Abstract:
:Intracytoplasmic sperm injection (ICSI) into mammalian eggs induces repetitive rises in intracellular Ca2+ concentration ([Ca2+]i) which are the pivotal signal in fertilization. Spatiotemporal aspects of [Ca2+]i rises following ICSI into the periphery of mouse eggs were investigated with high-speed confocal microscopy. The first Ca2+ response was generated 25-30 min after ICSI, when [Ca2+]i increased slowly and reached a certain level. The [Ca2+]i rise occurred synchronously over the ooplasm, attained the peak in 40-70 s, and lasted for 5-7 min. Succeeding Ca2+ responses occurred at intervals of 20-30 min, associated with the faster rate of [Ca2+]i rise and the shorter duration as Ca2+ oscillations progressed. The [Ca2+]i rises took the form of a wave that started from an arbitrary cortical region, but not from the vicinity of the injected sperm head. The Ca2+ wave became more pronounced and propagated across the egg faster in the later Ca2+ responses. An artifactual [Ca2+]i rise was inevitably produced during the ICSI procedure. The larger artifact affected the subsequent first Ca2+ response, resulting in the faster [Ca2+]i rise (time to peak, 10-20 s), slight spatial heterogeneity of [Ca2+]i rise in the ooplasm (but not a wave) and the shorter duration (3-4 min). The artifact slightly affected the amplitude of the second Ca2+ response, but little affected the later Ca2+ responses. It is suggested that the factor(s) that leaked out of the injected spermatozoon diffuses to a wide area and sensitizes Ca2+ channels of the endoplasmic reticulum to induce Ca2+ release synchronously over the ooplasm. The enhanced sensitization leads to propagating Ca2+ release initiated from the cortex that is more sensitive to the sperm factor.
journal_name
Cell Calciumjournal_title
Cell calciumauthors
Sato MS,Yoshitomo M,Mohri T,Miyazaki Sdoi
10.1054/ceca.1999.0053keywords:
subject
Has Abstractpub_date
1999-07-01 00:00:00pages
49-58issue
1-2eissn
0143-4160issn
1532-1991pii
S0143-4160(99)90053-2journal_volume
26pub_type
杂志文章相关文献
CELL CALCIUM文献大全abstract::Signaling between intracellular Ca2+ stores and cell membrane channels or transporters is important to Ca(2+)-based second messenger systems. Two hypotheses, the capacitative and the Ca(2+)-induced Ca(2+)-influx models have been proposed to explain aspects of this signaling. In this study, we examined the applicabilit...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90076-0
更新日期:1994-12-01 00:00:00
abstract::We present a new method for ratiometric Ca2+ measurements using indicators with excitation spectra in the visible range of wavelengths. Laser-scanning confocal microscopy was used to record intracellular Ca(2+)-signals with high temporal and spatial resolution in single cardiac myocytes. The patch-clamp technique was ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(93)90040-d
更新日期:1993-05-01 00:00:00
abstract::Here we report on the feasibility of using replication deficient adenoviruses to modify signal transduction systems in epithelia. We constructed two viruses, one expressing a dominant negative mutant of the alpha-subunit of Gq (Ad-EF1-dnG alpha q) and the other expressing the wild-type alpha-subunit of Gq (Ad-EF1-wtG ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(98)90077-x
更新日期:1998-08-01 00:00:00
abstract::We have found that the changes in calcium metabolism in rabbit neutrophils produced by the chemotactic synthetic peptide f-Met-Leu-Phe are not sensitive to the calcium chelator EGTA. The present results demonstrate unambiguously that the previously described chemotactic factor induced changes in 45Ca fluxes in rabbit ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(83)90049-0
更新日期:1983-02-01 00:00:00
abstract::The Ca(2+) and cAMP/PKA pathways are the primary signaling systems in secretory epithelia that control virtually all secretory gland functions. Interaction and crosstalk in Ca(2+) and cAMP signaling occur at multiple levels to control and tune the activity of each other. Physiologically, Ca(2+) and cAMP signaling oper...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2014.01.006
更新日期:2014-06-01 00:00:00
abstract::The mammalian transient receptor potential (TRP) proteins consist of a superfamily of Ca2+-permeant non-selective cation channels with structural similarities to Drosophila TRP. The TRP superfamily can be divided into three major families, among them the "canonical TRP" family (TRPC). The seven protein products of the...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00056-3
更新日期:2003-05-01 00:00:00
abstract::The effect of the uterotonic pituitary hormone oxytocin on the regulation of intracellular calcium concentration ([Ca2+]i) was studied in single cells of a smooth muscle cell line derived from human non-pregnant myometrium. [Ca2+]i was measured with fluorescence microscopy, and by recording the activity of Ca(2+)-acti...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90049-4
更新日期:1996-07-01 00:00:00
abstract::Specific Ca(2+) homeostatic system appeared very early in the history of the cell, as a survival system preventing Ca(2+)-mediated cell damage. This homeostatic system produced a steep ( approximately 20,000 times) concentration gradient between extracellular and intracellular compartments, which has both survival imp...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.05.001
更新日期:2007-10-01 00:00:00
abstract::The lungs as the gateways of our body to the external environment are essential for gas exchange. They are also exposed to toxicants from two sides, the airways and the vasculature. Apart from naturally produced toxic agents, millions of human made chemicals were produced since the beginning of the industrial revoluti...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2017.04.005
更新日期:2017-11-01 00:00:00
abstract::Under certain conditions of Ca2+ loading, cardiac myocytes, both isolated and in intact tissue, exhibit spontaneous, oscillatory Ca2+ transients due to Ca2+ release from the sarcoplasmic reticulum. These transients are not triggered by depolarization of the sarcolemma, though they themselves can generate depolarizing ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(88)90005-x
更新日期:1988-12-01 00:00:00
abstract::Ca(2+) signals are universal among cells in regulating a spectrum of cellular responses. Phospholipase C-coupled receptors activate two components of Ca(2+) signals--rapid Ca(2+) release from ER stores, followed by slower Ca(2+) entry from outside the cell. The coupling process between ER and PM to mediate this "store...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.03.009
更新日期:2007-08-01 00:00:00
abstract::Calcium release-activated calcium (CRAC) channels have been the target of drug discovery for many years. The identification of STIM and Orai proteins as key components of CRAC channels greatly facilitated this process because their co-expression in cell lines produced electrophysiological currents (ICRAC) much larger ...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2018.07.005
更新日期:2018-09-01 00:00:00
abstract::The control of calcium homeostasis is essential for cell survival and is of crucial importance for several physiological functions. The discovery of the epithelial calcium channel Transient Receptor Potential Vaniloid (TRPV6) in intestine has uncovered important Ca(2+) absorptive pathways involved in the regulation of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2008.02.007
更新日期:2008-11-01 00:00:00
abstract::The Solute Carrier Family 24 (SLC24) belongs to the CaCA super family of Ca(2+)/cation antiporters and codes for five different K(+)- dependent Na(+)- Ca(2+) exchangers (NCKX1-5). NCKX proteins play a critical role in Ca(2+) homeostasis in a wide variety of biological processes such as vision, olfaction, enamel format...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.11.001
更新日期:2016-01-01 00:00:00
abstract::Mast cells are considered the primary initiators of allergic diseases as a consequence of the release of multiple inflammatory mediators on activation. Although predominately activated through antigen-mediated aggregation of IgE-occupied-FcɛRI, they can also be induced to release mediators by other receptors and envir...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2011.08.003
更新日期:2011-11-01 00:00:00
abstract::Central to controlling intracellular calcium concentration ([Ca(2+)](i)) are a number of Ca(2+) transporters and channels with the L-type Ca(2+) channel, Na(+)-Ca(2+) exchanger and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) being of particular note in the heart. This review concentrates on the regulation of [Ca(2+)]...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2007.04.002
更新日期:2007-10-01 00:00:00
abstract::Localized subcellular changes in Ca(2+) serve as important cellular signaling elements, regulating processes as diverse as neuronal excitability and gene expression. Studies of cellular Ca(2+) signaling have been greatly facilitated by the availability of fluorescent Ca(2+) indicators. The respective merits of differe...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2015.10.003
更新日期:2015-12-01 00:00:00
abstract::P2Y(12) antagonists such as clopidogrel and AR-C69931MX inhibit aggregation by antagonizing the effects of ADP at P2Y(12) receptors on platelets. Agents such as PGE(1) also inhibit aggregation by stimulating adenylate cyclase to produce cAMP, which interferes with Ca(2+) mobilization within the cell. Since one facet o...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00170-2
更新日期:2004-01-01 00:00:00
abstract::Isolated avian growth plate chondrocytes convert the acetoxymethyl ester (AM) form of Fura-2 quickly and efficiently to the Ca2(+)-sensitive pentacarboxylic acid (FA) form. Control experiments indicate that the Kd for intracellular Fura-2/FA is very close to that of extracellular Fura-2/FA at the same ionic strength a...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(90)90077-8
更新日期:1990-08-01 00:00:00
abstract::The calcium release channel/ryanodine receptor of rabbit skeletal muscle sarcoplasmic reticulum is tightly associated with the immunophilin FK-506 binding protein (FKBP-12). The immunosuppressant drug FK-506 effectively dissociates FKBP-12 from the calcium release channel of terminal cisternae (TC) vesicles. Furthermo...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(94)90048-5
更新日期:1994-02-01 00:00:00
abstract::Disturbances in intracellular Ca2+ are known to be important in cell injury caused by a wide range of toxic factors. The complement system is a major effector of immune damage in vivo, and is known to be involved in the pathogenesis of many immune diseases. We present here evidence that the potentially lethal membrane...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/0143-4160(86)90042-4
更新日期:1986-12-01 00:00:00
abstract::Human neutrophils, preloaded with the fluorescent probe, Fura-2, were exposed to Ca2+-releasing agents. The monitored traces of fluorescence were transformed by computer to cytosolic Ca2+ concentration ([ Ca2+]i). Due to quenching of Fura-2, the addition of Mn2+ enabled us to compute the cytosolic concentration of tot...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(89)90025-0
更新日期:1989-10-01 00:00:00
abstract::CatSper is a sperm-specific Ca(2+) channel that plays an essential role in the male fertility. However, its biophysical properties have been poorly characterized mainly due to its deficient heterologous expression. As other voltage-gated Ca(2+) channels (CaVs), CatSper possesses a conserved Ca(2+)-selective filter mot...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2016.03.011
更新日期:2016-07-01 00:00:00
abstract::The tissue/medium distribution of the nonmetabolized glucose analog [14C]-3-O-methyl-D-glucose was measured in pigeon erythrocytes and related to changes in 45Ca uptake and efflux, total calcium content and ATP levels. Sugar transport was not affected by changes in external Ca2+. However, both sugar and 45Ca influx we...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/0143-4160(82)90004-5
更新日期:1982-08-01 00:00:00
abstract::Capacitative calcium entry is a process whereby the depletion of Ca(2+) from intracellular stores (likely endoplasmic or sarcoplasmic reticulum) activates plasma membrane Ca(2+) channels. Current research has focused on identification of capacitative calcium entry channels and the mechanism by which Ca(2+) store deple...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/s0143-4160(03)00143-x
更新日期:2003-10-01 00:00:00
abstract::This investigation concentrates on the change in Ca(2+) concentration ([Ca(2+)]) caused by ryanodine in U373 MG cells. This cell type from a human astrocytoma is a unique cellular model because it only expresses the type 3 ryanodine receptor (RyR3), which is generally the least abundant isoform. In the presence of phy...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/j.ceca.2004.02.004
更新日期:2004-11-01 00:00:00
abstract::Three decades ago, James W. Putney Jr. conceptualized the idea of store-operated calcium entry (SOCE) to explain how depletion of endoplasmic reticulum (ER) Ca2+ stores evokes Ca2+ influx across the plasma membrane. Since the publication of this highly influential idea, it is now established that SOCE is universal amo...
journal_title:Cell calcium
pub_type: 杂志文章,评审
doi:10.1016/j.ceca.2016.12.006
更新日期:2017-05-01 00:00:00
abstract::Vesl/Homer proteins physically link proteins that mediate cellular signaling [Curr. Opin. Neurobiol. 10 (2000) 370; Trends Neurosci. 23 (2000) 80; J. Cell Sci. 113 (2000) 1851] and thereby influence cellular function [Nat. Neurosci. 4 (2001) 499; Nature 411 (2001) 962]. A previous study reported that Vesl-1L/Homer-1c ...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(03)00082-4
更新日期:2003-08-01 00:00:00
abstract::Agonist-induced calcium (Ca) mobilization is accompanied by Ca efflux, presumably reflecting the rise in Ca concentration at the cytosolic surface of the cell membrane. We studied the relationship between Ca efflux and intracellular Ca mobilization in Xenopus oocytes. Elevation of cytosolic Ca by a direct injection of...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1016/s0143-4160(96)90021-4
更新日期:1996-03-01 00:00:00
abstract::The lack of specific inhibitors of the plasma membrane Ca2+ pump (PMCA) has made vanadate (VO3-), a non-specific inhibitor, an invaluable tool in the study of PMCA function. However, three important properties of vanadate as an inhibitor of the PMCA in intact cells, namely its speed of action in different experimental...
journal_title:Cell calcium
pub_type: 杂志文章
doi:10.1054/ceca.2001.0241
更新日期:2001-11-01 00:00:00