Abstract:
:The Solute Carrier Family 24 (SLC24) belongs to the CaCA super family of Ca(2+)/cation antiporters and codes for five different K(+)- dependent Na(+)- Ca(2+) exchangers (NCKX1-5). NCKX proteins play a critical role in Ca(2+) homeostasis in a wide variety of biological processes such as vision, olfaction, enamel formation, Melanocortin-4-receptor-dependent satiety and skin pigmentation. NCKX transcripts are widely found throughout the brain. In this study we examine the differences between NCKX1-4 in terms of cation dependencies. We measured changes to Ca(2+) influx via the reverse exchange mode while manipulating external Ca(2+) or K(+) or internal Na(+) concentrations (External Ca(2+) Dependence, External K(+) Dependence and Internal Na(+) Dependence respectively); we also looked at the effect of external Na(+)/Ca(2+) competition and 3' 4'-Dichlorobenzamil on the transport of ions in HEK 293 cell lines. A fluorescence based assay was used to determine differences in transport kinetics of the four membrane spanning exchangers using the Michaelis-Menten constant (Km). Our results show that there are no significant differences between the NCKX isoforms to explain the variation in the specific expression pattern of these exchangers.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Jalloul AH,Szerencsei RT,Schnetkamp PPdoi
10.1016/j.ceca.2015.11.001subject
Has Abstractpub_date
2016-01-01 00:00:00pages
1-11issue
1eissn
0143-4160issn
1532-1991pii
S0143-4160(15)00170-0journal_volume
59pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::Several proteins have been identified that associate with calcium release channels and potentially regulate their function. Using tacrolimus as a pharmacological tool, we investigated whether the immunophilin FKBP12 modulates ryanodine receptor channels in intestinal smooth muscle. Results with PCR demonstrated the pr...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(97)90078-6
更新日期:1997-12-01 00:00:00
abstract::Ca(2+) is known as a universal messenger mediating a wide variety of cellular processes, including cell death. In fact, this ion has been proposed as the 'cell death master', not only at the intracellular but also at the intercellular level. The most direct form of intercellular spread of cell death is mediated by gap...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.05.007
更新日期:2011-09-01 00:00:00
abstract::Although store-operated calcium entry (SOCE) was identified more that two decades ago, understanding the molecular mechanisms that regulate and mediate this process continue to pose a major challenge to investigators in this field. Thus, there has been major focus on determining which of the models proposed for this m...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.01.013
更新日期:2007-08-01 00:00:00
abstract::Isolated avian growth plate chondrocytes convert the acetoxymethyl ester (AM) form of Fura-2 quickly and efficiently to the Ca2(+)-sensitive pentacarboxylic acid (FA) form. Control experiments indicate that the Kd for intracellular Fura-2/FA is very close to that of extracellular Fura-2/FA at the same ionic strength a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90077-8
更新日期:1990-08-01 00:00:00
abstract::The sternal epithelium of Porcellio scaber was used as a novel model to study the subcellular elemental distribution in control and Ca(2+)-transporting stages in situ. The anterior sternal epithelium (ASE) is specialized for transport of cuticular Ca to sternal CaCO(3) deposits during premolt, and from these deposits ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(02)00060-x
更新日期:2002-06-01 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is a progressive muscle disease with severe cardiac complications. It is believed that cellular oxidative stress and augmented Ca(2+) signaling drives the development of cardiac pathology. Some mitochondrial and metabolic dysfunctions have also been reported. Here we investigate cellu...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.04.006
更新日期:2015-08-01 00:00:00
abstract::Using the fluorescent Ca2+ probe Quin-2 it has been reported that cholera toxin (CT) and its B subunit (B-CT) increase cytosolic free Ca2+ concentration ([Ca2+]i) in entherocytes, thymocytes and fibroblasts. In this work we show, however, that the fluorescence increases of Quin-2-loaded cells (rat thymocytes, mouse sp...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90054-x
更新日期:1990-06-01 00:00:00
abstract::The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2001.0241
更新日期:2001-11-01 00:00:00
abstract::Numerous lines of evidence indicate that nuclear calcium concentration ([Ca2+]n) may be controlled independently from cytosolic events by a local machinery. In particular, the perinuclear space between the inner nuclear membrane (INM) and the outer nuclear membrane (ONM) of the nuclear envelope (NE) likely serves as a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2019.102143
更新日期:2020-03-01 00:00:00
abstract::Indo-1 is a new fluorescent indicator of the intracellular free calcium concentration Cai++. Indo-1 may be used in a similar manner as its predecessor quin2 but offers the principal advantage that the Ca++ saturated form of the Ca++ chelator has a emission maximum different in wavelength from that of free indo-1 (400 ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(86)90003-5
更新日期:1986-08-01 00:00:00
abstract::Relative to mammals, the neuroendocrine control of pituitary growth hormone (GH) secretion and synthesis in teleost fish involves numerous stimulatory and inhibitory regulators, many of which are delivered to the somatotrophs via direct innervation. Among teleosts, how multifactorial regulation of somatotroph function...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.11.001
更新日期:2012-03-01 00:00:00
abstract::A characteristic feature of developing neural networks is spontaneous periodic activity. In the developing retina, retinal ganglion cells fire bursts of action potentials that drive large increases in intracellular calcium concentration with a periodicity of minutes. These periodic bursts of action potentials propagat...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2005.01.010
更新日期:2005-05-01 00:00:00
abstract::It is well known that in different tissues, dihydropyridines bind at nanomolar concentrations to a receptor and block voltage-operated Ca2+ channels. In studies reported here, Harderian gland tissue homogenates from intact male hamsters exhibited significant dihydropyridine binding (Bmax = 1700 fmoles/mg protein) of h...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(92)90036-r
更新日期:1992-10-01 00:00:00
abstract::A whole range of cell functions are regulated by the free cytosolic Ca(2+)concentration. Activator Ca(2+)from the extracellular space enters the cell through various types of Ca(2+)channels and sometimes the Na(+)/Ca(2+)-exchanger, and is actively extruded from the cell by Ca(2+)pumps and Na(+)/Ca(2+)-exchangers. Acti...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1054/ceca.2000.0131
更新日期:2000-07-01 00:00:00
abstract::Calcium ion is involved in diverse physiological and developmental pathways. One of the important roles of calcium is a signaling messenger, which regulates signal transduction in plants. CBL (calcineurin B-like protein) is one of the calcium sensors that specifically interact with a family of serine-threonine protein...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2014.05.003
更新日期:2014-08-01 00:00:00
abstract::Polycystin-2, a member of the TRP family of calcium channels, is encoded by the human PKD2 gene. Mutations in that gene can lead to swelling of nephrons into the fluid-filled cysts of polycystic kidney disease. In addition to expression in tubular epithelial cells, human polycystin-2 is found in muscle and neuronal ce...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2005.03.003
更新日期:2005-06-01 00:00:00
abstract::Receptors for extracellular nucleotides (the P2X-calcium channels and the phospholipase C-coupled P2Y receptors) play key roles in pain signaling, but little is known on their function in trigeminal ganglia, whose hyperactivation leads to the development of migraine pain. Here we characterize calcium signaling via P2X...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.10.003
更新日期:2008-06-01 00:00:00
abstract::The endoplasmic reticulum (ER) acts as a dynamic calcium store and is involved in the generation of specific patterns of calcium signals in neurons. Calcium is mobilized from the ER store by multiple signaling cascades, and neuronal activity is known to regulate ER calcium levels. We asked how neurons regulate ER calc...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.04.002
更新日期:2015-08-01 00:00:00
abstract::The mechanism by which the depletion of intracellular Ca2+ stores stimulates Ca2+ influx is poorly understood. However, the coupling of depletion to influx is broken during mitosis [Preston, S.F. et. al., (1991) Cell Regul., 2, 915-925]. Thus, in interphase HeLa cells, activation of the histamine H1 receptor, or incub...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90042-5
更新日期:1993-05-01 00:00:00
abstract::Depletion of calcium in the extracellular medium used to incubate first trimester human placental minces resulted in a significant decrease in the quantity of immunoreactive hCG in the medium and a corresponding increase in the tissue. In contrast, when secretion of newly synthesised hCG was monitored in the absence o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90060-j
更新日期:1993-09-01 00:00:00
abstract::The best known calcium waves move at about 5-30 microns/s (at 20 degrees C) and will be called fast waves to distinguish them from slow (contractile) ones which move at 0.1-1 microns/s as well as electrically propagated, ultrafast ones. Fast waves move deep within cells and seem to underlie most calcium signals. Their...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(93)90099-r
更新日期:1993-11-01 00:00:00
abstract::An influx of Ca2+ into red blood cells of the bivalve mollusc Noetia ponderosa occurs immediately following a hypo-osmotic stress. The volume recovery response to the stress is dependent upon [Ca2+]o and is inhibited by phenothiazines. The action of these drugs is on the amino acid regulation portion of the recovery r...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(89)90070-5
更新日期:1989-04-01 00:00:00
abstract::N,N'-dicyclohexylcarbodiimide (DCCD) and 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide (CMCD) inhibited calmodulin-dependent Ca2(+)+Mg2(+)-ATPase activity in erythrocyte ghost membranes. The extent of the inhibition caused by carbodiimides strongly depended on their hydrophobicity. Hydrophobic DCCD was a more potent...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90004-e
更新日期:1990-04-01 00:00:00
abstract::The regulation of cytosolic-free calcium concentration of smooth-muscle and endothelial cells was mainly studied on cultured cells where the cross talk between these two coupled cell types is lost. In the present study, the cytosolic-free calcium concentration in the endothelial and the smooth-muscle cells was examine...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2001.0233
更新日期:2001-10-01 00:00:00
abstract::Transient spontaneous increases in the intracellular Ca2+ concentration have been frequently observed in astrocytes in cell culture and in acutely isolated slices from several brain regions. Recent in vivo experiments, however, reported only a low frequency of spontaneous Ca2+ events in astrocytes. Since the ex vivo e...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2007.06.002
更新日期:2008-03-01 00:00:00
abstract::Pacemaker cells residing in the sinoatrial node generate the regular heartbeat. Ca2+ signaling controls the heartbeat rate-directly, through the effect on membrane molecules (NCX exchange, K+ channel), and indirectly, through activation of calmodulin-AC-cAMP-PKA signaling. Thus, the physiological role of signaling in ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2018.12.008
更新日期:2019-03-01 00:00:00
abstract::To characterize the effect that a phosphatidylinositol 3-kinase (PI3-kinase) inhibitor, LY294002, has on cytosolic calcium concentrations ([Ca2+]i), bovine airway smooth muscle cells (BASMC) and cultured human bronchial smooth muscle cells (HBSMC) were loaded with fura 2-AM, imaged as single cells and [Ca2+]i measured...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(02)00111-2
更新日期:2002-07-01 00:00:00
abstract::The sensitivity to Ca2+ of the Ca2+-dependent K+ channel can be increased by the artificial electron donor system ascorbate + phenazine-methosulphate in a variety of animal cells. In the human erythrocyte the shift from the 'low' to the 'high-affinity' state seems to depend on the reduction of a membrane component acc...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90024-6
更新日期:1983-12-01 00:00:00
abstract::We have used single cell fluorescence imaging techniques to examine how functional properties of the caffeine-sensitive Ca(2+) store change during differentiation of a sub-population of caffeine-sensitive SH-SY5Y cells. Application of caffeine (30 mM) 1-10.5 min after a 'priming' depolarisation pulse of 55 mM K(+) rev...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2006.06.007
更新日期:2007-03-01 00:00:00
abstract::The glucoregulatory hormones insulin and glucagon are released from the β- and α-cells of the pancreatic islets. In both cell types, secretion is secondary to firing of action potentials, Ca(2+)-influx via voltage-gated Ca(2+)-channels, elevation of [Ca(2+)](i) and initiation of Ca(2+)-dependent exocytosis. Here we di...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2011.11.006
更新日期:2012-03-01 00:00:00