...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2009.07.005
更新日期：2009-09-01 00:00:00
abstract：:Cyclic AMP regulates the late step of Ca²+-dependent exocytosis in many secretory cells through two major mechanisms: a protein kinase A-dependent and a cAMP-GEF/Epac-dependent pathway. We designed a protocol to characterize the role of these two cAMP-dependent pathways on the Ca²+ sensitivity and kinetics of regulate...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2010.12.005
更新日期：2011-02-01 00:00:00
abstract：:Intracellular calcium signals activated by growth factors in endothelial cells during angiogenesis regulate cytosolic and nuclear events involved in survival, proliferation and motility. Among the intracellular messengers released after proangiogenic stimulation (bFGF, VEGF), arachidonic acid (AA), nitric oxide (NO) a...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2006.07.003
更新日期：2007-03-01 00:00:00
abstract：:We report here the first local and global Ca(2+) measurements made from in situ terminal arterioles. The advantages of the method are that there is minimal disturbance to the vessels, which retain their relationship to the tissue they are supplying (rat ureter) and the small size of vessel that can be studied. Good lo...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/s0143-4160(03)00019-8
更新日期：2003-07-01 00:00:00
abstract：:Cytosolic Ca(2+) signals are fundamental for the early and late steps of myoblast differentiation and are, as in many cells, generated by Ca(2+) release from internal stores as well as by plasma membrane Ca(2+) entry. Our recent studies identified the store-operated Ca(2+) channels, Orai1 and TRPC1&C4, as crucial for ...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.10.014
更新日期：2014-12-01 00:00:00
abstract：:Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein...
journal_title：Cell calcium
pub_type： 杂志文章
doi：10.1016/j.ceca.2014.05.003
更新日期：2014-08-01 00:00:00
